• 한국초지조사료학회지
• /
• 제24권4호
• /
• pp.341-346
• /
• 2004

버즈풋 트레포일(Lotus corniculatus L.) 및 이탈리안 라이그라스(Lolium multiflotrum L.) 의 치사온도를 결정하기 위하여, 각각 "Au Dewey"와 국내 육성품종인 "화산 101호" 품종을시험재료조 하여 종자를 Petri dish에서 발아시켜 작은 화분에 10 개체씩 이식, 생장실에서 4주간 재배하였다. 45, 50 및$ 55^{\circ}C$에서 처리한 경우에는 60분간 처리했을 때에도 거의 식물체손상이 없었다. $60^{\circ}C/60$분 처리에서는 잎이 약간 시든 듯한 현상이 있었으며, $65^{\circ}C/60$분 처리에서도 식물체의 심한 손상은 나타나지 않았다. $70^{\circ}C/60$분 처리에서 BFT의 일부에서 잎이 조금 마른 현상이 나타났고, IRG는 줄기 이외의 잎 부분이 거의 마른 상태가 되었지만 치사 온도에는 도달하지 않았다.$ 80^{\circ}C$에서 처리한 BFT는 처리 후 1일 이내에 60분 처리에서 잎이 심하게 시든 현상이 나타났다. 처리 후 6일에는 60분 처리에서 모두 죽었으며, 55분 이하의 처리에서는 새로운 shoot가 재생됨을 확인하였다. $80^{\circ}C$에서 처리한 IRC는 처리 후 1일 이내에 20분 이상 처리에서 거의 죽어가는 현상이 나타났다. 처리 후 7일에는 20분 이상의 처리에서 모두 죽었으며, 15분 이하의 처리에서는 심한 영향을 받지 않았으며 새로운 shoot가 재생됨을 확인하였다. 결론적으로 버즈풋 트레포일의 치사온도는 $80^{\circ}C/60$분, 이탈리안 라이그라스의 치사온도는 $80^{\circ}C/20$분으로 결정되었다. 다른 연구에서 형질전환 버즈풋 트레포일 및 이탈리안 라이그라스를 만들 경우, 형질전환체의 고온 내성을 간단하게 검정하는 방법으로 이상의 치사온도 결과를 이용할 수 있을 것으로 사료된다.

• Journal of the Korean Data and Information Science Society
• /
• 제22권6호
• /
• pp.1145-1152
• /
• 2011

지금까지 한우의 유전능력 개량은 수소 위주로 이루어져 왔으나, 최근 암 수소 동시개량을 위하여 많은 연구가 진행 중에 있다. 본 연구에서는 암소의 유전능력 개량을 위하여 생체 초음파 단층 촬영기법을 이용하여, 주요 경제형질에 대한 유전적 효과를 측정하였다. 한우 암소 1,648두의 생체 초음파 단층촬영 결과를 이용하여 측정시 암소의 사육월령, 측정지역, 신체충실지수의 효과를 일반 선형모형을 이용하여 조사하였다. 한우 암소의 초음파 측정시 사육월령별 초음파 성적은 50~60개월령의 등심단면적, 등지방두께, 근내지방도 성적에서 유의적으로 높게 나타나 이 시기가 암소의 발육이 가장 높은 시기로 판단된다 (P<0.05). 그리고 지역별 암소의 초음파 성적은 C 지역이 신체충실지수, 등심단면적, 등지방두께가 높게 나타났으며, J지역은 근내지방도가 가장 낮게 나타났다 (P<0.05). 신체충실지수가 높을수록 등심단면적, 등지방두께, 근내지방도 성적이 높게 나타났다. 제한최대우도법으로 유전모수를 추정한 결과 유전력은 등심단면적이 0.136, 등지방두께가 0.351, 근내지방도가 0.236으로 나타났다. 이러한 초음파를 이용한 유전모수 추정결과는 수소 거세우의 도체성적을 이용한 유전모수 추정결과에 비하여 낮게 나타났지만, 도축을 하지 않은 암소의 유전능력 개량을 위해서는 반드시 필요한 기초자료라 판단된다.

• 한국환경농학회지
• /
• 제42권4호
• /
• pp.408-417
• /
• 2023

This study aimed to investigate the effects of an aquaponics system utilizing catfish recirculating water on the growth and water quality in lettuce and chicory cultivation. After 60 d of catfish cultivation in the BFT-AP, the survival rate was 96.0% and average weight gain was 178.5 g. The WGR grew by 241.4% and the SGR was 3.0%. A comparison between lettuce and chicory cultivation using BFT-AP and hydroponics revealed significant increases in shoot height, leaf count per week, and biomass of the cultivated lettuce and chicory. The SPAD values also increased significantly by 15.4-43.3%. Water quality analysis of the BFT-AP systems and crop cultivation beds showed significant differences, except for K and Na. The average NO₃^- concentration in the sediment pond was significantly higher at 45.3±1.0. Comparison of water temperature, pH, EC, DO, and ORP in the BFT-AP system and crop cultivation beds revealed similar monthly water temperatures from July to September. The pH in the catfish culture pond was 0.3-1.2 lower than that in the crop cultivation beds, and the EC was approximately 0.01- 0.03 dS/m lower in the catfish culture pond than the crop cultivation beds. The monthly average ORP in the catfish culture bed steadily increased to 479.9 mV in October. Bacillus velezensis (AFB2-2) and Chlorella fusca (CF) were applied to the biological activation bed; the population density of CF showed a continuous increase, whereas that of AFB2-2 decreased after 15 d and gradually increased thereafter. Thus, the application of the recirculating aquaponics cultivation technology with catfish (BFT) enhances catfish cultivation and promotes the growth of lettuce and chicory. Therefore, it is considered suitable for the environment-friendly cultivation of lettuce and chicory.

• Journal of Animal Science and Technology
• /
• 제47권2호
• /
• pp.261-270
• /
• 2005

Total number of 237 Hanwoos were purchased from the nationwide fanns based on the different market weights and sex. After slaughtering, they were evaluated to estimate the productivity of carcass components by the sex and market weight, and to predict the beef carcass yield grade equation. The main results in this study were summarized as follows; With respect to carcass characteristics, the yield percentages were different(P < 0.01) among sex groups, but were not different among market weight groups. Sex or market weights showed significant effects on the carcass yield grade(YG) factors(BFT, RA, carcass weight(CW). The YG equation from this study could be expressed as a multiple regression y = 64.74 - 0.0198 ${\times}$ CW(kg) - 5.226 $\time$ BFT(cm)+0.1339 $\time$ RA($cm^2$ $R^2$ = 0.52 Among the YG factors, the BFT was the highest contributor to the YG equation. Compared the previous equation reported in 1992 with the equation from this study, the bias values between predicted value and the actual cutability were 9.17 and 2.39, respectively. In conclusion, the equation estimated from this study was more accurate in predicting the cutability than that from the previous equation reported in 1992.

• Journal of Animal Science and Technology
• /
• 제58권4호
• /
• pp.15.1-15.6
• /
• 2016

Background: Our previous study had identified the SNP (g.81966377T > C) and indel (g.81966364D > I) located in the promoter of APM1 to have a significant effect on marbling in Hanwoo. APM1 encodes an adipocytokine called adiponectin, which plays a significant role in lipogenesis. The aim of this study was to verify and validate the effect of the SNP and indel on marbling and other carcass traits in a large, representative, countrywide population of Hanwoo cattle. The carcass traits measured were marbling (MAR), backfat thickness (BFT), loin eye area (LEA), and carcass weight (CAW). Results: Primers were designed to amplify 346 bp of the genomic segment that contained the targeted SNP (g.81966377) and the indel (g.81966364). After data curation, the genotypes of 8,378 individuals identified using direct sequencing analysis estimated frequencies for C (0.686) and T (0.314) respectively showing genotype frequencies for CC (0.470), CT (0.430) and TT (0.098). The genotypes were significantly associated with MAR, BFT and LEA. The indel had significant effect on marbling (P < .0001) with strong additive genetic effects. The allele frequencies was estimated at (DEL, 0.864) and insertion (INS, 0.136) presenting genotypes of D/D (75.63 %), D/I (21.44 %), and I/I (2.92 %). Significant departure from Hardy-Weinberg equilibrium was not detected for both the SNP and the indel. Conclusion: The SNP genotypes showed significant association with MAR, BFT and LEA with strong additive genetic effects, while the indel was significantly associated with MAR. The results confirmed that the variants can be used as a genetic marker for improving marbling in Hanwoo.

• Journal of Animal Science and Technology
• /
• 제49권2호
• /
• pp.177-182
• /
• 2007

본 연구는 한우에서 이유시 체중과 도체형질들 간의 상관관계를 구명하고자 실시하였다. 조사된 도체 형질은 배최장근 단면적, 등지방두께, 21단계로 구분하여 평가한 근내지방도(근내지방도 I)와 7단계로 구분하여 평가한 근내지방도(근내지방도 II), 그리고 7단계로 구분하여 평가한 육색 등이었다. 유전모수의 추정은 DFREML 방법을 적용하여 실시하였는데 이유시 체중에 대한 통계모형은 동기우 그룹효과(년도－계절－성) 외에 이유시 송아지 일령 및 어미소 일령의 1차식 효과와 2차식 효과를 고정효과로 포함하였고 개체효과를 임의효과로 포함하였다. 도체형질에 대한 통계 모형은 동기우 그룹효과(년도－계절－성) 외에 도축시 일령의 일차식 효과를 고정효과에 포함하였고 개체효과를 임의효과로 포함하였다. 조사된 형질별 유전력 추정치는 이유시 체중이 0.25, 배최장근 단면적이 0.20, 등지방두께가 0.20, 근내지방도Ⅰ이 0.32, 근내지방도 Ⅱ가 0.32 그리고 육색이 0.22였다. 이유시 체중과 배최장근 단면적, 등지방두께, 근내지방도 I, 근내지방도 II 및 육색 간의 유전(표현형) 상관계수는 각각 0.75(0.16), 0.18(0.05), －0.41(－0.09), －0.40(0.11) 및 －0.07(0.05)였다. 본 연구 결과는 이유시 체중이 무거운 방향으로 단형질 선발을 진행할 경우 등심단면적이 넓어지고, 등지방두께가 두꺼워지며 근내지방도가 감소하는 도체를 생산하는 후손집단이 형성될 가능성이 있음을 시사하고 있다.

• 한국축산식품학회지
• /
• 제31권2호
• /
• pp.280-289
• /
• 2011

본 연구는 2009년도에 전국에서 출하된 한우 암소 및 거세우 69,159두를 대상으로 등지방두께가 도체등급요인 및 경락가격에 미치는 영향을 구명하였다. 평균 등지방두께는 거세우(12.57 mm)가 암소(11.93 mm) 보다 높았으며, 암소의 77.52%가 등지방두께 4-15 mm 구간에, 거세우의 85.91 %가 등지방두께 4-18 mm 구간에 속하였다. 암소 및 거세우의 도체중(평균, 315.5 kg과 413.2 kg)과 배최장근면적(평균, 76.73 $cm^2$와 86.23 $cm^2$)은 등지방두께가 높아짐에 따라 증가 하였으며(p<0.05), 육량등급은 C등급으로 낮아졌다(p<0.05). 평균 근내지방도는 거세우(5.29)가 암소(3.83)보다 우수하였으며 등지방두께 21 mm까지 증가하였고(p<0.05), 22 mm 이상부터 감소하였다. 암소 및 거세우의 평균 성숙도는 각각 6.11과 2.12로 암소의 골화 정도가 높았으며 평균 육색은 4.85 및 4.67 로써 비슷한 수준이었다. 암소의 평균 육질등급은 1등급과 2등급의 중간 수준(평균 3.55)이었고 거세우는 1등급과 $1^+$등급의 중간 수준(평균 2.58)으로 등지방두께가 21 mm까지 육질등급은 향상되었으나(p<0.05), 22 mm 이상부터 암소는 2등급 수준으로 거세우는 1등급 수준으로 낮아졌다. 암소도체 kg당 경락가격은 등지방두께 13-15 mm 구간에서 14,970원으로, 거세우는 등지방두께 10-12 mm 구간에서 17,318원으로 가장 높았다(p<0.05). 도체 kg당 경락가격은 암소는 등지방두께 18 mm이상, 거세우는 15 mm이상에서 유의적으로(p<0.05) 감소되었다. 특히, 거세우의 경우 도체 경락가격은 등지방 두께 15 mm 이상에서 유의적인 차이가 없었다. $1^{++}$등급 출현율은 암소는 등지방두께 19-21 mm 구간에서 7.33%, 거세우는 22-24 mm구간에서 25.04%로 가장 높았다. 육량 A등급은 등지방두께 4-6 mm구간에서 암소 및 거세우 각각 55.67%와 93.33%로 가장 높았다. 암소 및 거세우의 등지방 두께는 육량지수와 각각 $-0.97^{**}$ 및 $-0.96^{**}$로써 상관관계가 높았으며 근내지방도와 각각 $0.20^{**}$ 및 $0.14^{**}$ 상관관계가 있었고 육질 등급($1^{++}$등급 = 1, 3등급 = 5)과의 상관관계는 $-0.15^{**}$로 같았다. 본 연구에서 한우도체의 등지방두께는 도체등급 요인 및 도체가격에 영향을 미치는 것으로 분석되어 금후 한우도체의 등지방 감소를 위한 다양한 연구가 필요하다고 사료된다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제28권7호
• /
• pp.926-935
• /
• 2015

The efficiency of genome-wide association analysis (GWAS) depends on power of detection for quantitative trait loci (QTL) and precision for QTL mapping. In this study, three different strategies for GWAS were applied to detect QTL for carcass quality traits in the Korean cattle, Hanwoo; a linkage disequilibrium single locus regression method (LDRM), a combined linkage and linkage disequilibrium analysis (LDLA) and a $BayesC{\pi}$ approach. The phenotypes of 486 steers were collected for weaning weight (WWT), yearling weight (YWT), carcass weight (CWT), backfat thickness (BFT), longissimus dorsi muscle area, and marbling score (Marb). Also the genotype data for the steers and their sires were scored with the Illumina bovine 50K single nucleotide polymorphism (SNP) chips. For the two former GWAS methods, threshold values were set at false discovery rate <0.01 on a chromosome-wide level, while a cut-off threshold value was set in the latter model, such that the top five windows, each of which comprised 10 adjacent SNPs, were chosen with significant variation for the phenotype. Four major additive QTL from these three methods had high concordance found in 64.1 to 64.9Mb for Bos taurus autosome (BTA) 7 for WWT, 24.3 to 25.4Mb for BTA14 for CWT, 0.5 to 1.5Mb for BTA6 for BFT and 26.3 to 33.4Mb for BTA29 for BFT. Several candidate genes (i.e. glutamate receptor, ionotropic, ampa 1 [GRIA1], family with sequence similarity 110, member B [FAM110B], and thymocyte selection-associated high mobility group box [TOX]) may be identified close to these QTL. Our result suggests that the use of different linkage disequilibrium mapping approaches can provide more reliable chromosome regions to further pinpoint DNA makers or causative genes in these regions.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권12호
• /
• pp.1712-1720
• /
• 2012

We investigated the effect of the amount of body condition loss in the dry period and early lactation in 42 high-yielding Holstein-Friesian cows on milk yield and the share of fatty acids in milk fat. Energy reserves were estimated based on the body condition scoring (BCS) and backfat thickness (BFT). Milk yield and milk composition were determined over 305-d lactation. From d 6 to 60 of lactation, the concentrations of 43 fatty acids in milk fat were determined by gas chromatography. Cows were categorized based on body condition loss from the beginning of the dry period to the lowest point of the BCS curve in early lactation into three groups: low condition loss group (L) ${\leq}0.5$ points (n = 14); moderate condition loss group (M) 0.75 to 1.0 points (n = 16) and high condition loss group (H) >1.0 points (n = 12). Cows whose body energy reserves were mobilized at 0.8 BCS and 11 mm BFT, produced 12,987 kg ECM over 305-d lactation, i.e. 1,429 kg ECM more than cows whose BCS and BFT decreased by 0.3 and 5 mm, respectively. In group H, milk yield reached 12,818 kg ECM at body fat reserve mobilization of 1.3 BCS and 17 mm BFT. High mobilization of body fat reserves led to a significant (approx. 5%) increase in the concentrations of monounsaturated fatty acids-MUFA (mostly $C_{18:1}$ cis-9, followed by $C_{18:1}$ trans-11), a significant decrease in the levels of fatty acids adversely affecting human health, and a drop in the content of linoleic acid, arachidonic acid and docosahexaenoic acid in milk fat. In successive weeks of lactation, an improved energy balance contributed to a decrease in the concentrations of unsaturated fatty acids (UFA) and an increase in the conjugated linoleic acid (CLA) content of milk fat.

• Asian-Australasian Journal of Animal Sciences
• /
• 제29권9호
• /
• pp.1215-1221
• /
• 2016

The prediction of carcass composition in Hanwoo steers is very important for value-based marketing, and the improvement of prediction accuracy and precision can be achieved through the analyses of independent variables using a prediction equation with a sufficient dataset. The present study was conducted to develop a prediction equation for Hanwoo carcass composition for which data was collected from 7,907 Hanwoo steers raised at a private farm in Gangwon Province, South Korea, and slaughtered in the period between January 2009 and September 2014. Carcass traits such as carcass weight (CWT), back fat thickness (BFT), eye-muscle area (EMA), and marbling score (MAR) were used as independent variables for the development of a prediction equation for carcass composition, such as retail cut weight and percentage (RC, and %RC, respectively), trimmed fat weight and percentage (FAT, and %FAT, respectively), and separated bone weight and percentage (BONE, and %BONE), and its feasibility for practical use was evaluated using the estimated retail yield percentage (ELP) currently used in Korea. The equations were functions of all the variables, and the significance was estimated via stepwise regression analyses. Further, the model equations were verified by means of the residual standard deviation and the coefficient of determination ($R^2$) between the predicted and observed values. As the results of stepwise analyses, CWT was the most important single variable in the equation for RC and FAT, and BFT was the most important variable for the equation of %RC and %FAT. The precision and accuracy of three variable equation consisting CWT, BFT, and EMA were very similar to those of four variable equation that included all for independent variables (CWT, BFT, EMA, and MAR) in RC and FAT, while the three variable equations provided a more accurate prediction for %RC. Consequently, the three-variable equation might be more appropriate for practical use than the four-variable equation based on its easy and cost-effective measurement. However, a relatively high average difference for the ELP in absolute value implies a revision of the official equation may be required, although the current official equation for predicting RC with three variables is still valid.