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The Quantity and Pattern of Leaf Fall and Nitrogen Resorption Strategy by Leaf-litter in the Gwangneung Natural Broadleaved Forest (광릉숲 천연활엽수림의 수종별 낙엽 현상과 질소 재전류 특성)

  • Kwon, Boram;Kim, Hyunseok;Yi, Myong Jong
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.3
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    • pp.208-220
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    • 2019
  • The seasonality of leaf fall has important implications for understanding the response of trees' phenology to climate change. In this study, we quantified the leaf fall pattern with a model to estimate the timing and speed of leaf litter according to species and considered the nutrient use strategy of canopy species. In the autumns of 2015 and 2016, leaf litter was collected periodically using 36 litter-traps from the deciduous forests in Gwangneung and sorted by species. The seasonal leaf fall pattern was estimated using the non-linear regression model of Dixon. Additionally, the resorption rate was calculated by analyzing the nitrogen concentration of the leaf litter at each collection time. The leaf litter generally began in early October and ended in mid-November depending on the species. At the peak time (T50) of leaf fall, on average, Carpinus laxiflora was first, and Quercus serrata was last. The rate of leaf fall was fastest (18.6 days) for Sorbus alnifolia in 2016 and slowest (40.8 days) for C. cordata in 2015. The nitrogen resorption rates at T50 were 0.45% for Q. serrata and 0.48% for C. laxiflora, and the resorption rate in 2015 with less precipitation was higher than in 2016. Since falling of leaf litter is affected by environmental factors such as temperature, precipitation, photoperiod, and $CO_2$ during the period attached foliage, the leaf fall pattern and nitrogen resorption differed year by year depending on the species. If we quantify the fall phenomena of deciduous trees and analyze them according to various conditions, we can predict whether the changes in leaf fall timing and speed due to climate change will prolong or shorten the growth period of trees. In addition, it may be possible to consider how this affects their nutrient use strategy.

A Study on the Evaluation and Maintenance for Alternative Habitats of the Narrow-mouth Frog (Kaloula borealis) - A Case Study on the Alternative Habitats of Kaloula borealis at the University of Seoul - (맹꽁이 대체서식지 조성 평가 및 유지관리 방안 연구 - 서울시립대학교 맹꽁이 대체서식지를 사례로 -)

  • Park, Seok-Cheol;Han, Bong-Ho;Park, Min-Jin
    • Journal of the Korean Institute of Landscape Architecture
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    • v.47 no.1
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    • pp.76-87
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    • 2019
  • The purpose of this study was to evaluate the performance of and to derive future maintenance-management measures of the constructed alternative habitat for the Kaloula borealis at the University of Seoul, examining the period between 2015-2017. The research was constructed in 2014 and in a $191m^2$ area. The performance evaluation was divided into maintaining the habitat of the target species, maintaining the population and reproduction rates of the target species, maintaining the habitat of the wild species, the resilience of natural ecosystems, and the harmony with the surrounding environment. In terms of maintaining the habitat of the target species, soil collected from the existing habitat of the Kaloula borealis and was the depth was increased to 30cm in the alternative habitat. An artificial water supply was required every year during the supporting the spawning and hatching of other amphibians along with the Kaloula borealis. The sources of water of the alternative habitat were both rain and tap water, as it cannot be maintained naturally. Additionally, the Kaloula borealis thrived because it inhabited the research site and the average temperature was $26.2^{\circ}C$ from April-June, which is when the Kaloula borealis spawns. In terms of maintaining the population and reproduction rates of the Kaloula borealis, they were evaluated to have stable rates of reproduction. In terms of maintaining the habitat of the wild species, studies on vegetation and the structure of the characteristics of prey or predators will be needed. Also, alien species, such as Humulus japonicus and Bidens frondosa needed to be removed to maintain the wetland ecosystem of the wild species. In the assessment of the resilience of the natural ecosystems, the mud was monitored, noting the changes in the depth of water, with steps taken to reduce the leakage of water. The mud collected from the Haneul Pond wetland, which is located around the research site was piled up. Also, partial mowing management and the inducement of a natural vegetation colony was required for vegetation management. It was also necessary to create porous spaces, such as old trees and tree branches to create a habitat with hiding places and feeding and spawning places for small organisms. In terms of the harmony with the surrounding environment, the following threat factors needed to be managed: amphibian roadkill by vehicles and pedestrians and artificial draining due to nearby user access. Based on the monitoring results, alternative habitat management measures presented the promoting various waterside structures, in which amphibians can spawn and hide in, managing the water environment consistently, managing the vegetation, focused on the habitat of the wild species, and managing the surrounding environment for the habitat. The creation of an alternative habitat should be managed through monitoring, reflecting the characteristics of the changes in the site. Also continuing efforts are also needed to improve the habitat of the target species.

Production of Medium-chain-length Poly (3-hydroxyalkanoates) by Pseudomonas sp. EML8 from Waste Frying Oil (Pseudomonas sp. EML8 균주를 이용한 폐식용류로부터 medium-chain-length poly(3-hydroxyalkanoates) 생합성)

  • Kim, Tae-Gyeong;Kim, Jong-Sik;Chung, Chung-Wook
    • Journal of Life Science
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    • v.31 no.1
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    • pp.90-99
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    • 2021
  • In this study, to reduce the production cost of poly(3-hydroxyalkanoates) (PHA), optimal cell growth and PHA biosynthesis conditions of the isolated strain Pseudomonas sp. EML8 were established using waste frying oil (WFO) as the cheap carbon source. Gas chromatography (GC) and GC mass spectrometry analysis of the medium-chain-length PHA (mcl-PHAWFO) obtained by Pseudomonas sp. EML8 of WFO indicated that it was composed of 7.28 mol% 3-hydrxoyhexanoate, 39.04 mol% 3-hydroxyoctanoate, 37.11 mol% 3-hydroxydecanoate, and 16.58 mol% 3-hydroxvdodecanoate monomers. When Pseudomonas sp. EML8 were culture in flask, the maximum dry cell weight (DCW) and the mcl-PHAWFO yield (g/l) were showed under WFO (20 g/l), (NH4)2SO4 (0.5 g/l), pH 7, and 25℃ culture conditions. Based on this, the highest DCW, mcl-PHAWFO content, and mcl-PHAWFO yield from 3-l-jar fermentation was obtained after 48 hr. Similar results were obtained using 20 g/l of fresh frying oil (FFO) as a control carbon source. In this case, the DCW, the mcl-PHAFFO content, and the mcl-PHAFFO yields were 2.7 g/l, 62 wt%, and 1.6 g/l, respectively. Gel permeation chromatography analysis confirmed the average molecular weight of the mcl-PHAWFO and mcl-PHAFFO to be between 165-175 kDa. Thermogravimetric analysis showed decomposition temperature values of 260℃ and 274.7℃ for mcl-PHAWFO and mcl-PHAFFO, respectively. In conclusion, Pseudomonas sp. EML8 and WFO could be suggested as a new candidate and substrate for the industrial production of PHA.

Effect of Different Cooling System on Performance and Hair Cortisol on Sows under Heat Stress (고온기 분만사 내 냉방 시설의 종류가 모돈의 번식성적 및 스트레스 지표에 미치는 영향)

  • Oh, Seung-Min;Choi, Yo-Han;Kim, Dong-Woo;Ha, Sang-Hun;Kim, Jo-Eun;Jung, Hyun-Jung;Kim, Jin-Soo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.3
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    • pp.160-168
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    • 2021
  • This study was undertaken to determine the effect of different cooling systems on the performance and hair cortisol of sows under heat stress. During a 21-day experiment, a total of 40 multiparous sows (Landrace×Yorkshire; 242.84±2.89 kg) were allotted to 4 treatments, each with 10 replicates (1 sow per pen). The experimental treatments were CP (Cooling pad), AC (Air conditioner), SC (Snout cooling), and MS (Mist spray). We observed an increase in the average daily feed intake during lactation (p<0.05) in the CP and AC treatment groups. AC treatment had the highest (p<0.05) and SC treatment had the lowest (p<0.05) piglet weight at weaning. During lactation, sows administered SC and MS treatments had higher (p<0.05) hair cortisol accumulation, as compared with the AC and CP treatments. Hair cortisol accumulation in piglets during lactation was highest with MS treatment (p<0.05), and lowest after CP treatment (p<0.05). MS treatment had the highest (p<0.05), and AC treatment had the lowest (p<0.05) respiratory rate and rectal temperature during lactation. In conclusion, our results indicate that a cooling pad and air conditioning cooling system increases the productivity of a sow, as compared to snout cooling and mist spray cooling systems.

A case study on monitoring the ambient ammonia concentration in paddy soil using a passive ammonia diffusive sampler (논 토양에서 암모니아 배출 특성 모니터링을 위한 수동식 암모니아 확산형 포집기 이용 사례 연구)

  • Kim, Min-Suk;Park, Minseok;Min, Hyun-Gi;Chae, Eunji;Hyun, Seunghun;Kim, Jeong-Gyu;Koo, Namin
    • Korean Journal of Environmental Biology
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    • v.39 no.1
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    • pp.100-107
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    • 2021
  • Along with an increase in the frequency of high-concentration fine particulate matter in Korea, interest and research on ammonia (NH3) are actively increasing. It is obvious that agriculture has contributed significantly to NH3 emissions. However, studies on the long-term effect of fertilizer use on the ambient NH3 concentration of agricultural land are insufficient. Therefore, in this study, NH3 concentration in the atmosphere of agricultural land was monitored for 11 months using a passive sampler. The average ambient NH3 concentration during the total study period was 2.02 ㎍ m-3 and it was found that the effect of fertilizer application on the ambient NH3 concentration was greatest in the month immediately following fertilizer application (highest ambient NH3 concentration as 11.36㎍ m-3). After that, it was expected that the NH3 volatilization was promoted by increases in summer temperature and the concentration in the atmosphere was expected to increase. However, high NH3 concentrations in the atmosphere were not observed due to strong rainfall that lasted for a long period. After that, the ambient NH3 concentration gradually decreased through autumn and winter. In summary, when studying the contribution of fertilizer to the rate of domestic NH3 emissions, it is necessary to look intensively for at least one month immediately after fertilizer application, and weather information such as precipitation and no-rain days should be considered in the field study.

Production of Poly (3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Bacillus sp. EMK-5020 Using Makgeolli Lees Enzymatic Hydrolysate and Propionic Acid as Carbon Sources (막걸리 주박 가수분해 산물과 propionic acid를 탄소원으로 이용한 Bacillus sp. EML-5020 균주로부터 poly (3-hydroxybutyrate-co-3-hydroxyvalerate) 생합성)

  • Kwon, Kyungjin;Kim, Jong-Sik;Chung, Chung-Wook
    • Journal of Life Science
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    • v.32 no.7
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    • pp.510-522
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    • 2022
  • In this study, to biosynthesize PHA with properties more similar to polypropylene, a Bacillus sp. EMK-5020 strain that biosynthesized poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was isolated from soil. Bacillus sp. EMK-5020 strain biosynthesized PHBV containing 1.3% 3-hydroxyvalerate (3HV) using reducing sugar contained in Makgeolli lees enzymatic hydrolysate (MLEH) as a single carbon source. As the amount of propionic acid, which was added as a second carbon source, increased, the content of 3HV also increased. PHBV containing up to 48.6% of 3HV was synthesized when 1.0 g/l of propionic acid was added. Based on these results, the strain was cultured for 72 hr in a 3 l fermenter using reducing sugar in MLEH (20 g/l) and propionic acid (1 g/l) as the main and secondary carbon sources, respectively. As a result, 6.4 g/l DCW and 50 wt% of PHBV (MLEH-PHBV) containing 8.9% 3HV were biosynthesized. Through gel permeation chromatography and thermogravimetric analysis, it was confirmed that the average molecular weight and the decomposition temperature of MLEH-PHBV were 152 kDa and 273℃, respectively. In conclusion, the Bacillus sp. EMK-5020 strain could biosynthesize PHBV containing various 3HV fractions when MLEH and propionic acid were used as carbon sources, and PHBV-MLEH containing 8.9% 3HV was confirmed to have higher thermal stability than standard PHBV (8% 3HV).

Effect of Pre-harvest Irradiation of UV-A and UV-B LED in Ginsenosides Content of Ginseng Sprouts (새싹 인삼의 수확 전 UV-A 및 -B LED의 조사에 의한 진세노사이드의 영향)

  • Jang, Seong-Nam;Lee, Ga-Oun;Sim, Han-Sol;Bae, Jin-Su;Lee, Ae-Ryeon;Cho, Du-Yong;Cho, Kye-Man;Son, Ki-Ho
    • Journal of Bio-Environment Control
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    • v.31 no.1
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    • pp.28-34
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    • 2022
  • This study was conducted to determine the changes in ginsenosides content according to additional UV-A, and UV-B LED irradiation before harvesting the ginseng sprouts. One-year-old ginseng seedlings (n=100) were transplanted in a tray containing a ginseng medium. The ginseng sprouts were grown for 37 days at a temperature of 20℃ (24h), a humidity of 70%, and an average light intensity of 80 µmol·m-2·s-1 (photoperiod; 24h) in a container-type plant factory. Ginseng sprouts were then transferred to a custom chamber equipped with UV-A (370 nm; 12.90 W·m-2) and UV-B (300 nm; 0.31 W·m-2) LEDs and treated for 3 days. Growth parameters and ginsenoside contents in shoot and root were conducted by harvesting on days 0 (control), 1, 2, and 3 of UV treatments, respectively. The growth parameters showed non-significant differences between the control and the UV treatments (wavelengths or the number of days). Ginsenoside contents of the shoot was highly improved by 186% in UV-A treatment compared to the control in 3 days of the treatment time. The ginsenoside contents of the roots was more improved in UV-A 1-day treatment and UV-B 3-day treatment, compared to the control by 171% and 160%, respectively. As a result of this experiment, it is thought that UV LED irradiation before harvesting can produce sprout ginseng with high ginsenoside contents in a plant factory.

Analysis of Rice Blast Outbreaks in Korea through Text Mining (텍스트 마이닝을 통한 우리나라의 벼 도열병 발생 개황 분석)

  • Song, Sungmin;Chung, Hyunjung;Kim, Kwang-Hyung;Kim, Ki-Tae
    • Research in Plant Disease
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    • v.28 no.3
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    • pp.113-121
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    • 2022
  • Rice blast is a major plant disease that occurs worldwide and significantly reduces rice yields. Rice blast disease occurs periodically in Korea, causing significant socio-economic damage due to the unique status of rice as a major staple crop. A disease outbreak prediction system is required for preventing rice blast disease. Epidemiological investigations of disease outbreaks can aid in decision-making for plant disease management. Currently, plant disease prediction and epidemiological investigations are mainly based on quantitatively measurable, structured data such as crop growth and damage, weather, and other environmental factors. On the other hand, text data related to the occurrence of plant diseases are accumulated along with the structured data. However, epidemiological investigations using these unstructured data have not been conducted. The useful information extracted using unstructured data can be used for more effective plant disease management. This study analyzed news articles related to the rice blast disease through text mining to investigate the years and provinces where rice blast disease occurred most in Korea. Moreover, the average temperature, total precipitation, sunshine hours, and supplied rice varieties in the regions were also analyzed. Through these data, it was estimated that the primary causes of the nationwide outbreak in 2020 and the major outbreak in Jeonbuk region in 2021 were meteorological factors. These results obtained through text mining can be combined with deep learning technology to be used as a tool to investigate the epidemiology of rice blast disease in the future.

Analysis of Hydrodynamics in a Directly-Irradiated Fluidized Bed Solar Receiver Using CPFD Simulation (CPFD를 이용한 태양열 유동층 흡열기의 수력학적 특성 해석)

  • Kim, Suyoung;Won, Geunhye;Lee, Min Ji;Kim, Sung Won
    • Korean Chemical Engineering Research
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    • v.60 no.4
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    • pp.535-543
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    • 2022
  • A CPFD (Computational particle fluid dynamics) model of solar fluidized bed receiver of silicon carbide (SiC: average dp=123 ㎛) particles was established, and the model was verified by comparing the simulation and experimental results to analyze the effect of particle behavior on the performance of the receiver. The relationship between the heat-absorbing performance and the particles behavior in the receiver was analyzed by simulating their behavior near bed surface, which is difficult to access experimentally. The CPFD simulation results showed good agreement with the experimental values on the solids holdup and its standard deviation under experimental condition in bed and freeboard regions. The local solid holdups near the bed surface, where particles primarily absorb solar heat energy and transfer it to the inside of the bed, showed a non-uniform distribution with a relatively low value at the center related with the bubble behavior in the bed. The local solid holdup increased the axial and radial non-uniformity in the freeboard region with the gas velocity, which explains well that the increase in the RSD (Relative standard deviation) of pressure drop across the freeboard region is responsible for the loss of solar energy reflected by the entrained particles in the particle receiver. The simulation results of local gas and particle velocities with gas velocity confirmed that the local particle behavior in the fluidized bed are closely related to the bubble behavior characterized by the properties of the Geldart B particles. The temperature difference of the fluidizing gas passing through the receiver per irradiance (∆T/IDNI) was highly correlated with the RSD of the pressure drop across the bed surface and the freeboard regions. The CPFD simulation results can be used to improve the performance of the particle receiver through local particle behavior analysis.

Changes in Distribution of Debris Slopes and Vegetation Characteristics in Mudeungsan National Park (무등산국립공원의 암설사면 분포변화 및 식생 특성)

  • Seok-Gon Park;Dong-Hyo Kim
    • Korean Journal of Environment and Ecology
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    • v.37 no.1
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    • pp.1-12
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    • 2023
  • We analyzed the distribution area of debris slopes in Mudeungsan (Mt. Mudeung) National Park by comparing aerial photos of the past (1966) and the present (2017) and identified the vegetation characteristics that affect the change in the area of the debris slopes by investigating the vegetation status of the debris slopes and the surrounding areas. The area of debris slopes in Mt. Mudeung appears to have been reduced to a quarter of what it used to be. Debris slopes here have decreased at an average rate of 2.3 ha/yr over 51 years by vegetation covers. Notably, most of the small-area debris slopes in the low-inclination slopes disappeared due to active vegetation coverage. However, there are still west-facing, south-west-facing, south-facing, and large-area debris slopes remaining because the sun's radiant heat rapidly raises the surface temperature of rock blocks and dries moisture, making tree growth unfavorable. Because of these locational characteristics, the small-scale vegetation in the middle of Deoksan Stony Slope, which is the broadest area, showed distinct characteristics from the adjacent forest areas. Sunny places and tree species with excellent drying resistance were observed frequently in Deoksan Stony Slope. However, tree species with high hygropreference that grow well in valleys with good soil conditions also prevailed. In some of these places, the soil layer has been well developed due to the accumulation of fine materials and organic matter between the crevices of the rock blocks, which is likely to have provided favorable conditions for such tree species to settle and grow. At the top of Mt. Mudeung, on the other hand, the forest covered the debris slopes, where Mongolian oaks (Quercus mongolica) and royal azaleas (Rhododendron schlippenbachii), which typically grow in the highlands, prevailed. This area was considered favorable for the development of vegetation for the highlands because the density of rock blocks was lower than in Deoksan Stony Slope, and the soil was exposed. Moreover, ash trees (Fraxinus rhynchophylla) and Korean maple trees (Acer pseudosieboldianum) that commonly appear in the valley areas were dominant here. It is probably due to the increased moisture content in the soil, which resulted from creating a depressive landform with a concave shape that is easy to collect rainwater as rock blocks in some areas fell and piled up in the lower region. In conclusion, the area, density of the rock blocks, and distribution pattern of rock block slopes would have affected the vegetation development and species composition in the debris slope landform.