• BMB Reports
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• 제33권5호
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• pp.385-390
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• 2000

Overwintering plants should survive the various biotic and abiotic stresses that occur during winter. Previous studies indicated that active oxygen species are involved in freezing, dehydration, anoxia and pathogen infections. As the importance of the events that occur in the intercellular compartment became apparent in disease resistance, we examined the nature of intercellular antioxidant enzymes in order to access their possible involvement in the winter hardiness of barley. The levels of intercellular peroxidase, catalase, and SOD activities on the unit protein basis were 394, 18, and 9% of those of cellular activities, respectively. Major intercellular peroxidase isoforms consisted of four neutrals and four basic forms; whereas major cellular isoforms were two basic forms. Out of the two major catalase isoforms a higher molecular weight form was predominantly abundant in both cellular and intercellular compartments. Among the five major cellular SOD isoforms, three were also present in the intercellular compartment. The presence of substantial amounts of intercellular antioxidant enzymes in overwintering barley leaves may suggest the involvement of these enzymes in the tolerance mechanism to the various stresses that occur during winter.

• 한국초지조사료학회지
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• 제4권1호
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• pp.23-27
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• 1983

가을 김장철에 일시적으로 생산되는 채소잎으로 품질이 좋은 양질 Silage를 생산하기 위하여 본 시험을 실시 하였으며 시험구를 6개구로 설치하여 시험한 결과를 요약하면 1. 채소잎70%, 볏짚20,% 맥강10% 혼합구가 Silage질이 가장 좋았고 유기산 생성량도 많고 낙산 함량은 없었으며 타구에 비하여 가장 우수하였다. 2. 맥강을 첨가한구는 첨가치 않은 구에 비하여 기호성이 좋았고 조단백질 함량도 높았다. 3. 유기산 조성에의한 Silage품질은 총 산함량에 비하여 낙산 생성이 최하 $0{\sim}120%$ 정도로 나타냈고 반면 양질의 Silage 조제에 필요한 유기산 함량은 $53{\sim}90%$로 양호하였고 PH는 기대 한바 보다 높았다. 4. 외관상으로 볼 때 색깔, 냄새, 촉감 등이 양호한 편이고 기호성도 좋았다. 결론적으로 채소잎은 훌륭한 Silage의 재료이다.

• Plant Resources
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• 제7권2호
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• pp.147-154
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• 2004

Antifreeze proteins (AFPs) accumulate in the leaves of barley during cold acclimation, where they may inhibit ice recrystallization and produce freezing resistance of the plant. Four parental diallel crosses of the barley varieties were used to determine the heritability of AFPs and the relationship between the accumulation level of AFPs and freezing resistance. The concentration of apoplastic proteins in the cold-acclimated leaves was increased in the mean by four-fold over as compared with that of nonacclimated. The diallel cross analyses revealed that the gene of Sacheon 6 was dominant and those of Reno and Dongbori 1 were recessive. The AFPs had high narrow-sense heritabilities. The general combining ability effects of Reno and Dongbori 1 were much higher than the other parents. The bands of 32-kD for GLP, 35-& 28-kD for CLP and 25-, 22- & 16-kD for TLP were observed in the apoplastic extracts from cold-acclimated plants, but there were no clear differences between the parents and Fl hybrids. The concentrations of AFPs were significantly correlated with the degree of freezing resistance, indicating that the concentration of AFPs in the plant is the very important factor for freezing resistance.

• The Plant Pathology Journal
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• 제26권4호
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• pp.421-423
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• 2010

New sources of barley (Hordeum vulgare L.) resistant to spot blotch, caused by Cochliobolus sativus, are needed to provide effective resistance because of the rapid change pathotype patterns of C. sativus in fields. The purposes of our study were to develop a method to screen barley for resistance to spot blotch disease and then use this methodology to screen barley genotypes for resistance to the major virulent pathotype Pt4 in barley populations in Syria. A transparent tape method, in which a conidial suspension of C. sativus was dropped onto transparent tape and placed, treated-side down, on the second leaf surface of barley plants. Disease symptoms of fungus were easily detected on the leaves covered by the transparent tape after 48h of inoculation. The transparent tape method was repeatable and the disease scores obtained were correlated (r = 0.91, P = 0.001) with those obtained by the seedling assay. This method may be beneficial in various plant pathology breeding programs.

• Applied Biological Chemistry
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• 제46권4호
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• pp.333-337
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• 2003

맥엽과 이를 이용한 항산화 조성물을 제조하여 이에 존재하는 nitrite scavenging효과와 이에 따른 발암원으로 부터의 보호 효과를 조사한 결과는 다음과 같다. 맥엽과 항산화 조성물 모두 수층에 비하여 메탄올층에서 아질산염 소거능이 2배 이상 높게 나타나 M-BL50의 0.01 mg/ml 농도에서 약 64.3%, M-AM 0.01 mg/ml 처리군이 이보다 약간 높은 68.3% 정도로 나타내었다. 마우스 단핵구 기원인RAW 264.7(macrophage, mouse)에 맥엽과 항산화 조성물의 수층과 메탄올층을 처리하고 세포 생존율을 측정한 결과 수층 처리군에 비해 메탄올층 처리군에서 모두 세포 생존율이 더 낮게 측정되었으며 모든 시료에서 농도의존적으로 세포 증식이 억제되었다. 특히, M-AM을 처리한 군의 경우 모든 농도에서 가장 낮은 세포 생존율을 나타내어 항산화물 첨가에 의해 세포 증식 억제 효과가 상승됨을 알 수 있었으며 $IC_{50}$ 수치 또한 메탄올층과 A-AM시료 처리군에서 현저히 낮은 수치를 나타내었다. 또한 세포 내 아질산염 소거능을 측정한 결과 역시 마찬가지로 맥엽 입자 크기에 따른 차이는 나타나지 않았으며 수층 처리군에 비해 메탄올층 처리군과 항산화물을 첨가한 군에서 모두 높은 소거능을 나타내어 맥엽 및 기타 항산화물을 섭취함으로써 체내에서의 니트로사민 생성을 억제시킬 수 있을 것으로 예상된다.

• 한국환경과학회지
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• 제8권2호
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• pp.255-261
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• 1999

To investigate the effects of strong acidic electrolytic water on the chloroplast, barley leaves were treated with strong acidic electrolytic water(pH 2.5). And to investigate the effects of weak acidic electrolytic water on the chloroplast development, etiolated barley leaves were treated with weak acidic electrolytic water(pH 6.5) during greening period. Chl contents, Fo, Fv, and Chl fluorescence quenching coefficient in barley leaves were measured during and after treatment of acidic electrolytic water. The following results were obtained. Chl a, b, and carotenoid were decreased with treatment of strong acidic electrolytic water. Chl contents were significantly decreased than that of the control after 5 min. These results provide evidence that the strong acidic electrolytic water dissimilate the Chl and so that the value of Fo was slightly increased. The strong acidic electrolytic water damaged PS II because Fo was increased and Fv, Fm, and Fv/Fm ratio were decreased. qP, qNP and qE were decreased. On the other hand qI was increased than that of the control. But Chl content and Chl fluorescence patterns were a little changed as the pH increase over 4.0 Chl a, b, and carotenoid were increased with treatment of weak acidic electrolytic water during greening period. Chl contents were significantly increased than that of control after 12 hours greening. These results provide evidence that the weak acidic electrolytic water accelerated the chlorophyll synthesis. And the weak acidic electrolytic water accelerated PS II development because Fv, Fm, qP and Fv/Fm ratio were increased than that of the control.

• 한국작물학회지
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• 제46권4호
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• pp.296-302
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• 2001

Winter cereals are acclimated during wintering, and thereafter their freezing resistance is increased. In order to analyze inheritance and heritabilities for photoinhibition of photosynthesis by high light intensity under low temperature, and to evaluate the relationship between low temperature-induced photoinhibition and winter survival, 4 parental half diallel crosses were used. The detached leaves of 7-8cm long from plants grown for 35 and 55 days were placed on wet filter paper and placed in trays at 5$^{\circ}C$ cold room with 1,200 $\mu$mol $m^{-2}$ $s^{-1}$ PPFD. Chlorophyll fluorescence was measured with a chlorophyll fluorescence system after dark adaptation for 30 min. The Fv/Fm of 35day old plants was reduced from 0.714 in the control leaves to 0.409 and 0.368 following photoinhibitory treatment of 6h and 8h and the CVs were increased from 0.8% to 22.2-22.3%. The Fv/Fm of 55-day old plants was reduced from 0.775 in the control leaves to 0.485 and 0.439 following photoinhibitory treatment of 10h and 12h, respectively. According to half diallel cross analysis, Reno and Dongbori 1 (highly resistant to photoinhibition) was dominant, but Oweolbori (susceptible to photoinhibition) was recessive, and photoinhibition showed partial dominance with highly additive gene action. Dongbori 1 showed the greatest GCA effects for photoinhibition, and GCA/SCA ratios (8.7-22.3 times) indicated that the additive variance for the character was more important. Winter survival in barley crosses was positively correlated with resistance to photoinhibition and significantly fitted by linear regression ($R^2$=0.751$^{**}$-0.779$^{**}$). The chlorophyll fluorescence measured by Fv/Fm has been found to be highly inheritable and very useful in evaluating relative levels of freezing resistance in barley.ley.

• 한국식품조리과학회지
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• 제26권4호
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• pp.398-405
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• 2010

In this study, white bread was prepared containing 1.5, 3.0, 4.5 or 6.0% barley leaves tea powder (BLTP). The samples along with a control were then compared regarding their quality characteristics, including pH, total titratable acidity, fermentation power of dough expansion, specific volume, baking loss, moisture content, color, textural characteristics, internal surface appearances and sensory qualities, all to determine the optimal ratio of BLTP. As the BLTP content increased, the pH of the dough and bread and the lightness decreased, whereas the total titratable acidity increased. The fermentation power of dough expansion increased with a longer incubation time. The control group was evidenced by a significantly higher specific volume and baking loss than that observed in the BLTP samples. However, greenness and yellowness evidenced the opposite effect. Hardness was highest at a substitution level of 1.5% and lowest at a level of 4.5%. Fracturability and resilience were not significantly different among the samples. For the internal surface appearance, darkness and greenness increased both increased at higher BLTP content. In the sensory evaluation, color, flavor and overall acceptability were highest in the control bread samples but minimal at a substitution level of 6.0%. Softness was the highest at the 3.0% substitution level and lowest in the control bread samples. Barley leaf flavor, astringency, bitterness and off-flavor increased as the BLTP content increased. Delicious taste was not significantly different among the samples. In conclusion, the results demonstrated that 4.5% BLTP may prove quite useful as a substitute for wheat flour in the production of white bread and may provide favorable nutritional and functional properties.

• Plant Resources
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• 제4권1호
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• pp.41-47
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• 2001

This study was conducted to investigate the occurrence of Soil borne wheat mosaic virus(SbWMV) in barley fields in Korea and to examine the host pathogenicity of SbWMV. By using the ELISA test, SbWMV was detected in the six regions : Suwon, Milyang, Jinju, Youngkwang, Iksan, and Chonju. SbWMV was isolated from the two strains, Albori strain from Jinju and Eunpamil strain from Milyang. SbWMV was collected from leaves showing mosaic, yellowing and necrosis stripes. SbWMV was inoculated mechanically on 1∼1.5 leaf stages with leaf-rubbing to identify the host pathogenicity of 36 Korean barley cultivars, a wheat cultivar, two rye cultivars, three Japanese barley cultivars and Chenopodium amaranticola. Viral sympoms of inoculated leaves appeared on moulted loaves about 4 to 6 weeks of inoculation. Baegdong and Tapgolbori, infected from Albori strain and Eunpamil strain infected from Samdobori showed much higher susceptibility than C. amaranticola and C. quinoa which showed ring spots and chlorotic spots respectively. Virus particles were observed by the electron microscope. They were rod-shapes, which are bipartite, of 142 nm or 281 nm in length with 20 nm diameter on infected leaves. Specific detection and identification of SbWMV was set up using the RT-PCR. PCR fragments of SbWMV(0.5kb) were obtained by using the designed primers for SbWMV RNA 2.

• 한국식물병리학회지
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• 제1권1호
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• pp.79-84
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• 1985

근 동질유전자 보리계통에서 보리흰가루병에 대한 유도저항성의 몇가지 특성이 평가되었다. 열처리하여 살생시킨 비친화성균의 포자를 보리 1계통에 전접종한 후 경시적으로 전접종원 제거한 다음 동일부위에 친화성균을 후접종하였을 때 저항성은 유도되지 않았다. 그러나 살상된 전접종균을 제거하지 않고 친화성균을 후접종하였을 경우는 친화성균에 의한 균총형성이 $87.1\~91.2\%$까지 감소하였다. 감염이 이루어지기 전에 전접종했던 비친화성균을 제거하고 친화성균을 후접종하였을 경우에는 친화성균에 의한 균총형성의 감소만이 일어났으나 감염이 이루어진 후에 비친화성균을 제거하고 친화성균을 후접종한 경우에는 친화성균의 균총형성감소는 물론, 형성된 균총의 병반형이 모두 변하였고, 그 중 몇몇은 균총주위에 갈색반점이 형성되었다. 친화성균 혹은 비친화성균을 보리 1차전개엽의 중앙에만 전접종한 후 다시 잎의 전면에 경시적으로 친화성균을 후접종하였을 경우 중앙부위인접상단과 인접하단에서 후접종원에 의해 형성된 균총의 수는 대조구와 차이가 없었다. 그러나 인접사단에서는 형성된 친화성균총의 병반형이 변하지 않았으나 인접상단에서는 균총의 병반형이 약간 변화하였고 이들 균총들에 의해 형성된 포자의 수는 대조구보다 현저히 감소하였다.