• Journal of the Korean Applied Science and Technology
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• v.37 no.6
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• pp.1535-1544
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• 2020

The primary objective of this study was to isolate and identify amine oxidase-producing probiotic Bacillus strains from traditional fermented soybean paste. Biogenic amines (BA)-forming bacteria isolated from the samples were identified as Bacillus sp. TS09, Bacillus licheniformis TS17, Bacillus subtilis TS19, Bacillus cereus TS23, Bacillus sp. TS30, Bacillus megaterium TS31, B. subtilis TS44, Bacillus coagulans TS46 and Bacillus amyloliquefaciens TS59. Meanwhile, B. subtilis TS04 and TS50 isolated from the same samples exhibited good probiotic properties, including the tolerance to artificial gastric juice and bile salts, the adhesion to intestinal epithelial cells, and the production of bacteriocin(s) active against BA-forming bacteria (Bacillus sp. TS30 and B. subtilis TS44). In addition, the amine oxidase produced by B. subtilis TS04 and TS50 significantly decreased the formation of BA, especially cadaverine, putrescine, and tyramine, therefore, these strains could be considered good potential probiotic candidates to prevent or reduce BA accumulation in food products.

• The Korean Journal of Mycology
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• v.40 no.4
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• pp.277-281
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• 2012

Bacillus sp. NAAS-1 isolated from the field of Chili pepper was tested for biocontrol activity against anthracnose pathogen of Chili pepper caused by Colletotrichum acutatum. The antifungal activity of Bacillus sp. NAAS-1 culture broth was compared with synthetic fungicide containing carbendazim (40%) and kasugamycin (3.45%). Bacillus sp. NAAS-1 showed a similar fungicidal activity against the anthracnose pathogen at the concentration of 50 ${\mu}L/mL$ in comparison to the fungicide containing carbendazim (40%) and kasugamycin (3.45%) using a cup method. Bacillus sp. NAAS-1 also exhibited its potent fungicidal activity against the anthracnose in vivo test at the concentration of 50 ${\mu}L/mL$ when compared to the fungicide containing carbendazim (40%) and kasugamycin (3.45%).

• Microbiology and Biotechnology Letters
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• v.19 no.6
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• pp.543-547
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• 1991

An alkalophilic microorganism, SH-8, was isolated from soil samples. It was a Grampositive, catalase-positive, spore-forming and motile rod which was capable of growth in aerobic condition at the initial pH 9.0 or above up to 11.0 and between 15 and $42^{\circ}C$ The characteristics of this strain resembled those of the Bacillus group of bacteria. The mutant, Bacillus sp. SH- 8M, was selected from Bacillus sp. SH-8 by U.V. mutagenesis and was able to grow at pH 6.9 up to 11.0. These two strains will be suitable for the comparative study on growth and enzyme production at various pH conditions.

• Journal of Life Science
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• v.21 no.10
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• pp.1481-1486
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• 2011

Spent mushroom substrate (SMS) is a by-product remained after a crop of mushrooms. About seven thermophilic strains were isolated from SMS (Flammulina velvtipes). Among them, one isolate, designated UJ03, showed the antifungal activity against Aspergillus flavus and Aspergillus ochraceous producing mycotoxin on PDA medium, potentially. The strain UJ03 produced cellulase and xylanase as extracellular hydrolases. The strain UJ03 was identified as a member of the genus Bacillus by biochemical characteristics using Bacillus ID kit and VITEK 2 system. Comparative 16S rDNA gene sequence analysis showed that strain UJ03 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus amyloliquefaciens with sequence similarity of 98.9%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, strain UJ03 was classified within the genus Bacillus, for which the name Bacillus sp. UJ03 is proposed. The antifungal compound from Bacillus sp. UJ03 was similar to lipopeptide iturin A of Bacillus sp.

• Journal of the Korea Safety Management & Science
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• v.19 no.2
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• pp.95-100
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• 2017

In this study, the bacterial contamination level of equipments and devices in the nuclear medicine department of a university hospital was investigated. CNS was detected from the sample collected from the door opening button of the nuclear medicine department. Bacillus sp. was detected from the table and CNS with Bacillus sp. were detected from the control button at the PET-CT room no.1. Also, CNS was detected from the table and the control button at the PET-CT room no.2. In the distribution room no.1, CNS and Bacillus sp. were detected while CNS being detected from the distribution room no.2 and CNS with Bacillus sp. being detected from the distribution room no.3. In the injection room, Enterrococcus faecium and Pontoea sp. were detected. On the table of the ecsomatics room, Pontoea sp. was detected. Bacillus sp. was detected from the inside of the syringe Pb shield and CNS was detected from the outside. Enterrococcus faecium was detected from the Gamma camera table and Bacillus sp. was detected from the door grip. On the chair at the patient waiting room, Pseudomonas aeruginosa abd Bacillus sp. were detected. Therefore, it was understood that infection should be prevented by securely sterilizing examination devices after each examination, maintaining cleanliness by regular sterilization of waiting chairs and such objects with a number of direct contacts with patients, and infection education for the features of nuclear department.

• Korean Journal of Organic Agriculture
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• v.14 no.4
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• pp.399-410
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• 2006

This study was carried out to identity the effects of antifungal bacteria isolated from the soil grown kiwi fruit plants on the growth inhibition of Botrytis cinerea causing gray mold in kiwi fruit plants in the southern districts of Jeonnam. Two hundred and fifty antagonistic microorganisms were isolated and examined into the antifungal activity against Botrytis cinerea. We screened and isolated four bacterial strains which strongly inhibited Botrytis cinerea from the soil grown kiwi fruit plants. And the best antifungal bacterial strain which called CHO 163 was finally selected. Antagonistic microorganism CHO 163 was identified to be the genus Bacillus sp. based on the morphological and biochemical characterization. Bacillus sp. CHO 163 showed 86.9% of antifungal activity against Botrytis cinerea. By the bacterialization of culture broth and heated filtrates of culture broth, Bacillus sp. CHO 163 showed almost all of antagonistic activity against Botrytis cinerea. And we also confirmed that in vitro the treatment of Bacillus sp. CHO 163 cultured by SD+B+P broth efficiently controled the growth of Botrytis cinerea causing gray mold in kiwi fruit plants.

• Korean Journal of Environmental Agriculture
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• v.20 no.1
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• pp.63-66
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• 2001

To investgate growth effect of tomato by Bacillus sp. WRD-1 isolated from soil, the Bacillus sp. WRD-1 cultures were treated into tomato cultivated soil with different dilutions (1:100, 1:300, and 1:500) and autoclaved Bacillus cultures as control. Growth and yeild of tomato enhanced in treatments of the Bacillus cultures compared to control. The populations of native bacteria and actinomyces were increased twice in field treated with Bacillus sp. WRD-1 cultures, but the number of mold was decreased. Since the Bacillus sp. WRD-1 promoted growth of tomato and affected population dynamics of microorganism in field, this strain is prominent candidate as a microbial biocide to improve soil potential.

• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.603-612
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• 2004

This study was carried out to investigate the replacing fish meal or soybean meal effect of with feather meal or Bacillus sp. inoculated feather meal in broiler diets on the performances and nutrient utilization. One hundred and eighty broilers were randomly allotted to six dietary treatments(1) control, basal diet; (2) BFMl00, Fish meal replacing at 100% level with bacillus sp. inoculated feather meal; (3) BSM20, Soybean meal replacing at 20% level with bacillus sp. inoculated feather meal; (4) BFMl00+BSM20, Fish meal and soybean meal replacing at 100% level and 20% level with bacillus sp. inoculated feather meal, respectively; (5) GFMl00, Fish meal replacing at 100% level with general feather meal; and (6) GSM20, Soybean meal replacing at 20% level with general feather meal) in a 5 week feeding trial. In overall period, body weight gain of control was the highest(1,623g) and those of BFM 100, BFM 100+ BSM 20 and GFM 100 were 1,572g, 1,564g, and 1,078g, respectively. And that of GFM 100(1,078.3g) was the lowest(p<0.05) among treatments. Digestibility of dry matter for BFM 100+BSM 20(81.46%) was higher(p<0.05) than that for other treatments, and that of crude protein was the lowest (p<0.05). Digestibilities of organic matter were significantly(p<0.05) improved when Bacillus sp. inoculated feather meal was replaced at 100% level with fish meal in the basal diet. Methane and hydrogen sulfide gases from the feces were significantly(p<0.05) decreased in chicks fed the control, BFM 100 and GFM 100 diets, when observed after 3 weeks of feeding trials. Feed costs of the control and BFM 100 were 417 and 384 won, respectively but that of BSM 20 was 558 won. Therefore, replacement of fish meal with Bacillus sp. inoculated feather meal in the diets for chicks could be useful for economic production.

• Journal of Korean Society on Water Environment
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• v.21 no.1
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• pp.73-78
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• 2005

This study was performed to examine the disinfection capability of a Rotating Activated Bacillus Contactor (RABC) system, in which the predominant species, Bacillus sp. was expected to have a removal or inactivation effect of total coliforms. In a settling test with mixtures of E. coli and Bacillus sp., a high removal of E. coli was observed at $20{\sim}40^{\circ}C$, while insignificant removal at $10^{\circ}C$. In a batch test, a 4.5% addition of Bacillus sp. to activated sludge considerably enhanced the removal effect of total coliforms, indicating Bacillus sp. played an important role in improving the settlability of the sludge and coliforms. In a pilot scale RABC system, the concentration of total colifroms reduced remarkably in the settling tank, suggesting that total coliforms in the RABC process were eliminated through coagulation and precipitation, probably due to extracellular polymeric substance (EPS) of Bacillus sp. The fraction of Bacillus sp. in the total cell count in the RABC process was in the range of 4.5%~6.3%. The majority (75%) of the Bacillus sp. in the RABC process was Bacillus subtilis which is known to enhance coagulation and precipitation by producing EPS. Hence, an adoption of a RABC process might be able to eliminate the disinfection unit process from a wastewater treatment system.

• Journal of Life Science
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• v.12 no.6
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• pp.745-751
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• 2002

Several bacterial strains producing biosurfactants were isolated from polluted marine and soil by oil. One of the strains named LSC11 showed strong production activity of biosurfactants. This strain was identified as a Bacillus sp. LSC11 based on the morphological, biochemical, and physiological characteristics. The biosurfactant, produced by the strain, emulsified crude oil, vegetable oil, and hydrocarbons. The surface tension of the culture broth of Bacillus sp. LSC11 decreased to 32 mN/m. The crude biosurfactant was obtained from the culture broth by acid precipitation, freeze drying, solvent extraction, and evaporation. The emulsifying activity of the biosurfactant showed better than the chemically synthesized surfactant (SDS, Span40, Span 85). The biosurfactants had strong properties as an emulsifying agent and as an emulsion-stabilizing agent.