• Title/Summary/Keyword: behavior of virus

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Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus

  • Lee, Joo Hyun;Choi, Jae Young;Tao, Xue Ying;Kim, Jae Su;Kim, Woojin;Je, Yeon Ho
    • The Plant Pathology Journal
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    • v.29 no.3
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    • pp.330-337
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    • 2013
  • Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction.

Molecular biological characterization of the new virus isolated from abnormally swimming salmonid - Reverse transcriptase activity - (이상유영(異常遊泳) 증상(症狀)을 나타내는 연어과 어류(魚類)에서 분리(分離)된 새로운 바이러스의 분자생물학적(分子生物學的) 특성(特性) - 역전사효소 활성 -)

  • Oh, Myung-Joo
    • Journal of fish pathology
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    • v.9 no.2
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    • pp.177-183
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    • 1996
  • A new RNA virus isolated from abnormally swimming behavior has caused mortalities in salmonid fish (Oh et al., 1995 a), A reverse transcriptase (RTase) activity of the virus was determined by using poly r(A) : oligo d(T) as templete : primer. This RTase activity was associated with virus particles of buoyant density of 1.16 g/ml. The virus particles in sucrose fractions were enveloped and were about 85 nm diameter with central electron-dense core. The brain and kidney samples of artificially infected fish showed RTase activity. Virus particle associated proteins about 120, 80, 65, 61, 48, 42, 35, 30, 25, 19, and 15 kDa were observed when examined by polyacrylamide gel electrophoresis analysis. This study showed the presence of a new retrovirus in salmonid fish, which tentatively called RVS (Retrovirus of salmonid).

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The Detection System for Hosts infected Malware through Behavior information of NAC post-connect (NAC 의 post-connect에서 행위정보를 사용한 악성코드 감염 호스트 탐지 시스템)

  • Han, Myung-Mook;Sun, Jong-Hyun
    • The Journal of Korean Association of Computer Education
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    • v.13 no.6
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    • pp.91-98
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    • 2010
  • NAC(Network Access Control) has been developed as a solution for the security of end-point user, to be a target computer of worm attack which does not use security patch of OS and install Anti-Virus, which spreads the viruses in the Intra-net. Currently the NAC products in market have a sufficient technology of pre-connect, but insufficient one of post-connect which detects the threats after the connect through regular authentication. Therefore NAC users have been suffered from Zero-day attacks and malware infection. In this paper, to solve the problems in the post-connect step we generate the normal behavior profiles using the traffic information of each host, host information through agent, information of open port and network configuration modification through network scanner addition to authentication of host and inspection of policy violation used before. Based on these we propose the system to detect the hosts infected malware.

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Factor Associated with the Intention of Human Papillomavirus Vaccination with Nursing Students: Based on the Theory of Planned Behavior (간호대학생의 인유두종바이러스 접종 의도 및 영향요인: 계획된 행동이론을 기반으로)

  • Lee, Yoon-Hee;Park, Keum-Ok
    • Journal of the Korea Convergence Society
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    • v.12 no.9
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    • pp.343-350
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    • 2021
  • The purpose of this study was to apply Ajzen's theory of planned behavior to identify factors that affect nursing students decisions to receive Human Papilloma Virus(HPV) vaccination. Data collection was done using self-report questionnaires with 195 nursing students in D city. Data were analyzed using percentage, mean, standard deviation, t-test, Pearson correlation analysis and hierarchial regression with the SPSS Win 25.0 Program. The mean score of intention to receive HPV vaccine was 5.24±1.66 out of a possible 7. In the hierarchial regression analysis, attitudes (β=.446, p<.001), perceived behavior control (β=.321, p<.001) subjective norm (β=.132, p=.041), to receive HPV vaccine were significant predictors and explained 54.4% of intention to receive HPV vaccine. it is suggested that promotion programs and social environment to encourage to think it positively and perceive it easy and convenient should be developed, with nursing students' educatinon for sex education and public relations activitiesas as a nursing educators.

Hyperkinetic Rat Model Induced by Optogenetic Parafascicular Nucleus Stimulation

  • Moonyoung Chung;Young Seok Park
    • Journal of Korean Neurosurgical Society
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    • v.66 no.2
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    • pp.121-132
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    • 2023
  • Objective : The parafascicular nucleus (PF) plays important roles in controlling the basal ganglia. It is not well known whether the PF affects the development of abnormal involuntary movements (AIMs). This study was aimed to find a role of the PF in development of AIMs using optogenetic methods in an animal model. Methods : Fourteen rats were underwent stereotactic operation, in which they were injected with an adeno-associated virus with channelrhodopsin (AAV2-hSyn-ChR2-mCherry) to the lateral one third of the PF. Behavior test was performed with and without optical stimulation 14 days after the injection of the virus. AIM of rat was examined using AIM score. After the behavior test, rat's brain was carefully extracted and the section was examined using a fluorescence microscope to confirm transfection of the PF. Results : Of the 14 rats, seven rats displayed evident involuntary abnormal movements. AIM scores were increased significantly after the stimulation compared to those at baseline. In rats with AIMs, mCherry expression was prominent in the PF, while the rats without AIM lacked with the mCherry expression. Conclusion : AIMs could be reversibly induced by stimulating the PF through an optogenetic method.

Phenobarbital and zonisamide treatment of a cat with epilepsy of unknown cause

  • Lee, Ki-Ho;Park, Jun-Seok;Kim, Jung-Kook;Seo, Kyoung-Won;Song, Kun-Ho
    • Korean Journal of Veterinary Service
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    • v.40 no.2
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    • pp.143-147
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    • 2017
  • A Korean domestic short hair (1-year-old, male) presented with 2 to 3 weeks of seizures, aggressive behavior, vomiting, anorexia, and lethargy. The frequency of seizure had gradually increased from once a week to once every 3 hours. Physical and neurologic examination, diagnostic screening tests, including complete blood count (CBC), serum chemistry, electrolyte, coagulation test, X-ray, ultrasonography, and urinalysis were performed. Feline Leukemia Virus (FeLV), Feline Immunodeficiency Virus (FIV) and Toxoplasma spp. All tested negative, but the Feline Corona Virus (FCoV) kit revealed a positive result. To determine the exact diagnosis, magnetic resonance imaging (MRI) was performed but yielded no specific findings. The patient was then diagnosed with idiopathic epilepsy and treatment of phenobarbital was initiated. A month's treatment with phenobarbital proved ineffective as symptoms worsened. Zonisamide was then selected as an additional anticonvulsant. After adding zonisamide, symptoms improved, and seizures abated for 15 months. This is the first case report in South Korea describing the use of phenobarbital and zonisamide in the treatment of a cat with idiopathic epilepsy.

Factors Predicting HPV Vaccination Practices among Female College Students (여대생의 인유두종바이러스 예방접종실천 예측요인)

  • Kim, Sun-Hee
    • Korean Parent-Child Health Journal
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    • v.20 no.1
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    • pp.39-47
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    • 2017
  • Purpose: This study was conducted to investigate the factors predicting HPV (Human Papilloma Virus) vaccination practices among female college students. Methods: A convenience sample of 207 female students attending four universities in one metropolitan city participated. Self-report questionnaires consisted of general characteristics, characteristics related prevention of cervical cancer, knowledge of HPV, knowledge of cervical cancer vaccination, and health beliefs related to HPV vaccination. Data were analyzed by $x^2$ test, independent t-test, and bivariate logistic regression. Results: Factors predicting HPV vaccination practices were information about HPV (OR=3.37), experience of HPV test (OR=12.71), and health beliefs related to HPV vaccination (OR=1.13). Conclusion: In order to increase the practice rate of HPV vaccination, it is necessary to provide simple key information that is easy to understand, rather than expert knowledge about HPV. Therefore, it is necessary to provide a way for college students to get information about virus easily. It is necessary to intervene integrally with the facilitation factor and obstacle factor of vaccination practice.

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The Etiologic Roles and Carcinogenic Mechanisms of Human Papilloma Virus in Head and Neck Squamous Cell Carcinoma (두경부 편평세포암종의 발암 원인으로 인간유두종 바이러스(Human Papilloma Virus)의 역할 및 이와 관련된 발암 기전에 관한 연구)

  • Shin, Dong-Hyun;Lee, Sei-Young;Koo, Bon-Seok;Kim, Se-Heon
    • Korean Journal of Head & Neck Oncology
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    • v.25 no.1
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    • pp.28-32
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    • 2009
  • Background : The most frequently reported risk factors for head and neck suamous cell carcinoma are smoking and alcohol. But in a recent overview, human papilloma virus(HPV) infection was revealed the important carcinogenic factor in oropharyngeal cancer. We aimed to clarify whether HPV directly effects on the oncogenesis and biologic behavior of hean and neck squamous cell carcinoma by comparison with infection prevalence, and physical status of virus. Material and Method : We used HPV genotyping DNA chip(Biocore, Korea, Seoul) arrayed by multiple oligonucleotide probes of L1 sequence of 26 types of HPV and HPV genotypes are identified by fluorescence scanner. The copy numbers of HPV E2 and E6 open reading frames(ORF) were assessed using a TaqMan-based 5'-exonuclease quantitative real-time PCR assay. The ratio of E2 to E6 copy numbers was calculated to determine the physical status of HPV-16 viral gene. Results : We observed a significant difference in HPV prevalence between tonsillar cancer group and control group(73.1% vs. 11.6%), and most of the HPVs were type 16(87.2%) and integrated(94.1%) state. In terms of oral tongue cancer, we demonstrate that 30.5% has integrated HPV-16 in cancer tissue. But Glottic cancer only 1% is related to HPV-16 integration. Conclusion : This study revealed significant relationship of HPV prevalence with oropharyngeal and oral tongue squamous cell carcinoma. Most of HPV were 16 type and integrated or mixed, HPV-16 integration could be directly related to the carcinogenesis.

Knowledge and Attitudes Not Strongly Related to COVID-19 Prevention, Behaviors, and Vaccination Among Filipino College Students

  • De Castro, Gefilloyd L.;Eijansantos, Abee M.;Jalani, Nuriza P.;Amora, Joni Czarina P.;Lantaya, Gualberto A. Jr.
    • Asian Journal for Public Opinion Research
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    • v.10 no.4
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    • pp.316-339
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    • 2022
  • The effects of the COVID-19 pandemic across various sectors are far-reaching, and the education sector is not an exemption. Approved and recognized vaccines are clinically proven to provide protection and have been considered one of the primary mitigations against the virus. Knowledge, attitude, and related practices (henceforth KAP) toward the virus is widely considered to be an essential cognitive key in public health for health intervention, prevention, and promotion. Previous studies revealed a positive correlation between knowledge and attitude. This study investigated the KAP and their relationship among the 857 college students in one state college in the Philippines using an online survey. Using descriptive statistics, the findings showed that the majority of the respondents have been fully vaccinated against COVID-19. Respondents were shown to be knowledgeable about COVID-19, specifically about its transmission, clinical symptoms, treatment, risk groups, isolation, prevention, and control. Positive attitudes toward individual and governmental mitigation and control of the virus were shown. Their scores on practices revealed that they always exhibit protective and preventive behavioral practices. Pearson correlation revealed a weak significant relationship between knowledge and practice, and a fair significant relationship between attitude and practice. No significant relationship is found between knowledge and attitude. This study hopes to find its place in the literature worthy of consideration as empirical support toward understanding a specific group's perceptive risk and preventive behavior toward the virus, policy making, and health intervention and promotion.

The Study of Heuristic Scanning Technique and Behavior Blocking Technique to Detect Virus (바이러스 탐지를 위한 휴리스틱 스캐닝 기법 및 행위 제한 기법 분석)

  • 김은영;오형근;배병철
    • Proceedings of the Korean Information Science Society Conference
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    • 2002.10c
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    • pp.577-579
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    • 2002
  • 급속도로 증가하고 있는 개인 단말 사용자와 네트워크의 광역화로 악성코드의 일종인 바이러스의 출현으로 그 해당 피해가 급증하고 있다. 이러한 현실에서 이전의 시그너쳐 기반 스캐닝 기법은 알려지지 않은 바이러스 및 신종 바이러스를 신속히 탐지할 수 없으므로 탐지성능이 급감하고 있다. 따라서 이전의 시그너쳐 기반의 스캐닝 기법의 단점을 보완하면서 새로운 기법의 바이러스를 탐지하기 위한 기법으로 제안된 휴리스틱 스캐닝 기법 및 행위 제한 기법에 대해 기술하겠다.

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