• 상하수도학회지
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• 제22권6호
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• pp.673-679
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• 2008

The representative microorganism of autotrophic denitrification using sulfur granule, oxidizes the reduction from S and performs denitrification by reducing $NO_3{^-}-N$ to $N_2$ gas. The sampling of autotrophic denitrification microorganisms has been performed from foreshore sludge, condensed sludge, and active sludge, but the analysis of autotrophic denitrification microbial community characteristics has been lacking. Based on the separation and identification of each sample using the PCR and DGGE methodologies, many types of sulfuric microorganisms and autotrophic denitrification microorganisms were found.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2007년도 학술발표회 논문집
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• pp.46-52
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• 2007

As flue gas desulfurization (FGD) wastewater contains high concentrations of nitrate and is very low in organic carbon, the feasibility of nitrate removal by autotrophic denitrification using Thiobacillus denitrificans was studied. This autotrophic bacteria oxidizes elemental sulfur to sulfate while reducing nitrate to elemental nitrogen gas, thereby eliminating the need for addition of organic compounds such as methanol. Owing to the unusually high concentrations of dissolved salts $(Ca^{2+},\;Mg^{2+},\;Na^+,\;K^+,\;B^+,\;SO_4^{2-},\;Cl^-,\;F^-,)$ in the FGD wastewater, extensive laboratory-scale and pilot-scale tests were carried out in sulfur-limestone reactors (1) to determine the effect of salinity on autotrophic denitrification, (2) to evaluate the use of limestone for pH control and as source of inorganic carbon for microbial growth, and, (3) to find the optimum environmental and operational conditions for autotrophic denitrification of FGD wastewater. The experimental results demonstrated that (1) autotrophic denitrification is not inhibited up to 1.8 mol total dissolved salt content; (2) inorganic carbon and inorganic phosphorus must be present in sufficiently high concentrations; (3) limestone can supply effective buffering capacity and inorganic carbon; (4) the high calcium concentration may interfere with pH control, phosphorus solubility and limestone dissolution, hence requiring pretreatment of the FGD wastewater; and, 5) under optimum conditions, complete autotrophic denitrification of FGD wastewater was obtained in a sulfur-limestone packed bed reactor with a sulfur:limestone volume ratio of 2:1 for volumetric loading rates up to 400g $NO_{3^-}N/m^3.d$. The interesting interactions between autotrophic denitrification, pH, alkalinity, and the unusually high calcium and boron content of the FGD wastewater are highlighted. The engineering significance of the results is discussed.

• 한국유기농업학회:학술대회논문집
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• 한국유기농업학회 2004년도 하반기 학술발표대회
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• pp.34-51
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• 2004

• 상하수도학회지
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• 제18권2호
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• pp.121-127
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• 2004

It is necessary to supply external carbon source for enhancement of biological nitrogen removal from domestic wastewater with low influent C/N ratio. Sulfide was chosen as a cost effective electron donor and reaction stoichiometry for autotrophic denitrification was investigated by conducting bench-scale experiments in this study. Higher sulfur to nitrogen (S/N) ratio than the calculated value from theoretical reaction stoichiometry was required when the anoxic reactor was operated at open condition because dissolved oxygen introduced by surface aeration reacted with sulfide with ease. In addition, higher sulfate production and lower yield of microorganism could be observed under the same condition. It was possible to obtain reliable reaction stoichiometry for autotrophic denitrification by establishing pure anoxic condition. Linear relationship between bacterial growth and consumption of nitrate, sulfide, alkalinity, and sulfate production enabled to derive a relatively correct reaction stoichiometry for autotrophic denitrification when sulfide was used as an electron donor.

• 한국환경농학회:학술대회논문집
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• 한국환경농학회 2011년도 30주년 정기총회 및 국제심포지엄
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• pp.296-296
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• 2011

• 식물조직배양학회지
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• 제28권3호
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• pp.117-121
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• 2001

배양환경을 달리하여 생산한 3가지의 유묘 (타가영양묘, 혼합영양묘, 자가영양묘)를 대상으로 잎의 형태적 특성 및 탄수화물 함량 등을 비교 분석한 결과, 온실묘, 자가영양묘와 혼합영양묘는 하표피층에 왁스의 결정형이 관찰되었으나 타가영양묘에서도 관찰되지 않았다. 기공수와 크기에 있어서는 타가영양묘가 자가영양묘에 비하여 기공이 크고 많았다. 특히, 자가영양묘의 기공형태와 크기는 온실에서 자란 유묘와 거의 유사하였다. 식물체의 탄수화물함량은 자가영양묘가 혼합영양과 타가영양묘에 비하여 많았으며 유리당도 역시 자가영양묘가 가장 많았고 모든 배양묘에서 glucose의 함량이 가장 많았다.

• Journal of Microbiology and Biotechnology
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• 제15권3호
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• pp.455-460
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• 2005

A pilot-scale sulfur particle autotrophic denitrification (SPAD) process for the treatment of municipal wastewater was operated for 10 months at Shihwa, Korea, and higher than $90\%\;NO^{-}_{3}-N$ removal efficiency was observed. Plate counting showed that the lower part of the denitrifying column reactor had the most autotrophic denitrifiers. The biofilm thickness formed on sulfur particles from the SPAD reactor was approximately $25-30\;{\mu}m$, measured by DAPI (4,6-diamidino-2-phenylindole) staining. The presence of bacteria inside the highly porous sulfur particle was also monitored by SEM observation of the internal surfaces of broken sulfur particles. Biofilm extracellular polymeric substances (EPS) analysis showed that the ratio of carbohydrate to protein decreased with the reactor heights at which biofilm-formed sulfur particles were obtained.

• Environmental Engineering Research
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• 제10권6호
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• pp.283-293
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• 2005

Characteristics of sulfur-based autotrophic denitrification in a semi-continuous type reactor and the kinetic parameters were studied. Enriched autotrophic denitrifying culture was used for the reactor operation. Biomass growth on sulfur particles and in the liquid medium was monitored using the DAPI staining method. From the result of ion concentration changes and the biomass growth, maximum specific growth rate, ${\mu}_{max}$, and the half velocity constant, $K_M$, were estimated as $0.61\;d^{-1}$ and 3.66 mg/L, respectively. Growth yield coefficient, Y values for electron acceptor and donor were found as 0.49 gVSS/g N and 0.16 gVSS/g S. The biomass showed specific denitrification rate, ranging 0.86-1.13 gN/g VSS-d. A half-order equation was found to best simulate the denitrification process in the packed bed reactor operated in the semi-continuous mode.

• Environmental Engineering Research
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• 제24권2호
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• pp.309-317
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• 2019

This study evaluated the kinetics of acrylamide (AM) biodegradation by mixed culture bacteria and Enterobacter aerogenes (E. aerogenes) in sequencing batch reactor (SBR) systems with AQUASIM and linear regression. The zero-order, first-order, and Monod kinetic models were used to evaluate the kinetic parameters of both autotrophic and heterotrophic nitrifications and both AM and chemical oxygen demand (COD) removals at different AM concentrations of 100, 200, 300, and 400 mg AM/L. The results revealed that both autotrophic and heterotrophic nitrifications and both AM and COD removals followed the Monod kinetics. High AM loadings resulted in the transformation of Monod kinetics to the first-order reaction for AM and COD removals as the results of the compositions of mixed substrates and the inhibition of the free ammonia nitrogen (FAN). The kinetic parameters indicated that E. aerogenes degraded AM and COD at higher rates than mixed culture bacteria. The FAN from the AM biodegradation increased both heterotrophic and autotrophic nitrification rates at the AM concentrations of 100-300 mg AM/L. At higher AM concentrations, the FAN accumulated in the SBR system inhibited the autotrophic nitrification of mixed culture bacteria. The accumulation of intracellular polyphosphate caused the heterotrophic nitrification of E. aerogenes to follow the first-order approximation.

• Journal of Microbiology and Biotechnology
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• 제22권8호
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• pp.1155-1160
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• 2012

Sulfur-utilizing autotrophic denitrification relies on an inorganic carbon source to reduce the nitrate by producing sulfuric acid as an end product and can be used for the treatment of wastewaters containing high levels of nitrates. In this study, sulfur-denitrifying bacteria were used in anoxic batch tests with sulfur as the electron donor and nitrate as the electron acceptor. Various medium components were tested under different conditions. Sulfur denitrification can drop the medium pH by producing acid, thus stopping the process half way. To control this mechanism, a 2:1 ratio of sulfur to oyster shell powder was used. Oyster shell powder addition to a sulfur-denitrifying reactor completely removed the nitrate. Using 50, 100, and 200 g of sulfur particles, reaction rate constants of 5.33, 6.29, and $7.96mg^{1/2}/l^{1/2}{\cdot}h$ were obtained, respectively; and using 200 g of sulfur particles showed the highest nitrate removal rates. For different sulfur particle sizes ranging from small (0.85-2.0 mm), medium (2.0-4.0 mm), and large (4.0-4.75 mm), reaction rate constants of 31.56, 10.88, and $6.23mg^{1/2}/l^{1/2}{\cdot}h$ were calculated. The fastest nitrate removal rate was observed for the smallest particle size. Addition of chemical oxygen demand (COD), methanol as the external carbon source, with the autotrophic denitrification in sufficiently alkaline conditions, created a balance between heterotrophic denitrification (which raises the pH) and sulfur-utilizing autotrophic denitrification, which lowers the pH.