• Title/Summary/Keyword: bio-KI

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Implementation of Impedance Method to Estimate Blood Flow Variation with Cuff Pressure Change (커프 압력 조절에 따른 혈류량 변화 평가를 위한 임피던스법의 구현)

  • Jeong, Do-Un;Bae, Jin-Woo;Shon, Jung-Man;Yae, Su-Yung;Choi, Byeong-Cheol;Nam, Ki-Gon;Kim, Cheol-Han;Jeon, Gye-Rok
    • Journal of Sensor Science and Technology
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    • v.13 no.6
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    • pp.462-472
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    • 2004
  • In this study, we measured the blood flow on arm by non-invasive method and implemented a system to measure variation of the blood flow by estimating bio-electrical impedance and arterial pressure according to cuff pressure. The implemented system measured impedance variation according to pressure variation applied by artificial cuff pressure on the measuring position. The system consisted of pressure measuring part and impedance measuring part using 4-electrode method. Pressure measuring part was composed of semiconductor pressure sensor and electronic circuit for signal processing of sensor output signal. In addition, impedance measuring part was composed of constant current source circuit and lock-in amplifier for detecting impedance signal. We conducted experiments of impedance measuring part using standard resistance for performance evaluation of the implemented system. In addition we experimented to estimate variation of the blood flow by measuring impedances of the experimental group. We estimated ratio of the blood flow resistance using mean arterial pressure and variation of the blood flow. As a result the ratio of the blood flow resistance and variation of blood flow were in an inverse relationship with each other and the correlation coefficient was -0.96776.

Facile Synthesis of Dendritic-Linear-Dendritic Materials by Click Chemistry

  • Lee, Jae-Wook;Han, Seung-Choul;Kim, Byoung-Ki;Lee, Un-Yup;Sung, Sae-Reum;Kang, Hwa-Shin;Kim, Ji-Hyeon;Jin, Sung-Ho
    • Macromolecular Research
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    • v.17 no.7
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    • pp.499-505
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    • 2009
  • General, fast, and efficient stitching methods are presented for the synthesis of Fr$\acute{e}$chet-type dendrimers with linear units at a core, as a preliminary investigation for the synthesis of dendritic-linear-dendritic materials. The synthetic strategy involved an inexpensive, 1,3-dipolar, cycloaddition reaction between an alkyne and an azide in the presence of the Cu(I) species, which is known as the best example of click chemistry. The linear core building blocks, 1,7-octadiyne and 1,6-diazidohexane, were chosen to serve as the alkyne and azide functionalities for dendrimer growth via click reactions with the azide and alkyne-dendrons, respectively. These two building blocks were employed together with the azide- and alkyne-functionalized Fr$\acute{e}$chet-type dendrons in a convergent strategy to synthesize two kinds of Fr$\acute{e}$chet-type dendrimers with different linear core units. This comparative efficiency of the click methodology supports the fast and efficient synthesis of dendritic-linear-dendritic materials with the tailor made core unit.

PREVALENCE OF BLACK-PIGMENTED BACTERIA IN INFECTED ROOT CANALS IN KOREA (감염 근관의 흑색세균의 동정)

  • Chung, Ki-Soo;Lim, Sung-Sam;Bae, Kwang-Shik
    • Restorative Dentistry and Endodontics
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    • v.24 no.3
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    • pp.447-452
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    • 1999
  • The role of bacteria in root canals and periapical infections is well known and established. In these bacteria, black-pigmented bacteria(BPH) play important role in endodontic infection. BPB are Gram negative anaerobic rods which are closely related 50 clinical symptoms such as pain, percussion, tenderness, foul odor, etc. In America and Europe, many studies on BPB have been done and are continued. But, relatively few studies have been done in Korea, especially its prevalence in Korean population is not yet studied. The purpose of this study is to establish prevalence of BPB in infected root canals and periapical abscesses in Korean people. Microbial samples were collected from the root canals of 34 intact tooth with periapical rarefactions of endodontic origin and 3 periapical abscesses. All samples were incubated in an anaerobic chamber(Coy, Model No. 77. Ann Arbor, Michigan, USA.). Identification of In microorganism was based on its growth in the anaerobic chamber, colonial pigmentation, colonial morphology, Gram stain, and Rapid ID32A(BioMericux SA/69280 Marcy-l'Etoile/France) results. In addition, the polyme ase chain reaction using specific primers for 16S rRNA genes were used differentiate Prevotella nigrescens for Prevotella intermedia. The results were as follows : 1. In this study, thirteen (35%) of thirty seven samples were positive for the growth of BPB. In thirteen samples, sixteen strains of BPR were found. 2. The most frequently identified BPB in root canals and abscesses in Korean were P. nigrescens 5/37(14%) and P. intermedia 5/37(14%). Porphyromonas gingivalis 3/37(8%), Porphyromonas endodontalis 2/37(5%) and Prevotella loecheii 1/37(3%) were also found. 3. In this study, no significant differences were found between the prevalence of BPB in Korean and that of American and European.

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Comparison of optical reflectance spectrum at blade and vein parts of cabbage and kale leaves

  • Ngo, Viet-Duc;Ryu, Dong-Ki;Chung, Sun-Ok;Park, Sang-Un;Kim, Sun-Ju;Park, Jong-Tae
    • Korean Journal of Agricultural Science
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    • v.40 no.2
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    • pp.163-167
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    • 2013
  • Objective of the study was to compare reflectance spectrum in the blade and the vein parts of cabbage and kale leaves. A total 6 cabbage and kale leaves were taken from a plant factory in Chungnam National University, Korea. Spectra data were collected with a UV/VIS/NIR spectrometer (model: USB2000, Ocean Optics, FL, USA) in the wavelength region of 190 - 1130 nm. Median filter smoothing method was selected to preprocess the obtained spectra data. We computed reflectance difference by subtraction of averaged spectrum from individual spectrum. To estimate correlation at different parts of cabbage and kale leaves, cross - correlation method was used. Differences between cabbage and kale leaves are clearly manifested in the green, red and near - infrared ranges. The percent reflectance of cabbage leaves in the NIR wavelength band was higher than that of kale leaves. Reflectance in the blade part was higher than in the vein part by 18%. Reflectance difference in the different parts of cabbage and kale leaves were clear in all of the wavelength bands. Standard deviation of reflectance difference in the vein part was greater for kale, while the value in the blade part was greater for cabbage leaves. Standard deviation of cross - correlation increased from 0.092 in the first sensor (UV/VIS) and 0.007 in the second sensor (NIR) to 0.099 and 0.015, respectively.

Morphological and Phylogenetic Characteristics of Nematophagous Fungi (식물기생성 선충 포식곰팡이의 형태 및 계통분류학적 특성)

  • Kang, Doo-Sun;Jeon, Han-Ki;Son, Hee-Seong;Whang, Kyung-Sook;Cho, Cheon-Whi
    • Applied Biological Chemistry
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    • v.50 no.2
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    • pp.101-106
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    • 2007
  • Twenty-two strains of nematophagous fungi were isolated from 100 soil samples. Nematophagous fungi were classified into three categories; 3-dimensional adhesive nets (A group), 2-dimensional adhesive nets (B group) and constricting ring (C group). Nine strains were selected and identified on the basis of morphological characteristics (hypha, conidiophore, form and size of conidia, number of conidia, node of conidophore, number and location of septa, size and color of chlamydospore) and ITS (internal transcribed spacer) region of rDNA sequences. As the results, the isolated were identified as belonging to the species of Monacrosporium thaumasium (Kan-2, Kan-4, Kan-11), Arthrobotrys oligospora (Kan-9, Kan-13, Kan-20, Kan-21), A. musiformis (Kan-12), and A. dactyloides (Kan-22).

The Anti-Adipogenic Activity of a New Cultivar, Pleurotus eryngii var. ferulae 'Beesan No. 2', through Down-Regulation of PPAR γ and C/EBP α in 3T3-L1 Cells

  • Kang, Min-Jae;Kim, Keun Ki;Son, Byoung Yil;Nam, Soo-Wan;Shin, Pyung-Gyun;Kim, Gun-Do
    • Journal of Microbiology and Biotechnology
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    • v.26 no.11
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    • pp.1836-1844
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    • 2016
  • Adipogenesis is one of the cellular processes and a highly controlled program. Nowadays, inhibition of adipogenesis has received attention as an effective way to regulate obesity. In the current study, we investigated the inhibition effect of a chloroform extract of Pleurotus eryngii var. ferulae 'Beesan No. 2' (CEBT) on adipogenesis in 3T3-L1 murine preadipocytes. Pleurotus eryngii var. ferulae is one of many varieties of King oyster mushroom and has been reported to have various biological activities, including antitumor and anti-inflammation effects. Biological activities of 'Beesan No. 2', a new cultivar of Pleurotus eryngii var. ferulae, have not yet been reported. In this study, we found that CEBT suppressed adipogenesis in 3T3-L1 cells through inhibition of key adipogenic transcription factors, such as peroxisome proliferatoractivated receptor ${\gamma}$ and CCAAT/enhancer binding protein ${\alpha}$. Additionally, CEBT reduced the expression of the IRS/PI3K/Akt signaling pathway and its downstream factors, including mammalian target of rapamycin and p70S6 kinase, which stimulate adipogenesis. Furthermore, ${\beta}-catenin$, a suppressor of adipogenesis, was increased in CEBT-treated cells. These results indicate that Pleurotus eryngii var. ferulae 'Beesan No. 2' effectively inhibited adipogenesis, so this mushroom has potential as an anti-obesity food and drug.

Efficacy of Recombinant Baculovirus Vector Reconstructed in pcDNA3.1 Vector (pcDNA3.1 벡터에서 재구성된 재조합 Baculovirus 벡터의 효능)

  • Sa, Young-Hee;Choi, Chang-Shik;Lee, Ki Hwan;Hong, Seong-Karp
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.10a
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    • pp.444-447
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    • 2018
  • Baculovirus expression systems have many known advantages including fast and cost-effective methods to generate large amounts of recombinant proteins in comparison to bacterial expression systems, particularly those requiring complex post-translational modifications. Especially, recombinant baculoviruses can transfer their vectors and express their recombinant proteins in a wide range of mammalian cell types. In this study, baculoviral vectors which were reconstructed from pcDNA3.1 vector, were recombined with cytomegalovirus (CMV) promoter,uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD). These recombinant vectors were infected with various cells and cell lines. The baculovirus vector thus developed was analyzed by comparing the metastasis and expression of the recombinant genes with conventional vectors. These results suggest that the baculovirus vector has higher efficiency in metastasis and expression than the control vector.

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Technical Characteristics and Trends of Capsule Endoscope (캡슐 내시경의 기술적 특징과 동향)

  • Kim, Ki-Yun;Won, Kyung-Hoon;Choi, Hyung-Jin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37 no.4C
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    • pp.329-337
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    • 2012
  • Capsule Endoscope(CE) is a capsule-shaped electronic device which can examine the lesions in digestive tract of human body. Recently the medical procedure using capsule endoscope is receiving great attention to both doctors and patients, since the conventional push-typed endoscope using cables brings great pain and fear to the patients. The technique was firstly available in 2000 and is based on a convergence techniques among BT(Bio Technology), IT(Information Technology), and NT(Nano Technology). The device consists of an optical parts including LEDs(Light Emitting Diodes), an image sensor, a communication module and a power module. Capsule endoscope is the embodiment of the state-of-the art technology and requires key technologies in the various engineering fields. Therefore, in this paper, we introduce the composition of the capsule endoscope system, and compare the communication method between RF(Radio Frequency) communication and HBC(Human Body Communication), which are typically used for data transmission in the capsule endoscope. Furthermore, we analyze the specification of commercialized capsule endoscopes and present the future developments and technical challenges.

Evaluation of DES key search stability using Parallel Computing (병렬 컴퓨팅을 이용한 DES 키 탐색 안정성 분석)

  • Yoon, JunWeon;Choi, JangWon;Park, ChanYeol;Kong, Ki-Sik
    • Journal of Digital Contents Society
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    • v.14 no.1
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    • pp.65-72
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    • 2013
  • Current and future parallel computing model has been suggested for running and solving large-scale application problems such as climate, bio, cryptology, and astronomy, etc. Parallel computing is a form of computation in which many calculations are carried out simultaneously. And we are able to shorten the execution time of the program, as well as can extend the scale of the problem that can be solved. In this paper, we perform the actual cryptographic algorithms through parallel processing and evaluate its efficiency. Length of the key, which is stable criterion of cryptographic algorithm, judged according to the amount of complete enumeration computation. So we present a detailed procedure of DES key search cryptographic algorithms for executing of enumeration computation in parallel processing environment. And then, we did the simulation through applying to clustering system. As a result, we can measure the safety and solidity of cryptographic algorithm.

Building of an Vessel Operator Support System Based on ENC (ENC기반 선박운항자 지원시스템의 구축)

  • Hong Tae-ho;Seo Ki-Yeol;Park Gyei-Kark
    • Proceedings of KOSOMES biannual meeting
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    • 2005.05a
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    • pp.135-140
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    • 2005
  • Currently, ECDIS and GPS plotters are much used as equipment providing operators with route information, but they do not have any function of automatic route creation and route explanation, so available for only experienced operators. Especially, the present situation is tint no study is made of the automatic route creation and route explanation using ENC for ECDIS, substitution system of paper chart. ENC is the electronic navigation chart that is produced using S-52, S-57 standard format required by IHQ. In this paper, an Vessel Operator Support System(VOSS) is proposed to generate an optimal route where ENC and GPS data is fusioned induding the wind direction and speed of an anemometer and tide data. The proposed system was testified by a simulation, and its effectiveness was verified.

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