• Title/Summary/Keyword: average yield rate

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Isolation of Biopolymer-producing Bacterium and Its Growth Pattern (Biopolymer 생산세균의 분리 및 증식패턴)

  • Yoo, Jin-Young;Chung, Dong-Hyo
    • Microbiology and Biotechnology Letters
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    • v.17 no.5
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    • pp.421-428
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    • 1989
  • A soil bacterium synthesizing an extremely viscous biopolymer was isolated and identified as Pseudomonas delafieldii. The optimal pH and temperature for the growth were 6.5 and 3$0^{\circ}C$, respectively. Maximum specific growth rate was 0.24 h$^{-1}$. The specific polysaccharide productivity, growth yield and product yield were 6.25 mg/g-cell/h, 54.5% and 38.39%, respectively. The polysaccharide was presumed to be $\beta$-glucan containing glucose and gluconolactone (1.9:1.0 in molar ratio) and 1.35 % acetyl group, Element analysis showed that it contained carbon (31.85%) and hydrogen (5.15%). The weight average molecular weight by GPC was 5.64$\times$10$^7$. The intrinsic viscosity was 42.84 dl/g.

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Enzymatic Production of D-Tagatose, a Sugar-substituting Sweetener, from D-Galactose

  • Noh, Hoe-Jin;Kim, Pil
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2000.04a
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    • pp.68-75
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    • 2000
  • D-Tagatose is a potential bulking agent in food as a non-calorific sweetener. To produce D-tagatose from cheaper resources, plasmids harboring the L-arabinose isomerase gene (araA) from Escherichia coli was constructed because L-arabinose isomerase was previously suggested as an enzyme that mediates the bioconversion of galactose to tagatose as well as that of arabinose to ribulose. In the cultures of recombinant E.coli with pTC101, which harboring araA of E.coli, tagatose was produced from galactose in 9.9 % yield. The enzyme extract of E.coli containing pTC101 also converted galactose into tagatose in 96.4 % yield. For the economic production of D-tagatose, an L-arabinose isomerase of E.coli was immobilized using covalent binding on agarose. While the free L-arabinose isomerase produced tagatose with the rate of 0.48 mg/U$.$day, the immobilized one stably converted galactose into average 7.5 g/l$.$day of tagatose during 7 days with higher productivity of 0.87 mg/U$.$day. In the scaled up immobilized enzyme system, 99.9 g/l of tagatose was produced from galactose with 20 % equilibrium in 48 hrs. The process was stably repeated additional 2 times with tagatose production of 104.1 and 103.5 g/l.

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Preparation of High Molecular Weight Poly(methyl methacrylate) with High Yield by Room Temperature Suspension Polymerization of Methyl Methacrylate

  • Lyoo, Won-Seok;Noh, Seok-Kyun;Yeum, Jeong-Hyun;Kang, Gu-Chan;Ghim, Han-Do;Lee, Jinwon;Ji, Byung-Chul
    • Fibers and Polymers
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    • v.5 no.1
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    • pp.75-81
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    • 2004
  • To obtain high molecular weight (HMW) poly(methyl methacrylate) (PMMA) with high conversion, methyl methacrylate (MMA) was polymerized in suspension using a room temperature initiator, 2,2'-azobis(2,4-dimethylvaleronitrile) (ADMVN), and the effects of polymerization conditions on the polymerization behavior of MMA and the molecular parameters of PMMA were investigated. On the whole, the experimental results well corresponded to the theoretically predicted tendencies. These effects could be explained by a kinetic order of ADMVN concentration calculated by an initial rate method and an activation energy difference of polymerization obtained from the Arrhenius plot. Suspension polymerization at 25℃ by adopting ADMVN proved to be successful in obtaining PMMA of HMW (number-average degree of polymerization (P/sub n/): 30,900-36,100) and of high yield (ultimate conversion of MMA into PMMA: 83-93 %) with diminishing heat generated during polymerization. The P/sub n/ and lightness were higher and polydispersity index was lower with PMMA polymerized at lower temperatures.

Development of Pilot-Scale Scrubber for Simultaneous Removal of $SO_2/NO$

  • Jung, Seung-Ho;Jeong, Gwi-Taek;Lee, Gwang-Yeon;Park, Don-Hee;Cha, Jin-Myeong
    • 한국생물공학회:학술대회논문집
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    • 2005.10a
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    • pp.468-474
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    • 2005
  • SOx and NOx are known major precursors of acid rain and thus the abatement of their emissions is a major target in air pollution control. To obtain basic data on the removal process of simultaneous $SO_2/NO$, the optimal reaction condition and the composition of reaction solution for simultaneous removal of $SO_2/NO$, ware investigated using a bubble column reactor. Pilot scrubber was consisted of scrubber, filter and control box. Dust removal rate was 83, 92, and 97% with catalyst flux of 0.5, 0.8, 1.5 L/min, respectively Average dust removal efficiency with a kind of nozzle was about 94 and 90% in STS FF6.5 (5/8in.) and 14 of P.P W(1.0in.), respectively Dust and $SO_2$ were removed more than 98-96% regardless of reactor number. In the case of NO gas, removal yield of 83.3% was achieved after 48 hours in 1 stage, also removal yield of 95.7% was reached in 2 stages. In tile case of application of STS (5/8 in.) and P.P (1.0 in.) as used fill packing, removal efficiency was reached higher than 98% without related to of kind of fill packing.

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Breeding of a New Bright Yellow Gerbera 'Sunmyo' with High Yield for Cut Flower (황색 절화용 다수성 거베라 신품종 '선묘' 육성)

  • Park, Jae-Suk;Chung, Mi-Young;Kim, Chang-Kil
    • Horticultural Science & Technology
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    • v.29 no.2
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    • pp.156-159
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    • 2011
  • A new bright yellow gerbera (Gerbera hybrida Hort.), 'Sunmyo' was bred from a cross between red medium-gerbera 'Beauty' and yellow gerbera 'Tamara' in 2001. Characteristics trials were conducted three times from 2004 to 2006. Bred cultivar 'Sunmyo' showed bright yellow color (YG12B), black center, semi-double type, and middle size flower with stable flower shape. The average yield of 'Sunmyo' increased up to 37% (70.0 stems per year) as compared to control cultivar 'Biro' (51.0 stems per year). The vase life is 7.5 days. The survival rate is 85.0%, and days to first flowering are 95 in the plastic house passing the winter season.

Characteristics of F$_2$Hybrids from Crosses between Korean Cultivars and Canadian Cultivars in Buckwheat(Fagopyrum esculentum Moench.)

  • Yoon, Kyoung-Min;Hong, Soon-Kwan;Park, Cheol-Ho
    • Plant Resources
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    • v.5 no.1
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    • pp.45-50
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    • 2002
  • Korean cultivars of buckwheat(Fagopyrum esculentum Moench.) was crossed with Canadian cultivars in order to improve seed yield as well as leaf production of buckwheat for using as food and medicine. The agronomic characteristics and rutin contents of F$_2$ hybrids are investigated for further selection of superior lines. Dry weight per plant was the highest in a line 1110(6.71g) and leaf weight per plant was the highest in a line 1110(1.91g). Hybrid seeds were 0.55 - 0.70cm long and 0.37 - 0.47cm wide on average. 100 seeds weight ranged from 2.57g to 3.58g. Line 1076 produced the longest seeds(0.70cm) and line 1186 was the longest in seed width. Line 1196 showed the highest 100 seeds weight(3.58g). The highest frequency of the LWR(length/width rate) was 0.66~0.70, indicating that seed shape of the hybrids was mostly oval. Line 1087 showed the highest contents of rutin(77.26ppm). Lines 1090 and 1181 contained respectively rutin of 54.76ppm and 54.35ppm in the seeds. From the yield and rutin point of view, the most superior lines was line 1087 among the lines used for this study.

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Preparation of High Molecular Weight Atactic Poly(vinyl alcohol) by Photo-induced Bulk Polymerization of Vinyl Acetate

  • Lyoo, Won-Seok;Ha, Wan-Shik
    • Fibers and Polymers
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    • v.2 no.2
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    • pp.108-115
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    • 2001
  • Vinyl acetate was polymerized in ultraviolet-ray initiated bulk system at low temperatures using 2,2-azobis(2,4-dimethylvaleronitrile) (ADMVN) or 2,2-azobis(isobutyronitrile) (AIBN) as the photoinitiator, respectively. High molecular weight (HMW) poly(vinyl alcohol) (PVA) having number-average degree of polymerization ($P_n$) of 3,900-7,800 and syndiotactic diad (S-diad) content of 52.5-54.0% could be prepared by complete saponification of synthesized linear poly(vinyl acetate) (PVAc) having $P_n$ 5,900-9,400 obtained at conversion of below 30%. $P_n$ of PVA using ADMVN was larger than that of PVA using AIBN. On the other hand, conversion of the former was smaller than that of the latter, and it was found that the initiation rate of the ADMVN was lower than that of AIBN. This could be explained by a fact that the rate of photolysis of AIBN is faster than that of ADMVN due to the higher quantum yield or dissociation rate constant of AIBN than that of ADMVN. The $P_n$, syndiotacticity, and whiteness of PVA from PVAc polymerized at lower temperatures were superior to those of PVA from PVAc polymerized at higher temperatures.

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Dynamic Effects for Crushing Strength of Rectangular Tubular Members (사각 튜브 부재의 압괴강도에 대한 동적 영향 평가)

  • P.D.C.,Yang
    • Bulletin of the Society of Naval Architects of Korea
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    • v.27 no.1
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    • pp.17-23
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    • 1990
  • When a thin walled member is subjected to compression in a condition such as collision, the energy is mainly absorbed by axial crumpling. In this case, dynamic crushing strength of the member is increased due to the effects of strain-rate compared with the static strength, even though the inertia effect is neglected. In this paper, the method of predicting the static crushing for tubular members is presented using the kinematic method of plasticity. Since, a predicted crushing load, taking account of the dynamic yield stress, usually overestimates the effects of strain-rate, the average plastic flow stress for the effects of strain-rate is used to obtain the dynamic crushing load for tubular members. The analytical results are compared with the experiments published in references, and a good correlation is observed.

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Modern Sedimentary Environment of Jinhae Bay, SE Korea

  • Park, Soo-Chul;Lee, Kang-Wook
    • Journal of the korean society of oceanography
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    • v.31 no.2
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    • pp.43-54
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    • 1996
  • Jinhae Bay, one of the largest tidal bays on the southern coast of Korea, is an area with thick accumulations of recent, fine-grained sediments, mainly supplied from the Nakdong River. The preponderance of silt and clay particles reflects the large quantity of sediments transported in suspension. Although the clay mineral assemblage is similar to that derived from the nearby Nakdong River, relatively high concentration (3-9%) of smectite suggests some local input of fine particles from several streams around the bay or some contribution from the offshore water that may be influenced by the Tsushima Current. The content of organic matters in sediments is as high as 12%, and their C/N ratios imply that they are comprised of mixtures derived from marine plankton and terrestrial plants. $^{210}Pb$ excess activity profiles of sediment cores yield an average sedimentation rate (a 100-year time scale) of about 2-5 mm/yr, which coincides well with the long-term sedimentation rate (a 1000-year time scale) estimated from the sediment isopach map. On the basis of sediment bulk density and sedimentation rate, an annual sink of mud in the bay is estimated approximately 1.0 ${\times}$ $10^{6}$ tons per year.

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Occurrence of plant parasitic nematodes in Codonopsis lanceojata field and its damage by Meloidogyne hapia (더덕포장 선충발생 상황 및 당근뿌리혹선충에 의한 피해)

  • Lim, Ju-Rak;Hwang, Chang-Yeon;Hwang, Jong-Yeon;Park, Chun-Bong;Kim, Dae-Hyang;Choi, Jung-Sick;Choo, Byug-Bil
    • Korean journal of applied entomology
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    • v.44 no.4 s.141
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    • pp.317-323
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    • 2005
  • This study was conducted to survey plant-parasitic nematodes and their damages on Codonopsis lanceolata Trautv at 116 fields of 15 major cultivation areas in Korea. Among the 9 plant-parasitic nematode genera detected in the fields, Ditylenchus citri, Meloidogyne hapla, M. incognita, Pratylenchus neglectus, Tylenchorynchus clayton were identified. M. hapla Chitwood showing the highest field infection rate of 61.9% followed by Tylenchus spp. 16.1%, Pratylenchus neglectus 7.8%, Ditylenchus citri 5.1%, and Helicotylenchus sp. 5.1%. Average field infection rate of root-knot nematodes in 2-year-old C. lanceolata was 67.0%, and the average plant infection rate was 60.2%. The average yield decrease rate was 10%. The damage rate by the root-hot nematode increased as the cultivation year extended in Jeonbuk province, and the damage was also higher in flat land than in mountainous areas.