• Title/Summary/Keyword: biological health

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A Phenomenological Study on Mother-Infant Interacting Behavior Patterns Related to Newborn Infant Feeding in Korea (한국인 영아초기 수유시 모아상호작용 행동형태에 관한 현상학적 연구)

  • 한경자
    • Journal of Korean Academy of Nursing
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    • v.21 no.1
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    • pp.89-116
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    • 1991
  • The purpose of this study was to describe mother -infant interacting behavior patterns related to newborn infant feeding and to explore the mother's cultural belief about their infant. The data collection was conducted by observation and interview. Twenty-five mothers and their newborn infants who were normally delivered and were also planned to breast feed were comprised as the subjects of this study. All subjects were interviewed and observed individuaily at 1 to 5 days after the delivery at the hospital, mid -wife's clinic, Maternal Child Health Center and their home throughout the country from remote area to big city, The observation data were recorded with symbolic letter on a recording sheet newly developed as a result of preliminary study. The interview data were taperecorded and then recorded in narrative form. Mother - infant interaction behaviors in early feeding period were analyzed based on 19 analytic sub-categories and their composing elements. Unit of analysis were mother, infant and mother -infant dyad. 8 analytic categories draw from the data. Each were preparation, instrument, interaction inducing, evaluation referred to mother's behavior, preparation, instrument, interaction inducing referred to infant's behavior and synchronic behaviors referred to mother - infant dyad. Frequencies of behavior items based on the categories were converted to percent. The result showed that in mother's preparation behavior, the breast condition of Korean mother can be an affecting factor for mother - infant interaction during feeding, and vocalization behavior was observed most frequently in interaction inducing behavior while the least frequent behavior observed was contacting. Subcultural characteristics of mother - infant interaction behaviors were analyzed for their relationships between groups of mothers who have lived in remote area vs urban area, and who were multipara vs primipara. Using a chi -square test, there were statistically significant relationships in the activity of psychological readiness in preparation behavior and the movement of extremities for the position of instrumental behavior in both groups. However, interaction inducing behaviors were not related with statistical significance in any set of groups. Accomplishment of marriage, bonding and emotional mediation of family members were the categories related to mother's cultural belief about the infant in aspect of functional values. Infant at birth is considered little more than a biological organism without social capabilities. Although the newborn infant is still be attached to his mother, he makes his mother extend her territoriality. The mother's interacting behavior toward her infant based on those beliefs appeared task oriented, separative behavioral series. On the other hand, it was seen that infant reacted independently to his mother's behavior by the in-nate perceptual abilities. Those independent behavioral series of mother and infant on the feeding situation were synchronized at any moment. Nurses are In a unique position to teach mothers about their infant's capabilities and help reducing some of uncertainty about infant's behaviors. Study results indicated that the informations infant's social capabilities and breast feeding should be given to the mothers. The results of this study have several implications for nursing. First, the study results will be used as fundamental resources for the development of the assessment tool about the early mother - infant interaction. Second, the results could be a relevant information in the fied. I of maternal child nursing education as real and useful data. Third, the behavioral patterns of early mother - infant interaction which were classified based on the qualitative analysis could be used for nursing theory development as very fundamental data.

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Sex Steroids Regulate Expression of Genes Containing Long Interspersed Elements-1s in Breast Cancer Cells

  • Chaiwongwatanakul, Saichon;Yanatatsaneejit, Pattamawadee;Tongsima, Sissades;Mutirangura, Apiwat;Boonyaratanakornkit, Viroj
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.8
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    • pp.4003-4007
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    • 2016
  • Long interspersed elements-1s (LINE-1s) are dispersed all over the human genome. There is evidence that hypomethylation of LINE-1s and levels of sex steroids regulate gene expression leading to cancer development. Here, we compared mRNA levels of genes containing an intragenic LINE-1 in breast cancer cells treated with various sex steroids from Gene Expression Omnibus (GEO), with the gene expression database using chi-square analysis (http://www.ncbi.nlm.nih.gov/geo). We evaluated whether sex steroids influence expression of genes containing an intragenic LINE-1. Three sex steroids at various concentrations, 1 and 10 nM estradiol (E2), 10 nM progesterone (PG) and 10 nM androgen (AN), were assessed. In breast cancer cells treated with 1 or 10 nM E2, a significant percentage of genes containing an intragenic LINE-1 were down-regulated. A highly significant percentage of E2-regulated genes containing an intragenic LINE-1 was down-regulated in cells treated with 1 nM E2 for 3 hours (p<3.70E-25; OR=1.91; 95% CI=2.16-1.69). Similarly, high percentages of PG or AN-regulated genes containing an intragenic LINE-1 wwere also down-regulated in cells treated with 10 nM PG or 10 nM AN for 16 hr (p=9.53E-06; OR=1.65; 95% CI=2.06-1.32 and p=3.81E-14; OR=2.01; 95% CI=2.42-1.67). Interestingly, a significant percentage of AN-regulated genes containing an intragenic LINE-1 was up-regulated in cells treated with 10 nM AN for 16 hr (p=4.03E-02; OR=1.40; 95% CI=1.95-1.01). These findings suggest that intragenic LINE-1s may play roles in sex steroid mediated gene expression in breast cancer cells, which could have significant implications for the development and progression of sex steroid-dependent cancers.

Trend of the Changes in the Level of Blood Lead, Urinary Arsenic and Urinary Cadmium of children in Ulsan ; 3-year Follow-up Study (울산지역 어린이들의 혈중 연, 요중 비소 및 카드뮴 농도 변화추이 - 3년 추적조사)

  • Yoo, Cheol-In;Lee, Ji-Ho;Lee, Hun;Kim, Yang-Ho;Lee, Choong-Ryeol
    • Journal of Preventive Medicine and Public Health
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    • v.34 no.2
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    • pp.166-174
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    • 2001
  • Objectives : To obtain basic data on blood lead level and urinary level of arsenic and cadmium of children living near a petrochemical estate and a suburban area in Ulsan, Korea and to observe the trend of the changes. In the level of these metals in these children. Methods : The study subjects comprised 626 children living near a petrochemical estate and 299 children living in a suburban area of Ulsan. We analyzed the level of lead, arsenic and cadmium using atomic absorption spectrometer. Results : The mean levels of blood lead in children living near the petrochemical estate were $5.25{\mu}g/dl,\;5.24{\mu}g/dl,\;and\;7.24{\mu}g/dl$, and in the years 1997, 1999, and 2000, respectively, whereas those of children living in the suburban area were $3.81{\mu}g/dl,\;4.75{\mu}g/dl,\;and\;7.19{\mu}g/dl$ respectively. The mean levels of urinary arsenic in children living near the petrochemical estate were $4.57{\mu}g/g$ creatinine, $4.78{\mu}g/g$ creatinine, and $6.02{\mu}g/g$ creatinine in the year 1997, 1999, and 2000 respectively, whereas those of children living in suburban area were $2.35{\mu}g/g$ creatinine, $4.75{\mu}g/g$ creatinine, and $7.07{\mu}g/g$ creatinine, respectively. The mean levels of urinary cadmium in children living near the petrochemical estate were $1.15{\mu}g/g$ creatinine, $1.05{\mu}g/g$ creatinine, and $1.71{\mu}g/g$ creatinine in the year 1997, 1999, and 2000, respectively, whereas those of the children living in the suburban area were $0.74{\mu}g/g$ creatinine, $1.29{\mu}g/g$ creatinine, and $1.48{\mu}g/g$ creatinine, respectively. There were increasing trends in the level of blood lead, urinary arsenic and cadmium of children in Ulsan, and the differences in the level of these metals were disappearing between the children living in other areas year by year. Conclusions : These results suggest that the amount of exposure to lead, arsenic, and cadmium is increasing from year to year, and there is a need for periodic biological and atmospheric monitoring of these metals in Ulsan.

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Effects of Dissolved Microcystin-LR on the Different Phytoplankton Communities in a Microcosm Scale (용존성 독소 microcystin-LR이 식물플랑크톤 군집에 미치는 영향)

  • Suh, Mi-Yeon;Han, Myung-Soo;Kim, Baik-Ho
    • Korean Journal of Ecology and Environment
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    • v.38 no.3 s.113
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    • pp.313-321
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    • 2005
  • Based on the result that biological control agent (BCA) increased the dissolved microcystin-LR in a field experiment to control the cyanobacterial bloom (Kim etal., 2005), a laboratory experiment was used to evaluate the effects of dissolved microcystin-LR (MCLR) with different concentrations on abundance, dominance, diversity of phytoplankton community, concentration of chlorophyll a and microcystin concentration in replicated microcosms. The treatments in this laboratory experiment comprised different concentrations of T1 (natural MCLR concentration), T10 (ten times to natural MCLR concentration), and T100 (one hundred times to natural MCLR concentration). MCLR treatment of exclusively Stephanodiscus hantzschii-dominated community in Chonho bridge hardly changed in algal species, but abundance. In Kildong pond, Aulacoseira and Dinobryonrich community was replaced by green algae Scenedesmus-rich community especially in T100 experiment. However, in Yangsoori-Ryukgakji Pond having the highest concentration of initial MCLR, Microcystis aeruginosa was decreased in abundance. Therefore, the treatment of BCA to control M. aeruginosa severely changed the Phytoplankton community in term of algal species, abundance (chlorophyll a) and dissolved microcystin-LR via a high release of MCLR.

Seasonal Dynamics of Fish Fauna and Compositions in the Gap Stream Along With Conventional Water Quality

  • Lee, Jae-Hoon;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.40 no.4
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    • pp.503-510
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    • 2007
  • The purposes of the study were to analyze the seasonal effects on the fish fauna and compositions including trophic guilds and tolerance guilds. For the study, we collected fish samples twice in June as premonsoon period and early September 2007 as monsoon periods in five sampling sites of the Gap Stream, and then biological oxygen demand (BOD), nutrients (TN, TP) and suspended solids (SS) were compared with the guild data along the gradient of upstream-to-downstream. Chemical water quality, based on BOD, TP, and TN degraded gradually from the upstream to downstream reach and there were about 3 fold difference between S1 and S5. Water quality was worse in the premonsoon than the monsoon, and the heavy monsoon resulted in a dilution of the polluted river by rain water, especially, in the downstream reach. Total number of fish species, based on the catch per unit effort (CPUE), showed a distinct difference between the two seasons; 30 species were sampled in premonsoon, but 23 species were sampled in the monsoon, indicating a seasonal difference in the fish fauna. Tolerant species dominated the fish community (48.3%) in the stream, and the proportions prior to physical disturbance by the monsoon rain were evidently greater in the downstream reach than the upstream. This reflected the characteristics of urban stream polluted by nutrient enrichment as shown in the BOD and TP values. Sensitive species in the premonsoon decreased from the gradient of upstream-to-downstream reach. Such seasonal modifications in the trophic and tolerance guilds were evident. In the analysis of trophic guild and habitat guild, during the premonsoon the proportion of insectivore and riffle-benthic species were largely greater in the upstream reach than the downstream, whereas the proportions were opposite along the gradient of the stream in monsoon. Thus, the patterns of chemical water quality along the longitudinal gradients reflected the premonsoon conditions of insectivores and tolerant species, indicating that summer monsoon data of fish may not match with water quality due to large physical disturbance by flow regime. Seasonal monsoon in this region as well as the chemical pollution may act as a key role influencing the fish compositions of trophic and tolerance guilds and fauna. The data collected during the premonsoon rather than the monsoon, thus, may be better predictor for a diagnosis of stream health conditions.

Recovery of Fish Community and Water Quality in Streams Where Fish Kills have Occurred (어류폐사의 발생 이후 하천에서 수질의 변화 및 어류상 회복)

  • Lee, Jae-Yong;Lee, Kwang-Yeol;Lee, Saeromi;Choi, Jaeseok;Lee, Seok-Jong;Jung, Sungmin;Jung, Myoung-Sook;Kim, Bomchul
    • Korean Journal of Ecology and Environment
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    • v.46 no.2
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    • pp.154-165
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    • 2013
  • The species composition of the fish community was studied in four streams where fish kills have previously occurred in recent years; Worun Stream, Seo Stream, Jangnam Stream, and the Anyang Stream. The number of fish species varied from 22 to 86% compared to the number of fish species pre-fish kill. The recovery of fish populations in the streams seems to be determined by water quality and the presence of artificial structures in the streams. The Jangnam Stream, where alkaline pH caused a fish kill, showed a high recovery of fish species due to improvements in water quality. Anyang Stream had a low number of species, possibly because dissolved oxygen concentrations were intermittently low. Artificial structures in streams had a negative impact the recovery of fish species, particularly for benthic fish species. It appears that fish populations can recover rapidly when water quality improves or the movement of fish community is unimpeded. However, water quality and artificial structures in many streams in Korea still present adverse conditions for fish survival, deterring the recovery of fish populations. To conserve fish populations in streams, habitat all owing unimpeded movement for fish and controls on pollutant inputs are needed.

Korean Epithelial Ovarian Cancer Study (Ko-EVE): Protocols and Interim Report

  • Ma, Seung Hyun;Kim, Byoung-Gie;Choi, Ji-Yeob;Kim, Tae-Joong;Kim, Yong-Man;Kim, Jae Weon;Kang, Sokbom;Kang, Daehee;Yoo, Keun-Young;Park, Sue K.
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.8
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    • pp.3731-3740
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    • 2012
  • Background: There have been few studies of Asian ovarian cancer and benign tumors. The primary aim of this paper was to report the protocol of the Ko-EVE study to examine epidemiological and molecular factors for ovarian cancer and benign neoplasms and to ascertain the major risk factors for ovarian cancer control in Korea. Methods: This case-control study covers incident epithelial ovarian cancers and benign neoplasms, four major centers participating in enrolling incident cases and 3 hospitals enrolling healthy controls among health examinees. Standardized questionnaires were administered by trained interviewers, including sections on socio-demographics characteristics, past medical history, medication usage, family history, lifetime consumption of alcohol and tobacco, diet, physical activity, and reproductive factors for women. Various biological specimens were collected in the biorepository according to the standardized protocol. Annual follow-up for cancer cases and follow-up at the 1st year for benign tumor cases are performing to evaluate treatment effect and progression. Passive follow to see long-term survival will be conducting using record linkage with national data. Results: The total number recruited in 2010-2011 was 246 epithelial ovarian cancer cases, 362 benign epithelial tumors and 345 controls. We are planning to collect subjects for at least 1,500 sets of ovarian cancer, 2,000 benign tumors and 1,500 controls till 2018. Conclusions: The Ko-EVE will provide unique and important data to probe the etiology and natural history of Korean epithelial ovarian cancer. It will be continued by genomic and proteomic epidemiological analyses and future intervention studies for the prevention of ovarian cancer among Koreans.

Expression of Transcription Factor FOXC2 in Cervical Cancer and Effects of Silencing on Cervical Cancer Cell Proliferation

  • Zheng, Chun-Hua;Quan, Yuan;Li, Yi-Yang;Deng, Wei-Guo;Shao, Wen-Jing;Fu, Yan
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.4
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    • pp.1589-1595
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    • 2014
  • Objective: Forkhead box C2 (FOXC2) is a member of the winged helix/forkhead box (Fox) family of transcription factors. It has been suggested to regulate tumor vasculature, growth, invasion and metastasis, although it has not been studied in cervical cancer. Here, we analyzed FOXC2 expression in cervical tissues corresponding to different stages of cervical cancer development and examined its correlation with clinicopathological characteristics. In addition, we examined the effects of targeting FOXC2 on the biological behavior of human cervical cancer cells. Methods: The expression of FOXC2 in normal human cervix, CIN I-III and cervical cancer was examined by immunohistochemistry and compared among the three groups and between cervical cancers with different pathological subtypes. Endogenous expression of FOXC2 was transiently knocked down in human Hela and SiHa cervical cells by siRNA, and cell viability and migration were examined by scratch and CCK8 assays, respectively. Results: In normal cervical tissue the frequency of positive staining was 25% (10/40 cases), with a staining intensity (PI) of $0.297{\pm}0.520$, in CIN was 65% (26/40cases), with a PI of $3.00{\pm}3.29$, and in cancer was 91.8% (68/74 cases), with a PI of $5.568 {\pm}3.449$. The frequency was 100% in adenocarcinoma (5/5 cases) and 91.3% in SCCs (63/69 cases). The FOXC2 positive expression rate was 88.5% in patients with cervical SCC stage I and 100% in stage II, showing significant differences compared with normal cervix and CIN. With age, pathologic differentiation degree and tumor size, FOXC2 expression showed no significant variation. On transient transfection of Hela and SiHa cells, FOXC2-siRNA inhibition rates were 76.2% and 75.7%; CCK8 results showed reduced proliferation and relative migration (in Hela cells from $64.5{\pm}3.16$ to $49.5{\pm}9.24$ and in SiHa cells from $60.1{\pm}3.05$ to $44.3{\pm}3.98$) (P < 0.05). Conclusion: FOXC2 gene expression increases with malignancy, especially with blood vessel hyperplasia and invasion degree. Targeted silencing was associated with reduced cell proliferation as well as invasion potential.

Rhizospheric fungi of Panax notoginseng: diversity and antagonism to host phytopathogens

  • Miao, Cui-Ping;Mi, Qi-Li;Qiao, Xin-Guo;Zheng, You-Kun;Chen, You-Wei;Xu, Li-Hua;Guan, Hui-Lin;Zhao, Li-Xing
    • Journal of Ginseng Research
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    • v.40 no.2
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    • pp.127-134
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    • 2016
  • Background: Rhizospheric fungi play an essential role in the plantesoil ecosystem, affecting plant growth and health. In this study, we evaluated the fungal diversity in the rhizosphere soil of 2-yr-old healthy Panax notoginseng cultivated in Wenshan, China. Methods: Culture-independent Illumina MiSeq and culture-dependent techniques, combining molecular and morphological characteristics, were used to analyze the rhizospheric fungal diversity. A diffusion test was used to challenge the phytopathogens of P. notoginseng. Results: A total of 16,130 paired-end reads of the nuclear ribosomal internal transcribed spacer 2 were generated and clustered into 860 operational taxonomic units at 97% sequence similarity. All the operational taxonomic units were assigned to five phyla and 79 genera. Zygomycota (46.2%) and Ascomycota (37.8%) were the dominant taxa; Mortierella and unclassified Mortierellales accounted for a large proportion (44.9%) at genus level. The relative abundance of Fusarium and Phoma sequenceswas high, accounting for 12.9% and 5.5%, respectively. In total,113 fungal isolates were isolated from rhizosphere soil. They were assigned to five classes, eight orders (except for an Incertae sedis), 26 genera, and 43 species based on morphological characteristics and phylogenetic analysis of the internal transcribed spacer. Fusarium was the most isolated genus with six species (24 isolates, 21.2%). The abundance of Phoma was also relatively high (8.0%). Thirteen isolates displayed antimicrobial activity against at least one test fungus. Conclusion: Our results suggest that diverse fungi including potential pathogenic ones exist in the rhizosphere soil of 2-yr-old P. notoginseng and that antagonistic isolates may be useful for biological control of pathogens.

Antioxidant Activity of Lactic Acid Bacteria Isolated from Korean Traditional Food Kimchi (한국전통식품 김치로부터 분리한 유산균주의 항산화 활성)

  • Kim, Da-Young;Kim, Hong Seok;Yoo, Jung Sik;Cho, Yoon Ah;Kim, Cheol-Hyun
    • Journal of Dairy Science and Biotechnology
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    • v.38 no.2
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    • pp.89-98
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    • 2020
  • The purpose of this study was to investigate the probiotic properties of lactic acid bacteria (LAB) isolated from a Korean traditional food kimchi. Gram staining was performed by Macrogen (Macrogen, Inc.) for identification of the LAB. Five strains of LAB were identified, including DKGF9 (Lactobacillus plantarum), DKGF1 (L. paracasei ), DKGF8 (L. casei ), DK207 (L. casei ), and DK211 (L. casei ). The biological activities of the isolated strains were assessed. The results showed that heat resistance of the strains was similar to or higher than the commercial strain L. acidophilus LA-5. Indirect testing of the ability of the strains to attach to the mucin layer revealed that DKGF9, DKGF1, and DKGF8 have high binding affinities for the mucous layer. All strains showed antimicrobial activity similar to or higher than the commercial strain LA-5. In proteolysis experiments, the diameters of proteolysis zones of the five strains increased in the period of 24-72 h, with DKGF1 exhibiting the largest zone diameter. Three strains were selected based on their antioxidant activities. Among the five isolated strains, L. paracasei DKGF1 showed potential probiotic activity, and thus, it may be useful for the development of health-promoting products.