• Title/Summary/Keyword: biochemical property

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The Isolation of Acetylcholinesterase Inhibitory Constituents from Lycoris radiata using On-line HPLC-biochemical Detection System

  • Yang, Hee-Jung;Yoon, Kee-Dong;Chin, Young-Won;Kim, Young-Choong;Kim, Jin-Woong
    • Natural Product Sciences
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    • v.16 no.4
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    • pp.228-232
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    • 2010
  • Bioactivity-guided fractionation using on-line HPLC biochemical detection system on $CHCl_3$-soluble fraction of Lycoris radiata led to the isolation of deoxylycorenine (1), O-demethylhomolycorine (2), galanthamine (3), lycoramine (4), mixture of $6{\alpha}$-and $6{\beta}$-haemanthidine (5), and lycorine (6), identified by spectroscopic data and physicochemical property. Among the isolated compounds, 1, 3 and 6 showed acetylcholinesterase inhibitiory activities with $IC_{50}$ values of 18.0, 12.0 and $16.6\;{\mu}M$, respectively, in in vitro colorimetric microplate assay.

Thermal Curing Property of Silicone Encapsulant Containing Quantum Dot Surrounded by Various Types of Ligands

  • Lee, Chae Sung;Kim, BeomJong;Jeon, Seongun;Han, Cheul Jong;Hong, Sung-Kyu
    • Bulletin of the Korean Chemical Society
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    • v.34 no.12
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    • pp.3787-3789
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    • 2013
  • In this study, the silicone thermal curing degree of the silicone-encapsulated quantum dot light emission diode was measured using the various types of chemical ligands around quantum dot. It was confirmed that the trioctyl phosphin oxide (TOPO) ligand around the quantum dot was responsible for dispersion of the quantum dot in silicone encapsulant and decline of the thermal curing degree of the silicone encapsulant. Also, it was confirmed that the thermal curing degree of silicone encapsulants containing the steric acid (SA) and the dodecanoic acid (DA) ligands were higher than the one of TOPO ligand.

Biosynthesis of Rhamnosylated Anthraquinones in Escherichia coli

  • Nguyen, Trang Thi Huyen;Shin, Hee Jeong;Pandey, Ramesh Prasad;Jung, Hye Jin;Liou, Kwangkyoung;Sohng, Jae Kyung
    • Journal of Microbiology and Biotechnology
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    • v.30 no.3
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    • pp.398-403
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    • 2020
  • Rhamnose is a naturally occurring deoxysugar present as a glycogenic component of plant and microbial natural products. A recombinant mutant Escherichia coli strain was developed by overexpressing genes involved in the TDP-ʟ-rhamnose biosynthesis pathway of different bacterial strains and Saccharothrix espanaensis rhamnosyl transferase to conjugate intrinsic cytosolic TDP-ʟ-rhamnose with anthraquinones supplemented exogenously. Among the five anthraquinones (alizarin, emodin, chrysazin, anthrarufin, and quinizarin) tested, quinizarin was biotransformed into a rhamoside derivative with the highest conversion ratio by whole cells of engineered E. coli. The quinizarin glycoside was identified by various chromatographic and spectroscopic analyses. The anti-proliferative property of the newly synthesized rhamnoside, quinizarin-4-O-α-ʟ-rhamnoside, was assayed in various cancer cells.

Effect of Storage and Marketing Condition on Biochemical Property Changes of Garlic (Allium sativum L.) (마늘(Allium sativum L.)의 생화학적 변화에 대한 저장 및 유통조건의 영향)

  • 최선태;장규섭
    • Food Science and Preservation
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    • v.5 no.2
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    • pp.111-117
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    • 1998
  • Biochemical property changes of garlic during various storages and marketing after storage were investigated. Content of enzymatic pyruvic acid increased by room and low temperature storage but decreased by CA and MA storage. Fructan contents decreased rapidly by low temperature storage, but restrained decrement by CA and MA storage. Free sugar increased during storage, but did slowly by room temperature storage. Green pigment development was observed when garlics stored for 90days at low temperature were processed into crushed form. This discoloration was small for garlics stored in CA and MA, and never occurred for room temperature stored garlics. When marketed after storage, content of enzymatic pyruvic acid decreased in garlic stored in room and low temperature storage, but increased in garlics which decreased during CA and MA storage. Fructan contents deceased but free sugar contents continuously increased with marketing period. Green pigment development decreased in crushed garlic after 30days at room temperature marketing, but increased in low temperature marketing with marketing time progress.

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Preparation of Waterborne Polyurethane Coating Solutions with Antistatic Property from Alkali Metal Salts (알카리 금속염으로부터 대전방지용 수분산 폴리우레탄 코팅용액 제조)

  • Hong, Min Gi;Kim, Byung Suk;Lee, Yong Woon;Song, Ki Chang
    • Korean Chemical Engineering Research
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    • v.50 no.3
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    • pp.427-434
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    • 2012
  • Waterborne polyurethane dispersions (PUD) were synthesized from poly (carbonate diol), isophrone diisocyanate and dimethylol propionic acid at different NCO/OH molar ratios. Subsequently, the PUD was mixed with different types of alkali metal salts ($LiClO_4$, $NaClO_4$, and $KClO_4$) to prepare antistatic waterborne polyurethane coating solutions. Effects of the types and amounts of alkali metal salts were investigated on the surface resistances of the resulting coating films. The surface resistances of coating films were decreased with increasing the amounts of alkali metal salts added in the PUD. The coating films prepared with the same amount of alkali metal salts showed increased ionic conductivity with the order of $LiClO_4$ > $NaClO_4$ > $KClO_4$. Also, the surface resistances of coating films were increased with increasing the molar ratios of NCO/OH in the PUD.

Isolation and Biochemical Properties of Pasteurella multocida from the pneumonic lungs of swine in Cheju (제주지방 돼지의 폐병변으로부터 Pasteurella multocida 분리 및 생화학적 특성)

  • 김옥녀;이두식;문호규;김우택;서문현;배종희;임윤규;조길재
    • Korean Journal of Veterinary Service
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    • v.18 no.2
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    • pp.124-132
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    • 1995
  • The present study was conducted to investigate the incidence of Pasteurella multocida infection in cheju swine from March 1994 to December 1994 isolated organisms were identified by the biochemical properties, cellulose serological type and antibiotic susceptibilities. Pasteurella multocida was isolated from the Lungs of 96 pigs with pneumonia(51 %) among 188 slaughtered pigs. The majority of p multocida isolates were identical to those of the standard strains. On the classification of the capsular type of the isolated p multocida it consist of the 88 isolates of type A(91.6%) 2 isolates of type D (2%) and un classified 6 types(6.2%). The majority of the 96 isolates of p multocida highly susceptible to the antibiotics including ampicillin(Am), cephalotin(Ce), erythromycin(Em), gentamycin(Gm), kanamycin(Km), lincomycin(Lm), neomycin(Nm), penicillin(Pc), streptomycin(Sm), solfametoxazol/trimethoprim(Sxt) and tetracycline(Te)

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Photocatalytic Properties of TiO2 According to Manufacturing Method (제조방법에 따른 TiO2의 광촉매 특성 분석)

  • Lee, Hong Joo;Park, Yu Gang;Lee, Seung Hwan;Park, Jung Hoon
    • Korean Chemical Engineering Research
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    • v.56 no.2
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    • pp.156-161
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    • 2018
  • $TiO_2$ photocatalyst powders were prepared by chlorination method and sol-gel method. Specific surface area and crystalline (i.e., anatase and rutile) of the catalyst varied depending on manufacture conditions and method. TTIP-sol photocatalyst had higher methylene blue (MB) decomposition characteristics than photocatalyst from chlorination method and TBOT-sol. MB removal efficiency from aqueous solution with TTIP-sol photocatalyst was over 90%. Experimental results showed that the $TiO_2$ photocatalyst with a single anatase phase and a large specific surface area had high decomposition characteristics of organic materials.