• 미생물학회지
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• 제40권2호
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• pp.115-120
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• 2004

시안세균(Anabaena cylindrica)의 생장을 효과적으로 억제하는 세균을 분리하여 NG-2로 구분하였다. 이 세균은 그람 음성의 막대형 세균으로서 1.3∼$1.8{\times}0.35{\mu}m$ 정도의 크기를 보였으며, 카탈라제와 옥시다제 양성 반응을 나타내었고, 35∼$40^{\circ}C$아 pH 9.0의 적정생장 조건을 보였다. A.cylindrica의 NG-2를 JM 배지에서 혼합배양할 경우 두 미생물군은 서로 반비례적으로 성장하였으며, 24시간 이내에 A. cylindrica의 영양세포가 거의 완전히 소멸하였다. NG-2는 빛이 있는 조건에서만 효과적으로 A. cylindrica를 분해하였는데, 이는 NG-2의 A. cylindrica 분해활성이 숙주의 광합성 작용에 매우 의존적임을 의미한다. 분리세균의 A. cylindlica 분해작용을 현미경으로 관찰한 결과 NG-2는 숙주세포에 부착하지 않고 배지 내에 산재한 상태에서 영양세포를 분해하였으며, 이형세포는 분해되지 않았다. 또한 영양세포의 세포벽이 분해되면 분해세균들이 filament 주위에 집중적으로 부착하는 콜로니를 형성함으로써 A. cylindrica를 완전히 분해하는 것이 확인되었다. A. cylindrica에 대한 NG-2의 분해활성은 인공배지 뿐만 아니라 강물에서도 유사한 결과를 나타내었다. 또한 발포 polystyrene 재질의 bead에 세균을 부착함으로써 A. cylindrica 분해 효율을 더 상승시킬 수 있었다.

• 한국미생물·생명공학회지
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• 제14권5호
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• pp.399-405
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• 1986

무증자 전분의 효소당화시 분쇄마찰매체를 첨가하여 분쇄마찰 효과를 주어 당화를 촉진시키는 새로운 무증자 전분 당화법을 이용하여 HFCS의 제조에 적합한 포도당 함량이 높은 고농도 당액을 얻기 위해 연구하였다. 생전분을 고농도 당화를 위해 22.5, 39, 그리고 45%(w/v)와 같이 농도를 고농도까지 증가시켜 가면서 당화시킨 결과, 분쇄마찰 반응계를 활용할 경우 39%(w/v)와 같은 높은 전분 농도에서도 효율적인 당화가 가능하였으며, 8시간 후 75%, 24시간 후에는 92% 이상이 분해되었고 이때 당 농도는 425g/L 수준에 이르렀다. 초 고농도로 전분을 투입한 경우에도 전분을 batch식으로 투입하지 않고 분할 투입하는 fed-batch식으로 분할 투입한 결과 45%(w/v)와 같은 초 고농도에서도 우수한 결과를 얻었다. 또한 고농도에서는bead size가 큰 것이 당화촉진 효과가 컸다. 생성된 당조성을 HPLC로 분석한 결과 증자법의 당조성과 거의 유사한 glucose 95%, maltose 0.7%, 그리고 higher saccharide 4.5%로써 HFCS 제조에 적합한 특성을 갖추었다. 분쇄매체의 shearing에 의한 효소실활을 검토한 바 본 실험과 같은 교반하에서는 효소가 비교적 안정하였고. 특히 $Ca^{++}$은 효소 안정화에 매우 중요한 역할을 수행하였다.

• Journal of Welding and Joining
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• 제16권4호
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• pp.55-62
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• 1998

While excellent joint quality has been obtained using dry chamber underwater welding methods, the size limitations imposed by this process restrict its use for underwater construction work. The wet underwater shielded metal-arc welding eliminates this restriction but suffers from poor weld properties by the 1-pass bead-on-plate welding due to the excessive diffusible hydrogen. On the other hand, in the wet underwater welding, it is well known that the quantity of diffusible hydrogen in multi-pass welded parts reduce to less than that in 1-pass welded parts. Therefore, in this paper, welding experiments are made the 3-pass bead-on-plate welds by using TMCP and normalized steel plates and E4301 and cellulose coated electrode. After that, The amounts of the hydrogen absorbed into the 3-pass welded area were measured according to the JIS Z 3118 specification. The microstructural changes as well as the microhardness distribution after the underwater 3-pass welding were also investigated using Vickers microhardness tester and S.E.M and O.M. The results indicated that the quantity of diffusible hydrogen in 3-pass welded areas was reduced little less than a half of one of that in 1-pass welded areas at the specific welding condition. As a result, the cold cracking of 3-pass welded areas decreased by reduced effect of diffusible hydrogen. In the underwater 3-pass welding, the micrography of cold cracking fracture surface showed mainly the cleavage of hydrogen embrittlement.

• Journal of Welding and Joining
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• 제17권1호
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• pp.104-115
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• 1999

판재의 비드 용접과정에서 열응력과 각변형의 발생기구 및 크기를 판재 단면에 대한 2차원 유한 요소해석을 통해 규명하고자 할 때 판재의 3차원 특성을 판재 길이의 크기효과로 간주하여, 구속경계조 건으로 설정함으로써 2차원 해석으로도 더욱 실제에 근접한 현상해석이 가능함을 제안하고자 하였다. 먼저 용접 입열에 의한 판재 내부의 천이 온도분포를 해석하였고, 이를 열응력 해석에 활용하였다. 2차 원 열응력 해석에 있어, 용접도중에 단면 전체가 길이 방향으로 동일한 크기의 변위를 한다고 가정하여 일정 변위를 길이 방향 경계조건으로 설정하고, 판재의 길이에 따라 각변형의 발생이 구속된다고 가정 하여, 판재의 길이에 의한 구속효과를 상당 구속력으로 간주하여 이를 단면 부재의 회전방향에 대한 경 계조건으로 설정함으로써 판재의 3차원 특성을 고려하고자 하였다. 제안된 방법에 의한 응력 분포 형태, 각변형 크기 등의 해석 결과가 기존의 2차원 해석 결과에 비해 실제에 더 근접함을 보여 주었다.

• 대한조선학회지
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• 제21권1호
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• pp.43-47
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• 1984

The purpose of this paper is to investigate theoretically the effect of residual stress due to welding in stress intensity factor of a plate containing the Model I Crack in different crack size and location, and on fatigue crack growth rate. The initiation of crack is found to be possible only in the region of tensile residual stress. The most dangerous crack has the values of d/b and a/b equal to about 0.6 and 1.0, respectively, where d/b is the ratio of distance from the crack to welding bead and the width of tensile residual stress region and a/b is the ratio of crack length and tensile residual stress region. The crack perpendicular to and on the line of welding bead and with a/b equal to about 0.6 has maximum stress intensity factor. The theoretical fatigue crack growth rate under residual stress and applied stress, which is obtained from Forman's Law by stress superposition, is relatively in good agreement with Glinka's[8] experimental value. The fatigue crack growth is shown to be retarded due to residual stress distribution.

• 한국레이저가공학회지
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• 제13권4호
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• pp.1-6
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• 2010

Recently the use of ultra high strength steels (UHSS) in structural and safety component is rapidly increasing in the automotive industry. For example, 1.5GPa grade hot stamping with die-quenching of boron steel 22MnB5 could apply crash-resistant parts such as bumpers and pillars. The development of laser welding process of hot stamping steels, fundamental bead-on-plate welding and lap joint welding test were carried out using 3kW Nd:YAG laser. Local hardening & HAZ softening occurred in hot stamping steel as a result of metallurgical change caused by the welding heat input in the Nd:YAG laser welding process. The size of soft zones in the hot stamping steel was related to the welding heat input, being smaller at high speeds which generated a smaller heat input. Also in the case of lap joint design structure, same welded characteristics were shown. The HAZ softening degree was controlled to ensure the joint strength.

• Journal of Welding and Joining
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• 제11권2호
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• pp.74-85
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• 1993

Effect of Si metal powders addition with the plasma transferred arc(PTA) overlaying process on characteristics of the alloyed layer in aluminum alloy(A5083) has been investigated. The overlaying conditions were 175-250A in plasma arc current, 500mm/min in travel speed, the 5-20g/min in powder feeding rate. Main results obtained are summarized as follows. 1)Sufficient size of molten pool on surface of base metal was required for forming an alloyed layer; in a fixed travel, the formation of alloyed layer with clear and beautiful surface depend upon the plasma arc current and powder feeding rate; the greater plasma arc current and the smaller powder feeding rate were, the better bead was formed. Optimum alloyed conditions by which an excellent alloyed bead obtained was 225A in plasma arc current. PTA process made it possible to form an alloyed layer with up to 67wt% Si. 2)Microstructure in the alloyed layer was in accord with prediction from the Al-Si phase diagram 3)The hardness of the alloyed layer increased in proportion to Si content. 4)As volume fraction of primary Si increased, the specific wearness of the alloyed layer was significantly improved. However, no further improvement was found when the volume fraction was greater than about 30%. 5)Utilizing the PTA process, a crack free alloyed layer with maximum hardness of about Hv 310 could be obtained.

• Journal of Welding and Joining
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• 제27권2호
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• pp.44-50
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• 2009

The characteristics of arc pressure, current density and heat flux distribution are important factors in understanding physical arc phenomena, which will have a marked effect on the penetration, size and shape of a weld in TIG welding. The purpose of this study is to find out the effect of the heat flux on the melting efficiency and penetration shape in TIG welding using the results of the previous investigators. The conclusions obtained permit to draw a proper method which derived the heat flux distributions by arc pressure distribution measurements, but previous researchers calculated heat flux and current distribution with the heat intensity measurements by the calorimetry. Heat flux of Ar gas arc was concentrated at the central part and distributed low from the arc axis to the radial direction, that of He mixing arc was lower than that of Ar gas, and it was wide distributed to radial direction. That showed a similar characteristic with the Nestor's by calorimetry calculated values. Throughout heat flux drawn in this study was discussed melting efficiency and penetration shape on Ar gas and He mixing gas arc.

• 대한화학회지
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• 제17권3호
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• pp.198-206
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• 1973

이형제와 유리분말의 혼합물을 회전시킬 수 있는 원형전기로 장치(장치 1), 유리분말을 자유낙하 시킬 수 있는 관형 전기로 장치 (장치 2) 및 유리분말을 propane gas의 화염과 함께 분사시킬수 있는 관형장치 (장치 3)를 사용하여 glass beads를 제조하였다. 사용한 기재는 한국산 sodium silicates glass 1,2, boro silicates glass 및 lead silicates glass 이며 cullet의 크기는 60mesh-300mesh이다. P.G.를 기재로 하여 이들 장치에 의하여 glass beads를 제조할 경우, 장치 1의 최적온도는 880$^{\circ}C$이고 장치 2의 최적온도는 980$^{\circ}C$이며 장치 3의 최적온도는 1100$^{\circ}C$이었다. 그렇지만 처리시간과 응착률 등으로 보아 장치 3의 방법이 가장 효율적이었다.

• Journal of Welding and Joining
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• 제21권3호
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• pp.85-91
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• 2003

Narrow gap welding has many advantages over conventional V-grooved butt welding such as high productivity, small deformation and improved mechanical property of joints. With narrower groove gap, less arc heat input is expected will all the other advantages of narrow gap welding. The main defects of narrow gap welding include the lack of root fusion, convex bead surface and irregular surface, all of which have negative effects on the next welding pass. This paper suggests an up-and-down torch oscillation for ultra narrow gap welding with gap size of 5mm and investigates the proper welding conditions to fulfill the reliable and high welding quality. First, GMA welding model was suggested for ultra narrow gap welding system with Halmoy's model referenced for wire melting modeling. And the arc length in ultra narrow gap was defined. Secondly, based on the experimental results of up-and-down torch oscillation welding, phase shift of current and wire extension length were simulated for varying oscillation frequency to show that weld the bead shape in ultra narrow gap welding can be predicted. As the result, it was confirmed that reliable weld quality in ultra narrow gap welding can be achieved with up-and-down torch oscillation above 15Hz due to its ability to provide uniform heat input along the sidewall of gap.