• Journal of Microbiology and Biotechnology
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• 제18권6호
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• pp.1109-1114
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• 2008

To understand the role of intestinal microflora in the biological effect of functional herbs, which have been used in Korea, Japan, and China as traditional medicines, and suggest new bioactive compounds transformed from herbal constituents, the metabolic activities of the functional herb components (ginsenoside Rb1, crocin, amygdalin, geniposide, puerarin, ginsenoside Re, poncirin, hesperidin, glycyrrhizin, and baicalin) toward their bioactive compounds (compound K, crocetin, benzaldehyde, genipin, daidzein, ginsenoside Rh1, ponciretin, hesperetin, 18b-glycyrrhetic acid, and baicalein) were measured in fecal specimens. The metabolic activities of these components were $882.7{\pm}814.5$, $3,938.1{\pm}2,700.8$, $2,375.5{\pm}913.7$, $1,179.4{\pm}795.7$, $24.6{\pm}10.5$, $11.4{\pm}10.8$, $578.8{\pm}206.1$, $1,150.0{\pm}266.1$, $47.3{\pm}58.6$, and $12,253.0{\pm}6,527.6\;{\mu}mol/h/g$, respectively. No differences were found in the metabolic activities of the tested components between males and females, although these metabolic activities between individuals are extensively different. The metabolites of functional herb components showed more potent cytotoxicity against tumor cells than nonmetabolites. These findings suggest that intestinal microflora may activate the pharmacological effect of herbal food and medicines and must be the biocatalytic converter for the transformation of herbal components to bioactive compounds.

• Environmental Analysis Health and Toxicology
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• 제25권1호
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• pp.57-68
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• 2010

This study assessed the lifetime cancer and non-cancer risk of aldehydes and volatile organic compounds exposure of young children at child-care facilities and indoor playgrounds in Korea. The samples were collected at various children's facilities (40 day-care houses, 42 child-care centers, 44 kindergartens, and 42 indoor playgrounds) in summer (Jul~Sep, 2007), winter (Jan~Feb, 2008) and Spring (Mar~Apr, 2008) periods, and analyzed by GC-MS. We estimated the lifetime excess cancer risks (ECRs) of formaldehyde, acetaldehyde, benzene, chloroform, CCI4 and tetrachloroethylene, and the hazard quotients (HQs) of Non-carcinogens (toluene, xylene, benzaldehyde and formaldehyde). Formaldehyde evaluated both cancer and non-cancer risk. The average ECRs of formaldehyde for young children were $1\times10^{-4}{\sim}1\times10^{-5}$ level in all facilities. HQs of four non-carcinogens did not exceed 1.0 for all subjects in all facilities.

• Journal of Applied Biological Chemistry
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• 제57권1호
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• pp.41-45
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• 2014

In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorganism to biotransform protopanaxdiol-type ginsenosides to their less glucosidic metabolites. Other biotransformed metabolites during fermentation were also analyzed using a GC-MS and identified as 2-methyl-benzaldehyde, 4-vinyl-2-methylphenol, palmitic acid, and linoleic acid. Antiulcerogenic activity of the fermented red ginseng extract (FRGE) on gastric mucosal damage induced by 0.15 M HCl in ethanol in rats was evaluated. FRGE was shown to have a potent protective effect on gastritis with 60.5% of inhibition rate at the dose of 40 mg/kg when compared to 54.5% of the inhibition rate at the same dose for stillen, the currently used medicine for treating gastritis. Linoleic acid showed a strong inhibition on gastritis with 79.3% of inhibition rate at the dose of 40.0 mg/kg. FRGE exhibited a distinct anticancer activity including growth inhibition of the two human colon cancer cells HT29 and HCT116. HT29 cells were less susceptible to FRGE in comparison with HCT116 cells. Taken together, fungal fermentation of the red ginseng extract induced hydrolysis of some ginsenosides and FRGE exhibited potent antiulcerogenic and anticancer activities. These results refer to use FRGE as a new source for treating human diseases.

• 한국대기환경학회지
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• 제24권2호
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• pp.206-219
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• 2008

The purpose of this study is to evaluate concentrations of carbonyl compounds during summer season in Seoul metropolitan area. The air samples were collected at 7 sites in this area from June 2001 to June 2003. The carbonyl compounds were analyzed by DNPH/HPLC method. The analytical method applied in this study showed good repeatability, linearity, and sensitivity. The most abundant carbonyl was formaldehyde (average 4.48 ppb), and followed by acetone, acetaldehyde, methyl ethyl ketone, butyraldehyde, propionaldehyde and benzaldehyde, respectively. Concentrations of carbonyl compounds in June were higher than those in August. There was not only higher solar radiation but also higher ozone concentration in June than in August. As a result o photochemical reactions, carbonyl compounds from both primary and secondary sources are likely to contribute to the formation of ozone. The contributions to photochemical ozone creation of two carbonyl compounds such as formaldehyde and acetaldehyde were estimated to be about 70%. Ratios of formaldehyde to acetaldehyde in this study ranged from 1.13 to 4.26, which are generally equivalent levels to those of other urban areas in domestic and foreign countries.

• 한국수산과학회지
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• 제48권5호
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• pp.668-673
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• 2015

The pacific oyster Crassostrea gigas has a desirable taste and flavor that differs from those of other fish and shellfish. In order to develop a high value-added product from individually quick-frozen oyster extract (IQFOE), we prepared an oyster sauce from IQFOE and characterized its volatile compounds using vacuum simultaneous steam distillationsolvent extraction / gas chromatography / mass spectrometry. The moisture, crude protein, crude ash, salinity, pH and volatile basic nitrogen contents of the oyster sauce were 60.6%, 8.2%, 9.2%, 9.3%, 5.7 and 21.0 mg/100 g, respectively. Seventy-six volatile compounds were detected in the cooked odor of the oyster sauce. These volatile compounds included 14 esters, including ethyl acetate, 13 nitrogen- containing compounds, including 2,4,6-trimethyl pyridine, 13 acids, including hexadecanoic acid, 12 alcohols, including ethyl alcohol and 6-methyl heptanol, 6 alkanes, 5 aldehydes, including benzaldehyde, 5 ketones, including 1-(2-furanyl)-ethanone, 4 furans, including 2-furancarboxaldehyde and 2-furanmethanol, 3 aromatic compounds, including d-limonene, and 1 miscellaneous compound. Esters, acids and nitrogen-containing compounds, and alcohols were the most abundant compounds in the odor of the cooked oyster sauce, with some aldehydes, ketones, and furans.

• Journal of the Korean Wood Science and Technology
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• 제35권5호
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• pp.24-37
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• 2007

The Korean Ministry of Environment started controlling indoor air quality (IAQ) in 2004 through the introduction of a law regulating the use of pollutant emitting building materials. The use of materials with formaldehyde emission levels above $1.25 mg/m^2{\cdot}h$ (JIS A 1901, small chamber method) has been prohibited. This level is equivalent to the $E_2$ grade ($>5.0mg/{\ell}$) of the desiccator method (JIS A 1460). However, the $20{\ell}$ small chamber method requires a 7-day test time to obtain the formaldehyde and volatile organic compound (VOC) emission results from solid building interior materials. As a approach to significantly reduce the test time, the field and laboratory emission cell (FLEC) has been proposed in Europe with a total test time less than one hour. This paper assesses the reproducibility of testing formaldehyde and TVOC emissions from wood-based composites such as medium density fiberboard (MDF), laminate flooring, and engineered flooring using three methods: desiccator, perforator and FLEC. According to the desiccator and perforator standards, the formaldehyde emission level of each flooring was ${\le}E_1$ grade. The formaldehyde emission of MDF was $3.48 mg/{\ell}$ by the desiccator method and 8.57 g/100 g by the perforator method. To determine the formaldehyde emission, the peak areas of each wood-based composite were calculated from aldehyde chromatograms obtained using the FLEC method. Formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde and benzaldehyde were detected as aldehyde compounds. The experimental results indicated that MDF emitted chloroform, benzene, trichloroethylene, toluene, ethylbenzene, m,p-xy-lene, styrene, and o-xylene. MDF emitted significantly greater amounts of VOCs than the floorings did.

• 대한화학회지
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• 제9권1호
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• pp.1-7
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• 1965

토루엔, 디메틸폴움아마이드, 디메틸슬포옥사이드, 테트라하이드로퓨란, 아세토나이트라일 및 디옥센 等 溶媒存在下에서 모노클로로醋酸과 亞鉛을 反應시켜 본 結果, 溶媒효果를 나타내었다. 反應試藥의 反應度가 溶媒의 極性 및 親水性에 따라 增加하였다. 같은 溶媒系를 使用하여 에틸모노클로로醋酸을 反應시켜 본 結果, 溶媒효果를 나타냈으나 酸의 境遇보다 그 差가 크지 못하였다. 酸, 에스타, 亞鉛 및 카보닐化合物(벤즈알데히드 및 4-헤프타논)의 反應에 있어서는 알데하이드의 境遇에 있어서는 Reformatsky 反應生成物을 주었으나, 4-헤프타논과의 反應度는 없었다. 反應生成物의 收率은 試藥添加方法에 따라 變化되었다. 最高收率은 酸의 하이드로옥시酸(38.5%), 0.8g의 salt(아세트나이트라일溶媒)이며, 에스타의 境遇에는 에틸신나메이트(19.3%), 폴리머(21.6%)이였다. 카보닐化合物의 境遇에 있어서는 反應溫度에 따라 試藥의 反應度의 變化가 있었다. 硏究結果를 溶媒效果에 關聯시켜 論議하였으며, 硏究方法에 關하여 記述하였다.

• 한국식품과학회지
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• 제45권6호
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• pp.675-681
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• 2013

담양군 죽순으로 제조한 죽순식초와 시판 과실식초의 이화학적 특성인 산도, 무기질, 아미노산, 플라보놀을 비교 분석하고 SBSE-GC/MS방법을 사용하여 향기성분을 분석하였다. 죽순식초의 산도는 4.49%로 제일 낮았으며 무기질 중 Na의 경우 8.36 mg/100 g으로 다른 과실식초에 비해 그 함량이 낮았다. 아미노산은 다른 시판 식초에 비해 tyrosine과 lysine의 함량이 높았으며 flavonol aglycone 중 quercetin이 3.29 mg/L 들어있었다. SBSE방법으로 향기성분을 분석한 결과 aldehyde류의 함량이 시판식초에 비해 높았으며 특이적으로 수면 유도물질로 알려진 oleamide가 처음으로 검출되었다.

• 한국수산과학회지
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• 제22권5호
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• pp.370-374
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• 1989

오징어 가공중의 향기성분의 변화를 알아보기 위하여 Nikerson형 연속추출장치(SDG)를 이용하여 시료의 향기성분을 추출한 후 중성, phenol성, 염기성 및 산성획분으로 나누어 중성 및 염기성획분을 GC 및 GC-MS로 분석$\cdot$동정한 결과 중성획분에서 31성분, 염기성 획분에서 7성분 분석$\cdot$동정되었다. 오징어 가열시료에서 3-methylthiophene, 2-methyl-2-hexanethiol 등의 함황화합물이 검출되었고 탄소수 5인 1-ponton-3-ol, 3-ponton-2-ol, 4-me-thyl-3-pentanal 및 탄소수 3인 3-methyl-1,2-propan-diol, 1-hydroxy-2-propanone과 hexanal, benzaldehy-de를 비롯한 탄소수가 8과 9인 alcohol, ketone 등이 검출되었다. 염기성획분에서는 trimethylamine을 비롯한 6종의 pyrazine류가 분리$\cdot$동정되었으며 2,5-dimethyl pyrazine, 2-ethyl-6-methyl pyrazine, 2,3,5-trimethyl pyrazine 등과 특히 2-ethyl-3,5-dimethyl pyrazine의 함량이 많아 염기성획분의 주된 향기성분으로 나타났다.

• 한국염색가공학회지
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• 제11권1호
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• pp.16-24
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• 1999

The effects of two-step fixation of steaming and baking on the dischargeability of cotton fabrics dyed with C.I. Reactive Black 5(Bl-5) were investigated when the concentrations of $K_2CO_3$ and benzaldehyde sodium bisulfite(BASB) were increased over 120/kg. Remarkably increased dischargeability resulted from baking for 3 min or more at 160t after steaming for 8 min or more at $102^\circ{C}$, but 120g/kg or more amounts of $K_2CO_3$ and BASB(50%) had little influence on dischargeability. Therefore the discharge mechanism can be suggested that covalent bonds between cellulose and Bl-5 undergo $S_N2$ attack by hydroxide ion formed by the reaction of $K_2CO_3$ and water in steaming at $102^\circ{C}$ first and then, through transition states they are cleavaged in baking at 160t to yield hydrolyzed Bl-S and compounds of BASB and Bl-5 isolated from fiber, which are undyeable and removed by washing. The effect of urea, one of the hydrotrope agents, on discharge printing was also studied. The result which dischargeability was greatly improved by increasing the steaming time from 8 min to 15 min at $102^\circ{C}$ or by increasing the amount of urea obviously shows that water in steaming and urea in print paste play an important role in discharge printing. And as an increase of the baking time from 5 min to 7 min at $160^\circ{C}$ makes it possible to improve dischargeability, it is once more confirmed that high temperature of about 160t is exactly required to discharge the dyed Bl-5. The colored discharge printing demands a more amount of urea because urea contributes to the putting color fixation as well as the discharge reaction.