• Proceedings of the Korean Nuclear Society Conference
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• 2005.05a
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• pp.747-748
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• 2005

• Proceedings of the Korean Nuclear Society Conference
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• 1999.10a
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• pp.373.2-373
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• 1999

• Korean Journal of Microbiology
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• v.32 no.1
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• pp.20-27
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• 1994

An inducible ${\beta}$-lactamase gene (bla) was identified and isolated from the chromosomal DNA of multiple drug resistant strains of Staphylococcus aureus. Determined base sequence of bla and of its flanking region was compared with those of bla genes identified on the staphylococcal plasmids pPC1, pI258, pI1071, and pUB101. Base sequence of 843 base-long structural gene of our bla was same as that of pPCl-, pI258-, and pS1-bla. However, HindIII recognition site Which is found in most of the bla genes at 140 base upstream from the structural gene was moved to the site of 370 base upstream from the structural gene. And one of the two direct repeat sequence found in downstream flanking region of pI1071-bla was deleted in our bla. Amino acid sequence homology analysis of the ORF located around HindIII recognition site reveals that this 80 amino acids-long polypeptide is C-terminus of transposase of Tn4001.

• Earthquakes and Structures
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• v.5 no.5
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• pp.499-526
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• 2013

Seismic isolation is an effective method for the protection of buildings and their contents during strong earthquakes. This research work aims to assess the appropriateness of the linear and nonlinear models that can be used in the analysis of typical low-rise base isolated steel buildings, taking into account the inherent nonlinearities of the isolation system as well as the potential nonlinearities of the superstructure in case of strong ground motions. The accuracy of the linearization of the isolator properties according to Eurocode 8 is evaluated comparatively with the corresponding response that can be obtained through the nonlinear hysteretic Bouc-Wen constitutive model. The suitability of the linearized model in the determination of the size of the required seismic gap is assessed, under various earthquake intensities, considering relevant methods that are provided by building codes. Furthermore, the validity of the common assumption of elastic behavior for the superstructure is explored and the alteration of the structural response due to the inelastic deformations of the superstructure as a consequence of potential collision to the restraining moat wall is studied. The usage of a nonlinear model for the isolation system is found to be necessary in order to achieve a sufficiently accurate assessment of the structural response and a reliable estimation of the required width of the provided seismic gap. Moreover, the simulations reveal that the superstructure's inelasticity should be taken into account, especially if the response of the structure under high magnitude earthquakes is investigated. The consideration of the inelasticity of the superstructure is also recommended in studies of structural collision of seismically isolated structures to the surrounding moat wall, since it affects the response.

• Structural Engineering and Mechanics
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• v.75 no.1
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• pp.33-47
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• 2020

In this paper the dynamic behavior of an isolated building subjected to idealized near-fault pulses is investigated. The building is represented with a simple 2-DOF model. Both linear and non-linear behavior of the isolation system is considered. Using dimensional analysis, in conjunction with closed form mathematical idealized pulses, appropriate dimensionless parameters are defined and self-similar curves are plotted on dimensionless graphs, based on which various conclusions are reached. In the linear case, the role of viscous damping is examined in detail and the existence of an optimum value of damping along with its significant variation with the number of half-cycles is shown. In the nonlinear case, where the behavior of the building depends on the amplitude of the excitation, the benefits of dimensional analysis are evident since the influence of the dimensionless 𝚷-terms is easily examined. Special consideration is given to the normalized strength of the non-linear isolation system that appears to play a complex role which greatly affects the response of the 2-DOF. In the last part of the paper, a comparison of the responses to idealized pulses between a linear fixed-base SDOF and the respective isolated 2-DOF with both linear and non-linear damping is conducted and it is shown that, under certain values of the superstructure and isolation system characteristics, the use of an isolation system can amplify both the normalized acceleration and displacement of the superstructure.

• Restorative Dentistry and Endodontics
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• v.20 no.2
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• pp.577-609
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• 1995

Bacteria have been regarded as one of the most important factors in pulpal and periapical diseases. Streptococci are frequently isolated facultative anaerobes in infected root canals. Recently molecular biological techniques have been rapidly progressed. This study was designed to apply the molecular biological tools to the identification and classification of streptococci in the endodontic microbiology. Streptococci isolated from infected root canals were identified with both Vitek Systems and API 20 STREP. Identification results were somewhat different in several strains of streptococci. Eighteen streptococci and enterococcal was difficult so to digest plasmid DNA using Hind III and EcoRI to differentiate strains by restriction enzyme analysis of plasmid DNA. 16S rDNA of chromosome was amplified by polymerase chain reaction(PCR) and then restricition fragment length polymorphism(RFLP) using several restriction enzymes was observed. The molecular mass of 16S rDNA of chromosomal DNA was approximately 1.4kb. There were three to five RFLP patterns using eight restriction enzymes. RFLP patterns digested with CfoI which recognizes four base sequences were identical in all stains. Hind III which recognizes six base sequences could not digest the 16S rDNA. Restriction enzymes which recognize five base sequences were suitable for RFLP pattern analysis. At least three different restriction enzymes were needed to compare each strains. 16S rDNA PCR-RFLP was simple and rapid to differentiate and classify strains and could be used in the epidemiological study of root canal infections.

• Earthquakes and Structures
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• v.13 no.2
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• pp.119-130
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• 2017

This paper presents a numerical investigation on the seismic behavior of isolated bridges with supplemental viscous damping. Usually very large displacements make seismic isolation an unfeasible solution due to boundary conditions, especially in case of existing bridges or high risk seismic regions. First, a suggested optimal design procedure is introduced, then seismic performance of three real bridges with different isolation systems and damping levels is investigated. Each bridge is studied in four different configurations: simply supported (SSB), isolated with 10% damping (IB), isolated with 30% damping (LRB) and isolated with optimal supplemental damping ratio (IDB). Two of the case studies are investigated under spectrum compatible far-field ground motions, while the third one is subjected to near-fault strong motions. With respect to different design strategies proposed by other authors, results of the analysis demonstrated that an isolated bridge equipped with HDLRBs and a total equivalent damping ratio of 70% represents a very effective design solution. Thanks to confirmed effective performance in terms of base shear mitigation and displacement reduction under both far field and near fault ground motions, as well as for both simply supported and continuous bridges, the suggested control system provides robustness and reliability in terms of seismic performance also resulting cost effective.

• Structural Engineering and Mechanics
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• v.34 no.5
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• pp.663-666
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• 2010

The spectral content of ground displacement of the 10 largest last California earthquakes is studied. Specifically, the power spectral density function of the critical horizontal-component record of the closest-to-epicenter station is analyzed in each case. The results permit to state that horizontal ground displacement is a narrow-band process. This fact was previously noticed while trying to solve the large-base-displacement problem in isolated structures and it was fundamental in the solution of this issue; however, these preliminary results were limited in number to enable a statement like the foregoing one. Thus, the broader results presented herein were necessary.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.502-505
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• 2004

Now for the first time in Korea pilot project on application of base isolation system to the RC building is carrying out by collaboration with KNHC and DRB dongil. The hybrid-type base isolation system, which is composed of sliding bearings and laminated rubber bearings and can make the resonance period of base isolated buildings comparatively long up to 4 or 5 seconds, is applied to this building. In this paper the overview of this project, the dynamic characteristics of this particular building and the response reduction effect against earthquakes are presented.

• Korean Journal of Microbiology
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• v.31 no.1
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• pp.44-47
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• 1993

A replication initiation gene was identified and its nucleotide sequence has been determined from a 3.8 kb, chloramphenicol acethyltransferase conferring R-plasmid pSBK203 of Staphylococcus aures. Location of the replication related region of pSBK 203 was determined by interuption with pUC 119 at XBaI and MspI sites which resulted in inactivation of replication in Bacilius subtilis. Base sequence of this region revealed on open reading frame of 942 base pairs, which encoded a 314 amino acid protein. Base sequence homology with other rep of pT181 family plasmids such as pT181, pC221, pC223, pS194, pU112, and pCW7 was ranged from 78% to 97% and the predicted amino acid sequence homology was from 72% to 95%.