• 한국식품영양학회지
• /
• 제12권3호
• /
• pp.233-239
• /
• 1999

To develop the potential use as new host strain for gene cloning the optimal conditions for transform-ation of alkali-tolerant Bacillus sp. were examined. The Bacillus sp. YA-14 was cultured to late logarith-mic growth phase at 37$^{\circ}C$ in modified SPI medium (pH8.0) containing 0.4% MgSO4 0.5mM CaCl2 1 ml of competent cell was mixed with 0.5$\mu$g of plasmid DNA and incubated with shaking at 37$^{\circ}C$ for 40min. The transformation frequency under the optimal condition was 4.53$\times$10-6 CFU/ml/g plasmid DNA. The electrophresis and stably maintained in the new host.

• 한국안전학회지
• /
• 제7권2호
• /
• pp.47-56
• /
• 1992

The retardation effect of fatigue crack propagation after cyclic overloading seems to be affected by strain hardening exponent. Namely, for the material with high values of n, the delay effect is found to be severe. We proposed a modified crack retardation equation which may apply the retardation of fatigue crack growth after a cyclic overloading, as (da/dN)'$_{cyc}$=($\mu$n＋λ)B $\Delta$ $K^{q}$ /[(1－ $R_{eff}$) $K_{cf}$ －$\Delta$K]. where, $R_{eff}$ is effective stress ratio [＝( $K_{min}$－K, os)/( $K_{max}$－ $K_{res}$)] The constants $\mu$＝－0.5 and λ＝0.6, and the values are found to be identical for materials such as aluminum (A 1060), steel (SS 34), brass ( $B_{s}$ SIB) and stainless steel (SUS 304) used in this investigation. (SUS 304) used in this investigation.ation.n.n.

• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2002년도 추계종합학술대회
• /
• pp.597-600
• /
• 2002

새 천년이 시작하면서 자본주의의 새로운 기업 모델로 e-Biz가 각광받았다. 하지만 곧바로 사양되었으며, 이 시점에서 새로운 대안으로 떠오른 것이 바로 모바일 인터넷 비즈니스였다. M-Biz는 편재성(Ubiquity), 접근성(Reachability), 보안성(Security), 위치확인(Localiz ation), 즉시 연결성(Instant Connectivity), 개인화(Personalization)의 모바일 인터넷 장점을 이용하여 뚜렷한 수익모델로 가능해졌기 때문이다. 이런 모바일 컨텐츠 서비스는 정보제공(Information), Entertainment, 상거래(Transaction), 단문메시지 (Communication) 등을 현재 제공하고 있으며, 이러한 기능들을 현재 개발되고 있는 홈 오토메이션 시스템과 결합하여 새로운 형태의 홈 오토메이션을 기반으로 하는 모바일 서비스 지원 기법을 본 논문에서는 논하였다.

• 산업공학
• /
• 제10권3호
• /
• pp.179-187
• /
• 1997

The efficiency of material flow systems in terms of optimal network flow and minimum cost flow has always been an important design and operational goal in material handling and distribution system. In this research, an attempt was made to develop a new algorithm and the model to solve a stochastic material flow network with bidirectional and uncertain flows. A stochastic material flow network with bidirectional flows can be considered from a finite set with unknown demand probabilities of each node. This problem can be formulated as a special case of a two-stage linear programming problem which can be converted into an equivalent linear program. To find the optimal solution of proposed stochastic material flow network, some terminologies and algorithms together with theories are developed based on the partitioning and subgradient techniques. A computer program applying the proposed method was developed and was applied to various problems.

• KSBB Journal
• /
• 제6권4호
• /
• pp.337-344
• /
• 1991

The liquid-liquid extraction of lipase A from Candide cylindracea and lipase B from porcine pancrease was carried out using reversed micellar organic solvents. Effects of various factors such as ionic strength, pH, and species and concentration of surfactant, on lipase solubilization were studied. A cationic surfactant cetyl-trimethyl ammonium bromide (CTAB) in isooctane/nhexanol(1:1) was found to be an effective solvent and its optimum concentration was 50 mM. KCl among various salts tested was the most effective and the efficiency of solubilization of lipase increased with decreasing the ionic strength of salts. The maximum activity and solubiliz ation of protein were obtained at pH 8. The stripping efficiency has a maximum value at pH 4 and increases with KCl concentration in the range of 0.2∼1.0 M. After the solubilization and stripping, the overall recovery efficiency of mass and specific activity of lipases was 62% and 66%, respectively.

• 대한의생명과학회지
• /
• 제24권3호
• /
• pp.150-156
• /
• 2018

Tankyrases are multifunctional poly (ADP-ribose) polymerases that regulate a variety of cellular processes including WNT signaling, telomere maintenance, regulation of mitosis, and many others. Tankyrases interact with target proteins and regulate their interactions and stability through poly (ADP-ribosyl) ation. In addition to their roles in telomere maintenance and regulation of mitosis, tankyrase proteins regulate tumor suppressors such as AXIN, PTEN, and AMOT. Therefore, tankyrases can be effective targets for cancer treatment. Tankyrase inhibitors could affect a variety of pathways that are carcinogenic (essential for the unlimited proliferation of human cancer cells), including WNT, AKT, YAP, telomere maintenance, and regulation of mitosis. Recently, new aspects of the function and mechanism of tankyrases have been reported and several tankyrase inhibitors have been identified. Also, it has been proposed that the combination of conventional chemotherapy agents with tankyrase inhibitors may have synergistic anti-cancer effects. Based on this, it is expected that more advanced and improved tankyrase inhibitors will be developed, enabling new therapeutic strategies against cancer and other tankyrase linked diseases. This review discusses tankyrase function and the role of tankyrase inhibitors in the treatment of cancer.

• 전자공학회논문지A
• /
• 제30A권3호
• /
• pp.67-75
• /
• 1993

In many practical applications of systolic array, it is common that the problem size(n) is larger than the array size(M). In this case, the problem has to be partitioned into block to fit into the array before it is processed. This paper presents a problem partition method for dynamic programming and 2-dimensional systolic array suitable for it. Designed array has two types of array configur-ation for processing the partitioned problem. The queue is designed for storing and recirculating the intermediate results in the correct location and time. The number of processing elements and queues required are M(3M+1)/2, 4M respectively. The total processing time is 2(M+1)+(n+10M+3)(n/M-1)(n/M-1)/6.

• 자원환경지질
• /
• 제32권6호
• /
• pp.661-667
• /
• 1999

Estimation of inflow rates into subsection of a tunnel is establishing the proposed grouting part, measuring the degree of grouting, and settling the dispute over deplrtion of groundwater which may be resulted from tunneling. A current meter was used to calculate inflow rates of groundwater to each subsection of the tunnel. The study area is composed of section 1 and 2 of Imha-Youngchun waterway trnnel which has 32.976km length, with each section having 3,745m and 4,079m, respectively. The depth from groung surface to tunnel ranges from 122.45m to 358.3m. Total inflow rates of groundwater into each section measured three times by the current meter, together with bottle and eye measurement, were compared with groundwater inflow rates of each section measured by datalogger. The calcuated inflow rates of the sections by bottle and eye measurement were 8.8%∼54.7% of inflow rate (averaging 27,4%), whwewas those by the current meter were 76.9%∼110.6%(averaging 92.9%). Therfore, the current meter is regarded as useful method to calculate groundwater inflow rates into subsections of a tunnel.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
• /
• pp.63-65
• /
• 2003

The purpose of the study is to produce the surface ground temperature diagnostically using surface EBM with the use of GRID model in Geographic Information Systems (GIS). Certain characteristics have been analyzed for local slope effect, coastal effect and influence of high orographic aspect on the surface ground temperature. We present discussions on the meteorological responsibility for their temperature. The derived surface ground temperatures can be provided for comparison with those from satellite-based observ ation.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
• /
• pp.866-868
• /
• 2003

In this paper, we developed those Directory Services based on the Web-Service, because Web-Service environments provide a suitable method to gather requested information in an appropriate way. The proposed architecture cooperate with other OpenLS(Open Location Service) Core Services ( Location Utility Service and Router Determin ation service) and is an interoperability one - it ident ifies those global elements of the global Web-Services network that are required in order to ensure interoperability between Web-Services. In this paper, a new architecture of Directory Service with OpenLS Core Services is proposed and tested in OpenLS Core Services environments.