• 한국한의학연구원논문집
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• 제1권1호
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• pp.471-476
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• 1995

Natural products used for oriental medicine often come from various geographical sources, after several different distribution channels. Therefore some form of quality control procedure is required to safeguard naturl products for prescriptions purposes. To achieve this, systematic apprroaches such as morphological examination, microscopic analysis of powdered herbs and chemical analysis can be carried out. However, to ensure absolute criteria for quality assurance of natural products, DNA fingerprinting method such as RAPD(Random amplified polymorphism DNA) analysis can be used for authentication of natural products for authenticatin of natural products. In this study, warious oligonucleotide primers will be synthesized for the detection of RAPD markers and also parameters of affecting PCR(Polymerase Chain Reaction) in the detection of RAPD markers of rare and high-priced natural products will be studied with genomic DNA of chosen samples.

• Natural Product Sciences
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• 제21권3호
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• pp.185-191
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• 2015

Comparative molecular analysis has been frequently adopted for the authentication of herbal medicines as well as the identification of botanical origins. Roots and rhizomes of the family Umbelliferae have been used as traditional herbal medicines to relieve various symptoms such as inflammation, neuralgia and paralysis in countries of East Asia. Since most herbal medicines of the Umbelliferae roots or rhizomes are generally supplied in the form of dried slices, morphological examination does not often provide sufficient evidence to identify the botanical origin. Using species-specific probes developed by the comparative analysis of nrDNA ITS sequences, a DNA chip was developed to identify herbal medicines for 13 species in the Umbelliferae. The developed DNA Chip proves its potential as a rapid, sensitive and effective tool for authenticating herbal medicines in future.

• Advances in Traditional Medicine
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• 제5권4호
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• pp.262-271
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• 2005

Cordyceps, one of the most valued traditional Chinese medicines, consists of the dried fungus Cordyceps sinensis growing on the larva of caterpillar. It is also known as 'winter-worm and summer-grass' because of its appearance during different seasons. The parasitic complex of the fungus and the caterpillar is found in soil of a prairie at an elevation of 3,500 to 5,000 meters in northwestern part of China. According to Chinese medicinal theory, Cordyceps is used to replenish the kidney and soothe the lung, and indeed many clinical applications have been reported. The natural Cordyceps is rare and expensive on the local market, and therefore, several mycelial strains have been isolated from natural Cordyceps and manufactured in large quantities by fermentation technology, and they are commonly sold as health food products in Orient. The adulterants of Cordyceps are commonly found on the market, and therefore the authentication of these products has to be defined. Having the urgent need from current market, different chemical markers such as nucleoside, ergosterol, mannitol and polysaccharide are being used for quality control of Cordyceps. Unfortunately, these markers are far from optimization, and therefore extensive works are needed to define the pharmacological efficiency of these markers.

• 한국토양비료학회지
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• 제30권2호
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• pp.189-195
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• 1997

본 실험은 인산염 분해균을 밀의 근권토양으로부터 분리 동정하고 인산염 분해균의 유기산 생성과 pH와의 관계를 조사하기 위해 실시하였다. 인산염 분해균은 36시간 배양후 선명한 투명대(clear zone)를 형성하였다. API 20E System과 BIOLOG$^{TM}$ analysis를 사용하여 동정한 결과 이 균주는 Entrobacter agglomerans로 동정되었다. Similarity와 distance는 각자 0.656과 4.790로 나타났다. Hydroxyapatite를 함유한 배지에서 E. agglomerans를 배양하는 동안 인산의 농도가 현저히 증가하였으며, pH와 인산의 농도와는 고도의 역상관($r^2=0.933$)을 보였다. HPLC로 분석한 결과 이 균주는 여러 가지 유기산을 생성하였으며 그 중 oxalic acid가 가장 많이 생성되었다. Acid phosphatase는 alkaline phosphatase에 비해서 10-15배의 활성을 보였으며, alkaline phosphatase는 배양 동안 거의 0에 가까운 활성을 보였다. E. agglomerans의 population은 배양 하루 동안 현저히 증가하였으나 그 후 급격히 감소하였다.