• 미생물학회지
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• 제8권3호
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• pp.107-115
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• 1970

In order to brew Takju, Korean flour wine, it requires three necessary steps for specific brewage. One is primary brewing process and another main brewing process to sacharify and perform alcoholic fermentation. After previous two brewing passing, the mash of main brewing process mixed with 1 volume of water is commercial Takju. Three samples were obtained from three breweries and incubated at $28{\pm}1^{\circ}C$for the alcoholic fermentation. All the samples were analyzed for observation of the changes of various biochemical constituents which were contained in the mashes of two brewing processes and in Takju. The starch contents of the suspensions in the primary and main mashes, and in Takju were 28.08, 25.92, 3.83%, and decreased considerably within 36-48 hrs and thereafter slowly. The amounts of water soluble carbohydrates and reducing sugars in three steps had a tendency of decreasing within 48 hours. The initial numbers of yeasts per 1ml in the suspensions were $1.74{\times}10^8$, $1.65{\times}10^8$, $0.66{\times}10^8$, appeared the highest at 72 hours (12.66%) in main process, and in the case of Takju kept increasing untill 60 hours. The dextrin contents in the mashe of primary process through the long period were 0.34 - 0.68%, in the main one were 0.12 - 0.32%, concerning with the amylase activities which were stronger in the mash of the primary process than in that of the main. The contents of methanol and cellulose in the mashes of two processes were 3.40-0.68%, in the main one were 0.12-0.32%, concerning with the amylase activities which were stronger in the mash of the primary process than in that of the main. The contents of methanol and cellulose in the mashes of two processes were 3.40-5.98mg/ml, 0.43-0.48% during the fermentation. The consumption of crude protein the mashes of Takju revealed with time, on the contrary, the production of free amino acids and oligo-peptides were performed, depending upon the affection of proteinase. The amount of tannic acid in Takju was 0.0073-0.0098mg/ml and organic acids of these three groups increased with time and hydrogen ion concentration was 3.28-4.43.

• Asian-Australasian Journal of Animal Sciences
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• 제6권1호
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• pp.147-153
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• 1993

A total of 109 heads of horses and ponies from eight horseback riding clubs nearby Central Taiwan were investigated to evaluate the blood parameters and the biochemical compositions of serum for the documentation of clinical pathological diagnosis and exercise physiology. Blood samples were collected from the jugular vein of resting horses. The sex difference in the blood traits were compared both in horses and in ponies. Results shows that total plasma proteins (PP) and hematocrit (PCV) were found higher in male horses than in females (p < 0.05). The sexual effect also exertes a significant influence on the leucocyte (WBC) count, but not on the erythrocyte (RBC) concentration. According to the differential counts of leucocytes, the number of monocytes and lymphocytes was higher in the male pony than that of in male horse. A close relationship Between the erythrocyte sedimention rate (ESR) and the other blood parameters were found especially in PCV, RBC concentration, and plasma protein level. The average ESR observed at 60 minutes were $21.80{\pm}21.87mm$, $39.50{\pm}18.90mm$ and $43.73{\pm}17.89mm$ in stallions, geldings, and mares, respectively. Most of the biochemical components of horse serum detected were distributed in normal ranges, although some of the items show a great variation in such a large sample size.

• 생약학회지
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• 제44권2호
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• pp.138-148
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• 2013

The three biochemical constituents pointed out as the bioactive principles of anticancer activity of Korean mistletoes, Viscum album var. colorantum, epiphyting to different host trees, different region, and different growth environments were compared. The characterizing compounds were lectin, which was glycoprotein reported has anticancer activity, homoflavoyadorinin-B, which was flavonoid glycoside reported as an antioxidant and oleanolic acid, as an anticancerous triterpenoid. We established the analysis methods for three characterizing compounds and determined by HPLC spectrophotometer, respectively. The average content of the lectin in V. album was 1.75 mg/g of dried plants and those of homoflavoyadorinin-B and oleanolic acid were 3.51 and 5.37 mg/g, respectively. The variations of contents of the three characterizing compounds according to epiphytic trees and growth region were observed but not found statistical significance clearly. We came to the conclusion that the contents of bioactive constituents of V. album except homoflavoyadorinin-B are not affected by epiphyting trees or growth environments.

• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.69-74
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• 1994

The relationship of thyroid status and certain blood biochemical constituents with body weight gains (ADG) and age (13 to 96 weeks) was studied in Holstein Friesian ${\times}$ Hariana ($1/2F{\times}1/2H$) crossbred male calves by assessing their plasma triiodo thyronine ($T_3$), Thyroxine ($T_4$), sodium, potassium, total proteins and cholesterol level at two energy levels. Body weight gains (ADG) were higher during the 50 to 72 weeks of age and declined thereafter, the plasma $T_3$ conc. was aignificantly (p < 0.01) higher during this period compared to all other periods. There was no significant variation due to energy level. Overall mean plasma $T_3$ conc. was $1.19{\pm}0.12ng/ml$. Plasma $T_4$ conc. did not show any significant variation either between the different age periods or between the two energy levels. The mean plasma $T_4$ conc. was $37.34{\pm}1.32ng/ml$. The plasma sodium and potassium concentration did not vary significantly due to energy levels. But amongst the different age periods, sodium concentration was highest ($147.70{\pm}2.29mEq/L$) during 49-60 weeks of age and lowest ($134.70{\pm}1.78mEq/L$) during 13-24 weeks, where as for potassium concentration changes were nonsignificant. There was very little variation amongst other periods. Plasma protein level was higher at 100% energy level than at 75%. Amongst the different age period, it was significantly lower ($6.44{\pm}0.36$) during 13 to 24 weeks of age than at 37 to 48 weeks of age ($7.14{\pm}0.11$). Plasma cholesterol values were higher for 75% energy level than that of 100%. Between the periods it was highest during 61 to 72 weeks of age and the difference amongst the age period were highly significant.

• Environmental Engineering Research
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• 제16권3호
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• pp.121-129
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• 2011

The completely mixed flow and plug flow (CAP) water quality model was developed for streams with discontinuous flows, a condition that often occurs in low base flow streams with in-stream hydraulic structures, especially during dry seasons. To consider the distinct physical properties of each reach effectively, the CAP model stream network can include both plug flow (PF) segments and completely mixed flow (CMF) segments. Many existing water quality models are capable of simulating various constituents and their interactions in surface water bodies. More complicated models do not necessarily produce more accurate results because of problems in data availability and uncertainties. Due to the complicated and even random nature of environmental forcing functions, it is not possible to construct an ideal model for every situation. Therefore, at present, many governmental level water quality standards and decisions are still based on lumped constituents, such as the carbonaceous biochemical oxygen demand (CBOD), the total nitrogen (TN) or the total phosphorus (TP). In these cases, a model dedicated to predicting the target concentration based on available data may provide as equally accurate results as a general purpose model. The CAP model assumes that its water quality constituents are independent of each other and thus can be applied for any constituent in waters that follow first order reaction kinetics. The CAP model was applied to the Geum River in Korea and tested for CBOD, TN, and TP concentrations. A trial and error method was used for parameter calibration using the field data. The results agreed well with QUAL2EU model predictions.

• 한국환경농학회지
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• 제11권3호
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• pp.261-268
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• 1992

식물 세포의 미토콘드리아와 엽록체는 광 증감제로 작용할 가능성이 있는 여러 가지 색소들을 함유하고 있다. 이들 색소들은 대부분이 막에 결합되어있으며 청색광 영역에서 강한 흡수대를 가지므로 청색광하에서 노출된 이들 소기관에서는 활성산소가 발생되어 막의 구조적, 기능적 피해를 유발하는 요인이 된다. 활성산소의 광발생에 따른 막구조의 변화는 일차적으로 막지질 지방산성분의 산화적 파괴에 기인할 것이다. 본 연구에서는 식물이 고광도의 가시광선(특히 청색광선)하에서 일어나는 막지질 지방산의 산화적 파괴에 지방산 대신 상대적으로 둔감한 포화 지방산의 조성비를 높일 것이라고 가정하고, 이를 뒷받침할만한 실험결과를 얻었다. 즉, 광질이 서로 다른 가시광선 처리조건하에서 생장중인 식물 및 그 소기관들을 대상으로 막지질의 과산화, 지방산조성 및 불포화도, 막결합 단백질의 활성 등을 측정하고 이를 제시하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제3권1호
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• pp.89-92
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• 2001

The effect of topical application with 5,10 and 15 $\mu$g/ ml androstenedione and methyltesterone to the fifth stadium larvae of the si1kworm,B. mori on fat body glycogen, protein, tota1 lipids, phospholipids, neutral lipids and haemolymph trehalose and protein has been studied. The fat body glycogen increased significantly in all the treated groups except in the 15$\mu$g/ml androstenedione treated group. However the fat body glyco- gen increased significantly in 10 ${\mu}g$/ml methyltesterone treated group. The androstenedione treated groups showed increased haemolymph trehalose but the increase was not significant. However the haemolymph trehalose increased significantly in all the methyltestosterone treated groups. The fat body protein decreased in all the androstenedione and methyltesterone treated groups except 15 ${\mu}g$/ml methyltesterone where the decrease in fat body protein was significant. Whereas that of haemolymph protein significantly decreased in all the groups except in the group treated with 5 ${\mu}g$/ml androstenedione where the decrease was not significant. The total lipids, phospholipids and neutral lipids of the fat body decreased significantly in all the groups treated with androstenedione and methyltestosterone when compared with those of carrier control.

• International Journal of Industrial Entomology and Biomaterials
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• 제30권2호
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• pp.58-63
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• 2015

An experimental rearing of tropical tasar silkworm, Antheraea mylitta Drury was conducted to study silkworm-food plant-interaction and thereby to search for an alternate silkworm food plant. The silkworm-food-plant-interaction was studied with six different food plant species viz. Terminalia tomentosa, Terminalia arjuna, Terminalia belerica, Terminalia chebula of Combretaceae family and Lagerstroemia speciosa, Lagerstroemia parviflora of Lythraceae family. The rearing performance of silkworm on Lagerstroemia speciosa in terms of cocoons per DFL and silk ratio was found comparable with Terminalia tomentosa and Termonalia arjuna, the primary tasar silkworm food plant species. These three plant species also possessed better results in terms of physiological (leaf moisture content and net photosynthesis rate) and biochemical (Chlorophyll, protein, carbohydrate and crude fibre contents) characteristics to support silkworm rearing than Terminalia belerica, Terminalia chebula and Lagerstroemia parviflora. The correlation study between silkworm rearing performance and food plant's constituents indicates commercial perspective of Lagerstroemia speciosa as an alternate food plant for tasar silkworm rearing.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.151-155
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• 2005

Recently, systems biology has been increasingly applied to gain insights into the complexity of living organisms. Many inaccessible biological information and hidden evidences fur example flux distribution of the metabolites are simply revealed by investigation of artificial cell behaviors. Most bio-models are models of single cell organisms that cannot handle the multi-cellular organisms like plants. Herein, a structured and multi-cellular model of potato was developed to comprehend the root starch biosynthesis. On the basis of simplest plant cell biology, a potato structured model on the platform of Berkley Madonna was divided into three parts: photosynthetic (leaf), non-photosynthetic (tuber) and transportation (phloem) cells. The model of starch biosynthesis begins with the fixation of CO$_2$ from atmosphere to the Calvin cycle. Passing through a series of reactions, triose phosphate from Calvin cycle is converted to sucrose which is transported to sink cells and is eventually formed the amylose and amylopectin (starch constituents). After validating the model with data from a number of literatures, the results show that the structured model is a good representative of the studied system. The result of triose phosphate (DHAP and GAP) elevation due to lessening the aldolase activity is an illustration of the validation. Furthermore, the representative model was used to gain more understanding of starch production process such as the effect of CO$_2$ uptake on qualitative and quantitative aspects of starch biosynthesis.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.665-670
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• 2002

The expediency of replacing cost prohibitive and often inaccessible traditional protein supplements prompted the monitoring of hematological parameters was carried out in female goats at 0, 30, 60 and 90 days post feeding. Rumen environment was (3), respectively fed supplements containing either a leaf meal mixture (LMTM) of Leucaena leucocephala-Morus alba-Tectona grandis (2:1:1) or traditional protein supplements groundnut cake (GNC) or soybean meal (SBM) and wheat straw as basal diet. The periodic monitoring of hematological parameters was carried out in female goats at 0, 30, 60 and 90 days post feeding. Rumen environment was studied in bucks in a $3{\times}3$ switch over design. Rumen liquor was collected at 0, 2, 4, 6 and 8 h post feeding after 4 weeks of feeding. The goats fed on LMTM or GNC had similar dry matter intake (g/kg $W^{0.75}$), which was significantly (p<0.05) higher than SBM. Except for packed cell volume (PCV), none of the blood biochemical constituents (Hemoglobin, serum glucose, total protein, serum albumin (A) and globulin(G), A:G ratio, alkaline phosphatase, transaminases) varied significantly due to replacement of 50% dietary protein by LMTM throughout the experiment. GNC group had significantly higher level of PCV than other treatments. However, the level of serum total protein (p<0.01) tended to increase from 60th day onwards irrespective of dietary treatments. The average rumen pH was significantly higher (p<0.001) on SBM followed by LMTM and GNC, respectively. Total volatile fatty acid (TVFA) production was comparable in goats given LMTM or GNC supplements, the corresponding values were significantly different (p<0.001) when compared with SBM. The ammonical-N, total-N and TCA-precipitable-N (mg/100 ml SRL) did not differ significantly among dietary treatments. It may be concluded that supplementing wheat straw with LMTM based concentrate had no adverse effect on voluntary intake, blood biochemical profile and rumen fermentation pattern of the goats.