• Animal Bioscience
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• 제34권4호
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• pp.516-524
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• 2021

Objective: The genetic evaluation of Santa Inês sheep was performed for resistance to gastrointestinal nematode infection (RGNI) and body size using different relationship matrices to assess the efficiency of including genomic information in the analyses. Methods: There were 1,637 animals in the pedigree and 500, 980, and 980 records of RGNI, thoracic depth (TD), and rump height (RH), respectively. The genomic data consisted of 42,748 SNPs and 388 samples genotyped with the OvineSNP50 BeadChip. The (co)variance components were estimated in single- and multi-trait analyses using the numerator relationship matrix (A) and the hybrid matrix H, which blends A with the genomic relationship matrix (G). The BLUP and single-step genomic BLUP methods were used. The accuracies of estimated breeding values and Spearman rank correlation were also used to assess the feasibility of incorporating genomic information in the analyses. Results: The heritability estimates ranged from 0.11±0.07, for TD (in single-trait analysis using the A matrix), to 0.38±0.08, for RH (using the H matrix in multi-trait analysis). The estimates of genetic correlation ranged from -0.65±0.31 to 0.59±0.19, using A, and from -0.42±0.30 to 0.57±0.16 using H. The gains in accuracy of estimated breeding values ranged from 2.22% to 75.00% with the inclusion of genomic information in the analyses. Conclusion: The inclusion of genomic information will benefit the direct selection for the traits in this study, especially RGNI and TD. More information is necessary to improve the understanding on the genetic relationship between resistance to nematode infection and body size in Santa Inês sheep. The genetic evaluation for the evaluated traits was more efficient when genomic information was included in the analyses.

• Journal of Microbiology and Biotechnology
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• 제18권10호
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• pp.1689-1694
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• 2008

The ability to detect Salmonella spp. is essential in the prevention of foodborne illness. This study examined a Salmonella spp. detection method involving the application of immunomagnetic separation and immunoliposomes (IMS/IL) encapsulating sulforhodamine B (SRB), a fluorescent dye. A quantitative assay was conducted by measuring the fluorescence intensity of SRB that was produced from an immunomagnetic bead-Salmonella spp.-immunoliposome complex. The results indicated detection limits of $2.7{\times}10^{5}$ and $5.2{\times}10^{3}$ CFU/ml for Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterka subsp. enterka serovar Typhimurium (S. Typhimurium), respectivley. The signal/noise ratio was improved by using 4% skim milk as a wash solution rather than 2% BSA. In addition, higher fluorescence intensity was obtained by increasing the liposome size. Compared with the conventional plating method, which takes 3-4 days for the isolation and identification of Salmonella spp., the total assay time of to h only including 6 h of culture enrichment was necessary for the Salmonella detection by IMS/IL. These results indicate that the IMS/ IL has great potential as an alternative rapid method for Salmonella detection.

• Journal of Welding and Joining
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• 제26권1호
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• pp.69-75
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• 2008

Spot welding which use the main process for side block production of stainless steel railway vehicle is legged behind in laser welding about a quality and productivity. Although the laser welding has many potential advantages such as low heat input and aspect ratio of weld bead, its application to a new structural component still is required many engineering data including mechanical properties such as tensile, fatigue strength, etc on. Therefore, experimental analysis was carried out to understand the fatigue phenomena of different thickness stainless steel overlap joining panels by Nd:YAG laser welding. The fatigue life curves were obtained through fatigue tests with the various levels of applied load. The fatigue life is related with the parameters such as gap size and penetration depth through experiment. As the results, tensile strength and fatigue life were proportional in heat input level and gap was identified the major factor for fatigue life. Also we could know that deferent a-ferrite content at HAZ depend on welding heat input was important factor to determine a formation of initial crack and total fatigue life cycle.

• 한국자동차공학회논문집
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• 제13권3호
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• pp.64-73
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• 2005

This paper is concerned with the simulation-based parameter determination for the tool design in the stamping process of the complicated auto-body member. The CAE procedure of the stamping process is proposed so that troubles such as wrinkle, springback and excess metal be eliminated with changing parameters such as the blank size, the restraining force of the draw-bead and the embossing shape in the die face. The selected indicators of failure during forming are wrinkling. the amount of spring after unloading of the tool, the amount of excess metal developed .The proposed analysis scheme is applied to the tool and process parameter design for the front side member of a RV car. The simulation results show that the scheme can produce sound product from the viewpoint of thickness distribution, the contact condition between tools and the blank, the shape accuracy and so on.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.410.1-410.1
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• 2016

Vanadium dioxide ($VO_2$) is a well-known material that exhibits a metal-semiconductor transition at 340 K, with drastic change of transmittance at NIR region. However, $VO_2$ based thermochromics accompany with low visible transmittance value and unfavorable color (brownish yellow). Herein, we demonstrate the adjustment of visible transmittance of $VO_2$ thin film by nanosphere template assisted patterning process using sol-gel method. 2-Dimenstional honeycomb shape was varied as function of diameter of nanosphere and coating conditions. The morphological geometry of the films was investigated by FE-SEM and AFM. Result shows that inversed shape of nanosphere was formed clearly and pattern width was altered according to the bead size. This structure creates the geometrical blank area from the position of nanosphere which improves the optical transmittance at the visible region. Moreover, such patterned $VO_2$ thin film not only maintains the optical switching efficiency, but also generate the gorgeous scattering effect which presumably support the glazing application.

• Journal of Welding and Joining
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• 제32권6호
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• pp.47-55
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• 2014

The purpose of the study is to produce a mechanically improved weld and minimum variation of color through comparing unpulsed and pulsed GTAW (Gas Tungsten Arc Welding) for pure titanium (CP grade7) tube. Pulsed GTAW using 60 A peak current and 20 A background current (1:9) achieved the wider window of welding conditions having part and full penetration without burn-through than the case of unpulsed GTAW. Moreover, the pulsed welding reduced a discoloration on the back bead of the weld and the size of microstructures (basket weave and serrated ${\alpha}$). That is because the pulsed welding has it's a low heat input and severe weld flow induced from electric current variation. Furthermore, the pulsed welding improved the bending property of the welded Ti tube. The enhanced bending property for the pulsed GTAW was due to the insignificant discoloration on the weld surface with maintaining the metal polish.

• 환경위생공학
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• 제20권2호
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• pp.47-55
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• 2005

The World Health Organization(WHO, 1998) and the United States Environmental Protection Agency (USEPA, 1992) recommended $2{\mu}\;guranium/{\ell}$ in drinking water as a guideline. The Korea Institute for Environmental Research recently reported that the radioactive pollution in ground water was almost negligible In Korea$(1999\~2002)$. Cs were cast into beads(2mm in wet form) and treated with hexamethylene diisocyanate for stability in acidic aqueous solution through cross-linking of the beads surfaces. The removal study was carried out in a static batch system and a flow system. In the static system, a certain amount of sample water was confined in a vessel and beads(dry weight 0.5g) were packed into it in order to adsorb uranium for a certain period of time. Afterwards the remaining uranium in water vessel was determined by inductively coupled plasma mass spectrometry. The effective pH range was 4 to 8. The smaller the size of beads, the better the removal efficiency. Furthemore, the lower the flow rates, the higher the removal efficiency. The results showed that chitosan beads can be effectively used for the removal of uranium contained in water.

• Journal of Welding and Joining
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• 제30권2호
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• pp.70-75
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• 2012

In this study, effect of nitrogen volume in the shielding gas of Ar-$N_2$ mixing gas on the bead shape, hardness and microstructure of GTA welds of 3mm thick Commercial Pure Ti was investigated. As the nitrogen volume increased, the welding current for full penetration was reduced and hardness in the fusion zone significantly increased compared with that of the base metal, but there is no difference in the hardness of HAZ. Microstructure in the fusion zone with pure Ar gas changed from equiaxed alpha of the base metal to serrated alpha. On the other hand, microstructure using Ar-$N_2$ mixing gas changed to acicular alpha. With the increasing of nitrogen content, the amount of acicular alpha increased and the size of that was fine.

• 대한기계학회논문집A
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• 제28권9호
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• pp.1429-1434
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• 2004

Immunoassay is one of the important analytical methods for clinical diagnoses and biochemical studies, but needs a long time, troublesome procedures and expensive reagents. In this study, therefore, we propose the micro filter chip with microbeads for immunoassay, which has pillar structures. The advantage of the proposed micro filter chip is to use simple fabrication process and cheap materials. The mold was made by the photolithography technique with Si wafer and negative photoresist SU-8. The replica was made of PDMS, bonded on the pyrex glass. The micro filter chip consists of inlet channel, filter chamber and outlet channel. HBV (Hepatitius B virus) monoclonal antibody (Ag1) labeled with biotin were immobilized onto streptavidin coated beads of 30∼50 $\mu$m size. Fluorescein isothiocyanate (FITC)-labeled HBV monoclonal antibody (Ag8) was used to detect HBsAg (Hebatitis B virus surface Antigen), and fluorescence intensity was monitored by epi-fluorescence microscope. In this study, the immune response of less than 30 min was obtained with with the use of 100 $m\ell$ of sample.

• Journal of Welding and Joining
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• 제20권2호
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• pp.95-101
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• 2002

After high power lasers are avaliable in the commercial market, the number of applications of the laser welding has been increased in manufacturing industries. Although the tailored blank laser welding of butt jointed steel sheets is well known recently in the automotive industries, the lap joint laser welding is a new technology to the automotive manufacturing people as well as the design people. But the deep penetration laser welding seems to be preferred to the partial penetration welding for the lap joint welding in the automotive manufacturers because the partial penetration is a serious deflect for the butt joint. In this study, the feasibility of partial penetration welding fur the lap joint $CO_2$ laser welding was studied fur the 1mm thick 390MPa high strength steel sheets for automotive bodies. The process window of the lap joint partial penetration welding was obtained from experiments with the gap size and the welding speed as process parameters. The partial penetration welding was found excellent on the basis of the tensile shear strength and sectional geometry. The bead width, input energy Per volume, tensile-shear strength, deformation energy and the sectional geometries after tensile-shear tests of partial penetration welded specimens are compared with those of full penetration welded specimens with a series of gaps and welding speeds.