• Journal of the Korea Organic Resources Recycling Association
• /
• v.21 no.4
• /
• pp.72-81
• /
• 2013

The objective of this study was to verify whether SCB(Slurry Composting & Bio-filtration) system can be applied for the treatment of anaerobic digestion(AD) wastewater and also, to identify the most effective set among three filtration compost beds tested. Results can be summarized as these; (a) When AD wastewater was sprayed on the top of beds which were mainly composed of sawdust and/or other media and, subsequently, filtrates collected and analyzed, there were large drop in the values of Electric Conductivity(EC), Total Suspended Solid(TSS), Biochemical Oxygen Demand(BOD), and Chemical Oxygen Demand(COD). In contrast, Total Nitrgen(T-N) and Total Phosphorus(T-P) were progressively elevated. We consider these changes as positive if the filtrate are to be utilized as liquid fertilizer. (b) When three sets of filtration beds (T1, T2, T3) were compared for their effectiveness, no significant difference was found among them. These indicate that expensive sawdust can be replaced in part with cheaper media such as woodchip, rice husks, or others. (c) At early stage of operation (within 20 days), BOD in filtrates were maintained at high level probably due to the lack of microbial activity. During the same stage, T-N, T-P was at low level but, were elevated to higher levels thereafter. These data, when combined, indicate that the filtration system needs at least a couple of weeks for the optimized microbial functioning. (d) The temperatures of the experimental beds were progressively dropped as the experiment continued through the fall season, although filtration effectiveness was not noticeably influenced.

• Korean Journal of Soil Science and Fertilizer
• /
• v.3 no.1
• /
• pp.55-59
• /
• 1970

In an effort to determine the bio-synthesis in the soybean as investigate to the variance of each substance: Glutamic acid, Aspartic acid and its amides during the growth of younger soybean plants. 1. The variance-curve of Gultamic acid and Aspartic acid as the acidic amino acids in the cotyledons was appeared the peak the first half period at Glutamic acid and the latter half at Aspartic acid in the growth of soybeans, and was received the symmetrical impression centering around the stage of adult leaf-development. But, in the embryonic organ, it appears the peak at both part, in the developmental stage of adult leaf and also appears near phenomena of increase and decrease in the variation-curve of metabolites. 2. It's amides-Gultamine and Asparagine-appears the peak at the developmental stage of adult leaf in the both cotyledons and embryonic organ, and rapid increase in the cotyledons were very impressed compare with the decrease at fallen stage of cotyledons in the embryonic organs. 3. In the relation of variance at Glutamic acid and Aspartic acid, both substance were discovered the fact of translocation from cotyledon to embryonic organ, and Glutamic acid could supposed that bear the charges of outrider substance in other amino acid as the Glutamic acid-self and major basic function for receiving the ammonia as the nitrogen contain constituent of plant. In the case of Glutamine, formation-mechanism of ammonia which develops due to its hydrolysis in the latter period of soybean growth, suggested that was forfeit its function till instance of fallen cotyledons. 4. In the relation the Aspartie acid and Asparagine, Aspartic acid which begins to decrease from seed-state was supposed that bear sufficiently the charge of outrider substance in the formation of Asparagine other than translocated to embryonic organ from cotyledon. And, formation-theory of Aspartic acid which suppose as formational substance from Kreb's cycle were recognized from latter period of soybean growth, and then, rapid accumulation of Asparagine's amounts were supposed that adapt to two theory: Theory which consider to transformation as Asparagine state for pressing to less than noxious weight the concentration of ammonia developing from the cells, and was formate and accumulate as ammonia or carbohydrates containing excess in the cotyledons.

• Korean Journal of Soil Science and Fertilizer
• /
• v.3 no.1
• /
• pp.49-54
• /
• 1970

In an effort of determine the metabolism and bio-synthesis of nitrogen, was studied at variance of souble nitrogens, free amino acids and total alcohol soluble amino acids during the growth of younger soybean plants, and saybean divides into cotyledon and embryonic organ (shoot and root system) in this experiment. 1. In the soluble nitrogen of soybean, ratio of increase and decrease in the amino acids of them was displayed the near phenomena both cotyledon and embryonic organ of soybean. But, in the 17th days after seed germination, that is the developmeatal stage of adult leaf of soybeans, was appear the maximum value. It has been suggested that the stage of first half period of growth as boundary the stage of adult leaf development which indicated clear morphologically, at the younger soybean plants, is the step that nitrogen assimilation. 2. It was investigated the amino acids of seventeen kinds in the seed state, but at the third days after seed germination, was investigated the amino acids of nineteen kinds. Ultimately, it appears the translocation from cotyledon to embryonic organ in the distribution of amino acid, the nineth day which differentiation begining day of embryonic organ, then after, it happen the variation of number of inspected amino acid in the cotyledon and embryonic organ but only the variance changes in the distribution and quantitative aspects. Especially, the most conspicuous fact was indicated the accumulation of Asparagine, that is the phenomena of Asparagine-accumulation was constitute, not with standing no fertilization from outside. It may be concluded from the results of this investigation that the difference of special phenomena of soybean from the embryo of other plans. 3. In the initial stage of differentiation at embryonic organ number of inspected amino acid was very few, and then, it was slightly appeared the increase-phenomena in the number of them. It was that the amino acid inspecting the initial stage was translocated from the cotyledon. It is suggested that the intermediate-metabolism of amino acid was constituted on the basis of above the result. 4. The phenomena of increase and decrease of total alcohol soluble amino acid were essentially identical to the water soluble amino acid of soybean, but it was appeared the severe difference of amounts in both of them.

• Microbiology and Biotechnology Letters
• /
• v.35 no.1
• /
• pp.1-10
• /
• 2007

The 44 endophytic bacterial strains were isolated from surface-sterilized root of rice cultivated in seven different locations of Chungcheong province, Korea. Each isolate was introduced into rice seedlings grown gnotobiotically by inoculating scissor-cut first true leaf with cell suspensions, and the colonization capacity of each isolate in root tissue was analyzed at 7 days after inoculation. Sixteen out of 44 isolates were re-isolated from root successfully with the frequency of $10^{3-5}$ CFU/g tissue. Interestingly, seven out of 16 isolates were identified as Burkholderia species. The identity between inoculated strains and re-isolates was confirmed by genomic finger-printing and 16S rDNA sequence analysis. By a confocal laser scanning microscopic observation it was revealed that KJ001 strain, one of the sixteen isolates tagged with gfp colonized in root tissue especially around xylem. Six out of seven Burkholderia strains obtained in this study showed antagonizing activities against seven different fungal pathogens, contain nifH gene, and five of them enhanced growth of cucumber over 30%. The isolates showed no hypersensitive response on tobacco leaves and no pathogenecity in rice. From these results it was found that the endophytic Burkholderia strains will be useful in agriculture to develop a biocontrol agent or a bio-fertilizer.

• Horticultural Science & Technology
• /
• v.35 no.3
• /
• pp.289-299
• /
• 2017

The objective of this study was to determine the effect of replacing perlite (PL) with pellets processed with poultry feather fiber as an inert material to prepare growing medium. The growth and flowering of Salvia splendens 'Vista Red' grown in individual growing medium $Biosangto^{(R)}$, peat moss (PM), PL, and two pellets (P45-1 and P45-2) were evaluated. Peat moss was mixed with PL, P45-1, or P45-2 at various ratios (1:0 to 1:3 or 3:1 by volume) to investigate the feasibility of replacing PL with pellets. Nutrient composition of the growing medium and leaf tissues was analyzed. The number of florets, inflorescence length, plant height, and fresh weight of plants grown in media containing P45-1 or P45-2 were reduced compared to those grown in individual growing medium PM or PL. As the mixing ratio of P45-1 or P45-2 to PM was higher, the growth of salvia, such as inflorescence length, plant height, number of leaves, and fresh weight was inhibited. Our results indicate that mixing three parts PM with one part of P45-1 (PM/P45-1/3:1) or P45-2 (PM/P45-2/3:1) accelerated flowering and increased the number of florets and leaves compared to other mixing ratios of PM and pellets media. The concentrations of phosphorus (P), calcium (Ca), boron (B), iron (Fe), and copper (Cu) in individual growing medium PL, P45-1, and P45-2 were significantly lower than those in PM. The concentration of N was the highest in leaves of plants grown in P45-1 or P45-2 amended media, and the concentrations of P, Ca, and zinc (Zn) in leaves were lower in individual growing medium P45-1 or P45-2 than in PM and PL. The pH of PM/P45-1/3:1 or PM/P45-2/3:1 media was maintained at optimal level (5.8-5.9) and the concentrations of macro- and micro-elements in the media and leaves were considered to be optimal levels. Therefore, mixing three parts PM with one part P45-1(PM:P45-1/3:1) or P45-2 (PM:P45-2/3:1) is recommended for improved growth and flowering in salvia. This suggests that P45-1 or P45-2 can replace PL as an inert material to prepare growing medium.

• Journal of Life Science
• /
• v.24 no.12
• /
• pp.1308-1315
• /
• 2014

Biological control using the plant growth-promoting Rhizobacterium (PGPR) has received significant attention in recent years. PGPR has been linked with promoting growth in economically important crops, such as potatoes, tomatoes, and rice. Bacillus mojavensis KJS-3 (Moja-3), isolated from food waste, possesses antifungal properties against Aspergillus terreus, A. fumagatus, A. flavus, and Fusarium redolense, and it may have potential in the development of products for industrial applications. The main purpose of this study was to determine the effects of spraying the PGPR Bacillus mojavensis KJS-3 on the growth of altari radish (leaf number, leaf length, leaf weight, root length, and rhizome length, adjacent portion diameter, and weight) and lettuce (leaf number, length, width, and weight). Three different concentrations of the foliar spray treatment of B. mojavensis KJS-3 were applied to the altari radish and lettuce: the recommended standard concentration of $1{\times}10^9cfu/g$, half the standard concentration of $0.5{\times}10^9cfu/g$, and double the standard concentration of $2{\times}10^9cfu/g$). The B. mojavensis strain foliar spray treatment increased the growth of the leaves and roots of the altari radish and increased the growth of the lettuce leaves. For both plants, the recommended concentration of B. mojavensis KJS-3 produced better growth than half the standard concentration, and the growth was similar with the double dose. This study demonstrates positive effects of Moja-3, suggesting it may be a potential new bio-fertilizer for improving the growth of altari radish and lettuce.

• Journal of Animal Environmental Science
• /
• v.1 no.1
• /
• pp.55-64
• /
• 1995

A wastewater purifying system using phototrophic bacterium, Rhodopseudomonas capsulata, is currently in operation in several countries, One of them, is a continuously aerated bioreactor(CABR) system, which treats concentrated swin wasterwater using small amounts of phototrophic bacteria as additive bacterial seeding. Using this plant, total biochemical oxygen demand was decreased to 13%, and most of volatile fatty acids were removed. About 40% of the wastewater(Influx) was evaporated during aerobic digestion for 24h, and 60% of that erupted in a decodorized foam(Efflux). The efflux had enough nutrients, N, P and K kor growing plant, as well as organic matters. When the efflux was applied to Italian ryegrass with high dose, fresh shoot and root weights were significantly greater, and $NO_3-N$ contents of the dried shoot were lower than those of control plant (CDU). These results indicate that the CABR plant is useful for reduction and deodorization of swine wastewater and the efflux from CABR can be used for crop production as an organic fertilizer.

• Korean Journal of Environmental Agriculture
• /
• v.39 no.1
• /
• pp.58-64
• /
• 2020

BACKGROUND: Most of the researches on the dye removal using ozonation have been focused on the removal efficiency. However, the research on their removal characteristics and mechanism according to the reaction time has been still insufficient. METHODS AND RESULTS: In this study, the effects of initial pH and dye concentration with reaction time on the degradation characteristics of methyl orange (MO) and methylene blue (MB) by ozonation were evaluated. The degradation efficiency of MB by ozonation increased with increasing pH. On the other hand, the degradation efficiency of MO by ozonation did not show a significant difference with varing pH. The both MO and MB by ozonation were decomposed within 30 min irrespective of the dye concentration, but the decomposition rates of dyes were faster at lower initial dye concentration. The decomposition efficiency of total organic carbon (TOC) in each dye solution by ozonation was low, which was found to be effective for partial decomposition such as decolorization rather than complete degradation of the dye. CONCLUSION: Overall, ozonation was an effective method for removing nondegradable dyes. However, it is necessary to study the optimization of dye degradation under various environmental conditions for ozonation.

• Korean journal of applied entomology
• /
• v.44 no.4 s.141
• /
• pp.307-315
• /
• 2005

In this study we tried to develop a biological method for evaluating environment using the potter wasps as an indicator. Wasp species in family Eumenidae, were collected from 13 locations including agricultural area using nest trap. The wasps collected were classified and selected candidate species as an environmental evaluation indicator. Seven species were collected and the Anterhynchium flavomarginatum was the most dominant species. The number of the nest of potter wasps was 12.8/m both in the non-fertilizer and the non-pesticide areas and 7.2/m in the general agricultural areas. The number of nests was 13.4/m in the location where the Degree of Green Naturality (DGN) was high (4.28) and it was 1.2/m where the DGN was low (1.00), suggesting that the index of both richness and diversity tend to increase in locations with more nests. Based on these results, A. flavomarginatum, Orancistrocerus drewseni (Saussure), Isodontia nigellus and Chalicodoma sculpturalis were selected as indicator species for the evaluation of environment including agricultural ecosystem. And a standard for grading an environment (I to IV) was made based on the occurrence, the total number of nesting and the species diversity of potter wasps.

• Korean journal of applied entomology
• /
• v.46 no.3
• /
• pp.335-341
• /
• 2007

The main habitats of diving beetles in agricultural ecosystems were identified as ponds, irrigation channels, and reservoirs, where the water system is maintained throughout the year. Four species, Cybister japonicus, Cybister brevis, Hyphydrus japonicus and Noterus angustulus, were selected as biological indicators which can be used to evaluate the healthiness of the agricultural ecosystem. The species number of 4 indicator species, the species number of diving beetle species, and the diversity index were used as factors for environmental evaluation. The evaluation was classified into grades $I{\sim}IV$. The non-fertilizer and non-pesticide agricultural practicing area and the sustainable agricultural practicing area were evaluated as grades $I{\sim}II$, and the general agricultural area to be relatively fine with a grade II. However, the analysis indicated the agricultural areas near a residential area and an industrial complex to be poor with a grade of IV, suggesting that immediate improvement in the agricultural environment is needed.