• 제목/요약/키워드: biochemical genetics

검색결과 130건 처리시간 0.025초

Overview of citrin deficiency and its incidence in Asian region

  • Kobayashi, Keiko;Iijima, Mikio;Ushikai, Miharu;Lu, Yao Bang;Sheng, Jian-Sheng;Tabata, Ayako;Ikeda, Sayaka;Li, Meng Xian;Saheki, Takeyori;Okano, Yoshiyuki;Hsiao, Kwang-Jen;Hwu, Wuh-Liang;Yang, Yanling;Lau, Yu-Lung;Tsui, Lap-Chee;Choeh, Kyuchul;Lee, Dong-Hwan
    • 대한유전성대사질환학회지
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    • 제6권1호
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    • pp.89-93
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    • 2006
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Characterization of the Replication Region of the Enterococcus faecalis Plasmid p703/5

  • Song, Joon-Seok;Park, Jin-Hwan;Kim, Chan-Wha;Kim, Young-Woo;Lim, Wang-Jin;Kim, Ick-Young;Chang, Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • 제9권1호
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    • pp.91-97
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    • 1999
  • In this work, a 1.9-kb region of enterococcal plasmid p703/5 was isolated and the nucleotide sequence analysis of the region was performed. One major open reading frame (ORF) was identified encoding a polypeptide of 28 kDa. Database comparisons suggested that the protein showed some homology with other bacterial RepA proteins. Upstream of the ORF, a potential dnaA box, AT-rich region and 22-bp tandemly repeated sequences (DNA iterons), a feature typical for many replication ori sites, were recognized. Deletion analysis using Exonuclease III and several restriction enzymes indicated that the three elements and the gene product from the ORF were essential for replication and that the minimum unit of DNA required for replication resided on the 1.2-kb AvaII subfragment. Thus, this gene product was referred to as RepA.

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Biochemical Performance and Quantitative Assessment of F1 Hybrid of Two Ecoraces of Tropical Tasar Silkworm Antheraea Mylitta Drury (Lepidoptera: Saturniidae)

  • Lokesh, Gangadharaiah;Tirkey, Sushma Rani;Srivastava, Ashok Kumar;Kar, Prasant Kumar;Sinha, Manoj Kumar
    • International Journal of Industrial Entomology and Biomaterials
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    • 제26권2호
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    • pp.67-73
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    • 2013
  • Antheraea mylitta Drury is basically a crossbreeding species, as such it seems to be potentially a good material for the exploitation of heterosis. In the present study F1 hybrid of wild ecorace Laria (L) and semi-domestic Daba (D) was raised and evaluated for various quantitative traits and biochemical parameters during larval stage. Improved fecundity ($+18{\pm}1.8%$ and higher egg hatching rate ($+10.96{\pm}1.3%$) was recorded in the F1hybrid ($L{\times}D$). Biochemical parameters studied in the hemolymph, midgut and fatbody of the larva showed significantly higher (P<0.05) total proteins and carbohydrate concentration besides digestive enzyme activity. Correspondingly SDS-PAGE revealed more number of protein bands in the hemolymph sample of F1s, ranging between 29 kDa to 66 kDa compared to parental lines. The present study demonstrates the positive heterosis effect in the F1 hybrid of Laria ${\times}$ Daba. Biochemical analysis indicates that, there is possibilities of exploitation of hybrids with specific parents targeted for desirable commercial traits (silk yield and fecundity). Moreover, most of these biochemical parameters can be used as markers to analyze the genetic improvement in the tasar silkworms.

Protective Effect of Astaxanthin Produced by Xanthophyllomyces dendrorhous Mutant on Indomethacin-Induced Gastric Mucosal Injury in Rats

  • Kim, Jeong-Hwan;Choi, Seok-Keun;Lim, Wang-Jin;Chang, Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • 제14권5호
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    • pp.996-1003
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    • 2004
  • Nonsteroidal anti-inflammatory drugs such as indomethacin induce severe gastric mucosal damage in humans and rodents. In the present study, the in vivo protective effect of astaxanthin on indomethacin-induced gastric lesions in rats was investigated. The test groups were injected with indomethacin (25 mg/kg) after the oral administration of astaxanthin (25 mg/kg) for 1, 2, and 3 days, while the control group was treated only with indomethacin. Thiobarbituric acid reactive substances in the gastric mucosa, as an index of lipid peroxidation, increased significantly after indomethacin administration and this increase was inhibited by oral administration of astaxanthin. In addition, pretreatment with astaxanthin resulted in a significant increase of the activities of superoxide dismutase (SOD), catalase, and glutathione peroxidase (GSH-px). Histologic examination clearly revealed acute gastric mucosal lesions induced by indomethacin in the stomach of the control group, but were not observed in that of the test group. These results indicate that astaxanthin activates SOD, catalase, and GSH-px, and removes the lipid peroxides and free radicals induced by indomethacin. It is evident that astaxanthin acts as a free radical quencher and antioxidant, and is an effective molecule in the remedy of gastric mucosal lesions.