• Journal of the Korean Magnetic Resonance Society
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• v.12 no.2
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• pp.65-73
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• 2008

HP1298 (Swiss-Prot ID ; P65108) is an 72-residue protein from Helicobacter pylori strain 26695. The function of HP1298 was identified as Translation initiation factor IF-l based on sequence homology, and HP1298 is included in IF-l family. Here, we report the sequence-specific backbone resonance assignments of HP1298. About 97% of all the $^{1}HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$, and $^{13}CO$ resonances could be assigned unambiguously. We could predict the secondary structure of HP1298, by analyzing the deviation of the $^{13}C{\alpha}$ and $^{13}C{\beta}$ shemical shifts from their respective random coil values. Secondary structure prediction shows that HP1298 consists of six $\beta$-strands. This study is a prerequisite for determining the solution structure of HP1298 and investigating the structure-function relationship of HP1298. Assigned chemical shift can be used for the study on interaction between HP1298 and other Helicobacter pylori proteins.

• Journal of KIISE
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• v.41 no.12
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• pp.1145-1151
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• 2014

In this paper, we propose MPLO(Multi-Path routing with Local Optimization) for load balancing in the tactical backbone network. The MPLO manages global metric and local metric separately. The global metric is propagated to other routers via a routing protocol and is used for configuring loop-free multi-path. The local metric reflecting link utilization is used to find an alternate path when congestion occurs. We verified MPLO could effectively distribute user traffic among available routers by simulation.

• Journal of the Korean Magnetic Resonance Society
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• v.24 no.3
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• pp.70-76
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• 2020

The CRISPR-Cas system provides an adaptive immunity for bacteria and archaea against invading phages or foreign plasmids. In the type II CRISPR-Cas system, a single effector protein Cas9 and a guide RNA form an RNA-guided endonuclease complex that can degrade DNA targets of foreign origin. To avoid the Cas9-mediated destruction, phages evolved anti-CRISPR (Acr) proteins that neutralize the host bacterial immunity by inactivating the CRISPR-Cas system. Here we report the backbone ¹H, ¹⁵N, and ¹³C resonance assignments of AcrIIA5 that inhibits the endonuclease activity of type II-A Streptococcus thermophilus Cas9 and also Streptococcus pyogenesis Cas9 using triple resonance nuclear magnetic resonance spectroscopy. The backbone chemical shifts of AcrIIA5 predict a disordered region at the N-terminus, followed by an αββββαβββ fold.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.5C
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• pp.422-434
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• 2002

We propose 3-layered hierarchical fiber-optic backbone transmission network composed of B-DCS, Backbone ring, Edge ring for efficient transmission of high capacity traffic and consider design method to ensure network survivability of each layer at affordable cost. Mathematical ring-construction cost minimization using MIP(Mixed Integer Programming) models results in NP-complete problem. So, it is hard to solve it within reasonable computing time. on a large-scale network. Therefore we develop heuristic algorithms solving WSCAP(Working and Spared Channel Assignment Problem) for B-DCS, MRLB(Multi-Ring Load Balancing) problem for Backbone ring, and ORLB(Overlayed Ring Load Balancing) problem for Edge ring and show their usefulness through case study.

• Journal of the Korean Magnetic Resonance Society
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• v.19 no.3
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• pp.112-118
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• 2015

Hsp31 protein is one of the members of DJ-1 superfamily proteins and has a dimeric structure of which molecular weight (MW) is 62 kDa. The mutation of DJ-1 is closely related to early onset of Parkinson's disease. Hsp31 displays $Zn^{+2}$-binding activity and was first reported to be a holding chaperone in E. coli. Its additional glyoxalase III active has recently been characterized. Moreover, an incubation at $60^{\circ}C$ induces Hsp31 protein to form a high MW oligomer (HMW) in vitro, which accomplishes an elevated holding chaperone activity. The NMR technique is elegant method to probe any local or global structural change of a protein in responses to environmental stresses (heat, pH, and metal). Although the presence of the backbone chemical shifts (bbCSs) is a prerequisite for detailed NMR analyses of the structural changes, general HSQC-based triple resonance experiments could not be used for 62 kDa Hsp31 protein. Here, we prepared the per-deuterated Hsp31 and performed the TROSY-based triple resonance experiments for the bbCSs assignment. Here, detailed processes of per-deuteration and the NMR experiments are described for other similar NMR approaches.

• Bulletin of the Korean Chemical Society
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• v.29 no.2
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• pp.347-351
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• 2008

Acetyl-CoA carboxylase (ACC) is an excellent candidate for antibiotics drug target, which mediates malonyl-CoA synthesis from acetyl-CoA through acetylation process. It is also involved in the committed step of fatty acid synthesis which is essential for living organisms. We have determined the three dimensional structure of C terminal domain of HP0371, biotin-carboxyl carrier protein of H. pyroli, in solution state using heteronuclear multi-dimensional NMR spectroscopy. The structure of HP0371 shows a flatten b-sheet fold which is similar with that of E. coli. However, the sequence and structure of protruding thumb are different with that of E. coli and the thumb shows different basis of structural rigidity based on backbone dynamics data.

• Macromolecular Research
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• v.15 no.2
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• pp.173-177
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• 2007

Living anionic and cationic polymerizations have been combined to prepare various types of comb-like copolymers composed of polystyrene (PS) and polyisoprene (PI) blocks, with a precisely controlled architecture. According to the relative placement of these elementary building blocks, combs with randomly distributed PS and PI or with poly(styrene-b-isoprene) diblock branches (I & II, respectively) can be prepared. The reaction procedure initially includes the synthesis of a poly(chloroethylvinyl ether) using living cationic polymerization, which is used as the reactive backbone to successively graft $PS^-Li^+$ and $PI^-Li^+$ or $PI-b-PS^-Li^+$ to obtain structures (I) or (II). The synthesis of Janus-type PS-comb-b-PI-combs (III) initially involves the synthesis of a diblock backbone using living cationic polymerization, which bears two distinct reactive functions having either a protected or activated form. Living $PS^-Li^+$ and $PI^-Li^+$ are then grafted, in two separate steps, onto each of the reactive functions of the backbone, respectively.

• Molecules and Cells
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• v.20 no.3
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• pp.442-445
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• 2005

HP0894 (SwissProt/TrEMBL ID O25554) is an 88-residue conserved hypothetical protein from Helicobacter pylori strain 26695 with a calculated pI of 8.5 and a molecular weight of 10.38 kDa. Proteins with sequence similarity to HP0894 exist in Vibrio choierae, Enterococcus faecalis, Campylobacter jejuni, Streptococcus pneumoniae, Haemophilus influenzae, Escherichia coli O157, etc. Here we report the sequence-specific backbone resonance assignments of HP0894. About 97.5% (418/429) of the HN, N, CO, $C{\alpha}$, $C{\beta}$ resonances of the 88 residues of HP0894 were assigned. On the basis of these assignments, three helical regions and four strand regions were identified using the CSI program. This study is a prerequisite for calculating the solution structure of HP0894, and studying its interaction with its substrates, if any, and/or with other proteins.

• Korean journal of food and cookery science
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• v.7 no.3
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• pp.29-36
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• 1991

Analysis of major nutritional components and Sensory evaluation in two kinds of beef bone stocks (White & Brown) have been Carried out in this study, these stocks were prepared with four different parts of beef bone (Knee bone, Rumpbone, Legbone, Backbone). White bone stocks were made of each beef bone boiling in water & hours, while brown bone stocks were prepared with roasted beef bone in the oven at $230^{\circ}C$ for half an hour and boiled 8 hours with water. Fatty acids were determined by GLC (Gas Lipids Chromatogram), the minerals were analysed by Automic spectrometer. The results of these analysis were obtained as followes; 1. Neutral lipids was gradually becreased, and glycolipids phospholipids were increased in quantity in Brown stocks for 8 hours. Unsaturated fatty accid of Brown stocks was highly decreased due to roasting of bores in the oven at 23$0^{\circ}C$ for half an hour. But they appeared in large quantity in white stocks. 2. The minerals also contained of high percentage in almost Brown stock except backbone Stock 3. Four materials (Kneebone, Rumpbone, Legbone, and backbone) were used for this study and the paired comparison of flavor test presented the recognition of different flavor at 5% level of Least Significant Difference (LSD) on brown stocks (Kneebone, and Legbone). Ranking preference test showed that white Kneebone stock and brown legbone stock had good taste.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.09a
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• pp.877-883
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• 2009

Shaking table tests are performed to evaluate the dynamic group pile effect in fine sand. Single pile tests and $2{\times}4$ group pile tests are performed on 3D pile spacing. Dynamic p-y backbone curves are obtained to evaluate dynamic group pile effect by using dynamic p-y curve of single pile. And dynamic group pile p-multiplier is estimated by dynamic p-y backbone curve. Dynamic p-multiplier can be calculated by using subground reaction ratio of dynamic p-y backbone curve which is the same displacement of p-y curve peak point As the result, dynamic group pile effects are evaluated in terms of a shaking frequency, a shaking acceleration, and a relative density. Dynamic group pile p-multiplier is the largest at lead pile, and the value decrease at middle pile and trail pile. p multiplier increases as increasing input acceleration and decreasing relative density. This results coincide with NCHRP's research which suggest p multiplier increases as increasing pile cap displacement.