• Asian-Australasian Journal of Animal Sciences
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• 제14권3호
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• pp.302-306
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• 2001

This study was carried out to construct mapping population and to evaluate the methods involved, including polymorphic DNA marker system and appropriate statistical analysis. As an initial step to establish chicken genome mapping project, White Leghorn (WL) and Korean Ogol chicken (KOC) were used for generating backcross population. From 8 initial parents, total 280 backcross progenies were obtained and 40 were used for genotyping and linkage analysis. For development of novel polymorphic markers for KOC, Random Amplified Polymorphic DNA (RAPD) markers specific for this chicken line were generated. Also included in this study were six microsatellite markers from East Lansing map as reference loci. For segregation analysis, 15 RAPD markers and 6 microsatellites were used to genotype the backcross population. Among the RAPD markers that we developed, 2 pairs of markers were identified to be linked and another 4 RAPD markers showed linkage with microsatellites of known map. In summary, this study showed that our backcross population generated from the mating of KOC to WL serves as a valuable genetic resource for genotyping. Furthermore, RAPD markers are proved to be valuable in linkage mapping analysis.

• 한국양식학회지
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• 제10권3호
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• pp.363-371
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• 1997

미꾸리 암컷과 미꾸라지 (Misgurnus mizolepis))$\times$미꾸리(M. anguillicaudatus) 잡종 수컷을 교배시켜 역교배체를 생산하였다. 역교배체의 수정율과 부화율은 친어 수컷으로 사용된 미꾸리와 유사하였다. 더욱이 역교배체는 난황흡수기에 저온에 저항성을 보여 전 실험군이 폐사한 미꾸리군에 비하여 단 14%의 낮은 초기 사망률을 나타내어 뚜렷한 생존율 증가가 관찰되었다. 역교배체의 적혈구 크기와 DNA 함량은 친어 암, 수의 중간을 나타내었고 역교배체는 2n=48 혹은 2n=49인 2가지 종류의 2배체 염색체수를 나타내었다. 부화후부터 6개월 동안 동일 조건에서 성장률을 조사한 결과, 역교배체는 친어 암, 수의 중간 성장을 보여 어류양식시 새로운 양식대상 어종으로의 가능성을 시사하였다. 역교배체는 거의 미꾸리와 체색 및 형태에서 유사하였으나 형질계측결과 친어 암수의 중간을 나타내는 형질 및 몇 개의 새로운 획득형질이 관찰되었다. 역교배체는 부화후 2개월 및 부화후 4개월에서 각기 성비를 조직학적으로 조사한 결과 암컷을 전혀 발견할 수 없어 전 수컷 단성집단 생산의 가능성을 보였다.

• 한국양식학회지
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• 제22권1호
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• pp.74-78
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• 2009

동자개(PF) 암컷과 대농갱이(LU) 수컷에 의해 유도된 잡종(FU)을 대상으로 동자개 및 대농갱이 암컷과 역교배체(PFU, LFU)를 유도하여 수정률, 부화율, 초기생존을, 염색체 수 및 핵형분석을 수행하였다. 유도된 PFU ($PF{\times}FU$)의 수정률은 86.8%, 부화율은 70.7% 및 생존율은 76.4%로 양친으로 사용된 PF 및 LU보다 유의적으로 높게 나타났다. 그러나 FU를 사용한 LFU ($PF{\times}FU$)는 모두 사망하였다. PF (2n=52)와 FU(2n=52)간의 PFU의 염색체 수는 2n = 52로 양친과 동일하게 나타났으며 핵형분석 결과 22 metacentrics + 20 submetacentric + 10 acrocentric으로 구성되어 있었다.

• 한국육종학회지
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• 제41권4호
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• pp.463-467
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• 2009

Allium 속 근연종인 파와 양파간 종간교잡을 이용하여 양파로부터 새로운 형질을 도입하는 과정에서 유래되는 종간교잡 F1과 여교잡 세대들의 게놈크기의 변화를 flow cytometry를 이용하여 측정한 결과 다음과 같은 결과를 얻었다. 1. 양파와 파의 종간교잡 F1의 식물학적 특성은 양친의 중간형을 보였으나 여교잡이 진전됨에 따라 반복친인 파의 표현형이 우세하였다. flow cytometry를 이용하여 2C nuclear DNA content을 측정한 결과 $F_1$은 양친에 비해 오히려 증가하였으나 여교잡이 진행됨에 따라 급속히 감소하여 $BC_2F_1$에서는 반복친과 비슷한 수준으로 감소하였다. 여교잡세대의 자식인 $BC_1F_2$은 반복친과 비슷한 $23.1{\pm}1.0pg$으로 측정되었으나 $BC_2F_2$는 $22.9{\pm}0.4pg$으로 반복친의 2C nuclear DNA content인 $23.2{\pm}0.2pg$ 보다 오히려 감소하였다.

• 한국연초학회지
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• 제9권2호
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• pp.11-24
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• 1987

Lines were developed from the two crosses, BY4 X NC 82 and BY 4 X BY 104, by bulk, backcross and anther-derived dihaploid methods of breeding , and their variations in major agronomic, chemical characters, heritability, and response to selection were compared. 1. The anther-derived dihaploid lines showed a greater genetic variability and heritability in characters investigated than the lines developed by the other two methods of breeding . However, those were Inferior In agronomic characters and yielded 15.4% less leaf tobacco than the lines developed by the bulk method. 2. The lines developed by bulk method were higher In the frequency of lines resistant to bacterial wilt and in the yield, but Its lines appeared later flowering. inferior leaf Quality, and higher total nitrogen contents than those developed by the backcross method of breeding. 3. In the population of the lines developed by the backcross method, bacterial wilt resistant lines were less frequent, but the lines with a good leaf quality were more frequent.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.21-27
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• 2003

Manifestation of heterotic genetic interaction was studied in different hybrids made between multivoltine recurrent backcross (RBL)/congenic lines (Con. L) during unfavourable season when temperature and relative humidity are > $30^{\circ}C$ and 86%, respectively. A few number of silkworm race or strain or breed like Nistari (N ＋ p or Np) can sustain the temperature above 3$0^{\circ}C$ and RH above 86%. The present heterosis study screened a hybrid i.e., CB$_{5}$Lm5RBL1M$_{6}$DPC-LmE$^1$RBL and its reciprocal provided heterobeltiotic effect on survival by number and pupation rate at a magnitude of 20% (p < 0.01) and yield by weight of 10% (p < 0.01). Beside all the hybrids expressed heterosis over check - Nistari (N ＋ p) with better quality silk. Therefore, aforesaid hybrid may be useful for utilization at commercial level during adverse seasons of West Bengal.gal.

• Mycobiology
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• 제32권1호
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• pp.6-10
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• 2004

White and brown strains of Flammulina velutipes were inter-crossed. All $F_1$ showed light-brown fruitbody, suggesting that a gene for the brown fruitbody was incompletely dominant against the white one. And backcross experiment showed that more than two genes were involved in color determination. To isolate a molecular marker linked to fruitbody color, a set of primers was designed from a sequence of clones derived by a bulked segregant analysis. These markers showed a specific band which co-segregated with brown fruitbody forming strains.

• International Journal of Industrial Entomology and Biomaterials
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• 제7권1호
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• pp.11-14
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• 2003

NF733xin, the near allele line was obtained by means of crossing and backcrossing the silkworm race T6, which contained fluoride resistance major gene, to race 733xin, which was highly susceptible to fluoride toxicity. Two hundred RAPD random primers were used in the RAPD analysis of these 3 strains. Two molecular markers, OPB-08850 and OPB-10917, were obtained. OPB-10917 was used to detect the backcross generations. It was found that all the fluoride resistant individuals in each backcross generation had the same special band. These results proved that this marker was reliable.

• 한국연초학회지
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• 제16권2호
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• pp.102-107
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• 1994

This study was conducted to transfer the potato virus Y-necrosis strain resistance from Nicotiana africana to Nicotiana tabacum (cv. NC82) N. africana was crossed with NC82, GErmination of the cotyledon stage, at which time most of the seedling died. However, surviving seedlings continued to grow normally. Chromosomes of the these interspecific self-sterile F1 hybrids were doubled by tissue culture. Amphidiploid of F1 hybrid was self-fertile. Starting with amphidipliod, a wystematic backcross (BC) program was set up with NC82 as recurrent parent. In the BC5C2 generation, the resistant plant was selected. This resistant line, KF8833-1, had 48 chromosome and secreting glandular trichomes. It flowered 2days later than NC82, and stalk height, leaves per plant, leaf length and leaf width were similar to those of NC82.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2021년도 춘계학술대회
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• pp.111-111
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• 2021