• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.2
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• pp.148-153
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• 2004

The Hessian fly, Mayetiola destructor (Say), is known to be one of the major insect herbivores of wheat worldwide. In order to provide molecular events on interactions of the NIL with H21 and larvae of Hessian fly biotype L, the TaCR1 gene, Triticum aestivum cytokinin repressed 1, was isolated through the suppression subtractive hybridization, which was constructed using stems of the NIL with H21 at 6 days after infestation as tester and stems of the recurrent parent Coker797 without H21 at 6 days after infestation as driver. Transcript levels of TaCR1 mRNA in the NIL with H21 were highest at 6 days after infestation but in the Coker797 without H21 until 8 days were similar with those of non-infested plants. Expression of the TaCR1 gene was decreased at early time and then recovered after wounding or $H_2O$$_2$ treatment as well as 6-BAP treatment. Transcripts levels of the TaCR1 gene was changed after MeJA, SA, ethephone, or ABA treatment. In drought treatment, the TaCRl gene were increased at early stage of stress and then decreased at late stage. Expression of the TaCRl gene was continued to decrease through 24 h in the cold treatment. Although the TaCRl gene is increased through infestation in NIL with H21, further study was required to elucidate a role on resistance against larvae of Hessian fly. However, the TaCR1 gene could be used as marker gene on response of plants against abiotic stresses as well as application of plants with several hormones.

• Journal of Plant Biotechnology
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• v.38 no.3
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• pp.209-214
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• 2011

Transgenic lettuce plants were successfully obtained from hypocotyl explants inoculated with Agrobacterium tumefaciens, which harbored a binary vector plasmid with Bcl-2 gene, related to apoptosis. After culture and selection on MS medium a number of kanamycin-resistant plantlets were regenerated. Polymerase chain reaction, Southern blot analysis and Northern blot analysis were used to identify and characterize the transgenic plants with the integrated Bcl-2 gene. Over 100 transgenic plants have been established in soil and flowered in the greenhouse. T1 progeny of 100 transgenic lettuce inbred lines were inoculated with Sclerotinia sclerotiorum. Expression of the Bcl-2 peptide in transgenic lettuce plants provides high levels of field resistance against Sclerotinia sclerotiorum, causal agent of the agronomically important fungal disease of lettuce.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.26-39
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• 2020

In maize, immature embryos (IEs) are highly regenerative explants most suitable for producing high frequencies of plantlet regeneration in vitro. Apart from media, explants, and hormones, genotypic variation also influences in vitro characters to a great extent. In the present study, IEs were used to study the distinctive effect of variation of size/stage and hormones in different genotypes on five in vitro characters viz., frequency of callus induction, growth rate of total callus, frequency of E. callus induction, and volume and number of regenerated plantlets. LS medium with different concentrations of 2,4-D (0.5, 1.5, 2.5, 4.0 and 5.0 mg/L) were used to study the former four in vitro characters, and medium with 6-benzylaminopurine and kinetin (0.5 mg/L, each) was used for plantlet regeneration. IEs of 1.0, 1.5, 2.0, 2.5 and 3.0 mm in size were isolated from four inbred lines viz., NM74C, NM81A, NM5883 and NM5884. Two-way ANOVA revealed that explant size and genotypes, as well as hormonal concentrations showed significant effects on in vitro characters. Two millimeter IEs were found to be suitable for in vitro cultures. LS medium with 1.5 mg/L 2,4-D and LS with BAP and Kn (0.5 mg/L, each) were found to be the best hormonal concentrations for callus induction, maintenance, and regeneration, respectively. Among the four genotypes, NM81A and NM5883 yielded more non-embryogenic and Type I E. calli. In contrast, NM74C and NM5884 yielded more highly regenerative Type II calli. Inbred line NM5884 was found to be the best among these four genotypes.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.39-44
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• 2010

An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, $0.5mgL^{-1}$ 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins ${\alpha}-napthalene$ acetic acid (NAA), indole acetic acid (IAA) and ${\beta}-indolebutyric$ acid (IBA) (0.5 and $1.0mgL^{-1}$). The $1.0mgL^{-1}$ IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.583-583
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• 2016

다양한 평가지표가 반영된 복합 지수(Composite Index)는 물관리 정책의 우선순위 결정 및 정책성과의 모니터링에 유용한 도구로 사용되고 있다. 각 지표별 중요도를 나타내는 가중치는 최종 지수의 산정에 영향을 미칠 수 있으며, 그 결정방법도 Data Envelopment Analysis(DEA), Benefit of doubt Approach(BOD), Unobserved Component Model(UCM), Budget Allocation Process(BAP), Analytic Hierarchy Process(AHP), Conjoint Analysis(CA) 등 다양하다. 본 연구에서는 여러 가지 가중치 결정방법 중 통계적 방법인 주성분 분석(Principal Component Analysis, PCA)을 사용하여 Park et al.(2016)이 제시한 물관리 탄력성 지수(Water Management Resilience Index, WMRI)에 대한 가중치를 산정하여 동일 가중치를 적용한 기존 결과와 비교하였다. 물관리 탄력성 지수는 자연조건상 물관리 취약성(Vulnerability), 기존 수자원 인프라의 견고성(Robustness), 물위기 적응전략의 다양성(Redundancy)의 3가지 부지수(sub-index)는 각각 13개, 11개, 7개의 지표(Indicator)로 구성되어 있으며, 117개 중권역을 다목적댐 하류 본류유역(범주 1), 용수공급 및 유량조절이 불가능한 지류(범주 2)와 가능한 지류(범주 3)로 분류하여 적용되었다. 각 부지수별로 추출된 3개, 5개, 3개의 주성분이 전체 자료의 76.4%, 71.2%, 63.2%를 설명하는 것으로 분석되었으며 부지수별 주성분의 고유벡터(Eigenvector)와 고유값(Eigenvalue)를 계산하고 각 지표의 가중치를 산정하였다. 주성분 분석에 의한 가중치와 동일 가중치를 적용하였을 경우와 비교해보면 취약성 부지수 1.9%, 견고성 부지수 1.9%, 다양성 부지수 2.1%의 차이가 나타나며 물관리 탄력성 지수는 0.4%의 차이를 보임에 따라 Park et al.이 제시한 연구결과의 적정성을 확인할 수 있었다. 주성분 분석은 객관적인 가중치 설정을 위한 통계적 접근방법의 하나로써 다양한 물관리 정책지수 산정시 활용될 수 있을 것이며, 향후 다른 가중치 산정방법을 적용함으로써 각 방법에 따른 지수 결과의 민감도 및 장단점을 분석할 수 있을 것으로 판단된다.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.31 no.4
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• pp.687-699
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• 2021

When a vulnerability occurs in a program, it is documented and published through CVE. However, some vulnerabilities do not disclose the details of the vulnerability and in many cases the source code is not published. In the absence of such information, in order to find a vulnerability, you must find the vulnerability at the binary level. This paper aims to find out-of-bounds read vulnerability that occur very frequently among vulnerability. In this paper, we design a memory area using memory access information appearing in binary code. Out-of-bounds Read vulnerability is detected through the designed memory structure. The proposed tool showed better in code coverage and detection efficiency than the existing tools.

• Journal of Ecology and Environment
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• v.46 no.3
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• pp.218-242
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• 2022

Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA₃), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.65.2-65
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• 2003

To protect the plant against several soil-borne pathogens, we are currently constructing disease-resistant transgenic root stock for the growth of cucurbitaceae vegetable plants, watermelon and gourd. We made a watermelon cDNA library from Cladosporium cucumerinum-Infected leaves for substractive hybriazation and differential screening. We isolated the several pathogen inducible cDNA clones, such as caffeoyl-CoA-methyltransferase, LAA induced protein, receptor-like kinase homolog, hydroxyproline-rich glycoprotein, catalase, calmodulin binding protein, mitochondrial ATPase beta subunit, methyl tRNA synthetase and WRKY transcription factors. We previously obtained CaMADS in pepper and galactinol synthase ( CsGolS) in cucumber that were confirmed to be related with disease-resistance. CaMADS and CsGolS2 were transformed into the inbred line 'GO701-2' gourd, the inbred line '6-2-2' watermelon and the Kong-dye watermelon by Agrobacterium tumerfaciens LBA4404. Plant growth regulators (zeatin, BAP and IAA) were used for shoot regeneration and root induction for optimal condition. Putative transgenic plants were selected in medium containing 100mg/L kanamycin and integration of the CaMADS and CsGO/S2 into the genomic DNA were demonstrated by the PCR analysis. We isolated major soil-borne pathogens, such as Monosporascus cannonballus, Didymella bryoniae, Cladosporium cuvumerinum from the cultivation area of watermelon or root stock, and successfully established artificial inoculation method for each pathogen. This work was supported by a grant from BioGreen 21 program, Rural Development Administration, Republic of Korea.

• Journal of Society of Preventive Korean Medicine
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• v.26 no.3
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• pp.97-108
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• 2022

Objectives : The purpose of this study is to confirm the efficacy and safety of Gongjin-dan and Ssanghwatang for chronic fatigue. Methods : A total of 90 people, between 19 and 65 years old, will be recruited to participate in a randomized, double-blind, and placebo-controlled a clinical trial. Participants in the Gongjin-dan group will take one pill of Gongjin-dan along with three packs of placebo oral liquid Ssanghwa-tang per day for 4 weeks. Participants in the Ssanghwa-tang group will take three packages of liquid Ssanghwa-tang and one placebo Gongjindan pill per day for 4 weeks. In the placebo group, participants will take one pill of placebo Gongjin-dan and three packs of placebo liquid Ssanghwa-tang per day, for 4 weeks. Outcomes will be measured at the baseline, 4^th week, and 6^th week. The primary outcome is the change in the Fatigue Severity Scale (FSS). Secondary outcomes are the change of Multidimensional Fatigue Inventory-20 (MFI-20), Chalder Fatigue Scale (CFQ), Short-Form 36 Health Survey (SF-36), Korean Version of Schedule of Fatigue and Anergy/General Physician (SOFA/GP), Glucose, Lactate, Ammonia, Free Fatty Acid (FAA), d-ROMs&BAP, Selenium, and Cortisol. Results : This trial was approved by the institutional review board of Pusan National University Korean Medicine Hospital (registry number: PNUKHIRB 2021-10-005). Recruitment opened in November 2021 and is supposed to be completed by December 2022. Conclusions : This trial will provide clinical information to determine the efficacy and safety of Gongjindan and Ssanghwa-tang for chronic fatigue.

• Journal of the Korea Society for Simulation
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• v.18 no.2
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• pp.19-27
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• 2009

This paper addresses the berth allocation problem in naval ports. Navy vessels need various services such as emergency repair, missile loading, oil supply and many others while commercial vessels only unload and load container at the port. Furthermore, naval vessels have to shift frequently due to a limited capacity of the port. The objective of this paper is to minimize the total number of nesting vessels at the naval port. In other word, the objective is to maximize the total number of naval battleships engaging in the sea. A mixed integer programming(MIP) model is developed and experiments are conducted with ILOG CPLEX 11.0. We compare the computational results of the MIP model to the current scheduling approach by the ROK Navy. The results showed that MIP model performed well by minimizing the number of nesting vessels. and avoiding unnecessary shifts.