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Biological activity of shizukanols isolated from Chloranthus japonicus roots (홀아비꽃대(Chloranthus japonicus) 뿌리로부터 분리한 shizukanol들의 생물활성)

  • Park, Mi-Ran;Kim, Hae-Young;Choi, Gyung-Ja;Lee, Seon-Woo;Jang, Kyoung-Soo;Kim, Jin-Seog;Hong, Kyung-Sik;Park, No-Joong;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Korean Journal of Pesticide Science
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    • v.8 no.4
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    • pp.338-346
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    • 2004
  • The methanol extract of Chloranthus japonicus roots effectively controlled the development of rice blast(Magnaporthe grisea), rice sheath blight(Corticium sasaki), tomato pay mold(Btrytis cinerea), tomato late blight(Phytophthora infestans), and wheat leaf rust(Puccinia recondita). From the methanol extract of C. japonicus roots, three antifungal substances were isolated. Their chemical structures were determined to be shizukanols B, C, and D mainly by mass and NMR spectral data. Among the three substances, shizukanol C showed the strongest inhibitory activity against mycelial growth of the plant pathogenic fungi tested; it completely inhibited mycelial growth of M. grisea. Colletotrichum gloeosporioides, and C. acutatum at concentrations of more than $12.5{\mu}g$/ and P. infestans at concentrations of more than $3.13{\mu}g/m\ell$. They also controlled effectively the development of rice blast and wheat leaf rust. On the other hand, they caused phytotoxic symptoms on barley leaves and inhibited the growth of duckweed (Lemna paucicostata) with $EC_{50}$ values of $30.0{\mu}g/m\ell$ for shizukanol B, $49.9{\mu}g/m\ell$ for shizukanol C, and $154{\mu}g/m\ell$ for shizukanol D. In addition, shizukanol C showed an insecticidal activity against brown planthopper (Nilaparavata lugens), peen peach aphid (Myzus persicae), diamond-back moth (Plutella xylostella), and tobacco cutworm (Spodoptera litura) of the 5 arthropod pests tested with mortality values of more than 60% at a concentration of $1,000{\mu}g/m\ell$.

Production and Assessing Usefulness of the Moving Phantom for Respiration Gated Radiotherapy (호흡동조 방사선치료용 팬텀의 제작 및 유용성 평가)

  • Lee, Yang-Hoon;Lee, Jae-Hee;Yoo, Suk-Hyun
    • The Journal of Korean Society for Radiation Therapy
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    • v.22 no.2
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    • pp.135-144
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    • 2010
  • Purpose: The purpose of this study is that through production of phantom for respiration gated radiotherapy, assessing appropriacy of exposure dose for the therapy using RPM (Real-time Position Management). Materials and Methods: We located measurement object on the phantom for respiration gated radiotherapy made of 2 linear actuator, acrylic panel, stanchion, iron plate ets. to drive (up, down, front, back). Using 4D CT scan, we analyzed patient's respiration and reproduced the movement by computer. On the phantom, we located a 2D-Array (PTW) and an White water phantom (4.5 cm) and used DMLC (interval 2 cm) in the field size $10{\times}10\;cm$, then exposed 21EX X-ray 100 MU, in the case of phantom was (1) static (2) moving (3) gated using RPM respectively gantry $0^{\circ}$ and $90^{\circ}$ We measured with a 0.125 CC ionization chamber (PTW) on the phantom (7.5 cm) in the same condition. Results: Ionization chamber: There were within 0.3% of error with gating respiration and approximately 2% of error without gating in the same condition. 2D-Array: Gantry $90^{\circ}$, field size $10{\times}10\;cm$, using DMLC. There were within 3% of error with gating respiration and approximately 16% of error without gating. Conclusion: The phantom for respiration gated radiotherapy makes plans considering patient's movement, quantitative analysis of exposure dose and proper assessment therapy for IMRT patients using RPM possible.

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Weed Flora of Cultivated Land and Its Surrounding Area in Cheju Island (제주도(濟州道) 농경지(農耕地) 및 그 주변(周邊)에 발생(發生)하는 잡초종(雜草種))

  • Kim, K.U.;Kim, J.H.;Back, K.W.;Lee, I.J.;Kim, S.H.;Choi, Y.S.
    • Korean Journal of Weed Science
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    • v.9 no.3
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    • pp.201-213
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    • 1989
  • About 233 weed species belonging to 54 families mainly occurring in summer were observed in cultivated land and its surrounding area in Cheju Island. The compositae was the most widely occurring family covering 41 weed species, followed by 28 species in gramineae, 14 in labiatae, 13 in leguminasae and 12 in polygonaceae etc.. In terms of the lands classified about 126 species in 39 families were observed in upland ared and 38 species in 20 families occurred in lowland area, 108 species in 36 families in orchard and 214 species in 50 families observed in non-cultivated area. The most dominant weed species in Cheju Island were Portulaca oleracea, Digitaria sanguinalis in both the upland and orchard, Monochoria vaginalis, Cyperus amuricus in lowland, and Digitaria sanguinalis, Artemisia princeps, Partulaca oleracea in non-cultivated land area. There were two communities in upland area, such as Phyllanthus urinaria, Siegesbeckia orinexitalis etc., and Cephalonaplos segetum, Mosla dianthera etc.. The lowland area composed of one major community like Onenanthe javanica, and Eleocharis acicularis. The orchard consisted of the communities of Stellaria aquatics, Viola mandshurica etc., and Justicia procumbens, Varedellia crustacea etc.. The non-cultivated land composed of two major communities like Amplicarpaed edgeworthii, Lactuca indica etc., and Clematis appiiforia, Aster hispidus etc..

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Effects of Physically Effective Neutral Detergent Fiber Content of TMR Feed on the Productivity and Carcass Characteristics in Hanwoo (Bos taurus coreanae) Steers (TMR 사료의 물리적 유효 NDF 함량이 한우 거세비육우의 생산성과 도체특성에 미치는 영향)

  • Jeon, Byong Tae;Park, Jae Hyun;Cho, Won Mo;Kim, Sang Woo;Jang, Se Young;Moon, Sang Ho
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.35 no.1
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    • pp.12-16
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    • 2015
  • This study was carried out to evaluate accurately physical characteristics of total mixed rations (TMR) by investigating the effects of physically effective neutral detergent fiber content on the growth performance and carcass characteristics in fattening steers. Twelve Hanwoo (Bos Taurus coreanae) steers aged 26 months were used in this trial. Steers were offered one of two total mixed ration (TMR) diets - High or Low physically effective neutral detergent fiber (peNDF) content groups-that were differentiated by mixing time. The carcass traits of the experimental animals were evaluated by Korea Institute for Animal Products Quality Evaluation (KAPE) after slaughter. Body weight gain during the experimental period was significantly higher (P<0.05) in the High group ($30.00{\pm}10.27kg$) compared with the Low group ($17.20{\pm}6.34kg$), and daily weight gain was also high in the former. Although there was no significant difference, the carcass weight was higher in the High group ($414.60{\pm}34.28kg$) compared with the Low group ($377.80{\pm}11.97kg$). However, the beef quality grade of the Low group ($1^{++}$) was higher than that of the High group ($1^+$). Increases in body weight gain, daily gain, and carcass weight for the High group were mainly influenced by a high level of dry matter (DM) intake and digestibility; consequently, back-fat thickness increased and the beef quality grade was lowered. Because growth performance and carcass characteristics were affected by the physical traits of the diet, we suggested that proper peNDF content is also needed for fattening steers.

Quality Comparison of Emulsion- Type Sausages Made from Rhus verniciflua Stokes Fed Pork and Extract (옻 급여 돈육과 추출물로 제조한 유화형 소시지의 품질 비교)

  • Lee Sung Ki;Kang Sun Moon;Kim Yong Sun;Kang Chang Gie
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.210-217
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    • 2005
  • This study was designed to evaluate the quality comparison of emulsion-type sausages made from different Rhus verniciflua Stokes (RVS) sources. The pigs were fed a supplemented concentrate diet with a RVS supplement of $4\%$ feed for 5 weeks before slaughter. The RVS extract was prepared from 100g of RVS sawdust and 1L of distilled water for 48 hours. Emulsion-type sausages were made using lean meat or dietary RVS han1 lean $(51.07\%)$, ice water or RVS extract $(19.63\%)$, back fat $(26.60\%)$ and other additives $(2.70\%)$. The treated sausages were divided into non-dietary meat with water (T1, Control), dietary RVS meat with water (T2), non-dietary meat with RVS extract (T3), and dietary meat with RVS extract (T4). The crude fat was significantly lower (p<0.05) in dietary RVS meat-added sausages (T2, T4) than in control sausage (T1). The lightness ($L^{\ast}$) and redness ($a^{\ast}$) were significantly lower (p<0.05) in RVS extract-added sausages (T3, T4) during refrigerated storage. The $a^{\ast}$ value was higher (p<0.05) in T2 than in the other treatments. The VBN and TBARS values were significantly lower in RVS extract-added sausages (T3, T4) during refrigerated storage. The hardness, adhesiveness, gumminess, and chewiness were significantly lower (p<0.05) in T2 than in the other treatments. This results showed that feeding of RVS in diet and/or RVS extract had a significant impact on the quality of emulsion-type sausage. The RVS extract-added emulsion-type sausages (T3, T4) showed dark and reddish color although they were more effective in delaying the protein deterioration and lipid oxidation. Consequently, the sausage prepared from pigs fed $4\%$ RVS with water (T2) was more effective in increasing the $a^{\ast}$ value, textural properties, and delaying the protein deterioration, lipid oxidation than that without RVS in diet.

Effects of Conjugated Linoleic Acid-Triglyceride Additives on Quality Characteristics of Pressed Ham (Conjugated Linoleic Acid-Triglyceride 첨가가 프레스햄의 품질특성에 미치는 영향)

  • Lee, Jeong-Ill;Ha, Young-Joo;Lee, Jae-Ryung;Joo, Young-Kuk;Kwack, Suk-Joon;Do, Chang-Hee
    • Food Science of Animal Resources
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    • v.27 no.1
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    • pp.8-15
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    • 2007
  • Conjugated linoleic acid (CLA) was chemically synthesized using the alkaline isomerization method of com oil. CLA-TG was synthesized by reaction with sodium methoxide. Five different treatments were devised based on differences in the amount of CLA-TG added into the pressed han. for controls, 10% of pork back fat among the total component was only added without any CLA-TG. For the first treatment, 5% of CLA-TG among the lard component added into the press ham was replaced. For the 2nd, 3rd and 4th treatments, 10%, 15% and 20% of CLA-TG was respectively replaced. Pressed ham manufactured using CLA-TG was vacuum packaged and then stored for 1, 7, 14, 21 and 28 days at $4^{\circ}C$. Samples were analyzed for chemical composition, physico-chemical properties (pH, color), and texture characteristics. Typical chemical composition characteristics (moisture, crude protein, crude fat) were not significantly different between controls and CLA-TG treatment groups. Crude fat content of CLA-TG treatment groups was significantly lower than that of controls (p<0.05). pH values of controls was higher than that of CLA-TG treatment groups. The pH of control and CLA-TG treatment groups increased significantly as the storage period increased (p<0.05). Meat color (CIE $L^*,\;b^*$) of CLA-TG treatment groups was higher than that of controls. $a^*$ values were decreased by replacement of CLA-TG, but appeared to be unaffected by storage length. There was no significant difference in texture between controls and CLA-TG treatment groups. Based on these findings, we conclude that the physico-chemical properties and texture characteristics of manufactured pressed ham were not affected by CLA-TG addition. Also, our results indicate that high-quality pressed ham can be manufactured with CLA accumulation.

Residue Study for Bisphenol A in Agricultural Reservoirs (농업용 저수지에서 Bisphenol A의 잔류실태조사)

  • Cho, Il Kyu;Nam, Hyo Song;Jeon, Yongbae;Park, Jun sung;Na, Tae Wong;Kim, Back Jong;Kan, Eunsung
    • Korean Journal of Environmental Agriculture
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    • v.35 no.4
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    • pp.270-277
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    • 2016
  • BACKGROUND: Significant amount of bisphenol A has been released from the manufacturing process of plastics, epoxy resins, dental material and flame retardants. Bisphenol A has been detected at trace levels in wastewater, surface water, landfill leachate and drinking water. However, the residual survey of bisphenol A has not been performed in agricultural reservoir beside agricultural environment cultivating crops. This study was conducted to monitor the residual bisphenol A in national agricultural reservoirs and understand a level of contamination of bisphenol A in the agricultural environment in Korea. METHODS AND RESULTS: The water and water sediment were collected at agricultural reservoirs in Chungnam, Chungbuk, Kyunggi, Jeonnam, Jeonbuk, Kyungnam and Kyungbuk province. Bisphenol A was analyzed by the LC-MS/MS with triple quad 4500. The recovery of water and water sediment in the agricultural reservoirs showed the level of 95.7~97.2% and 91.5~100.3%, respectively. CONCLUSION: Bisphenol A was detected at the level of $0.05{\mu}g/L{\sim}0.18{\mu}g/L$ and $0.1{\mu}g/kg{\sim}34{\mu}g/kg$ in water and water sediment of the reservoirs, respectively. Based on the results, the residue of bisphenol A will be tried in the crops surrounding these agricultural reservoirs where bisphenol A detected above $10{\mu}g/kg$ of bisphenol A.

Effects of Feeding Type of Concentrates During Growing Period and Slaughter Age on Growth Performance, Feed Efficiency and Carcass Characteristics in Growing-Fattening Holstein Steers (홀스타인 거세우에 대한 육성기 농후사료 급여형태 및 출하월령이 성장발육, 사료이용성 및 도체특성에 미치는 효과)

  • Kang, S.W.;Jeong, H.Y.;Ahn, B.S.;Oh, Y.K.;Son, Y.S.
    • Journal of Animal Science and Technology
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    • v.46 no.6
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    • pp.989-998
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    • 2004
  • The objectives of this work was to investigate the effects of feeding type of concentrates during growing period and slaughter age on growth performance, feed efficiency and carcass characteristics in growing-fattening Holstein steers. Treatments were two concentrates feeding types(ad libitum or restricted 1.9% of BW) and three slaughter ages(at 19, 22 or 25 mo). Body weight at slaughter was numerically higher(approx. 8.3%) for the restricted than the ad libitum feeding groups. TDN intake rate was higher(8.4%) for the restricted than the ad libitum feeding groups. One mo slaughter delay increased TDN intake by 4%. Despite the lack of significances, higher back fat thickness and rib-eye area appeared in the restricted feeding or the late slaughter groups compared with the ad libitum feeding or the early slaughter groups. Marbling score(mean 2.46) numerically increased when the steers were restrictively fed concentrates compared with the ad libitum feeding groups. In addition, significant increases in the marbling score were associated with the slaughter age delay. In economic analysis, increases in total income were associated with the restricted feeding and the slaughter age delay. Whereas the highest monthly income appeared in the restricted feeding-and-slaughter at 22 mo group. In conclusion, the optimal feeding system may be 1) the restricted concentrates feeding(1.9% of BW) during growing period and 2) the late slaughter age(22${\sim}$25 mo of age) in growing-fattening Holstein steers fed rice straw.

Effects of Rubus Coreanus Miq. Oil on Serum Lipids in C57BL/6J Mice (복분자씨유의 식용유지 대체가 C57BL/6J Mice의 혈청 지질에 미치는 영향)

  • Byun, Moon-Sun;Cha, Youn-Soo;Hwang, Keum-Taek;Yu, Ok-Kyeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.7
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    • pp.953-960
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    • 2015
  • This study evaluated the effects of Rubus coreanus Miq. oil on the plasma lipid profile of high fat diet (HFD)-induced obese mice. Animals were randomly divided into 4 groups (n=10). After completion of the 5-week experimental period, we measured bodyweight gain, food intake, adipose tissue mass, and plasma lipid profile. We also analyzed the activities of carnitine and superoxide dismutase (SOD) involved in ${\beta}$-oxidation and antioxidation, respectively. Our results show that HFD-induced weight gain in animals in the R. coreanus Miq. oil diet group (RCO) and corn oil diet group (CO) was significantly lower compared to animals in the HFD group; RCO supplementation had a more noticeable effect than CO. Visceral and back fat weights were lower in the RCO and CO groups while plasma HDL cholesterol (HDL-C) and HDL-C per total cholesterol [HDL-C/TC (%)] ratio were significantly higher in the RCO group. The contents of acid-soluble acylcarnitine and total carnitine as well as SOD activation were significantly higher in the RCO group, but no significant difference was observed between the RCO and CO groups. In conclusion, RCO effectively averted elevation of total body weight and fat weight in HFD-induced obese mice and promoted increased HDL-C. Therefore, R. coreanus Miq. oil might play an anti-obesity role in obese people and could be used as an effective oil supplement.

Clinical research of Aqua-acupuncture effects on Rheumatoid arthritis (약침을 이용한 류마토이드 관절염에 대한 임상적 연구)

  • Park, So-Young;Koh, Kang-Hoon;Yoon, Min-Young;Jin, Kyong-Sun;Chang, Byoung-Sun;Km, Il-Du;Cho, Nam-Geun;Lee, Byong-Chul;Lee, Sam-Ro;Moon, Hyung-Cheol;Hwang, Woo-Jun;Lee, Geon-Mok
    • Journal of Acupuncture Research
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    • v.19 no.1
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    • pp.135-146
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    • 2002
  • Background and Purpose : This study shows the clinical effects on treatment with Rheumatoid arthritis using Aqua-acupuncture such as Ursi Fel Bovis Calculus and Cathami Flos that are known for which have anti-inflammatory and analgesic effects. Objective and Methods : This study has been carried out Aqua-acupuncture treatment such as Cathami Flos and Ursi Fel Bovis Calculus twice or three times a week with patients diagnosed as rheumatoid arthritis on 7 criteria of US Rheumatism Academy. Results : 1. If you see the sexual distribution, it shows the rates of male and female 1: 1.83. Age distribution shows as age ascend it shows high. 2. If you see the duration distribution, it shows chronic tendency in order of more than 6 months(63.3%), 2 to 5 months, and I month. 3. If you see the invaded distribution, it shows chronic tendency in order of hand(37 cases), wrist joint(34 cases), knee joint(32 cases), ankle joint(21 cases), elbow joint(16 cases), shoulder joint(14 cases), feet(14 cases), hip joint(9 cases), low back(6 cases), neck(4 cases). 4. Seeing the mean post-therapeutic index among groups on RA factor level distinction, it shows that 0-18(normal range) scored 89.0, 19-50 scored 94.8, 51-80 scored 101.2, 81-100 scored 97.3, 101-140 scored 102.4, above 141 scored 97.5. 5. Seeing the mean post-therapeutic index among groups on CRP level distinction, it shows that 0-0.4(normal range) scored 100, 0.5-1.0 scored 48.6, 1.1-2.0 scored 34.9, 2.1-3.0 scored 35.7, 3.1-4.0 scored 37.1, 4.1-5.0 scored 53.8, above 5.0 scored 70.4. 6. Seeing the mean post-therapeutic index among groups on ESR level distinction, it shows that 0-20(normal range) scored 48.6, 21-40 scored 57.5, 41-60 scored 59.2, 61-80 scored 59.2, above 81 scored 66.0. 7. If you see the satisfactory assesment after Aqua-acupuncture treatment such as Cathami Flos and Ursi Fel Bovis Calculus, Excellent is 11 cases, Good is 14 cases, and Moderate 5 cases. Conclusion : It will be better effects, if herbal medicine, moxibustion, and acupuncture are used together with Cathami Flos and Ursi Fel Bovis Calculus on rheumatoid arthritis as chronic inflammatory disease.

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