• Journal of Environmental Health Sciences
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• v.30 no.4
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• pp.347-351
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• 2004

A battery of sediment bioassays was performed for the sediments from the intertidal flat zone along the middle west coast of Korea to assess their potential toxicity. In the bioassays, three crustaceans, Daphnia magna, Nitocra spines, and Hyalella aztec a were exposed to $16\%$ sediments (wet weight) collected from 14 sites. Immobility($\%$) was checked as an endpoint after 24- and 48-h exposure of Daphnia magna and after 96-h exposure of Hyalella azteca and Nitocra spines. Among the three bioassays, the 48-h Daphnia bioassay showed the most distinct differential sensitivity in relation to sediment contamination, while the Nitocra and the Hyalella bioassays failed to show the differential sensitivity properly among the sites classified as polluted. Significantly different levels of immobility ($\%$) were obtained between the sites classified as chemical/nutrient polluted and the sites classified as non-polluted in the Daphnia bioassays, but not in the Nitocra bioassay and the Hyalella bioassay. Some differences of toxic response to the same sediments among bioassays were observed, suggesting that there may be a chemical specificity of response sensitivity to sediment toxicity, due to differences in bio-availability of sediment toxicants among test species.

• Environmental Analysis Health and Toxicology
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• v.14 no.4
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• pp.135-144
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• 1999

Environmental toxicities of priority water pollutants were evaluated by two selected bioassays, Lettuce seed germination/elongation test and Microtox acute toxicity test. Toxic chemicals (heavy metals, polycyclic aromatic hydrocarbons, and phenolic compounds) inhibited the germination rate and root elongation of Lettuce seed, as well as the bioluminescence of Microtox bacteria. When test biota were exposed to target chemicals, the sensitivity of Lettuce bioassay was relatively lower than that of Microtox bioassay. However, Lettuce bioassay may be a good candidate for prescreening the environmental toxicities of priority water pollutants, since the testing method with Lettuce seed was relatively easier and more economic than with Microtox bacteria. Toxicity tests were conducted to compare the validity and sensitivity of both bioassays for sediment from a small stream passed through urban area as well as leachate from a municipal solid waste landfill. From experimental results, we found that Lettuce test and Microtox test are compensated each other as a battery of bioassay for evaluating the environmental toxicities of field samples obtained from a small stream contaminated by pollutants.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.10a
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• pp.157-157
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• 2002

본 연구에서는 한강을 세 구간으로 구분하여 구간별 대표지점(의암호, 팔당호, 및 동호대교 정점)의 하상퇴적물을 grap으로 각각 채취한 후, Tandem Bioassays 을 적용하여 환경독성 수준을 평가하고 정점간 상호 비교하였다. Tandem Bioassays 이란 유해물질 오염에 의한 다양한 생태계 반응을 표현할 수 있는 최소한의 상호 보완적 환경독성 평가 체계를 의미한다. 본 연구에서 적용한 Tandem Bioassays 은 발광성미생물을 사용하는 Microtox 시험, 수서 물벼룩인 daphnia와 ceriodaphnia 시험, 그리고 개구리 배아를 이용하는 기형유발능 시험(최기형성 시험, FETAX)등으로 구성하였다.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2002.10a
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• pp.160-160
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• 2002

호소 수질에 영향을 미치는 대표적 인자로 외부 유입물질과 퇴적물을 들 수 있다. 따라서, 호소 수질 관리를 위해서는 호소로 유입되는 점ㆍ비점 오염원 관리와 함께, 퇴적층 관리도 수반되어야만 한다. 특히, 호소수가 주요 식수원으로 사용되는 경우에는 엄격한 퇴적토질 평가가 이루어져야 할 것이다. 본 연구에서는 소양호 퇴적층에 대한 환경지질학적 조사 연구의 일환으로 다양한 bioassays 기법(the battery of bioassays)을 적용하여 퇴적토의 오염상태를 평가하였다.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.8
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• pp.795-801
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• 2010

The acute toxicity of arsenic compounds was assessed and compared using following four bioassays; bioluminescence activity of the recombinant strain RB1436, germination of four different seeds, ${\alpha}$-glucosidase activity produced by Bacillus lichemiformis, acute genetic revertant mutation using mutant strain Salmonella typhimurium. Different sensitivities were observed among tested bioassays, but generally the toxicity by arsenite was greater than that of arsenate. Among tested four seeds, sensitivities of Lactucus and Raphanus were greater than others, and these two seed types were appeared as proper type for bioassay. High revertant mutation ratio (5.1) was observed with 1 mg/L arsenite, indicating high mutagenicity. The sensitivity of ${\alpha}$-glucosidase activity on arsenic compounds was much lower than other methods. The evaluation of interactive toxic effects using various bioassays may comprise a useful tool for the bioassessment of environmental pollutants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.s01
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• pp.4-15
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• 1989

The objective of this paper is to compare and summarize the procedure and effectiveness of some bioassay systems and to point out ways to obtain reliable results from each bioassay. Detailed C:escriptions were given for those widely-adapted bioassay methods, such as mungbean rooting (auxin), Avena first internode straight growth (auxin), dwarf rice growth (gibberellin), dwarf pea epicotyl elongation (gibberellin), radish cotyledon expansion test (cytokinin), and tobacco stem pith callus growth (cytokinin), and the effects of various plant growth regulators including some recently introduced growth retardants (Paclobutrazol, Uniconazol, etc.) were also summarized.

• Proceedings of the Korea Society of Environmental Biology Conference
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• 2004.05a
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• pp.1-15
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• 2004

Higher plants can be valuable genetic assay systems for monitoring environmental pollutants and evaluating their biological toxicity. Two assays are considered ideal for in situ monitoring and testing of soil, airborne and aqueous mutagenic agents; the Tradescantia stamen hair assay for somatic cell mutations and the Tradescantia micronucleus assay for chromosome aberrations. Both assays can be used for in vivo and in vitro testing of mutagens. Since higher plant systems are now recognized as excellent indicators and have unique advantages over in situ monitoring and screening, higher plant systems could be accepted by regulatory authorities as an alternative first-tier assay system for the detection of possible genetic damages resulting from the pollutants or chemicals used and produced by industrial sectors. It has been concluded that potential mutagen and carcinogen such as the heavy metals among indoor air particulates, volatile compounds in the working places, soil, and water pollutants contribute to the overall health risk. This contribution can be considerable under certain circumstances. It is therefore important to identify the level of genotoxic activity in the environment and to relate it to the biomarkers of a health risk in humans. The results from the higher plant bioassays could make a significant contribution to assessing the risks of pollutants and protecting the public firom agents that can cause mutation anuor cancer. The plant bioassays, which are relatively inexpensive and easy to handle, are recommended for the scientists who are interested in monitoring pollutants and evaluating their environmental toxicity to living organisms.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.6
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• pp.400-406
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• 2000

Since the 1950s, the numbers of species and chemicals produced have significantly increased. Despite the fact that industrial chemicals have given us numerous benefits, there is no doubt that they have damaged the environment. The chemicals being dispersed on the earth should be carefully controlled to prevent adverse effects. Bioassay is one of the methods to assess chemical safety. In bioassay systems, chemical safety is estimated by monitoring biological responses to environmental pollutants and newly synthesized chemicals. This report introduces multiple-point bioassay systems that are based on chemical sensitivities of microorganisms, responses of one kind of organism, and micro-array technology. Multiple-end-point bioassays enable the prediction of chemicals in the environment and the understanding of toxicities of newly synthesized chemicals.

• Journal of Korean Society on Water Environment
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• v.28 no.4
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• pp.551-560
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• 2012

The objectives of this study were to elucidate spatio-temporal heterogeneity of water chemistry and develop empirical models using trophic variables in Daechung Reservoir during 2005-2010 along with in situ tests of nutrient enrichment bioassays (NEB). The relations of water quality parameters in regard to precipitation showed that seasonal and interannual fluctuations of biological oxygen demand (BOD), total nitrogen (TN) and pH were minor, whereas conductivity, suspended solids (SS), and total phosphorus (TP) were largely varied in response to the magnitude of rainfall. The CHL maxima occurred immediately after the spate of TP during the high flow, indicating that phytoplankton growth was directly controlled by phosphorus. Empirical linear models of CHL-TP indicated that the variation of CHL in premonsoon was accounted 60% ($R^2$ = 0.60, p < 0.05, n = 54) by TP. In the mean time, empirical models of annual CHL-TN showed that the variation of CHL was weakly accounted ($R^2$ = 0.16, p < 0.001) by TN and more strongly ($R^2$ = 0.44, p < 0.001) by TP. Thus, the variation of CHL was more explained by the variation of TP than TN. In situ tests of Nutrient Enrichment Bioassays (NEBs) showed that the growth of CHL was greater in the P-treatments (as $PO_4-P$) than the control and N-treatment (as $NO_3-P$). Overall, our results suggest that phosphorus was aprimary limiting nutrient controlling the seasonal phytoplankton growth, based on the in situ experiments of NEBs.

• The Korean Journal of Malacology
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• v.22 no.2
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• pp.167-173
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• 2006

The embryos of marine bivalves have been commonly used in bioassays for quality assessments of marine environments. Although several standard protocols for the developmental bioassay of bivalves have been proposed, only a few trials for application of these protocols in environmental assessments or for the development of a new protocol with Korean species have been conducted. As such, there is a strong need to establish standard bioassay protocols with bivalves commonly found in Korean waters. To determine the sensitivity of Mytilus galloprovincialis to establish a standard bioassay, their fertilized eggs were exposed to six metals (Ag, Cd, Cr, Cu, Ni, and Zn). The order of biological impact was Ag > Cu > Ni > Zn > Cr > Cd and their lowest observed effective concentration were 5, 16.4, 25.4, 142, 187 and 1,500${\mu}g/l$, respectively. The proportion of normal larvae appeared to decrease linearly with the logarithm of each toxicant concentration within the tested range. The average values of median effective concentrations $(EC_{50})$ from the triplicate experiments for Ag, Cd, Cr, Cu, Ni, and Zn were 6.8, 1,797, 786, 16.6, 68.1, and 139.2${\mu}g/l$, respectively. There was a more than 100-fold difference in $EC_{50}$ values of Cu and Cd. The value of $EC_{50}$ or median lethal concentration of Cu was within the range observed for other bivalve developmental bioassays. The overall sensitivity of M. galloprovincialis in the present developmental bioassay was also similar to that of other marine organisms commonly used in aquatic bioassays (e.g. sea urchins, oysters). Hence, the bioassay using the embryo of M. galloprovincialis is considered to be a useful tool to monitor and evaluate the quality of marine aquatic environments.