• 제목/요약/키워드: bacterial isolation

검색결과 600건 처리시간 0.032초

Screening and Isolation of Antibiotics Resistance Inhibitors from Herb Materials. V.- Resistance Inhibition by Acorenone from Acorus gramineus Solander

  • Kim, Hye-Kyung;Moon, Kyung-Ho;Lee, Chung-Kyu
    • Natural Product Sciences
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    • 제6권1호
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    • pp.36-39
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    • 2000
  • Acorenone, a diterpene isolated from Acorus gramineus, showed strong resistance inhibitory activity against multi-drug resistant microorganisms such as Staphylococcus aureus SA2, which has resistance to 10 usual antibiotics including chloramphenicol (Cm). At the level of $5\;{\mu}g/ml$ when combined with $50\;{\mu}g/ml$ of Cm. Bacterial resistance to Cm is due to the presence in resistant bacteria of an enzyme, chloramphenicol acetyltransferase (CAT), which catalyses the acetyl-CoA dependent acetylation of the antibiotic at C-3 hydroxyl group. To elucidate the mechanism of resistant inhibitory effect, the acorenone which had the strongest resistant inhibitory activity, was investigated on the CAT assay. As the result, the combination of Cm and acorenone showed the strongest inhibitory activity on CAT as noncompetitive and dose dependent manner.

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Isolation and Identification of Myxobacteria KR025 and Searching of Their Bioactive Compounds (점액세균 KR025의 분리 동정 및 생리활성물질의 탐색)

  • 김병섭;안종웅;조광연
    • Korean Journal Plant Pathology
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    • 제14권4호
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    • pp.345-349
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    • 1998
  • Fifty isolates of myxobacteria were isolated from soils from several areas in Korea during 1996-1997 and bioactivity against plant pathogenic fungi of these isolates was examined. A myxobacterial isolate KR025 showed good antifungal activities against Pyricularia oryzae, Cryphonectria parasitica, Colletotrichum lagenarium, and C. gloeosporioides but did not against Rhizoctonia solani, Fusarium oxysporum and Pythium ultimum. The bacterium was identified as Myxococcus fulvus based on morphological and physiological characteristics. Antifungal substances were extracted from culture broth and bacterial cell of Myxococcus fulvus KR025 by ethyl acetate. Antifungal substance of Myxothiazole (100 ${\mu}{\textrm}{m}$/ml) produced by Myxococcus fulvus KR 025 controlled 97.0% rice blast, tomato late blight, wheat leaf rust, and barley powdery mildew and showed 45.0 and 82.6% disease control of rice sheath blight and cucumber gray model, respectively.

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Value Addition Span of Silkworm Cocoon - Time for Utility Optimization

  • Reddy, R. Manohar
    • International Journal of Industrial Entomology and Biomaterials
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    • 제17권1호
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    • pp.109-113
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    • 2008
  • Realizing the scope of utilizing by-products of silk cocoons by applying appropriate methods is the immediate crave to optimize returns. The nutritive value of pupae suits for human diet, feed for poultry, carps, fish, rabbits, piggery and dogs. The pupal skin, fat, oil, cocoon palade have applications in oleo chemical, soap, glycerin, cosmetic, artificial fibres, membranes and n-triacontanol isolation. The pupal proteins Chitin, Shinki fibroin, Serrapeptidase, glucosamine are latent precursors of post surgical, anti-carcinogenic, anti-inflammative, anti-bacterial, anti-histaminic, gastric, hepatitis, pancreatitis, leukocytopenia, neurological, ophthalmic, blood pressure, cardiac and diabetic medicines and for preparation of vitamins A, E and K. The silk and its proteins sericin and fibroin are potentially used for wound healing, diabetes, impotence, sinusitis, arthritis, edema, cystitis, epididymitis, tissue regeneration, cancer, post-surgical trauma and used as anti-oxidatives, bio-adhesives, ultra violet screens and bio-active textiles. The waste cocoons can be used in making art crafts like garlands, carpets, overcoats, decoratives and greeting cards. The in-depth research towards utility optimization and make aware this reality to sericulturists, reelers, weavers, traders, entrepreneurs, policy makers etc., is the upright want of the today's Sericulture industry.

Isolation of Cadmium-tolerant Bacteria and Accumulation of Cadmium into the Bacterial Cell (카드뮴의 내성균분리 및 균체내 축적)

  • 김영배;이수래
    • Microbiology and Biotechnology Letters
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    • 제4권3호
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    • pp.111-115
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    • 1976
  • A strain of Enterobacter cloacae isolated from soil showed the evidence of growth in medium containing 1,500 ppm of Cd$\^$++/ though its growth was inhibited at high cadmium concentrations. This strain accumulated 59% of cadmium into the cells during incubation in medium containing 0.5 ppm of cadmium and its uptake was nearly proportional to the dry weight of cells, the average being 7.8 mg cadmium per g dry cells. It was also found that 60-70% of cadmium accumulated in cells were distributed in the cell wall fraction.

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Isolation and Characterization of Chitosanase-Producing Microorganism, Aureobacterium sp. YL, from Crab Shells

  • Lee, Dong-Mi;Lee, Ei-Leen;Lee, Kang-Man
    • Journal of Microbiology and Biotechnology
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    • 제10권2호
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    • pp.208-214
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    • 2000
  • A bacterial strain producing extracellular chitosanase was isolated from crab shells and identified as a member of the genus Aureobacterium The production of chitosanase was proportionally related to the microbial growth, induced by the presence of chitosan, and repressed by glucose at 0.5% (w/v) concentration or higher. The optimal culture conditions for the production of chitosase were 3$0^{\circ}C$ and pH 7.0. Among the nitrogen sources tested, incubation with 0.25% (w/v) concentrations of tryptone and casitione showed the best production of chitosanase. The chitosanase of Aureobacterium sp. YL produced chitobiose as a major product and glucosamine, chitotriose, chitotetraose, and chitopentaose as minor products from chitosan.

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Isolation and Identification of Bacteria Producing a Soybean Milk Clotting Enzyme (두유 응고효소 생산균의 분리 및 동정)

  • 하덕모;이철우
    • Microbiology and Biotechnology Letters
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    • 제17권2호
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    • pp.109-114
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    • 1989
  • Seventeen bacterial strains producing an extracellular soybean milk clotting enzyme were Isolated from 150 soil samples, and identified as Bacillus cereus(8 strains), Bacillus pumilus(8 strains) and Bacillus licheniformis (1 strain). Among them, Bacillus pumilus strain 118 and Bacillus licheniformis strain 192 showed relatively high soybean milk clotting activity. The coagulability of enzymes from these strains decreased as the pH of soybean milk was increased from 6.0 to 7.0. The optimum temperature for soybean milk clotting activity was $65^{\circ}C$.

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Taxonomic Studies of the Beta Hemolysis-causing Pathogen Bacillus cereus Isolated from Sea Water

  • Kim, Sam-Sun;Park, Yong-Ha;Lee, Jung-Sook;Yoon, Jung-Hoon;Shin, Yong-Kook;Rhee, In-Koo;Kim, Young-Jae
    • Journal of Microbiology and Biotechnology
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    • 제8권1호
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    • pp.67-73
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    • 1998
  • A bacterial strain that excretes hemolysins and proteases into the growth medium was isolated from sea water and designated as KYJ 961. A nearly complete nucleotide sequence of a 16S ribosomal RNA gene from the isolate was determined following the isolation and cloning of amplified genes. On the basis of the 16S ribosomal DNA sequence data, and morphological, chemotaxonomic, and physiological characteristics, strain KYJ 961 was classified as a strain of Bacillus cereus.

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Isolation and Cultivation of Microorganism Producing Levanbiose from Levan (Levan으로부터 Levanbiose를 생산하는 미생물의 분리 및 배양)

  • 이태호;강은정;강수경
    • Journal of the Korean Society of Food Science and Nutrition
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    • 제27권3호
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    • pp.441-447
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    • 1998
  • A bacterial strain No. 43 was isolated from soil samples as a levan-assimiating microorganism producing an extracellular levanase and hydrolying levan to levanbiose. According to the taxonomic characteristics of its morphological and physiological properties, the strain was idenified as Pseudomonas sp. No. 43. The optimum culura medium was composed of 10g levan, 5g(NH4)2SO4, 3g NH4Cl, 3g polypepton, 1g K2HPO4, 0.5gMgSO4.7H2O, and 0.2g MnCl2.4H2O per liter. The cultivation for levanase was carried out in pH 7.0 at 4$0^{\circ}C$ for 28hr. The reaction product was a kind of oligosaccharide and it was purified by chilled ethanol precipitation and gel filtration for evalluation of degree of polymerization (DP). The purified product was determined as levanbiose of MW 342 and DP2 by HPLC and FAB-MS.

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Isolation and Characterization of Pseudomonas sp. T-1 Degrading Terephthalic Acid (Terphthalic Acid를 분해한는 Pseudomonas sp. T-1의 분리 및 특성)

  • 서승교
    • Journal of Environmental Health Sciences
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    • 제21권4호
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    • pp.44-48
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    • 1995
  • 26 bacterial strains capable of growing on Terephthalic acid (TPA) in minimal medium were isolated from soil and wastewater by selective enrichment culture, and among them, one isolate which was the best in the cell growth and TPA degradation was selected and identified as Pseudomonas sp. T-1 by its characteristics. Cell growth almost revealed a stationary phase at 24 hrs after cultivation. Cell growth dramatically increased in a minimal medium containing 0.1% of TPA as a sole carbon source and TPA was not detected any more at 80 hrs after cultivation. Therefore, it is suggested that Pseudomonas sp. T-1 could be effectively used for the biological treatment of wastewater containing TPA.

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Isolation of Plasmid Korean Copper-Resistant Xanthomonas campestris pv. vesicatoria (한국에서 분리한 고추 더뎅이병균의 구리저항성 Plasmid)

  • 박의훈;조용섭
    • Korean Journal Plant Pathology
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    • 제12권2호
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    • pp.156-161
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    • 1996
  • 세계적으로 구리약제에 대해서 저항성을 나타내는 균주들이 발견되있으며, 이들은 구리약제의 방제효과를 감소시켰다. 국내에서도, 구리 저항성 균주 Xanthomonas campestris. pv. vesicatoria HN94-2, -3, -4, -5, -6 들이 천안지역의 고추재배지에서 처음으로 분리되었으며, 이들 균주들의 nutrient agar에서 황산구리(CuSo\ulcorner)에 대한 최소억제농도(minimum inhibitory concentration, MIC)는 1.4~1.6 mM이었다. 이들은 모두 황산아연(ZnSO\ulcorner)에 대해서는 감수성을 보여, 구리저항성 균주에 대한 방제약제로서 아연 함유 약제를 사용할 수 있을 것이다. 분리된 균주중 HN94-2와 HN94-6을 이용하여 접합(conjugation)을 통해 구리저항성의 전파를 실험한 결과, 이들 두 균주 모두 구리감수성 균주에게 4.3$\times$10\ulcorner에서 1.0$\times$10\ulcorner(transconjugant/donor)의 정도로 구리 저항성이 전이되었다. 이들 HN94-2와 HN94-6 균주의 구리정항성 유전자들은 약 200 kb 정도의 커다란 플라스미드(plasmid)에 존재하며, 이들은 각각 pXVK9402와 pXVK9406이라 명명되었다.

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