• Korean journal of applied entomology
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• v.53 no.1
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• pp.27-34
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• 2014

Entomopathogenic fungi were isolated from Bemisia tabaci in an Oriental melon field, and their growth characteristics, factors related to a natural outbreak, and infectivity against Bemisia tabaci, Tetranychus urticae, and Frankliniella intonsa were investigated. The isolates had erect conidiophores bearing whorls of 4-6 phialides with a swollen base where cylindrical conidia of $3.0-3.4{\mu}m$ were attached. The isolates were identified as Isaria fumosorosea on the basis of morphological characteristics and an ITS sequence with 99% similarity. I. fumosorosea IFs-08 grew well on Sabouraud dextrose agar+yeast extract medium(3.2 mm/day/$24^{\circ}C$); it grew better at $35^{\circ}C$ than at $15^{\circ}C$. The isolates of I. fumosorosea-IFs were highly infective and killed 93.9-96.7% B. tabaci, 84.9-92.0% T. urticae, and 81.5-84.4% F. intonsa in bioassay, whereas three isolates (Isaria tenuipes, Isaria farinosa, and Isaria fumosorosea) from KACC showed a low infectivity of 10-20%. To the best of our knowledge, this is the first report of I. fumosorosea isolated from B. tabaci in Korea.

• Food Science and Preservation
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• v.30 no.5
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• pp.875-884
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• 2023

In this study, the quality characteristics of texturized vegetable proteins (TVP) produced from defatted soy flour (DSF) were analytically compared with those of texturized vegetable proteins produced with isolated soy protein (ISP) and non-defatted soy flour (SF). The base raw material formulation consisted of 50% soy proteins, 30% gluten, and 20% corn starch. A cooling die-equipped extruder was used with a barrel temperature set at 190℃ and screw rotation speed of 250 rpm. With respect to the hardness of isolate soy proteins, that of soy flour and defatted soy flour was 22.4% and 68.8%, respectively, and gumminess was 17.6% and 44.3%, respectively. Defatting increased chewiness, shear strength, and springiness. Moisture content was higher in soy flour than in defatted soy flour, while there were no significant differences in terms of water absorption and turbidity. The pH was higher with soy flour than with defatted soy flour. Concerning color, the L and b values were higher with soy flour, while the a value was higher with defatted soy flour. These results suggest that defatting soybeans can improve the quality of plant-based proteins. Further research is needed to address the quality differences from those of isolated soy proteins.

• Earthquakes and Structures
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• v.3 no.2
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• pp.169-180
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• 2012

The performance of pure friction isolation system with respect to the frequency bandwidth of excitation and the predominant frequency is investigated. A set of earthquake ground motions (artificial as well as recorded [with different combinations of magnitude-distance and local site geology]) is considered for investigating effectiveness of pure friction isolators. The results indicate the performance of pure friction base isolated system does not only depend upon coefficient of friction and mass ratio but the stick-slip behaviour depends upon the frequency content of the excitation as well. Slippage prevails if the excitation frequency lies in a suitable frequency range. This range widens with increasing mass ratio. For larger mass ratios, the sliding effect is more pronounced and the maximum acceleration response is further reduced in the neighbourhood of frequency ratio (${\omega}/{\omega}_n$) of unity. The pure friction isolation system is effective in the case of broadband excitations only and that too, in the acceleration sensitive range of periods. The pure friction system is not effective for protection against narrow band motions for which the system response is quasi-periodic.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 2004.04a
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• pp.33-40
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• 2004

본 논문에서는 한국과 일본에 위치한 여러 연구기관들 사이에서 수행된 온라인 네트워크 유사동적 실험결과에 대해 나타내었다. 예제 구조물로는 4경간 연속의 면진 교량을 이용하였다. 실험 장비를 보유하고있는 두 연구기관에서 면진 장치의 비선형 거동에 대한 실험을 수행하고 각 실험 결과를 조합하여 전체구조의 동적 해석을 수행하였다. 본 논문에서는 먼저 인터넷을 이용한 두 가지 데이터 전송기법을 이용하여 두 기법의 효율성을 비교, 분석하였다. 또한 최근 국내에 위치한 두 연구기관 사이에서 수행된 실험 결과에 대해 논의하였다. 마지막으로 유선과 무선 인터넷을 이용한 온라인 실험 기법에 대하여 나타내었다. 그 결과, 온라인 네트워크 실험에 소요된 실험 시간은 데이터 전송 기법과 실험장비에 따라 매 시간 단계의 데이터 전송에 0.2-15초, 각 연구기관의 유사동적 실험에 1-10초의 시간이 소요되어 매우 큰 폭으로 변화함을 알 수 있었다. 또한 무선 인터넷을 이용한 온라인 실험의 경우, 뛰어난 이동성과 인터넷 보안성 등과 같은 여러 가지 장점을 가지고 있음을 알 수 있었다.

• Journal of the Society of Naval Architects of Korea
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• v.55 no.4
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• pp.321-329
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• 2018

Resonance phenomena occurs at large vertical pump which is operating to cool down the hot steam using sea water in the power plant. To avoid the resonance, the natural frequency needs to be isolated about 20% from motor operating speed. Yet, excessive vibration occurs especially at low tide. At first, natural frequency of the whole pump system and each part is calculated using ANSYS. As it is revealed in the previous journal papers that only circular pipe part is related to resonance, the FSI technique is applied for free vibration analysis. The natural frequency is reduced to 60% (compared to that) of the frequency measured in air as it is similar to other published results. And the frequency obtained by finite element analysis is almost same to that obtained from modal test. Based on the accurate finite element model and analysis, design change is tried to avoid the resonance by changing the thickness of pipe and base supporting plate. In stead of doing optimization process, design sensitivity is computed and used to find such designs to avoid resonance.

• Journal of Life Science
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• v.8 no.3
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• pp.348-351
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• 1998

Common DNA fragments of the ${\beta}$-globin gene were observed from six races of the adult common carp: Hybrid-Yamato, Japanese wild type, Mirror, Suwa-Yamato, Scale German, and Saku-Yamato. Chromosomal DNAs isolated from the above six races were digested with restriction endonucleased EcoRI and PstI. The digested fragments were transferred onto nitrocellulose filter and hybridized with a probe of carp ${\beta}$-globin cDNA. Molecular sizes of the hybridized DNA fragments digested with EcoRI were 3.6Kb(Kilo base), 4.3Kb and 15Kb in Hybrid-Yamato, Japanese wild type, Mirror, Scale German and Saku-Yamato carp DNAs. In Scale German and Saku-Yamato carp DNAs, two and one more hybridized DNA fragments were observed, respectively. Molecular sizes of the hybridized DNA fragments digested with PstI were 2.2Kb, 6.5Kb, 7.8Kb and 9.2Kb in Hybrid-Yamato, 2.2Kb, 6.5Kb and 9.2Kb in Japanese wild type, 2.2Kb, 6.5Kb, 7.8Kb, and 13Kb in Mirror, 2,2Kb, 5,5Kb, 6.5Kb, 7.8Kb, 9.2Kb and13Kb in Scale German, and 2.2Kb, 5.5Kb, 6.5Kb, 9.2Kb and Saku-Yamato carp DNA. Therefore, depending on carps, three to six DNA fragments were hybridized with ${\beta}$-globin gene probe. Thus it indicated polymorphysm in the globin gene family of carp.

• Korean Journal Plant Pathology
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• v.11 no.3
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• pp.282-284
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• 1995

Grey leaf spot on tomato plants was first observed in Sedo-myeon, Puyo-kun, Chungnam province of Korea in 1994. This disease which had not been reported before in Korea exhibited different symptoms from those of other leaf spot diseases on tomato plants. The symptoms were characterized by small irregular-shaped spots on leaves at the initial stage of the infection, subsequent spread and coalescence of the spots throughout the leaves with ultimate necrosis, and abscission from the plants. When healthy tomato plants were inoculated with a conidial suspension of the fungus isolated from the lesion of a diseased plant in a field, the same characteristic symptoms as those in the field were produced. Furthermore, the same pathogen could be reisolated from the lesions formed buy the inoculation. Conidial characteristics of the pathogen were as follows; oblong shape with constricted 3 transverse septa, round-shaped base, round- or point-shaped apex, size of 45~75$\times$12.5~17.5 ${\mu}{\textrm}{m}$, and 3.5 : 1 ratio of length to width. The pathogen was identified as Stemphylium lycopersici and thus this is the first report on the occurrence of grey leaf spot disease on tomato plants caused by s. lycopersici in Korea.

• Korean Journal of Microbiology
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• v.27 no.3
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• pp.194-200
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• 1989

The nucleotide sequence of inducible chloramphenicol acetyl-transferase(CAT) gene isolated from a small plasmid pSBK203 of Staphylococcus aureus was determined. The base sequence shows that structural gene of pSBK203-CAT encodes a protein of 213 amino acids and has a leader region which encodes a short polypeptide of 9 amino-acids in its upstream. vertical bar /sup 35/S vertical bar-Methionine labelled CAT gene product in minicell showed almost same mobility with pC194-CAT of which molecular weight is 24Kdal on polyacrylamide gel electrophoresis. Predicted amino acid sequence of pSBK203-CAT has revealed a high degree of homology with the CATs of pC194 and pC221 than those of cat-86, Tn9 and proteus mirabilis PM13.

• Korean Journal of Microbiology
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• v.28 no.1
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• pp.27-34
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• 1990

Two macrolide-lincosamide-streptogramin B (MLS) antibiotic resistance genes, one expressed inducibly and the other expressed constitutively were recognized from a single Staphylococcus aureus DH1 strain. The inducible MLS resistance gene was isolated and cloned from the R-plasmid pDE1(7.4kb) and the constitutive gene was from chromosomal DNA. Base sequence of the inducible MLS resistance gene (1.2kb) was determined and found as same that of pE194. The restriction map of the cloned constitutive MLS resistance gene was compared with that of the inducible gene. Two genes have same restriction map except leader region. In the constitutive gene there is no leader region which is doing major role in inducible expression.

• Korean Journal of Microbiology
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• v.31 no.2
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• pp.99-104
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• 1993

Two strains of the gram-negative acetic acid bacteria, Acetobacter sp. strain CS2- AND CS5, were isolated form the traditional raw rice wine vinegar of Haenam area. The strains oxidized ethanol to acetic acid and over-oxidized acetate and lactate to $CO^{2}$ and $H ^{2}$O. They produced 2-ketogluconic acid from glucose but did not produce .gamma.-pyrones from glucose and dihydroxyacetone from glycerol. The CS strains possessed ubiquinone-9 as a major isoprenoid quinone and contained straight-chain $C_{18 :1}$, $_C{16 : 0}$, and $C _{14 : 0}$ fatty acids. The DNA base composition of the CS2 and CS5 strains was 56.2 and 57.3 mole% G + C, respectively. The isolates were grown well on methanol, gluconate, erythritol, raffinose, dulcitol and xylitol as sole sources of carbon and energy which are different from those of other Acetobacter species and producedd acid from sucrose, glycerol, fructose, inositol, mannitol, and ribose.