• Journal of Environmental Health Sciences
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• v.40 no.2
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• pp.114-126
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• 2014

Objectives: We aimed to develop a measurement method of five metabolites of trichloroethylene (TCE) in a concurrent biological sample, e.g., trichloroacetic acid (TCA), dichloroacetic acid (DCA), S-(1,2-dichlorovinyl) glutathione (DCVG), S-(1,2-dichlorovinyl)-L-cysteine (DCVC), and N-Acetyl-S-(1,2-dichlorovinyl)-L-cysteine (NAcDCVC) and to validate the method before application to pharmacokinetic study. Methods: TCE metabolites were simultaneously analyzed using high performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) with as little as 50 ${\mu}L$ of serum and urine. DCA, TCA and NAcDCVC were extracted with diethyl ether, while DCVC and DCVG were extracted by solid phase extraction. This method was validated according to the guidelines for bioanalytical method validation of the Korean National Institute of Toxicological Research. Then, we determined the five metabolites in five strains of mice at 24 hr after exposure to 1 g TCE /kg body weight. Results: The limits of detection for the five metabolites in biological samples ranged from 0.001 to 0.076 nmol/mL, which is comparable to or better than those previously reported. Most calibration curves showed good linearity ($R^2=0.99$), and between-batch variation was less than 20% expressing acceptable robustness and reproducibility. Using this method, we found TCA and DCA were detected in all test mice at 24 hr after the oral administration while NAcDCVC and DCVC were detected in some strains, which showed strain-dependent metabolism of TCE. Conclusions: The present method could provide robust and accurate measurements of major key metabolites of TCE in biological media, which allowed concurrent analysis of TCE metabolism for limited amounts of biospecimens.

• The Journal of Korean Society of Virology
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• v.28 no.4
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• pp.347-358
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• 1998

To analyze the correlation between biological phenotypes of HIV-1 isolates and disease progression, we selected 9 long-term non-progressors (LTNP) and 12 rapid progressors (RP) from HIV-1 infected Korean. We isolated HIV-1 isolates by culture of PBMC of LTNP and RP with normal PBMC and measured HIV-1 p24 antigen production. The HIV-1 isolation rate from LTNP was 55.6% (5/9). And 4 HIV-1 LTNP isolates were non-syncytium inducing (NSI) phenotype and showed slow/low replication. The HIV-1 isolation rate from RP was 91.7% (11/12) which was higher than that from LTNP. Besides 3 RP HIV-1 isolates which showed syncytium inducing (SI) phenotype, 8 RP HIV-1 isolates showed NSI phenotype in normal PBMC and MT-2 cell line. All RP HIV-1 isolates replicated more rapidly than LTNP HIV-1 isolates. Comparing the replication kinetics and syncytium forming capacity of HIV-1 isolates from LTNP and RP, we suggest that the difference of biological phenotype of HIV-1 isolates could be related with disease progression of HIV-1 infected persons.

• Journal of environmental and Sanitary engineering
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• v.23 no.3
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• pp.23-30
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• 2008

This study was carried out to get efficient of nutrient removal effects on the treatment of paper making wastewater using submerged biological film process. The concentration of average BOD at raw wastewater was $324mg/{\ell}$ and COD was $435mg/{\ell}$. The average BOD of effluent was maintained $24.2mg/{\ell}$ and average COD was $37mg/{\ell}$. The concentration of average T-N at raw wastewater was $16.5mg/{\ell}$ and T-P was $1.2mg/{\ell}$. The average T-P of effluent was maintained $2.3mg/{\ell}$ and average T-P was $0.08mg/{\ell}$. The concentration of average SS at influent wastewater was $263mg/{\ell}$. The average SS of effluent was maintained $28.2mg/{\ell}$. The result of this experiment was wastage sludge did generated and the removal efficiency of nutrients were kept equilibrium.

• Journal of Nutrition and Health
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• v.55 no.1
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• pp.1-9
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• 2022

In the past few decades, great progress has been made on understanding the interaction between nutrition and health status. But despite this wealth of knowledge, health problems related to nutrition continue to increase. This leads us to postulate that the continuing trend may result from a lack of consideration for intra-individual biological variation on dietary responses. Precision nutrition utilizes personal information such as age, gender, lifestyle, diet intake, environmental exposure, genetic variants, microbiome, and epigenetics to provide better dietary advices and interventions. Recent technological advances in the artificial intelligence, big data analytics, cloud computing, and machine learning, have made it possible to process data on a scale and in ways that were previously impossible. A big data platform is built by collecting numerous parameters such as meal features, medical metadata, lifestyle variation, genome diversity and microbiome composition. Sophisticated techniques based on machine learning algorithm can be used to integrate and interpret multiple factors and provide dietary guidance at a personalized or stratified level. The development of a suitable machine learning algorithm would make it possible to suggest a personalized diet or functional food based on analysis of intra-individual metabolic variation. This novel precision nutrition might become one of the most exciting and promising approaches of improving health conditions, especially in the context of non-communicable disease prevention.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2003.11a
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• pp.11-14
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• 2003

Adipocyte development has an impact on human health issues mainly through the association of obesity with Type II diabetes, hyperlipidemia, hypertension and cardiovascular disorders. Obesity results from excessive food intake and reduced energy expenditure, and such surplus energy is stored in adipose tissue.

• Journal of Korean Biological Nursing Science
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• v.2 no.1
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• pp.34-48
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• 2000

The purpose of this study was to analyse the factors associated with regular exercise in office workers based on the health belief model such as health belief, self efficacy and barrier among office workers. For the study, we surveyed 253 office workers and analysed using SAS package program. That results obtained were as follows; 1. There were significant differences in sex, age, marriage status. 2. Exercise showed a significant association according to 5-6 days/week exercise, used to fitness center in company, exercise before the attendances. 3. The health belief showed a significant association according to 20-29 years old, unmarried and non exerciser. 4. The self efficacy showed a significant association according to female, nondrinker, nonsmoker, regular exerciser, motivation of exercise is maintain health and weak exerciser. 5. The barrier of exercise was high in non exerciser. 6. A negative correlation was observed between the health belief, the self efficacy and the barrier of exercise. In conclusion, this study suggests that the effective exercise program to reinforce the factors based on health belief and self efficacy, have to be developed.

• Journal of Preventive Medicine and Public Health
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• v.47 no.5
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• pp.245-252
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• 2014

Arsenic is a unique element with distinct physical characteristics and toxicity whose importance in public health is well recognized. The toxicity of arsenic varies across its different forms. While the carcinogenicity of arsenic has been confirmed, the mechanisms behind the diseases occurring after acute or chronic exposure to arsenic are not well understood. Inorganic arsenic has been confirmed as a human carcinogen that can induce skin, lung, and bladder cancer. There are also reports of its significant association to liver, prostate, and bladder cancer. Recent studies have also suggested a relationship with diabetes, neurological effects, cardiac disorders, and reproductive organs, but further studies are required to confirm these associations. The majority of research to date has examined cancer incidence after a high exposure to high concentrations of arsenic. However, numerous studies have reported various health effects caused by chronic exposure to low concentrations of arsenic. An assessment of the health effects to arsenic exposure has never been performed in the South Korean population; thus, objective estimates of exposure levels are needed. Data should be collected on the biological exposure level for the total arsenic concentration, and individual arsenic concentration by species. In South Korea, we believe that biological exposure assessment should be the first step, followed by regular health effect assessments.

• Journal of Korean Biological Nursing Science
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• v.21 no.4
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• pp.308-317
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• 2019

Purpose: This study aimed to investigate the level and related factors related to health-promoting lifestyle in operating room nurses in Korea. Method: A cross-sectional descriptive survey design was employed. The data were collected using questionnaire for three weeks in December 2018 from 110 operating room nurses working for more than six months in a general hospital located in Seoul. The Health Promoting Lifestyle Profile-II, The Korean Version of the Practice Environment Scale of Nursing Work Index, and Operating nurse's Job stress Factor Scale were used. Results: Significant relationships were observed between health promoting lifestyle and educational level(p= .025), perceived economic status (p= .001) wearing a lead apron for protection from radiation during the operation(p< .001), work satisfaction(p= .016), and fatigue related to work(p= .006). Also significant correlations were identified between nursing work environment and health promoting lifestyle. However, the health promoting lifestyle was not statistically different based on occupational stress(p= .365). In multiple linear regression analyses, the level of health promoting lifestyle found to be higher in subjects who did not wear a lead apron for protection from radiation(p= .017), and who had more positive perception of the nursing work environment(p= .034). Conclusion: In order to increase health promoting lifestyle of operating nurses, the strategies to improve the nursing work environment are essential.

• Journal of Korean Biological Nursing Science
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• v.21 no.2
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• pp.152-159
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• 2019

Purpose: To understand awareness, attitude and knowledge levels of muscle health of adults over 18 years old. Methods: This study was a cross-sectional study using questionnaires. A total of 401 questionnaires were included for final analysis. Mean and standard deviation of the continuous variables were analyzed and frequency analysis of categorical variables was performed. To identify differences according to general characteristics, t-test was used. Results: Awareness scores about the importance of muscle health and exercises were 8.3 and 13.0, respectively. Attitude score and knowledge score were 12.4 and 15.0, respectively. There were differences in attitude toward muscle health according to gender, age, physical activity, and diet habits. However, there was no difference in attitude toward muscle health according to educational level, smoking, drinking, or sleeping. Conclusion: It is necessary to seek a strategy to improve awareness and attitude toward muscle health based on knowledge, not merely to raise knowledge level about muscle health. Since nurses play a central role in health promotion and disease prevention, they should also play an important role in strategic development and application of intervention.

• Journal of Korean Biological Nursing Science
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• v.24 no.1
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• pp.26-35
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• 2022

Purpose: The purpose of this study was to investigate the influencing factors on health-related quality of life according to body mass index (BMI) in middle age people. Methods: Using the 2017 Korea Health Panel Data, a total of 4,711 subjects age 40-64 were selected and classified into four groups based on BMI levels (underweight, normal weight, overweight, obese). Influencing factors according to BMI in middle age people was analyzed using multiple regression. Results: Activity limited was the most influential factor for health-related quality of life in middle age people. Walking and stress were factors influencing health-related quality of life in all weight groups. In the overweight and obese group, suicidal impulse was a significant influencing factor on health-related quality of life. Conclusion: To improve the health-related quality of life of middle age people, effective walking and stress management for maintaining and managing health, as well as managing suicide risk in the overweight group and obese group are required.