• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.20 no.1
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• pp.43-52
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• 2015

The growth and population dynamics of eelgrass (Zostera marina) due to changes in sediment composition were examined in the lower intertidal zone of the Seomjin Estuary, Korea. We surveyed environmental factors such as water temperature, underwater irradiance, main types and organic content of sediment, tidal exposure, and nutrient concentrations in the water column and sediment pore water, in relation to the shoot density, biomass, morphological characteristics, and growth of Z. marina inhabiting lower intertidal zones. The survey was conducted monthly from May to December of 2004 and 2009. The water temperature showed obvious seasonal trends in both study years. Underwater irradiance was significantly higher in 2009 than in 2004. Tidal exposure was not significantly different between 2004 and 2009. The sediment was muddy-sand in 2004 but became sandy and with a significantly lower organic content in 2009. Water column $NH_4{^+}$ concentrations were significantly higher in 2004 than in 2009. Sediment pore water $NO_3{^-}+NO_2{^-}$ concentrations were significantly higher in 2009 than in 2004. Other nutrient concentrations did not differ significantly between 2004 and 2009. Morphological characteristics, including eelgrass length and leaf width were significantly lower in 2009 than in 2004. Eelgrass shoot height, leaf length, and sheath length showed typical seasonal patterns, increasing in early summer and decreasing in autumn, in both years. Vegetative shoot density was not significantly different between 2004 and 2009, while the biomass of individual plant parts and the total biomass were significantly lower in 2009. Eelgrass leaf productivity did not differ between years, but leaf turnover time was significantly shorter in 2009 than in 2004. Eelgrass downsizing and decreased turnover time in 2009 compared to 2004 indicate more effective adaptations to the stress of long-term changes in sediment composition. Overall, results suggest that changes in sediment composition can be a limiting factor for seagrass growth in the intertidal zone.

• Journal of Life Science
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• v.28 no.11
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• pp.1339-1346
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• 2018

In Korea, edible mushrooms are produced largely on commercial artificial media, so the annual production of spent mushroom substrate (SMS), as a by-product of the mushroom industry, is estimated at over 200 million tons. This SMS is assumed to contain abundant fungal mycelia and pre-fruiting bodies, as well as various nutritive and bioactive compounds that are presently discarded. This study examined the physico-chemical, nutritional, and enzymatic characteristics of uninoculated sterilized medium (USM) and SMS of shiitake mushrooms with the aim of developing a high-value added product from SMS. The contents of crude protein, crude lipid, and ash were higher after the third SMS harvest ($SMS-A-3^{rd}$) than in USM or $SMS-A-1^{st}$. The contents of Ca, Mg, and P in $SMS-A-3^{rd}$ were 2.95, 2.35, and 2.1-fold higher compared than in USM. No As or Cd was detected in USM or SMS. The pH, Brix, and acidity were 4.6, 20.0, and 1.4, respectively in $SMS-A-3^{rd}$, but 5.6, 6.0, and 0.0, respectively, in USM. These results suggest a highly active production of soluble components and organic acids in $SMS-A-3^{rd}$. The distinct color differences noted for USM, $SMS-A-1^{st}$, and $SMS-A-3^{rd}$ could be used as a mycelial growth indicator. Enzyme activity assays using the APIZYM system showed that SMS is a potent source of hydrolysis-related enzymes, especially esterase (C4) and ${\beta}$-glucuronidase. Our results suggested that the SMS of shiitake has a high potential for use in environmental, agricultural, and stock-breeding industries, for example, as active ingredients for sewage treatment, waste-polymer degradation, and feed additives.

• Korean Journal of Ecology and Environment
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• v.56 no.1
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• pp.57-69
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• 2023

The objectives of this study were to analyze the fish community structures and distribution characteristics of fisheries resources in the Osip Stream and Wangpi Stream, fishery resources protection areas. For the study, we conducted fish samplings four times in the two streams from April to October 2019. In Osip Stream and Wangpi Stream, we sampled 31 species and 29 species and the dominant species were Zacco platypus (28.6%) and Squalidus multimaculatus(41.7%), respectively. According to the life type of fish species, primary freshwater species were collected 18 species(58.1%) and 21 species(72.4%), and migration fishes were sampled 4 species (12.9%) and 6 species (20.7%) in Osip Stream and Wangpi Stream, respectively. Fisheries resources species showed high relative abundance in June, and the fish biomass was highest in October. As a results of comparing the previous studies with this study, both rivers showed high biodiversity and were found to maintain stable populations of fishes. According to analysis of tolerance guilds, the proportion of tolerant species, based on the number of individuals, was composed of 14.4% and 1.1% in Osip Stream and Wangpi Stream, respectively. This very low ratio of tolerant species confirmed that both streams have very good physiochemical environment conditions. From the above results, it was judged that the fishery resource protection areas of Osip Stream and Wangpi Stream have high ecological function and preservation value. For sustainable use and management of fishery resource protection areas of Osip Stream and Wangpi Stream, it is suggested that biological disturbance management, fish discharge projects considering environmental capacity, efforts to improve the habitat environment, and establishing a fishery resource protection areas management system were necessary as considerations.

• Journal of Wetlands Research
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• v.18 no.4
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• pp.474-480
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• 2016

In this study, formation background of biodiversity and its changes in the process of geologic history, and effects of climate change on biodiversity and human were discussed and the alternatives to reduce the effects of climate change were suggested. Biodiversity is 'the variety of life' and refers collectively to variation at all levels of biological organization. That is, biodiversity encompasses the genes, species and ecosystems and their interactions. It provides the basis for ecosystems and the services on which all people fundamentally depend. Nevertheless, today, biodiversity is increasingly threatened, usually as the result of human activity. Diverse organisms on earth, which are estimated as 10 to 30 million species, are the result of adaptation and evolution to various environments through long history of four billion years since the birth of life. Countlessly many organisms composing biodiversity have specific characteristics, respectively and are interrelated with each other through diverse relationship. Environment of the earth, on which we live, has also created for long years through extensive relationship and interaction of those organisms. We mankind also live through interrelationship with the other organisms as an organism. The man cannot lives without the other organisms around him. Even though so, human beings accelerate mean extinction rate about 1,000 times compared with that of the past for recent several years. We have to conserve biodiversity for plentiful life of our future generation and are responsible for sustainable use of biodiversity. Korea has achieved faster economic growth than any other countries in the world. On the other hand, Korea had hold originally rich biodiversity as it is not only a peninsula country stretched lengthily from north to south but also three sides are surrounded by sea. But they disappeared increasingly in the process of fast economic growth. Korean people have created specific Korean culture by coexistence with nature through a long history of agriculture, forestry, and fishery. But in recent years, the relationship between Korean and nature became far in the processes of introduction of western culture and development of science and technology and specific natural feature born from harmonious combination between nature and culture disappears more and more. Population of Korea is expected to be reduced as contrasted with world population growing continuously. At this time, we need to restore biodiversity damaged in the processes of rapid population growth and economic development in concert with recovery of natural ecosystem due to population decrease. There were grand extinction events of five times since the birth of life on the earth. Modern extinction is very rapid and human activity is major causal factor. In these respects, it is distinguished from the past one. Climate change is real. Biodiversity is very vulnerable to climate change. If organisms did not find a survival method such as 'adaptation through evolution', 'movement to the other place where they can exist', and so on in the changed environment, they would extinct. In this respect, if climate change is continued, biodiversity should be damaged greatly. Furthermore, climate change would also influence on human life and socio-economic environment through change of biodiversity. Therefore, we need to grasp the effects that climate change influences on biodiversity more actively and further to prepare the alternatives to reduce the damage. Change of phenology, change of distribution range including vegetation shift, disharmony of interaction among organisms, reduction of reproduction and growth rates due to odd food chain, degradation of coral reef, and so on are emerged as the effects of climate change on biodiversity. Expansion of infectious disease, reduction of food production, change of cultivation range of crops, change of fishing ground and time, and so on appear as the effects on human. To solve climate change problem, first of all, we need to mitigate climate change by reducing discharge of warming gases. But even though we now stop discharge of warming gases, climate change is expected to be continued for the time being. In this respect, preparing adaptive strategy of climate change can be more realistic. Continuous monitoring to observe the effects of climate change on biodiversity and establishment of monitoring system have to be preceded over all others. Insurance of diverse ecological spaces where biodiversity can establish, assisted migration, and establishment of horizontal network from south to north and vertical one from lowland to upland ecological networks could be recommended as the alternatives to aid adaptation of biodiversity to the changing climate.

• Korean journal of applied entomology
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• v.15 no.1 s.26
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• pp.7-16
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• 1976

In order to increase utility efficiency of red wood ants, Formica rufa truncicola var. yessonesis Forel as a resource of natural enemy of pine caterpillar, Dendrolimus spectabilis Butler, by finding out ecological and environmental factors in the habitat of red wood ants, the nest distribution and its density in habitat, plant distribution and density, stand-density of red pine, nest building and fixing plants, relative humidity of surface soil, physical and chemical natures of soil, and breeding rate were examined. The obtained results are summarized as follows: 1. The nest of red wood ants was densely distributed, in the lower-and middle top of mountain but no nest was found in the top. 2. The economical distribution of nest of habitat was estimated as $2.85/m^2$ and the lowest density as $1.93/m^2$ and these estimation lead us to confirm that pine caterpillar could be controlled. 3. The ecological characteristics of habitat seemed to be represented as higher stand-density of red pine of 10-20 years of age with large areas of eroded land under trees. The major grasses prevailing in this area were Andropogon brevifolius. Arundinella hirta, Miscanthus purpurasens, Eulia speciosa, Themeda japonica, Cymbopogon goeringii, and Eccoilpus cotulifer 4. Red wood ants seemed to build the nest by using red pine, Arundinella hirta, Miscanthus purpurascens, Themeda japonica or Cymbopogon goeringii as a fixing plant. 5. The limited point of humidity percent in habitat of red wood ants was estimated as $76\%$ during the acting period of May to September and as $72\%$ during pre-period of hibernation of October to November. 6. Soil analysis in habitating region showed higher concentration of organic matters and lower concentration of calcium and magnesium, and habitat was largely composed of silt and fine sand rather than coarse sand. 7. When the separated colony was transplanted to non-habitating red pine forest that seemed to have the similiar conditions as those of habitat, propagation and establishment of nest was possible.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.8 no.3
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• pp.285-294
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• 2003

To select an appropriate sampler for the environmental monitoring survey in coastal waters of Saemangeum, Jeollabuk-do, a macrobenthic sampling was conducted in April 2002. Employed samplers were dredge (type Charcot), a semi-quantitative sampler and Smith-McIntyre (SM) and van Veen grab (VV) as quantitative ones. One haul was tried for dredge and 3 replicates (0.1 ㎡${\times}$3) for SM and W at each of 11 stations. Comparisons of sediment volume in sampler bucket and of precision of biological parameters (i.e., density, biomass, species number and diversity index, H') were made between SM and VV. Sediment volume was significantly different (SM > VV) at p-value of 0.0050 (paired t-test) and, in average, 3 replicate samples of SM and VV satisfied a precision level of 0.2 by applying 4th root transformation. Patterns of observed and expected species numbers and H' were compared. Dredge-VV samples showed higher affinity than any other pair. Several dominant species in the area were underestimated in dredge samples (e.g., polychaete Heteromastus filiformis. Aricidea assimilis etc.). Quantifying the agreement pattern of multi-species responses was accomplished by estimating correlations between similarity matrices. Correlation between dredge and VV was slightly higher, but near-per-fect matches were found in general. Different ranks and composition among principal species lists were presumably linked to the effect of penetration depth that differs among samplers. Lower level of some species' abundance in VV samples (ca. 50％ compared with those of SM) was explained in this context. It seem appropriate to regard the effect as a probable cause of relatively higher correlations in dredge-VV, Overall bio-logica1 features indicated that a better choice could be SM in situations of requiring high data quality. The others work well, however, on observing and defining faunal characteristics and their capability cannot be questionted if we do not expect a first-order quality.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.257-267
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• 1997

Fruit bodies similar to the Phellinus sp. residing on the mulberry were collected at Yang-yang in Kang-won-do province and one strain of Phellinus sp. was isolated from the fruit bodies. For mass production of the isolated mycelia in a submerged culture, the culture conditions, medium composition, and the effect of various culture systems on the mycelial growth, were investigated. The morphological characteristics of the fruit body were as follows: covered with blackish to black and rough, lower surface with yellowish-brown to dull-brown and smooth, 5-7 cm thick and hard woody. Also, the pure cultured mycelia showed yellowish-brown color, capability of purplish-brown pigment production on the PDA plate media, no-formation of clamp-connection, much binding branch, and enzyme activities such as laccase, tyrosinase and peroxidase. Therefore, pure cultured strain was identified to be Phellinus sp. In the flask culture, the optimum culture conditions for the mycelial production were obtained after cultivation of 8 days at inoculum level of 5%(v/v), media volume of 70 mL, 150 rpm, initial pH 6, and temperature of $30^{\circ}C$. Optimum medium composition from the response surface analysis were determined to be glucose 12.12 g/L, sucrose 12.12 g/L, yeast extract 11.15 g/L, malt extract 11.15 g/L, $KH_2PO_4$ 0.855 g/L and $CaCl_2$ 0.855 g/L. The production of the mycelia after 4 and 8 days of cultivation was 1.95 and 9.89 g/L, respectively. The maximum specific growth rate and productivity were $0.020\;hr^{-1}$ and 1.25 g/L/day, respectively. Among the three different culture systems for the growth of mycelia, the maximum mycelial dry weight of 7.5 g/L was obtained after cultivation of 4 days in the air-lift fermentor under aeration rate of 2.5 vvm. The maximum specific growth rate and productivity were $0.033\;hr^{-1}$ and 1.9 g/L/day, respectively, which were about 1.7 and 4.2 times higher than those of flask culture.

• KSBB Journal
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• v.24 no.1
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• pp.1-8
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• 2009

In the past decade, we have observed rapid advances in the development of biochips in many fields including medical and environmental monitoring. Biochip experiments involve immobilizing a ligand on a solid substrate surface, and monitoring its interaction with an analyte in a sample solution. Metal nanoparticles can display extinction bands on their surfaces. These charge density oscillations are simply known as the localized surface plasmon resonance (LSPR). The high sensitivity of LSPR has been utilized to design biochips for the label-free detection of biomolecular interactions with various ligands. LSPR-based optical biochips and biosensors are easy to fabricate, and the apparatus cost for the evaluation of optical characteristics is lower than that for the conventional surface plasmon resonance apparatus. Furthermore, the operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in LSPR research and also describe the LSPR-based optical biosensor constructed with a core-shell dielectric nanoparticle biochip for its application to label-free biomolecular detections such as antigen-antibody interaction.

• Journal of Oral Medicine and Pain
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• v.33 no.3
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• pp.257-267
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• 2008

The mentophysical disease causes diseases in digestive, respiratory, circulating systems, including chronic pain, through combined reactions from different individual characteristics, mental stress and temperamental factors. The most common symptom related to orofacial area is pain and the contributive factors include biological, behavioral, environmental, social, emotional, recognitive factors. These factors affect the course of the symptom according to individual's character and human nature. In pain, sleep acts as a contributive factor, and pain could bring about sleep disturbance and vice versa. Deterioration of sleep quality would act as a factor that aggravates mental stress. Therefore, relatively accurate and simple mental examinations and sleep quality test should be carried out for the patients with symptoms related to orofacial area. This study evaluated the mental state in relation to the sleep quality which could affect orofacial pain. The number of poor sleeper was 18 in male subjects, and 1 in female subjects and PSQI global index was higher in male($6.11{\pm}2.38$) than female($4.67{\pm}2.18$). SCL-90-R index showed no sex difference. Poor sleeper showed significantly high value in SOM, O-C, I-S, ANX, PHOB, PSY, GSI, PST. When SCL-90-R T scores were compared according to sleep quality, higher the subjective sleep quality score, O-C and I-S showed significant increase. As sleep disturbances score increased, PAR, PSY, PST showed statistically significant increase. In comparison of SCL-90-R T score according to daytime dysfunction, statistically significant increase in DEP, ANX, HOS, PHOB, PAR, GSI was observed. Therefore, the quality of sleep and psychological status have a high correlation. This is likely to influence chronic pain in the orofacial field. As a result, clinicians treating orofacial pain should evaluate the sleep quality and psychological status of the patient. Further studies of larger sample sizes including various age, occupation, and pain groups are necessary in order to apply the results to clinical practice.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.212-219
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• 2004

To minimize the danger of soil erosion and settle habitats earlier, afforestation, which vegetates bare slopes, is selected as an environmental recovering technology. Large portions of these areas often are suffered by a bad germination and growth inhibition of sprayed seeds. Afforested materials collected in the normal and damaged sites were not any big difference in chemical characteristics and biological response to ryegrass. But background soil of the damaged site has very low pH (3.6) and high contents of iron and aluminum compared with them of the normal sites. Both germination and root growth of ryegrass were inhibited severely in the water extracts of damaged soils, but not in the water extracts of normal sites. Groundwater collected nearby the damaged sites was very strong acidic (pH 33) and exhibited a high value of electrical conductivity and high contents of iron and aluminum. In the ground water, germinated ryegrass was scarcely grown. In Al standard solution, the root growth of ryegrass was inhibited over 50% in 0.5 mM in pH 3.5-4.5 and in 1.4 mM in pH 5.5, which seems to be related to $Al^{3+}$ activity in solution. In the ferric Fe ($Fe^{3+}$) standard solution, ryegrass growth was inhibited over 50% in the concentration of 14-19 mM in root and 23-25 mM in shoot. This strong tolerance of ryegrass to $Fe^{3+}$ might be concerned with the very low activity of $Fe^{3+}$ at pH 3.5-5.5. In contrast, ryegrass responded very sensitively to ferrous Fe ion ($Fe^{2+}$), especially in root growth: $Fe^{2+}$ concentrations corresponding to 50% growth reduction were 0.3-0.4 mM at pH 3.5-5.5 in roots. This high growth inhibition should be related to the high ion activity of $Fe^{2+}$ irrespective of different pH conditions. In conclusion, low pH and high contents of $Fe^{2+}$ and aluminum seem to be caused by pyrite and be closely related to the growth inhibition of ryegrass seeded in afforested area.