• Title/Summary/Keyword: background surface

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Identification of a Gene Required for Gliding Motility in Myxococcus xanthus

  • Lee Cha-Yul;Chung Jin-Woo;Kim Ji-Hoon;Cho Kyung-Yun
    • Journal of Microbiology and Biotechnology
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    • v.16 no.5
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    • pp.771-777
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    • 2006
  • A novel gene (agiA) required for adventurous gliding motility in Myxococcus xanthus has been identified. Null mutations in this gene caused defects in the gliding movement of isolated cells, suggesting that it belongs to one of the A-motility genes. The isolated agiA mutant cells neither glided nor produced slime trails on agar surface. However, agiA was different from other known A-motility genes in that the agiA mutant created in the $S^-$ mutant background glided in the swarm of cells, since other known A-motility mutants created in the $S^-$ mutant background do not move in the swarm of cells. The agiA mutant was also defective in fruiting body development. Sequence analysis predicted that agiA encodes a 787-amino-acid protein with eight tripeptide repeat motifs.

Comparison test for PD measuring about UHF-sensor and HFCT-sensor in cable joint-box of MV grade (MV급 케이블 중간접속부의 PD 측정을 위한 UHF-센서와 HFCT-센서의 비교시험)

  • Shin, Dong-Hoon;Lee, Yong-Sung;Kim, Jung-Yoon;Yang, Jong-Seok;Park, Noh-Joon;Park, Dae-Hee
    • Proceedings of the KIEE Conference
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    • 2006.07c
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    • pp.1511-1512
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    • 2006
  • In this paper, we measured PD signals from artificial surface defects and corona discharges under laboratory circumstances like the fields using both HFCT-sensor and UHF-sensor for MV grade XLPE cable with AMP, spectrum analyzer and oscilloscope. Also, detected signals were analyzed via PRPDA system in this paper. As a result, in this case of HFCT-sensor, the amount of background noise are larger than as compared to UHF-sensor. There is needed some hardware or software or software algorithm removing background noise effectively for diagnosis the underground power transmission cable in the case of HFCT-sensor.

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Generation and characterization of 1H8 monoclonal antibody against human bone marrow stromal cells

  • Kang, Hyung Sik;Choi, Inpyo
    • IMMUNE NETWORK
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    • v.1 no.1
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    • pp.14-25
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    • 2001
  • Background: Bone marrow stromal cells (BMSCs) express many cell surface molecules, which regulate the proliferation and differentiation of immune cells within the bone marrow. Methods: To identify cell surface molecules, which can regulate cell proliferation through cell interaction, monoclonal antibodies (MoAbs) against BMSCs were produced. Among them, 1H8 MoAb, which recognized distinctly an 80 kDa protein, abolished myeloma cell proliferation that was induced by co-culturing with BMSCs. Results: IL-6 gene expression was increased when myeloma or stromal cells were treated with 1H8 MoAb. In addition, the expression of IL-6 receptor and CD40 was up-regulated by 1H8 treatment, suggesting that the molecule recognized by 1H8 MoAb is involved in cell proliferation by modulating the expression of cell growth-related genes. Myeloma cells contain high levels of reactive oxygen species (ROS), which are related to gene expression and tumorigenesis. Treatment with 1H8 decreased the intracellular ROS level and increased PAG antioxidant gene concomitantly. Finally, 1H8 induced the tyrosine phosphorylation of several proteins in U266. Conclusion: Taken together, 1H8 MoAb recognized the cell surface molecule and triggered the intracellular signals, which led to modulate gene expression and cell proliferation.

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A Study on Prediction of Surface Temperature and Reduction of Infrared Emission from a Naval Ship by Considering Emissivity of Funnel in the Mid-Latitude Meterological Conditions (중위도 기상조건에서 함정의 연돌 방사율을 고려한 적외선 복사량 예측 및 감소방안 연구)

  • Gil, Tae-Jun;Choi, Jun-Hyuk;Cho, Yong-Jin;Kim, Tae-Kuk
    • Journal of the Society of Naval Architects of Korea
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    • v.44 no.1 s.151
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    • pp.40-47
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    • 2007
  • This study is focused on developing a software that predicts the temperature distribution and infrared Emission from 30 objects considering the solar radiation through the atmosphere. The solar radiation through the atmosphere is modeled by using the well-known LOWTRAN7 code. Surface temperature information is essential for generating the infrared scene of the object. Predictions of the transient surface temperature and the infrared emission from a naval ship by using the software developed here show fairly good results by representing the typical temperature and emitted radiance distributions expected for the naval ship considered in mid latitude. Emissivity of each material is appeared to be an important parameter for recognizing the target in Infrared band region. The numerical results also show that the low emissivity surface on the heat source can be helpful in reducing the IR image contrast as compared to the background sea.

Effects of Atmospheric Stability and Surface Temperature on Microscale Local Airflow in a Hydrological Suburban Area (대기 안정도와 지표면 온도가 미세규모 국지 흐름에 미치는 영향: 수문지역을 대상으로)

  • Park, Soo-Jin;Kim, Do-Yong;Kim, Jae-Jin
    • Atmosphere
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    • v.23 no.1
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    • pp.13-21
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    • 2013
  • In this study, the effects of atmospheric stability and surface temperature on the microscale local airflow are investigated in a hydrological suburban area using a computational fluid dynamics (CFD) model. The model domain includes the river and industrial complex for analyzing the effect of water system and topography on local airflow. The surface boundary condition is constructed using a geographic information system (GIS) data in order to more accurately build topography and buildings. In the control experiment, it is shown that the topography and buildings mainly determine the microscale airflow (wind speed and wind direction). The sensitivity experiments of atmospheric stability (neutral, stable, and unstable conditions) represent the slight changes in wind speed with the increase in vertical temperature gradient. The differential heating of ground and water surfaces influences on the local meteorological factors such as air temperature, heat flow, and airflow. These results consequentially suggest that the meteorological impact assessment is accompanied by the changes of background land and atmospheric conditions. It is also demonstrated that the numerical experiments with very high spatial resolution can be useful for understanding microscale local meteorology.

Meteorologically Adjusted Ozone Trends in the Seoul and Susan Metropolitan Areas (서울과 부산지역 기상의 영향을 제거한 오존농도 추세)

  • 김유근;오인보;황미경
    • Journal of Korean Society for Atmospheric Environment
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    • v.19 no.5
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    • pp.561-568
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    • 2003
  • Surface ozone concentrations are highly sensitive to meteorological variability. Therefore, in order to reveal the long-term changes in ozone due to the changes in precursor emissions, we need to remove the effects of meteorological fluctuations on the annual distribution of surface ozone. In this paper, the meteorologically adjusted trends of daily maximum surface ozone concentrations in two major Korean cities (Seoul and Busan) are investigated based on ozone data from 11 (Seoul) and 6 (Busan) sites over the period 1992 ∼ 2000. The original time series consisting of the logarithm of daily maximum ozone concentrations are splitted into long-term, seasonal and short-term component using Kolmogorov-Zurbenko (KZ) filter. Meteorological effects are removed from filtered ozone series using multiple linear regression based on meteorologcial variables. The long-term evolution of ozone forming capability due to changes in precursor emission can be obtained applying the KZ filter to the residuals of the regression. The results indicated that meteorologically adjusted long-term daily maximum ozone concentrations had a significant upward trend (Seoul: + 3.02% yr$^{-1}$ , Busan: + 3.45% yr$^{-1}$ ). These changes of meteorologically adjusted ozone concentrations represent the effects of changing background ozone concentrations as well as the more localized changes in emissions.

Immobilized Small Sized Manganese Dioxide Sand in the Remediation of Arsenic Contaminated Water

  • Tiwari, Diwakar;Laldawngliana, C.;Lee, Seung-Mok
    • Environmental Engineering Research
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    • v.19 no.1
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    • pp.107-113
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    • 2014
  • Small sized manganese dioxide particles are immobilized onto the surface of sand by the wet impregnation process. The surface morphology of the solid, i.e., immobilized manganese dioxide natural sand (IMNS) is performed by taking scanning electron microscope images and characterized by the X-ray diffraction data. The specific surface area of the solid is obtained, which shows a significant increase in the specific surface area obtained by the immobilization of manganese dioxide. The $pH_{PZC}$ (point of zero charge) is found to be 6.28. Further, the IMNS is assessed in the removal of As(III) and As(V) pollutants from aqueous solutions under the batch and column operations. Batch reactor experiments are conducted for various physicochemical parametric studies, viz. the effect of sorptive pH (pH 2.0-10.0), concentration (1.0-25.0 mg/L), and background electrolyte concentrations (0.0001-0.1 mol/L $NaNO_3$). Further, column experiments are conducted to obtain the efficiency of IMNS under dynamic conditions. The breakthrough data obtained by the column experiments are employed in non-linear fitting to the Thomas equation, so as to estimate the loading capacity of the column for As(III) and As(V).

C* Based Life Assessment of 3D Crack at High Temperature (C*에 기초한 3차원 고온균열 수명평가)

  • Han, Tae-Soo;Yoon, Kee-Bong;Lee, Hyung-Il
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.25 no.5
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    • pp.823-833
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    • 2001
  • In recent years, the subject of remaining life assessment has drawn considerable attention in chemical plants, where various structural components typically operate at high temperature an pressure. Thus a life prediction methodology accounting for high temperature creep fracture is increasingly needed for the components. Critical defects in such structures are generally found in the form of semi-elliptical surface crack, and the analysis of which is consequently an important problem in engineering fracture mechanics. On this background, we first develop an auto mesh generation program for detailed 3-D finite element analyses of axial and circumferential semi-elliptical surface cracks in a piping system. A high temperature creep fracture parameter C-integral is obtained from the finite element analyses of generated 3-D models. Post crack growth module is further appended here to calculate the amount of crack growth. Finally the remaining lives of surface cracked pipes for various analytical parameters are assessed using the developed life assessment program.

Surface Phenomena of Deuterized Ethanol Exposed Zircaloy-4 Surfaces

  • Park, Ju-Yun;Jung, Se-Won;Chun, Mi-Sun;Kang, Yong-Cheol
    • Bulletin of the Korean Chemical Society
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    • v.30 no.6
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    • pp.1349-1352
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    • 2009
  • We report the results of the surface chemistry of deuterized ethanol exposed Zircaloy-4 (Zry-4) surfaces with various amount of $C_2D_5$OD exposures at 190 K. This system was examined with Auger electron spectroscopy (AES) and temperature programmed desorption (TPD) techniques. In TPD study, $D_2$ was evolved at two different desorption temperature regions accompanying with broad desorption background. The lower temperature feature at around 520 K showed first-order desorption kinetics. The high temperature desorption peak at around 650 K shifted to lower desorption temperature as the exposure of $C_2D_5$OD increased. The Zr(MNV) Auger peak shifted about 2.5 eV from 147 eV to lower electron energy followed by 300 L of $C_2D_5$OD dosing. This implies metallic zirconium was oxidized by deuterized ethanol adsorption. After stepwise annealing of the oxidized Zry-4 sample up to 843 K, the shifted Zr(MNV) peak was gradually shifted back to metallic zirconium peak position. After the sample was heated to 843 K, the oxygen content near the Zry-4 surface was recovered to clean surface level. The concentration of carbon, however, was not recovered by annealing the sample.

Optimization of ultrasound-assisted extraction of glycyrrhizic acid from licorice using response surface methodology

  • Jang, Seol;Lee, A. Yeong;Lee, A. Reum;Choi, Goya;Kim, Ho Kyoung
    • Integrative Medicine Research
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    • v.6 no.4
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    • pp.388-394
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    • 2017
  • Background: The present study optimized ultrasound-assisted extraction conditions to maximize extraction yields of glycyrrhizic acid from licorice. Methods: The optimal extraction temperature ($X_1$), extraction time ($X_2$), and methanol concentration ($X_3$) were identified using response surface methodology (RSM). A central composite design (CCD) was used for experimental design and analysis of the results to obtain the optimal processing parameters. Results: Statistical analyses revealed that three variables and the quadratic of $X_1$, $X_2$, and $X_3$ had significant effects on the yields and were followed by significant interaction effects between the variables of $X_2$ and $X_3$ (p<0.01). A 3D response surface plot and contour plots derived from the mathematical models were applied to determine the optimal conditions. The optimum ultrasound-assisted extraction conditions were as follows: extraction temperature, $69^{\circ}C$; extraction time, 34?min; and methanol concentration, 57%. Under these conditions, the experimental yield of glycyrrhizic acid was 3.414%, which agreed closely with the predicted value (3.406%). Conclusion: The experimental values agreed with those predicted by RSM models, thus indicating the suitability of the model employed and the success of RSM in optimizing the extraction conditions.