• 약학회지
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• 제56권3호
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• pp.145-151
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• 2012

To develop fundamental data of herbal materials with heat treatment, we studied analytical and extraction methods of benzopyrene in black ginseng and validated by HPLC analysis. Benzopyrene was successfully separated in mobile phase of acetonitrile:water (80 : 20) and detection of excitation 294 nm, emission 404 nm. The calibration curve of benzopyrene was linear over the concentration range of 1.17~37.50 ng/ml with correlation coefficient of above 0.999. The limit of detection (LOD) and quantitation (LOQ) of benzopyrene was 0.25 and 0.75 ng/ml, respectively. Hexane extraction method was used as a new extraction method for benzopyren and the efficient of extraction was over 95%. In conclusion, analytical method and extraction method were suitable for the determination of benzopyrene in the black ginseng and could be applied to fundamental study and guideline of herbal materials with heat treatment.

• 약학회지
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• 제54권1호
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• pp.62-66
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• 2010

Benzopyrene is known to be one of the most powerful carcinogens which can build intercalated motif between base pairs in damaged DNA. The dimension of benzopyrene itself is much bigger than any of the DNA bases and thus the question whether the lesion of some base pair by insertion of benzopyrene can happen with or without a dramatic distortion of the helical structure is a highly interesting theme. In this work we used a molecular mechanics simulation using AMBER program package to go into the conformational characteristics. The condition of the insertion process of the benzopyrene motif from minor groove of the starting structure between the base pairs in the internal area of double helix was investigated using the molecular dynamics simulation at elevated temperature.

• 약학회지
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• 제52권1호
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• pp.12-19
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• 2008

The organic acids (formic acid, acetic acid and propionic acid), phenol and benzopyrene in wood vinegar were determine by HPLC. An $Atlantis^{TM}dC_{18}$ column with a acetonitrile: 0.1% phosphoric acid (5: 95, organic acids), acetonitrile: water (10 : 90, phenol), 100% acetonitrile (benzopyrene) as a mobile phase was used. Retention time of acetic acid, formic acid, propionic acid, phenol and benzopyrene was 4.77, 3.73, 9.08, 30.97 and 6.10 min, respectively. The calibration curves of organic acids, phenol, benzopyrene were linear over the concentration range of $30{\sim}500,\;60{\sim}1000$, and $3{\sim}50\;{\mu}g/ml$ with correlation coefficient of above 0.999. The limit of detection (LOD) and limit of quantitation (LOQ) of acetic acid, formic acid, propionic acid, phenol, and benzopyrene was 1.71 and 5.19, 1.11 and 3.35, 4.87 and 14.74, 6.45 and 19.55, 0.08 and $0.24\;{\mu}g/ml$, respectively. The coefficients of variation for intra- and inter-day assay were $0.21{\sim}4.14$ and $0.07{\sim}1.19%$, respectively and the precision was $95{\sim}115%$. From this study, we suggest that HPLC is suitable method for the determination of organics in wood vinegar and could be applied to quality control of wood vinegar.

• 약학회지
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• 제54권1호
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• pp.67-74
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• 2010

Some of the benzopyrene (BP)-DNA adduct are known to build intercalated motif between flanking base pairs in damaged DNA depending on the structural condition. The size of benzopyrene itself is definitely not comparable with any of the DNA bases and thus the question whether the lesion of some base pair by insertion of benzopyrene can happen with or without a dramatic distortion of the helical structure is a highly interesting theme. In this work we used a molecular dynamics simulation based on the theory of molecular mechanics. The specific consequences about the structural properties of the intercalated structures and benzopyrene motif in minor groove of the double helix are deduced after 5 ns simulation time.

• 한국식품과학회지
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• 제28권4호
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• pp.796-799
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• 1996

분리된 균주와 표준균주의 Bifidobacterium 21종류에 대하여 Salmonella typhimurium TA 98균주를 이용한 in vitro 항돌연변이능을 조사하였다. 실험에 이용된 돌연변이원으로는 Trp-P-1 (3-amino-1,4-dimethyl-$^{5}H-pyrido$ (4, 3-b) indole), benzopyrene, IQ (2-amino-3-methylimidazo [4,5-f] quinoline)와 NQO (4-nitroquinoline oxide) 등이었다. 동결건조된 균체들은 평균적으로 각각 Trp-P-1, benzopyrene, NQO, IQ에 대하여 64, 38, 29, 20%의 항돌연변이능을 나타냈다. Trp-P-1, benzopyrene, IQ에 대하여는 균주의 종류와 성장시기에 따른 항돌연변이능에 큰 차이는 없는 것으로 나타났다. NQO에 대하여는 12시간 배양세포가 5일 배양세포보다 항돌연변이능이 우수한 것으로 나타났다. 본 연구결과는 Bifidobacterium 균주들이 일반적으로 몇 종류의 잘 알려진 돌연변이원에 대한 항돌연변이능을 보유하고 있음을 보여주었다.

• Tuberculosis and Respiratory Diseases
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• 제62권3호
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• pp.197-202
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• 2007

흡연물질로서 benzopyrene, nicotine 및 cotinine등은 기관지상피세포인 Beas2B에서 CuZnSOD, thioredoxin, glutathione reductase 등의 발현량에 영향을 주며 특히 thioredoxin 및 glutathione reductase의 발현량을 노출 후 30분에서 4시간 경과 시간대에 증가시켰다가 24시간 이후에는 억제하는 특징을 나타내었다. 상기한 흡연물질에 의한 항산화효소의 조절기전에는 전사인자인 NNF-${\kappa}B$가 관여하는 것으로 추정되었다.

• 분석과학
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• 제20권2호
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• pp.170-175
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• 2007

올리브유중 벤조피렌을 분석하기 위하여 고속액체크로마토그래피/형광검출기를 이용하였다. 시료의 지방성분을 헥산으로 제거한 후 N,N-DMF 수용액을 다시 헥산으로 추출하여 후로리실 SPE 카트리지로 정제한 후 기기분석하였다. 이동상으로는 아세토니트릴과 물의 혼합용액(8:2)을 사용하였으며 형광검출기의 여기파장은 294 nm이었고 형광파장은 404 nm이었다. 평균 회수율은 95%이었으며, 정량한계는 $0.9{\mu}g/kg$이었다. 대상 식품인 올리브유 중 벤조피렌의 검출수준은 불검출~$1.9\mu}g/kg$이었으나, 식품공전의 기준인 올리브유중 벤조피렌의 최대수준 $2.0{\mu}g/kg$이하이었다.

• 식품산업과 영양
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• 제19권1호
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• pp.13-14
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• 2014

• 식품산업과 영양
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• 제18권2호
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• pp.33-35
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• 2013

• 한국식품위생안전성학회지
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• 제23권1호
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• pp.26-30
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• 2008

다환방향족탄화수소는 환경오염이나 식품의 제조공정 과정 중에 생성될 수 있으며, 홍삼은 수증기로 찌고 건조하여 만들어진다. 시료를 핵산으로 추출한 후 물로 세척하고 후로리실 SPE 카트리지로 정제한 후 고속액체크로마토그래피/형광검출기로 분석하였다. 이동상으로는 아세토니트릴과 물의 혼합용액(8:2)을 사용하였으며 형광검출기의 여기파장은 294 nm이었고 형광파장은 404 nm이었다. 평균 회수율은 105%이었으며, 상대표준편차는 0.5이었다. 대상 식품인 홍삼음료 중 벤조피렌은 검출되지 않았다.