• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.6
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• pp.585-590
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• 2000

A hemolysin producing strain was isolated from Kum rivet estuary located in west part of Korea. In the process of identification the isolated strain was similar to V. mimicus but did not show characteristics of known Vibrio species; therefore, the strain was designated as Vibrio sp. D9 ( V. kumkang) tentatively and further identification study was carried out by comparing its bacteriological characteristics, Morphologically Vibrio sp. D9 was a typical straight roe with a polar flagellium. Among known Vibrio species no identical strains were found when using automatic bacteria identification system ($MicioLog^(TM)$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp. D9 showed 18 and 13 different responses as compared to V. mimicus and V. cholerae, respectively. Clear hemolysis zones were observed with the strain against human and sheep blood agar plate, Hemolytic toxicity was confirmed by strong vascular permeability and fatal toxicity against mouse was also observed. Thus the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. D9 were salinity of $0{\~}5.0{\%}$, pH of $6.4{\~}9.8$, temperature of $15{\~}41^{\circ}C$, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.622-629
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• 2011

We investigated the effect of the discharged water from Bong Stream, which is located in the drainage area of Gangjinman area on the bacteriological water quality in the coastal area after rainfall events. Following 12.5 mm of rainfall, water discharged from Bong Stream had a very limited effect on bacteriological water quality in adjacent area and the affected area did not extend to the designated area. On the other hand, after 23 mm rainfall, the density of fecal coliform at stations located in the designated area was higher than at stations located in the adjacent area. The degree of bacteriological contamination at the surveyed stations in the coastal area after rainfall events did not show a relationship with distance from the shoreline. These results indicate that the direction of spread and the range of contaminants from the drainage area were affected by tides at the time of the survey. Therefore, a detailed survey of the effects of tides on the diffusion characteristics of the contaminants from Bong Stream is needed to establish a proper management plan for the surveyed area.

• Plant Disease and Agriculture
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• v.5 no.2
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• pp.69-76
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• 1999

Acidovorax avenae subsp. avenae is the causal pathogen of several hosts including oats corn foxtail millet wheatgrass sugarcane and rice. The pathogen is a seedborne pathogen of rice and known to occur widely in rice growing countries. The pathogen cause inhibition of germination brown stripe on the leaf curling of the leaf sheath and abnormal elongation of the mesocotyl of irce. Bacterial colonies grow slowly and are convex circular and creamy with tan to brown center. The causal baterium is Gram-negative and rod shape with a single polar flagellum Nonfluorescence poly-$\beta$-hydroxybutyrate accumulation and precipitate formation around the colony on the medium are useful in the differentiation of this bacterium from other subspecies of A. avenae as well as nonfluorescent bacteria pathogenic to rice. This bacterium has belonged to the genus of Psdeudomonas but recently was transferred to the new genus Acidovorax on the basis of bacteriological and molecular biological data. However the difference of biochemical characteristics protein profile of the cell and host range among strains should be more clarified. To develop an effective control strategy for this disease understanding of detailed life cycle of the disease ritical environmental factors affecting disease development on each host and relationship to grain discoloration of rice are prerequisite. Although the affected area has been world-widely reported there is on recent progress on the understanding of the bacteriological and ecological characteristics of the causal bacterium and control means of the disease.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.5
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• pp.414-422
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• 2012

This study evaluated the effect of the inland pollution sources on the bacteriological water quality in Narodo area after rainfall events. Following 16 mm of rainfall, the water discharged from Dohwa Stream and contaminants from Balpo village had a very limited effect on bacteriological water quality in adjacent area and the affected area did not extend to the designated area. In comparion, after 33 mm of rainfall, the density of fecal coliforms at stations located in the mouth of Dohwa stream and the discharg point of contamination sources located in Balpo village were lower than after 16 mm of rainfall. These results were obtained during an extreme high tide near the coast the contaminants discharged from Dohwa stream and Balpo village. Therefore, it is necessary to conduct a detailed survey to examine the dffects of tides on the diffusion characteristics of the contaminants discharged from Dohwa stream and Balpo village in order to manage of Narodo area efficiently.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.545-550
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• 2002

A hemolysin producing bacterial strain which belong to Vibrio species was isolated from the Kum River estuary. In the process of identification, the strain did not show characteristics of known Vibrio species; thus, the strain was designated as Vibrio sp, E10 (V. kunsan) tentatively and further identification study was carried out by comparing its bacteriological characteristics. Morphologically Vibrio sp, E10 was comma shaped rod with a polar flagellium. Clear hemolysis zones were observed with the strain against human and sheep blood agar. Hemollytic toxicity was confirmed by strong vascular Permeability and fatal toxicity against mouse was also observed. Therefore the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. E10 were ranged salinity of 0$\~$$4.5\%$, pH of 6.2$\~$9.2, temperature of 14$\~$42$^{\circ}C$, respectively, 16S rDNA partial sequence of Vibrio sp, E10 showed $99\%$ homology with dozens of V. cholerae species including V, cholerae El Tor N16961 and V, snmisnfus ATCC 33653T. This strain belonged to Proteobacteria; gamma subdivision; Vibrionacea: Vibrio. But, among knorn Vibrio species no identical styains were found when using automatic bacteria identification system ($MicroLog^{TM}$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp, E10 showed 18 and 11 different responses as compared to V. mimicus and V, cholerae, respectively.

• Korean Journal of Veterinary Research
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• v.31 no.4
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• pp.457-469
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• 1991

Clinical and bacteriological observations on infectious atrophic rhinitis of swine were conducted in order to obtain some basic information for the clinical and immnnological control of the disease prevailing in the republic. Samples were collected from nasal cavities of 135 4~12 week old pigs from 12 herds and from turbinates of 199 slaughtering pigs from 14 swine herds to investigate the prevalence of Bordetella bronchiseptica and/or Pasteurella multocida in the nasal cavities of the pigs. On the examination of nasal swabs by cultural techniques and of gross lesion of snouts of slaughtering pigs, all the swine herds investigated were found to be affected by atrophic rhinitis and a total of 84 B bronchiseptica and 139 P multocida cultures were isolated from the nasal cavities of the pigs. Of the 199 slaughtering pigs, some 48% revealed gross pathological lesion typical of atrophic rhinitis and the prevalence of B bronchiseptica and P multocida were 27.6% and 46.7%, respectively. Biochemical properties, antimicrobial susceptibilities, serological characteristics and toxigenicity of the isolates of B bronchiseptica and P multocida were investigated.

• Membrane and Water Treatment
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• v.9 no.3
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• pp.181-188
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• 2018

A sidestream contains the filtrate or concentrate from the belt filter press, filter backwash and supernatant from sludge digesters. The sidestream flow, which heads back into the sewage treatment train, is about 1-3% less than the influent flow. However, the sidestream can increase the nutrient load since it contains high concentrations of phosphorus and nitrogen. In this study, the removal of PO4-P with organic matter characteristics and bacteriological changes during the sidestream treatment via ladle furnace (LF) slag was investigated. The sidestream used in this study consisted of 11-14% PO4-P and 3.2-3.6% soluble chemical oxygen demand in influent loading rates. LF slag, which had a relatively high $Ca^{2+}$ release compared to other slags, was used to remove $PO_4-P$ from the sidestream. The phosphate removal rates increased as the slag particle size decreased 19.1% (2.0-4.0 mm, 25.2% (1.0-2.0 mm) and 79.9% (0.5-1.0 mm). The removal rates of dissolved organic carbon, soluble chemical oxygen demand, color and aromatic organic matter ($UV_{254}$) were 17.6, 41.7, 90.2 and 77.3%, respectively. Fluorescence excitation-emission matrices and liquid chromatography-organic carbon detection demonstrated that the sidestream treatment via LF slag was effective in the removal of biopolymers. However, the removal of dissolved organic matter was not significant during the treatment. The intact bacterial biomass decreased from $1.64{\times}10^8cells/mL$ to $1.05{\times}10^8cells/mL$. The use of LF slag was effective for the removal of phosphate and the removal efficiency of phosphate was greater than 80% for up to 100 bed volumes.

• Korean Journal of Microbiology
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• v.23 no.3
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• pp.223-229
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• 1985

We have previously isolated seven strains of spiral form bacteria, which have shown several similar characteristics to Leptospira from patient with epidemic pulmonary hemorrhagic fever and matural paddy water. Further studies have performed to characterize them. All of the isolated bacteria were aerobic, gram negative and spiral form and their growth were completely inhibited at temperature below $13^{\circ}C\;and\;50^{\circ}C$. In sensitivity test to drugs, the bacteria were resistant to the bile salts, 5-Fu and Amphotericine B, but sensitive to the 8-Azaguanine. The isolated bacteria as well as 5 serotypes of Leptospira interrogans reacted the patient sera and it was also shown that antisera from rabbit immunized with the isolated bacteria reacted with 5 serotypes of Leptospira interrogans. These results suggest that the isolated bacteria may belong to the genus Leptospira.

• Research in Plant Disease
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• v.7 no.3
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• pp.145-149
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• 2001

A pathogenic bacterium was isolated from galls on Wistaria floibunda at Jinryang of Kyungsan, Kyungbuk, Korea in May, 2000. This bacterium was cultured and tested for pathogenicity on the host. Also its morphological and physiological characteristics were examined. Inoculation with the bacterium isolated from the gall caused the same symptoms as those under natural conditions. On the basis of bacteriological characteristics and pathogenecity on the host plant of the organism, the causal bacterium was identified as Pantoea agglomerans pv. milletiae. This is the first report of this bacterium to occur on W. floribunda plant in Korea. Therefore we proposed to name the disease as "bacterial gall of Wistaria floribunda" by P. agglomerans pv. milletiae.milletiae.

• Journal of Food Hygiene and Safety
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• v.30 no.4
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• pp.350-358
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• 2015

This study was designed to evaluate the sanitary characteristics of sea water in a part of the south coast, Korea and to check the seawater which is in compliance with the recommended bacteriological criteria for shellfish cultivation. The samples of sea water were collected at 14 sampling stations established in the survey area between March 2014 and October 2014. Food poisoning caused by seafood consumption is often associated with pathogenic microorganisms originated from fecal contamination. Therefore, fecal coliform is very important criteria for evaluating the safety of fisheries in coastal areas. The range of geometric mean (GM) and the estimated 90th percentile values of total coliform were 4.1~83.1 MPN/100 mL, and 11.7~834.1 MPN/100 mL, respectively. The GM and the estimated 90th percentile values of fecal coliform were 2.5~22.7 MPN/100 mL and 2.5~170.0 MPN/100 mL, respectively. Therefore, the bacteriological safety of seawater at this shellfish-growing area met with the Korean Shellfish Sanitation Program (KSSP) criteria for a growing area. However, the values are seasonally exceed the KSSP criteria, suggesting that the monitoring and evaluation of seawater quality is very important in shellfish-growing area.