• 미생물학회지
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• 제39권2호
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• pp.89-94
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• 2003

호흡기 바이러스 분리 및 항원분석을 통하여 미래의 백신개발 및 항원 분석을 위한 역학적 자료를 제공하고자 2000~2001년 사이의 급성 호흡기 감염증 환자를 대상으로 호흡기 바이러스를 분리하여 그 특성을 조사하였다. 2000년도에 분리된 바이러스는 충 43주로서, 이 중 39주의 인플루엔자바이러스가 분리되었으며, A형이 23주, B형이 16주 관찰되었으며, 또한 아데노바이러스가 4주 관찰되었다. 그리고 2001년도에는 총 56주의 바이러스가 분리되었는데, 이는 모두 인플루엔자 바이러스로 확인되었다. 분리된 인플루엔자 바이러스의 주요항원별 특징으로는 A/Sydney/05/97(H3N2)-like, A/Beijing/262/95(H1N1)-like, 및 B/Harbin07/94-like가 2000년에, A/Panama/253/99(H3N2)-like 및 A/Newcaledonia/2007/99(H1N1)-like가 2001년에 분리되었으며, 아데노바이러스는 1, 2, 5형의 혈청형이 발견되었다. 분리된 바이러스의 성별 발생분포는 2000년에 남성환자 14명(32.56%), 여성 환자 23명(67.44%), 2001년에 남성환자 23명(41.07%), 여성환자33명(58.93%)이 발생하여 2000년과 2001년 모두 여성환자의 발생율이 비교적 높게 나타났다. 연령별 발생분포는 2000년에는 0-1세 사이에서 48.84%로 대부분을 차지하였으나, 2001년에는 l1∼20세 사이에서 33.93%로 비교적 높은 발생율을 나타내었으며, 그 외 연령별로는 비슷한 발생율을 나타내었다. 월별 발생분포는 2000년에는 1월에 한차례 높았다가 다시 4월에 다소 높은 발병율을 나타내었으며, 6월까지 낮은 비율이지만 다양한 바이러스가 출현하였다. 반면 2001년에는 2월과 3월에 집중적으로 발생하였으며 H3N2가 강력한 발병율은 나타내면서 주로 이른봄에 발생하였다.

• 환경영향평가
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• 제26권3호
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• pp.181-194
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• 2017

현재 우리나라에 설치된 어도의 90% 이상이 콘크리트 인공 어도로 되어있으나, 최근 자연적인 재료를 이용하여 소하천의 형태로 설치하는 자연형 어도가 많이 설치되고 있다. 이에 따라 국내 정부산하 공기업과 대학에서는 자연형 어도에 대한 설계 기준에 등록할 목적으로 '자연형 어도 표준모형 형식(안)'을 제안하였다. 본 연구에서는 기존에 제안된 형식안을 검토해보고 보완, 개선되어야 할 사항을 제시해보았다. 연구 결과, 지금까지 국내에서 어도 설계와 관련하여 진행되었던 연구가 매우 부족해 적절한 기준을 마련하는데 한계가 있었던 것으로 판단된다. 특히 우회수로형 자연형 어도에서의 구조 유지 방안이나 내부 돌 설치 방법, 또는 자연 재료에 대한 연구가 부족한 것으로 판단되었는데, 이는 해외에서 진행되었던 연구사례에 비춰볼 때 국내에서도 국내 상황에 맞는 설계 연구가 보다 적극적으로 진행되어야 할 것으로 보인다. 또한 경사도와 폭에 대한 설계기준 역시 주로 이미 설치되어 있는 국내의 자연형 어도들을 근거로 제시되고 있는데, 이는 어종 특성은 물론 어도에 대한 수리학적, 생물학적 연구를 바탕으로 각 지역 조건에 맞는 설계기준이 새롭게 마련되어야 할 것으로 판단된다.

• Wind and Structures
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• 제26권3호
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• pp.115-128
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• 2018

Appropriate modeling of a tornado-like vortex is a prerequisite when studying the near-ground wind characteristics of a tornado and tornado-induced wind loads on structures. Both Ward- and ISU-type tornado simulators employ guide vanes to induce angular momentum to converge flow in order to generate tornado-like vortices. But in the Ward-type simulator, the guide vanes are mounted near the ground while in the ISU-type they are located at a high position to allow vertical circulation of flow that creates a rotating downdraft to generate a tornado-like vortex. In this study, numerical simulations were performed to reproduce tornado-like vortices using both Ward-type and ISU-type tornado simulators, from which the effects of rotating downdraft on the vortex characteristics were clarified. Particular attention was devoted to the wander of tornado-like vortices, and their dependences on swirl ratio and fetch length were investigated. The present study showed that the dynamic vortex structure depends significantly on the vortex-generating mechanism, although the time-averaged structure remains similar. This feature should be taken into consideration when tornado-like-vortex simulators are utilized to investigate tornado-induced wind forces on structures.

• 한국축산식품학회지
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• 제40권4호
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• pp.649-658
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• 2020

This study compared aroma compositions and sensory aroma attributes of raw and cooked Tenebrio molitor larvae (mealworms). Main sensory aroma attributes of raw mealworms were strong wet-soil-like, and less-intense oily, shrimp-like and sweet-corn-like. Quantitatively, the major aroma components of raw mealworms were hydrocarbons and aldehydes. As cooking proceeded, sweet-corn-like, roasted, and fried-oil-like sensory attributes were increasingly perceived with steaming, roasting, and frying, respectively. Some pyrazines, pyrrolidines, and carbonyls increased or appeared in roasted and fried mealworms. Partial least squares regression also showed differences in raw and cooked mealworms based on aroma components and their sensory attributes. Unlike raw mealworms, steamed mealworms had a relatively strong sweet-corn-like aroma attribute, which was related to 2,4,6-trimethyl-heptane, 2,4-dimethyl-dodecane, and 3,5-dihydroxy-6-methyl-2,3-dihydropyran-4-one. In comparison, roasted and fried mealworms exhibited roasted, shrimp-like, and fried-oil-like aroma attributes, which were associated with intermediates of the Maillard reaction and lipid oxidation, such as pyrazines, alcohols, and aldehydes. This result during thermal reactions was very similar to those of meat and/or seafood. The use of mealworms as a savory-type flavor enhancer can be expected.

• 대한의용생체공학회:의공학회지
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• 제31권6호
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• pp.481-486
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• 2010

In this paper, we suggest an advanced algorithm, to recognize pedestrian/non-pedestrian using differential haar-like feature, which applies Adaboost algorithm to make a strong classification from weak classifications. First, we extract two feature vectors: horizontal haar-like feature and vertical haar-like feature. For the next, we calculate the proposed feature vector using differential haar-like method. And then, a strong classification needs to be obtained from weak classifications for composite recognition method using the differential area of horizontal and vertical haar-like. In the proposed method, we use one feature vector and one strong classification for the first stage of recognition. Based on our experiment, the proposed algorithm shows higher recognition rate compared to the traditional method for the pedestrian and non-pedestrian.

• BMB Reports
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• 제44권6호
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• pp.387-392
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• 2011

To investigate the molecular scavenging capabilities of the larvae of Hermetia illucens, two serine proteases (SPs) were cloned and characterized. Multiple sequence alignments and phylogenetic tree analysis of the deduced amino acid sequences of Hi-SP1 and Hi-SP2 were suggested that Hi-SP1 may be a chymotrypsin- and Hi-SP2 may be a trypsin-like protease. Hi-SP1 and Hi-SP2 3-D homology models revealed that a catalytic triad, three disulfide bonds, and a substrate-binding pocket were highly conserved, as would be expected of a SP. E. coli expressed Hi-SP1 and Hi-SP2 showed chymotrypsin or trypsin activities, respectively. Hi-SP2 mRNAs were consistently expressed during larval development. In contrast, the expression of Hi-SP1 mRNA fluctuated between feeding and molting stages and disappeared at the pupal stages. These expression pattern differences suggest that Hi-SP1 may be a larval specific chymotrypsin-like protease involved with food digestion, while Hi-SP2 may be a trypsin-like protease with diverse functions at different stages.

• 정보처리학회논문지A
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• 제19A권4호
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• pp.175-180
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• 2012

본 논문은 Haar-like 특징을 이용한 고성능 보행자 및 차량 인식 회로를 제안한다. 제안하는 회로는 영상의 매 프레임 마다 슬라이딩 윈도우를 적용하여 Haar-like 특징을 추출하고 보행자 및 차량을 인식한다. Haar-like 특징 추출 회로는 슬라이딩 윈도우 당 200개의 Haar-like 특징을 추출하며, 추출된 특징들은 AdaBoost 인식 회로에서 사용된다. 제안하는 회로는 속도 향상을 위해 병렬 회로 구조를 적용하였으며 두 개의 슬라이딩 윈도우가 동시에 보행자 또는 차량을 인식한다. 제안하는 고성능 보행자 및 차량 인식 회로는 Verilog HDL로 설계하였으며 130nm 표준 셀 라이브러리를 이용하여 게이트 수준의 회로로 합성하였다. 합성된 회로는 1,388,260개의 게이트로 구성되며 최대 동작 주파수는 203MHz이다. 제안하는 회로는 $640{\times}480$ 영상을 초당 약 47.8장 처리할 수 있기 때문에 보행자와 차량을 실시간으로 인식하기 위해 사용될 수 있다.

• Journal of Life Science
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• 제11권2호
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• pp.103-107
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• 2001

The equine chorionic gonadotropin (eCG) subunits $\alpha$ and ${\beta}$ are transcribed from different genes and associate noncovalently to form the bioactive eCG heterodimer. Dimerization is rate limiting for eCG secretion, and dissociation leads to hormone inactivation. The correct conformation of the heterodimer is alto important for efficient secretion, hormone-specific post-translational modifications, receptor binding and signal transduction. To determine whether ${\alpha}$ and ${\beta}$ subunits can be synthesized as a single polypeptide chain (tethered-eCG) and also display biological activity, the tethered-eCG molecule by fusing the carboxyl terminus of the eCG ${\beta}$-subunit to the amino terminus of the af-subunit was construe-ted and transfected into chinese hamster ovary (CHO-Kl) cells. LH- and FSH-like activities were assayed in terms of testosterone production and aromatase activity in primary cultured rat Leydig cells and granulosa cells, respectively. The tethered-eCG was efficiently secreted and showed similar LH-like activity to the dimeric eCG ${\alpha}$/${\beta}$ and native eCG. FSH-like activity of the tethered-eCG was also shown similarly in comparison with the native and wild type eCG ${\alpha}$/${\beta}$. Our data for the first time suggest that the tethered-eCG can be expressed efficiently and the produced product by the CHO-K1 cells is fully LH- and FSH-like activities in rat in vitro bioassay system. Our results also suggest that this molecular can imply particular models of FSH-like activity not LH-like activity in the eCG. Taken together, these data indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion.

• 한국인쇄학회지
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• 제30권3호
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• pp.65-75
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• 2012

Appearing gagman on various ads are presently experienced in domestic market both high involvement and low involvement products ads. Therefore, this research attempts to analyze the effectiveness of using gagman ad model in terms of building brand preference and purchasing intention among target audience. Results of this study proposed the basement of practical use when gagman is selected as a ad model in the field. For testing hypotheses attractiveness, reliability, like/dislike as dependent variables and as independent variable purchasing intention were selected. The result of analysis shows that for high involvement product reliability influenced on brand like/dislike and attractiveness of gagman model is effective to enhance purchasing intention. For low involvement product only like/dislike was significant. This can be interpreted gagman ad model is useful for building brand like/dislike and purchasing intention of high involvement product when gagman has reliability and attractiveness respectively, while for low involvement product, like and dislike is the only variable to be considered in choosing gagman ad model.

• Bulletin of the Korean Chemical Society
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• 제31권8호
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• pp.2265-2271
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• 2010

In this study, wormorm-like polymeric micelles were construted from poly(L-lactic acid)-b-poly(ethyelen glycol) (PLLA-b-PEG) block copolymers via worm-like (or cylindrical) self- assembly that consisted of a relatively long PLLA block ($M_n$ 7K Daltons) at the core and a relatively short PEG block ($M_n$ 2K Daltons) as the shell. Several cancer-targeting moieties (such as folate, cobalamin, and cyclic arginine-glycine-aspartic (RGD) peptide) were chemically coupled with the succinylated or maleimided PEG block of PLLA-b-PEG to act as a cancer cell-specific targeting ligand for breast cancer. The worm-like micelles with muplite cancer cell-specific ligands proved to be successful in recognizing different breast cancer cells at once. This has the potential to aid in cancer-specific drug delivery and to be used as an effective treatment for breast cancer.