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Bactericidal Efficacy of Non-thermal DBD Plasma on Staphylococcus aureus and Escherichia coli (비열 유전체장벽방전 플라즈마의 포도상구균 및 대장균 살균효과)

  • Kim, Keyyoung;Paik, Namwon;Kim, Yonghee;Yoo, Kwanho
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.28 no.1
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    • pp.61-79
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    • 2018
  • Objectives: The objective of this study was to examine the effect of non-thermal dielectric barrier discharge(DBD) plasma on decontamination of Staphylococcus aureus(S. aureus) and Escherichia coli(E. coli) as common pathogens. Methods: This experiment was carried out in a chamber($0.64m^3$)designed by the authors. The plasma was continuously generated by a non-thermal DBD plasma generator(Model TB-300, Shinyoung Air tech, Korea). Suspensions of S. aureus and E. coli of 0.5 McFarland standard($1.5{\times}10^8CFU/mL$) were prepared using a Densi-Check photometer(bio $M{\acute{e}}rieux$, France). The suspensions were diluted1:1000 in sterile PBS solutions(approximately$10^{4-5}CFU/mL$) and inoculated on tryptic soy agar(TSA) in Petri dishes. The Petri dishes(80mm internal diameter)were exposed to the non -thermal DBD plasma in the chamber. Results: The results showed that 95% of S. aureus colonies were killed after a six-hour exposure to the DBD plasma. In the case of E. coli, it took two hours to kill 100% of the colonies. The gram-negative E. coli had a greater reduction than the gram-positive S. aureus. This difference may be due to the structure of their cell membranes. The thickness of gram-positive bacteria is greater than that of gram-negative bacteria. The S. aureus is more resistant to DBD plasma exposures than is E. coli. It should be noted that average concentrations of ozone, a byproduct of the DBD plasma generator, were monitored throughout the experiment and the results were well below the criteria, 50 ppb, recommended by the Korean Ministry of the Environment. Thus, non-thermal DBD plasma is deemed safe for use in hospital and public facilities. Conclusions: There was evidence that non-thermal DBD plasma can effectively kill S. aureus and E. coli. The results indicate that DBD plasma technology can greatly contribute to the control of infections in hospitals and other public and private facilities.

A Study on the Emission and Dispersion of Particulate Matter from a Cement Plant (한 시멘트공장의 분진발생과 대기확산에 관한 조사연구)

  • Chang, Man-Ik;Chung, Yong;Kwon, Sook-Pyo
    • Journal of Preventive Medicine and Public Health
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    • v.16 no.1
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    • pp.67-77
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    • 1983
  • To investigate the an air pollution by particulate matter and its dispersion, a cement plant produceing portland cement 600,000 ton/year and its vicinity were surveyed from Obtober, 1980 to April, 1983. The survey was mainly focused on main stack emmission rate of the cement plant and particle size distribution in the dust, dustfall and total suspended particulate concentration in the area by month and distance from the stack. The results of the study were as follows; 1. The main stack emission rate was surveyed before and after the spray tower was additionally installed to the original E.P bag filter. Before the spray tower installed, the main stack emission rate was higher ($0.64g/Nm^3$) than the emission standard of Korean Environmental Preservation Law's ($0.59g/Nm^3$, amended to $0.4g/Nm^3$ on April 1983), but after the spray tower was installed, its main stack emission rate was markedly decreased to the standard ($0.43g/Nm^3$). 2. $2{\sim}3{\mu}m$ of the particle size was the largest portion (20.8%) of the dust particulate from the main stack and 50% of the frequency distribution was $1.5{\mu}m$ of the size. Most particle size was below $10{\mu}m$. 3. The spray tower reduced the dustfall to $37.81{\sim}9.76\;ton/km^2/month$ while dustfall appeared at $45.29-15.45ton/km^2/month$, in the vicinity of plant before spray tower installed 4. Mean concentrations of total suspended particulate for 24 hours of the various stations were determined in $20.6-200.0{\mu}g/m^3$, 3 stations of tham were higher than the value of Harry and William's arthmetic average standard $130{\mu}g/m^3$. 5. Linear regression between dustfall [X] and total suspended particulate[Y] concentration was an equation, Y=4.024X+11.479.[r=0.91] 6. During the whole seasons in the opposite area 100m apart from the omission source the prevailing wind direction was with estimated more than $30ton/km^2/month$, and the concentration of total suspended particulate for 24 hours averaging time was more than $140{\mu}g/m^3$ in the same area and direction. 7. Assuming the wind direction were constant through the day dustfalls for a day were estimated at $13.40ton/km^2/day,\;10.79ton/km^2/day$ and $4.55ton/km^2/day$ at various distances of 100m, 500m and 1,500m from the emission source respectively. 8. In the simutalion of dustfall and suspended dust by area, Gaussian dispersion model modified by size distribution of particulate matter was not applicated since the emission of dust were from multi sources other them stack. From the above results, it could be applied that the dispersion of dust from the cement plant is estimated and regulated for the purpose of environmental protection.

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Groundwater Flow Modeling in a Riverbank Filtration Area, Deasan-Myeon, Changwon City (창원시 대산면 강변여과수 취수부지 주변의 지하수 유동 모델링)

  • Hamm, Se-Yeong;Cheong, Jae-Yeol;Kim, Hyoung-Su;Hahn, Jeong-Sang;Cha, Yong-Hoon
    • Economic and Environmental Geology
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    • v.38 no.1
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    • pp.67-78
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    • 2005
  • Riverbank filtration has been used in advanced countries for 150 years. In Korea, investigations for producing riverbank filtrate started in the Han River, Nakdong River, Geum River, Yeongsan River and Seomjin River basins in the 1990s. The lower part of the Nakdong River has a poorer water quality than the upper part of the river. A water balance analysis and groundwater flow modeling were conducted for the riverbanks of the Nakdong River in Daesan-Myeon, Changwon City. The results of the water balance analysis revealed the groundwater infiltration rate into the aquifer to be 245.26 mm/year (19.68% of the average annual precipitation, 1,251.32 mm). Direct runoff accounts for 153.49 mm/year, evapotranspiration is 723.95 mm/year and baseflow is 127.63 mm/year. According to the groundwater flow modeling, 65% of the total inflow to the pumping wells originates from the Nakdong River, 13% originates from the aquifer in the rectilinear direction, and 22% originates from the aquifer in the parallel direction. The particle tracking model shows that a particle moving from the river toward the pumping wells travels 100 m in 50 days and a particle from the aquifer toward the pumping wells travels 100 m in 100 days.

Factors Affecting Intention to Use Smartphone Healthcare Applications (스마트폰 헬스케어 어플리케이션 수용의도에 영향을 미치는 요인)

  • Park, Mijeong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.4
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    • pp.143-153
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    • 2017
  • This was a descriptive survey to determine the intention of users to use smartphone healthcare applications (SHAs) and to clarify factors that may influence such intention. The data were collected during the month of April in 2015, using a structured self-report questionnaire that was distributed to 300 participants aged 20 to 70 years; 285 complete copies were used for the final analysis. The data were analyzed using descriptive statistics, independent t-test, one-way ANOVA, Pearson correlation coefficients, and hierarchical multiple regression. First, according to the results, the average intention to use SHAs was 3.28 out of 5, which varied according to age, final education, economy level, vacation, current disease, total period of smartphone use, and etc. Second, significant correlations were shown by exercise behavior, dietary management behavior, stress management, satisfaction with smartphone use, and satisfaction with using SHAs. Third, the explanatory power of the predictive model involving all general, health-related, smartphone use-related, and SHA use-related factors was 45.5%; and the economic level, interest, status, and awareness satisfaction of patients using SHA were identified to be the main influential factors. The results indicate that SHA developers need to put efforts into improving consumers' app recognition and to develop plans in provoking consumers' interests to increase the use of SHAs.

Real-time Hand Region Detection based on Cascade using Depth Information (깊이정보를 이용한 케스케이드 방식의 실시간 손 영역 검출)

  • Joo, Sung Il;Weon, Sun Hee;Choi, Hyung Il
    • KIPS Transactions on Software and Data Engineering
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    • v.2 no.10
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    • pp.713-722
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    • 2013
  • This paper proposes a method of using depth information to detect the hand region in real-time based on the cascade method. In order to ensure stable and speedy detection of the hand region even under conditions of lighting changes in the test environment, this study uses only features based on depth information, and proposes a method of detecting the hand region by means of a classifier that uses boosting and cascading methods. First, in order to extract features using only depth information, we calculate the difference between the depth value at the center of the input image and the average of depth value within the segmented block, and to ensure that hand regions of all sizes will be detected, we use the central depth value and the second order linear model to predict the size of the hand region. The cascade method is applied to implement training and recognition by extracting features from the hand region. The classifier proposed in this paper maintains accuracy and enhances speed by composing each stage into a single weak classifier and obtaining the threshold value that satisfies the detection rate while exhibiting the lowest error rate to perform over-fitting training. The trained classifier is used to classify the hand region, and detects the final hand region in the final merger stage. Lastly, to verify performance, we perform quantitative and qualitative comparative analyses with various conventional AdaBoost algorithms to confirm the efficiency of the hand region detection algorithm proposed in this paper.

Biomass and Net Primary Production of Quercus variabilis Natural Forest Ecosystems in Gongju, Pohang, and Yangyang Areas (공주(公州), 포항(浦港), 그리고 양양(襄陽) 지역(地域) 굴참나무 천연림(天然林) 생태계(生態系)의 물질생산(物質生産)에 관(關)한 연구(硏究))

  • Park, Gwan-Soo;Lee, Sung-Woo
    • Journal of Korean Society of Forest Science
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    • v.90 no.6
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    • pp.692-698
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    • 2001
  • This study has been carried out to estimate aboveground biomass and net primary production(NPP) in an average 41-years-old Quercus variabilis stand of Gongju area, 45-years-old Quercus variabilis stand of Pohang area, and 54-years-old Quercus variabilis stand of Yangyang area. Ten sample trees were cut in each forest and soil samples were collected in July to August, 2000. Estimation for aboveground biomass and net primary production were made by the equation model $Wt=aD^b$ where Wt is oven dry weight in kg and D is DBH in cm. Total aboveground biomass was 91.31ton/ha in Gongju area, 207.6ton/ha in Pohang area, and 71.39ton/ha in Yangyang area. The aboveground biomass 207.6ton/ha in Pohang area is the highest biomass production among the amount of biomass in Quercus variabils stands reported in Korea. The proportion of each tree component to total aboveground biomass was high in order of bolewood, bolebark, branches and leaves in the three forests. Aboveground total net primary production was estimated at 7.8ton/ha in Gongju area, 11.5ton/ha in Pohang area, and 6.40ton/ha in Yangyang area. There were at least 2 times higher total aboveground biomass in Pohang area than in the Gongju and Yangyang areas because of climate difference among the study areas.

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Health Educational Program for Women's Health in Women's Health Care Center (여성건강교육 프로그램 개발에 관한 연구 - 여성건강간호센터에서의 교육을 중심으로 -)

  • Lee, Eun-Hee;Choi, Sang-Soon;So, Ae-Young
    • Women's Health Nursing
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    • v.6 no.1
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    • pp.67-81
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    • 2000
  • The purpose of this study was to analyze women's health problems using Green & Kreuter's(1991) PRECEDE model and to develop health education program for women's health. The subjects were recruited women from Wonju city 18 years or older. 1. The results showed that about 50% of the women were satisfied with their lives as women, 23% of the sample felt there was a need for a women's health care center. The mean number of health problems was 3.1 and the prevalence rate, 44.4%. 2. We developed on educational program according to group differences related to health problems, diagnosis of disease, variables influencing health promotion behavior, and programs which each group wanted. Also we stressed self-efficacy and self-help group for the management of individual health to all groups. 3. The diagnoses of diseases that were experienced premarital over the past year were gastritis, bronchitis, spinal disk, and fracture, for the childbearing/rearing group, gastritis, vaginitis and cervicitis, cervical cancer and cystitis and nephritis and arthritis and for the middle-aged/elderly group arthritis, gastritis, vaginitis and cervicitis, and spinal disk. Of the sample 30.5% did not have a health exam in the past year, and only 10% of the premarital group, 12.5% of the childbearing/child-rearing group, and 18.3% of the middle aged-elderly group were concerned about their health and did something for their health. 4. The average score on the HPLP was 2.41. the HPLP was scores according to group were found to have significant differences. self-efficacy, family functions, health attention and were considered important variables in the premarital group, in the childbearing/child-rearing group self-efficacy, family functions, internal locus of control, health attention, and health perception and power others locus of control and then for the middle aged-elderly group self-efficacy, health attention, internal locus of control, family functions and health perception. 5. There are a few educational programs in the city provided by the Wonju Health Center and by community health nurse practitioners. The premarital group requested the educational programs on diet, health exercise, family health and stress management. Also the childbearing/child-rearing group wanted programs on diet, family health, stress management, and health exercise, and the middle-aged/elderly group wanted that of family health, diet, climacteric changes stress management and health exercise. The program suggested that this program should be applied to women in the community to insure adequate management of women's health. Follow-up research with PROCEED is needed to analyze health outcomes, also, a women's health nursing specialist system is required to develop health promotion, and improve the quality of life for women.

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Genetic Parameter Estimates for Productive Traits in Duroc Pigs (듀록종의 산육형질에 대한 유전모수 추정)

  • Cho, Chung-Il;Choy, Yun-Ho;Choi, Jae-Kwan;Choi, Tae-Jeong;Lee, Seung-Su;Cho, Kwang-Hyun;Park, Byoung-Ho
    • Journal of agriculture & life science
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    • v.46 no.5
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    • pp.57-63
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    • 2012
  • The purpose of this study was to estimate genetic parameters for productive traits in Duroc breed. In this study, 40,657 records for productive traits and the pedigree data of 47,974 families were collected from 41 farms registered at the Korean Animal Improvement Association (KAIA) from 2004 to 2011. The REMLf90 program was used to analyze a multiple traits animal model with fixed effects of sex, contemporary group, parity and age at the end of the test as covariate and random effects of animal and residual error. The heritabilities of days to 90 kg (D90KG), average daily gain (ADG), backfat thickness (BF) and eye muscle areas (EMA) were estimated to be 0.334, 0.340, 0.335, and 0.200, respectively. The genetic correlation coefficients were -0.992 between D90KG and ADG, -0.142 between ADG and BF, -0.361 between ADG and EMA, and -0.243 between BF and EMA. Conversely, positive genetic correlations for D90KG with BF and EMA were 0.13 and 0.36, respectively.

Water Resources and Demand in the Namgang Sub-basin (남강 중권역의 수자원 부존량과 용수 수요량의 비교 평가)

  • Choi, Young-Wan;Kim, Yong-Wan;Park, Jeong-Won;Park, Tae-Yang;Jang, Min-Won
    • Journal of agriculture & life science
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    • v.44 no.6
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    • pp.171-182
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    • 2010
  • Water demand and resource at a watershed scale were investigated to prepare for long-term water planning of the Namgang sub-basin. The quantity of water resource was defined as average annual runoff by a simple Tank model with three serial tanks, and water demand for public, industrial, agricultural and the other uses was determined using the per-unit method employed in the Water Vision 2020, the Ministry of Construction and Transportation. The results showed that total amount of water resources in the Namgang sub-basin was estimated as about $935,414{\times}10^{3}m^3/yr$ for a 10-year period from 2000 to 2009 and the water withdrawals in public, industrial, agricultural and other sector were derived as $105,493{\times}10^{3}m^3/yr$, $32,686{\times}10^{3}m^3/yr$, $243,194{\times}10^{3}m^3/yr$, and $81,615{\times}10^{3}\;m^3m^3/yr$, respectively. In addition, the Namgaram Inno-city project could increase the overall water demand by $17,156{\times}10^{3}\;mm^3/yr$ due to the population influx.

Development of Convergence Education Program Based on 3D Panorama Virtual Fieldwork Courses on Water Spider in Eundaeri (은대리 물거미 서식지의 3D 파노라마 가상야외학습장 융합교육 프로그램 개발)

  • Yoon, Ma-Byong
    • The Journal of the Korea Contents Association
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    • v.19 no.10
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    • pp.607-619
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    • 2019
  • This study used Natural Monument water spiders and their habitats as educational contents, so those students could have a scientific literacy and the beautiful memories of Eundaeri's marshes through developing a virtual fieldwork courses (VFC) and observing the ecology of water spiders. In order to develop the program, the 2015 revised national curriculum and its textbooks were analyzed. In accordance with the STEAM model, we developed teaching-learning materials for 7 classes. Students produced 3D panorama virtual fieldwork courses (PVFC) about water spider by team-based cooperative learning, enabling them to emotionally experience the meaning and value of water spiders. A panel of six education experts verified the validity of the program and found it to be fairly valid at 4.24 (CVI = .88) on the 5-point Likert scale. In order to confirm the suitability of the program, students in the middle school science clubs participated in pilot testing camp. Their average classes satisfaction was 4.24 and students were very satisfied with the usefulness of the program, the fresh learning contents, and the suitability of the convergence education class. This study could contribute to convergence education related to ecology and virtual reality for adolescents.