• Journal of Microbiology and Biotechnology
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• 제32권7호
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• pp.825-834
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• 2022

Inflammatory bowel disease (IBD) is a global disease that is in increasing incidence. The gut, which contains the largest amount of lymphoid tissue in the human body, as well as a wide range of nervous system components, is integral in ensuring intestinal homeostasis and function. By interacting with gut microbiota, immune cells, and the enteric nervous system, the intestinal barrier, which is a solid barrier, protects the intestinal tract from the external environment, thereby maintaining homeostasis throughout the body. Destruction of the intestinal barrier is referred to as developing a "leaky gut," which causes a series of changes relating to the occurrence of IBD. Changes in the interactions between the intestinal barrier and gut microbiota are particularly crucial in the development of IBD. Exploring the leaky gut and its interaction with the gut microbiota, immune cells, and the neuroimmune system may help further explain the pathogenesis of IBD and provide potential therapeutic methods for future use.

• 대한한의학회지
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• 제30권1호
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• pp.83-94
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• 2009

Background and Objectives: The skin barrier protects skin against a harmful environment. Its function includes an antimicrobial barrier as well as a physical barrier. Stress is harmful to the skin barrier and there are many studies on this, but there are few about the effect of stress during pregnancy on the skin barrier of offspring. The aim of this study was to investigate the effect of stress during pregnancy on the skin barrier of offspring by examination with the naked eye, cortisol, weight, TEWL and histologic tests. Materials and Methods: Male hairless mice ten weeks old were coupled with females for three days. After twelve days the females were divided into two groups. We stressed one group and not the other group. The offspring from the non-stressed (N group) and stressed (S group) grew up until four weeks. The S group was exposed to chronic mild stress using the chronic stress model protocol modified method of Wilner and Towell for a week. We made examinations with the naked eye, assessment of cortisol, weight, TEWL, and histologic test to contrast the S group with the N group. Statistical analysis was performed by using Mann-Whitney and Friedman test. Statistical significance was achieved if the probability was less than 5% (p<0.05). Results: 1. By making an examination with the naked eye, S group showed papules, creases and dryness on their skin, but N showed no change compared with normal skin. 2. There was no statistically significant difference between S group and N group in cortisol (p>0.05). 3. There was no statistically significant difference between S group and N group in weight. N group had less weight than S group (p<0.05). 4. There was no statistically significant difference between S group and N group in TEWL (p>0.05). 5. There was no statistically significant difference between S group and N group in TEWL recovery (p>0.05). 6. In TEWL and TEWL recovery, there was statistically significant difference by time between S group and N group (p<0.005). Histologically, S group showed a thicker epidermis and epidermal crest than N group. Conclusions: We found that stress during pregnancy has negative effects on the skin barrier of offspring. Though there were no statistically significant differences between S group and N group in TEWL recovery, we found evidence that the recovery of skin barrier function is interrupted by stress during pregnancy by examination with both the naked eye and histology.

• 대한임상검사과학회지
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• 제49권4호
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• pp.395-400
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• 2017

아토피 피부염은 만성염증 피부질환으로서 유전적 요소, 환경적 요소, 면역반응의이상, 피부장벽단백질의 기능 이상에 의하여 발생한다. 본 연구의 목적은 피부장벽단백질의 발현을 측정할 수 있는 ELISA 키트 개발에 있다. AriNo와 D-Squame 패치를 이용하여 비침습적으로 피부에서 단백질을 얻을 수 가 있었고, AriNO가 좀 더 많은 단백질을 획득하였다. 0.1% Triton X-100용액이 다른 용해용액인 0.1 M Tris-HCL, 5 mM KOH, 0.1% Tween-20보다 높은 단백질 수율을 나타냈다. 피부장벽 단백질 측정을 위한 ELISA 키트 개발을 위하여 분자생물학적인 방법을 이용하여 필라그린과 인보루크린의 재조합단백질을 생산하였고, 이에 대한 단일클론항체를 면역학적인 방법을 이용하여 만들었다. 아토피 피부염 환자의 피부에서는 필리그린의 발현이 유의하게 줄어들었고, 인보루크린은 정상인과 아토피 피부염 환자에서 차이를 나타내지 않았다. 본 연구의 결과를 통하여 아토피 피부염에서 피부장벽단백질의 중요성을 규명하였고, 향후 아토피 피부염의 진단키트를 개발하는데, 유용할 것이다.

• Perspectives in Nursing Science
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• 제10권1호
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• pp.12-23
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• 2013

Purpose: This study was conducted to investigate ICU nurses' perceptions of communication difficulties, the importance of and satisfaction with communication with doctors, other nurses, patients, and family, as well as to explore communication barrier with patient families. Methods: Investigators developed a 15-item communication perception questionnaire and 58-item communication barrier questionnaire. Communication barrier included 4 domains: nurses, family, environment, and patient condition. A total of 151 ICU nurses with a minimum of one year of ICU experience participated. Results: ICU patients ($3.38{\pm}0.73$) were the most difficult group to communicate with, followed by family ($3.32{\pm}0.72$), senior nurses ($3.25{\pm}0.74$), doctors ($3.21{\pm}0.68$), and nurse colleagues ($2.64{\pm}0.73$). Doctors ($4.61{\pm}0.53$) were the most important group to communicate with, followed by nurse colleagues ($4.52{\pm}0.54$), patients ($4.49{\pm}0.58$), senior nurses ($4.44{\pm}0.55$), and family ($4.43{\pm}0.61$). Satisfaction with communication was the highest with colleague nurses ($3.60{\pm}0.68$), then senior nurses ($3.37{\pm}0.74$), family ($3.18{\pm}0.71$), patients ($3.09{\pm}0.75$), and doctors ($3.06{\pm}0.83$).The total score of the communication barrier was $2.83{\pm}0.52$, where each domain was scored as follows: patient condition $3.13{\pm}0.74$, nurses $2.83{\pm}0.60$, environment $2.81{\pm}0.66$, and family $2.76{\pm}0.57$. The ICU nurses reported that communication was difficult due to 'sudden deterioration in the patient's condition', 'being too busy', 'a noisy environment', and 'information not being shared between family members.' Significant differences were noted by age, clinical experience, and marital status of nurse respondents. Conclusion: The findings indicated that development of a protocol on communication between nurses and doctors as well as development of an educational program on communication skills are necessary.

• 한국수자원학회논문집
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• 제47권11호
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• pp.1087-1094
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• 2014

해안지역에서 지하수 해수침투 저감을 위하여 해수 양수 관정을 가동할 때 해수정으로 많은 양의 담수가 낭비되는 사례가 있다. 해수 침투를 저감시키고 낭비되는 담수를 유용하게 활용하기 위하여 이중 양수정이 제안된 바 있다. 이중 양수정은 해수 양수를 위한 해안 쪽의 해수정과 담수 양수를 위한 내륙 쪽의 담수정으로 구성된다. 선행 연구에서는 담수정의 해수 비율을 최소화시키기 위한 해수정의 양수량을 산정하였으나 담수정의 해수비율이 1~15% 범위로 나타나 담수정에서 담수를 확보하기 어렵다고 판단하였다. 본 연구에서는 이송이 지배적인 상황에서 이중 양수정의 효과를 분석하였다. 연구 결과 담수정에서 저염도 지하수를 확보할 수 있음을 밝혔다. 또한 해안 유역 차원의 적정 지하수관리를 위해서는 담수정의 해수비율을 최소화하는 것 외에도 해수정으로 낭비되는 담수량을 최소화하는 것도 함께 고려해야한다.

• 한국표면공학회지
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• 제47권6호
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• pp.316-322
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• 2014

With increase in demand for higher operating temperatures of gas turbines, extensive research efforts have been carried out to enhance the performance of thermal barrier coatings (TBCs) in the field of coating processing as well as materials. In this study, thermal barrier coatings in lanthanum/gadolinium zirconate system, which is one of the most promising candidates for replacing yttira-stabilized zirconia (YSZ) in thermal barrier coating applications, are fabricated via suspension plasma spray. Dense, $300{\sim}400{\mu}m$ thick coatings of fluoritephase zirconate with modest amount of segmented microstructures are obtained by using suspension plasma spray with suspensions of planetary-milled mixture between lanthanum and/or gadolinium oxide and nano zirconia. These coatings exhibit thermal conductivities of 1.6 ~ 1.7 W/mK at $1000^{\circ}C$, which is relatively lower than that of YSZ.

• 한국지반공학회논문집
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• 제35권8호
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• pp.5-15
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• 2019

이 연구는 오염원 유출을 방지하기 위한 해상폐기물매립장 차수시스템에 요구되는 적절한 기준을 제안하기 위해 수행되었다. 차수시스템은 바닥층과 연직벽으로 구성된다. 바닥층은 불투수 점토층으로 연직벽은 이중벽체로 외곽벽체는 강관쉬트파일로 외력에 대한 저항을 담당하고 연직내부벽체가 차수를 담당하는 것으로 가정하였다. SEEP/W와 CTRAN/W 프로그램을 이용하여 침투-이류-분산 해석을 정상류 및 비정상류 조건에서 실시하였다. 해석결과, 정상류 조건에서 비정상류 조건 보다 오염원 이동이 크게 나타나 설계관점에서 차수기준은 정상류조건에서 산정된 값을 적용하는 것이 타당한 것으로 나타났다. 바닥층의 점토지반이 균질하고 연직차수시스템의 시공에 문제가 없다는 전제하에 정상류조건에서 오염원 이동의 차수에 요구되는 점토층 및 연직차수벽의 투수계수, 두께, 근입깊이 등이 제안되었다.

• Journal of Magnetics
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• 제9권1호
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• pp.17-22
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• 2004

We studied the magnetotransport properties of tunnel junctions with AlO and AlN barriers fabricated using microwave-excited plasma. The plasma nitridation process provided wider controllability than the plasma oxidization for the formation of MTJs with ultra-thin insulating layer, because of the slow nitriding rate of metal Al layers, comparing with the oxidizing rate of them. High tunnel magnetoresistance (TMR) ratios of 49 and 44% with respective resistance-area product $(R{\times}A) of 3 {\times} 10^4 and 6 {\times} 10^3 {\Omega}{\mu}m^2$ were obtained in the Co-Fe/Al-N/Co-Fe MTJs. We conclude that AlN is a hopeful barrier material to realize MTJs with high TMR ratio and low $R{\times}A$ for high performance MRAM cells. In addition, in order to clarify the annealing temperature dependence of TMR, the local transport properties were measured for Ta $50{\AA} /Cu 200 {\AA}/Ta 50 {\AA}/Ni_{76}Fe_{24} 20 {\AA}/Cu 50 {\AA}/Mn_{75}Ir_{25} 100 {\AA}/Co_{71}Fe_{29} 40 {\AA}/Al-O$ junction with $d_{Al}= 8 {\AA} and P_{O2}{\times}t_{0X}/ = 8.4 {\times} 10^4$ at various temperatures. The current histogram statistically calculated from the electrical current image was well in accord with the fitting result considering the Gaussian distribution and Fowler-Nordheim equation. After annealing at $340^{\circ}C$, where the TMR ratio of the corresponding MTJ had the maximum value of 44%, the average barrier height increased to 1.12 eV and its standard deviation decreased to 0.1 eV. The increase of TMR ratio after annealing could be well explained by the enhancement of the average barrier height and the reduction of its fluctuation.

• 상하수도학회지
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• 제18권4호
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• pp.529-536
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• 2004

Innovated technique to inactivate microorganisms has been developed. This technique uses plasma discharge in 2-phase (Air-Water). Dielectric Barrier (two phase) Discharging system is able to produce new oxidants for microorganisms. Products from discharging are $HNO_2$, $NO_2{^-}$, $HNO_3$, $NO_3{^-}$ and ozone but many other radicals can be generated as well. DBD water has low concentration of ozone (about 0.5mg/L), $NO_2{^-}$, $NO_3{^-}$ (about 10mg-N/L, 20mg-N/L respectively) and lots of $H^+$. These products play an important role in oxidation. Oxidation power by KI titration methods is approximately equivalent to $50mg-O_3/L$. Surprisingly stored DBD water could oxidize KI and maintain stable pH (about pH3) even after several days. Stored DBD water for 5 days has also more than 4log disinfection power to E. coli. However, DBD water cannot be used for drinking water directly due to it's toxicity. Additional process to neutralize pH and decrease toxicity must be applied.

• 한국간호교육학회지
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• 제22권3호
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• pp.334-344
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• 2016

Purpose: The study was conducted to investigate the effect of career barrier, satisfaction, and career decision making pattern on career search behaviors in male nursing students. Methods: Data were collected through structured questionnaires that included general characteristics, career barrier, satisfaction, career decision making pattern and career search behaviors and were analyzed by descriptive statistics, ANOVA, t-test, Pearson correlation coefficient and multiple regression using SPSS/PC+ program. Results: The results of the research suggest the two aspects of career search behaviors as being self-exploration and environmental exploration. The rational pattern, among the types of satisfaction and career decision making pattern, was found to have a meaningful effect on self-expression. On the other hand, dependent pattern, among the satisfaction and career decision making pattern, was found to have an effect on career search patterns. Conclusion: It is considered that, based on the results of this study, diversified approaches that consider individual career decision making patterns such as rational pattern and dependent pattern as well as the methods to enhance the satisfaction of male nursing college students for career guidance are needed.