• Membrane Journal
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• v.31 no.6
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• pp.434-442
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• 2021

Poly(vinylidene fluoride) (PVDF) membrane has a good membrane durability because of its high mechanical resistance, thermal and chemical stability. However, the strong hydrophobic property of PVDF membrane can induce a low water permeability and easy fouling by proteins and organic matters. In order to improve the anti-fouling properties of PVDF membrane, the PVDF mixed matrix asymmetric membranes impregnated with biofunctional material 𝛽-cyclodextrin (𝛽-CD) in the membrane structure were prepared by phase inversion method. The membrane filtration experiments of pure water and BSA solution were performed using the PVDF/𝛽-CD mixed matrix asymmetric membranes prepared according to the 𝛽-CD contents. The experiments showed that the introduction of 𝛽-CD into the PVDF polymer matrix contributed to increase in the hydrophilic property of the PVDF membranes, and this led to the reduction of contact angles and improvement of anti-fouling properties. The PVDF/𝛽-CD membrane which was prepared using the dope solution with a 2 wt% 𝛽-CD content represented 64 L/m²·h of pure water flux, 95% of BSA rejection and maximum 80% of flux enhancements compared to flux results of the pristine PVDF membrane.

• Journal of Microbiology and Biotechnology
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• v.29 no.3
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• pp.373-381
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• 2019

Site-directed mutagenesis was employed to generate five different triple point mutations in the double mutant (C295A/I86A) of Thermoanaerobacter ethanolicus alcohol dehydrogenase (TeSADH) by computer-aided modeling with the aim of widening the small alkyl-binding pocket. TeSADH engineering enables the enzyme to accept sterically hindered substrates that could not be accepted by the wild-type enzyme. The underline in the mutations highlights the additional point mutation on the double mutant TeSADH introduced in this work. The catalytic efficiency ($k_{cat}/K_M$) of the ${\underline{M151A}}$/C295A/I86A triple TeSADH mutant for acetophenone increased about 4.8-fold higher than that of the double mutant. A 2.4-fold increase in conversion of 3'-methylacetophenone to (R)-1-(3-methylphenyl)-ethanol with a yield of 87% was obtained by using ${\underline{V115A}}$/C295A/I86A mutant in asymmetric reduction. The ${\underline{A85G}}$/C295A/I86A mutant also produced (R)-1-(3-methylphenyl)-ethanol (1.7-fold) from 3'-methylacetophenone and (R)-1-(3-methoxyphenyl)-ethanol (1.2-fold) from 3'-methoxyacetophenone, with improved yield. In terms of thermal stability, the ${\underline{M151A}}$/C295A/I86A and ${\underline{V115A}}$/C295A/I86A mutants significantly increased ${\Delta}T_{1/2}$ by $+6.8^{\circ}C$ and $+2.4^{\circ}C$, respectively, with thermal deactivation constant ($k_d$) close to the wild-type enzyme. The ${\underline{M151A}}$/C295A/I86A mutant reacts optimally at $70^{\circ}C$ with almost 4 times more residual activity than the wild type. Considering broad substrate tolerance and thermal stability together, it would be promising to produce (R)-1-(3-methylphenyl)-ethanol from 3'-methylacetophenone by ${\underline{V115A}}$/C295A/I86A, and (R)-1-phenylethanol from acetophenone by ${\underline{M151A}}$/C295A/I86A mutant, in large-scale bioreduction processes.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.18 no.6
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• pp.389-394
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• 2017

In a conventional ship structure, stiffeners with an asymmetric section, such as inverted angles, are used widely despite the disadvantage of strength compared to the stiffeners with a symmetric section, such as a built-up T. On the other hand, T-type built-up members are attracting more attention than L-type inverted angles due to the increased size of ships. The purpose of this study was to develop an optimal design program for a built-up T, and apply an evolution strategy as an optimization technique. In the optimization process, the gross thickness concept was adopted for the design variables and objective function, and the constraints are set up based on HCSR (Harmonized Common Structural Rules). Using the developed program in this study, the optimal stiffener design was carried out for 300K VLCC and 158K COT of which the orders were obtained lately. The optimal results revealed the weight reduction effect of 144 tons and 60 tons, respectively.

• Steel and Composite Structures
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• v.34 no.5
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• pp.699-713
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• 2020

The aerodynamic performance of long-span cable-stayed bridges is much dependent on its geometrical configuration and countermeasure strategies. In present study, the aerodynamic performance of three composite cable-stayed bridges with different tower configurations and passive aerodynamic countermeasure strategies is systematically investigated by conducting a series of wind tunnel tests in conjunction with theoretical analysis. The structural characteristics of three composite bridges were firstly introduced, and then their stationary aerodynamic performance and wind-vibration performance (i.e., flutter performance, VIV performance and buffeting responses) were analyzed, respectively. The results show that the bridge with three symmetric towers (i.e., Bridge I) has the lowest natural frequencies among the three bridges, while the bridge with two symmetric towers (i.e., Bridge II) has the highest natural frequencies. Furthermore, the Bridge II has better stationary aerodynamic performance compared to two other bridges due to its relatively large drag force and lift moment coefficients, and the improvement in stationary aerodynamic performance resulting from the application of different countermeasures is limited. In contrast, it demonstrates that the application of both downward vertical central stabilizers (UDVCS) and horizontal guide plates (HGP) could potentially significantly improve the flutter and vortex-induced vibration (VIV) performance of the bridge with two asymmetric towers (i.e., Bridge III), while the combination of vertical interquartile stabilizers (VIS) and airflow-depressing boards (ADB) has the capacity of improving the VIV performance of Bridge II.

• Journal of Korea Water Resources Association
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• v.43 no.10
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• pp.843-850
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• 2010

By employing multi-directional random waves, a parameter controlling the force acting on a long caisson breakwater is investigated in detail. Both JONSWAP (Joint North Sea Wave Project) and asymmetric directional spectra are adopted for frequency and directional spectra. It is found that the parameter decreases as the length of caisson and the angle of main direction of incident waves increase. Furthermore, the parameter is much similar to that of regular waves as the maximum spreading parameter $s_{max}$ increases. The parameter, however, decreases as asymmetry parameter ${\mu}$ increases when the main direction of incident waves is oblique to the breakwater.

• Journal of Microbiology and Biotechnology
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• v.23 no.3
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• pp.343-350
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• 2013

(R)-[3,5-Bis(trifluoromethyl)phenyl] ethanol is a key chiral intermediate for the synthesis of aprepitant. In this paper, an efficient synthetic process for (R)-[3,5- bis(trifluoromethyl)phenyl] ethanol was developed via the asymmetric reduction of 3,5-bis(trifluoromethyl) acetophenone, catalyzed by Leifsonia xyli CCTCC M 2010241 cells using isopropanol as the co-substrate for cofactor recycling. Firstly, the substrate and product solubility and cell membrane permeability of biocatalysts were evaluated with different co-substrate additions into the reaction system, in which isopropanol manifested as the best hydrogen donor of coupled NADH regeneration during the bioreduction of 3,5-bis(trifluoromethyl) acetophenone. Subsequently, the optimization of parameters for the bioreduction were undertaken to improve the effectiveness of the process. The determined efficient reaction system contained 200mM of 3,5-bis(trifluoromethyl) acetophenone, 20% (v/v) of isopropanol, and 300 g/l of wet cells. The bioreduction was executed at $30^{\circ}C$ and 200 rpm for 30 h, and 91.8% of product yield with 99.9% of enantiometric excess (e.e.) was obtained. The established bioreduction reaction system could tolerate higher substrate concentrations of 3,5- bis(trifluoromethyl) acetophenone, and afforded a satisfactory yield and excellent product e.e. for the desired (R)-chiral alcohol, thus providing an alternative to the chemical synthesis of (R)-[3,5-bis(trifluoromethyl)phenyl] ethanol.

• Journal of Microbiology and Biotechnology
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• v.28 no.4
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• pp.613-621
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• 2018

In this study, interface-assembled carbonyl reductase (IACR) was prepared and used in the synthesis of S-licarbazepine in a toluene/Tris-HCl biphasic system. The carbonyl reductase (CR) was conjugated with polystyrene to form a surfactant-like structure at the interface of the toluene/Tris-HCl biphasic system. The interface-assembled efficiency of IACR reached 83% when the CR (180 U/mg) and polystyrene concentration were $8{\times}10^2g/ml$ and $3.75{\times}10^3g/ml$, respectively. The conversion reached 95.6% and the enantiometric excess of S-licarbazepine was 98.6% when $3.97{\times}10^6nmol/l$ oxcarbazepine was converted by IACR using 6% ethanol as a co-substrate in toluene/Tris-HCl (12.5:10) at $30^{\circ}C$ and $43{\times}g$ for 6 h. IACR could be reused efficiently five times.

• Journal of ILASS-Korea
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• v.12 no.1
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• pp.11-17
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• 2007

The swirl spray for direct-injection spark-ignition (DISI) engines was investigated using a nozzle whose exit surface shape was cut with a certain tapered angle. The reason for the change in spray's characteristics at various tapered angles was explained by the data correlating the taper and flow angles. The spray tended to shift its characteristics from the symmetric to asymmetric when the tapered angle was increased; furthermore, the spray penetration and spray cone angle were also increased. When the tapered angle was greater than the $90^{\circ}$ minus flow angle, an opened hollow cone spray was formed because of the fuel impingement against the tapered surface area of the nozzle exit. This behavior indicates that the reduction in the air pressure difference between the inner and outer spray of the spray can be achieved. This behavior also promises the potential use of the tapered nozzle for the case where the independence of the spray performance from atmospheric pressure and fuel temperature is desired.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.39 no.5
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• pp.87-94
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• 2002

In this paper an asynchronous away multiplier with a parallel array structure is introduced. This parallel array structure is used to make the computation time faster with a lower Power consumption. Asymmetric parallel away structure is used to minimize the average computation time in an asynchronous multiplier. Simulation shows that this structure reduces the time needed for computation by 55% as compared to conventional booth encoding array structures and that the multiplier with the proposed away structure shows a reduction of 40% in the computational time with a relatively lower power consumption.

• Membrane and Water Treatment
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• v.11 no.4
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• pp.237-245
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• 2020

The effects of the mixed two solvents, Dimethylacetamide (DMAc) and Dimethylformamide (DMF), and Polyethylene glycol (PEG) and Polyvinylpyrrolidone (PVP) as additives on performance of Polyvinylidene fluoride (PVDF) membranes were studied. Initially, PEG200 was used as a primary additive at fixed percentage of 5% wt. PVP was then blended with PEG200 in different concentrations. PVDF and DMAc were used as polymer and solvent in the casting solutions, respectively. To control the diffusion rate of PVP in the presence of PEG200 and PVP blend, mixtures of DMAc and DMF were used as the mixed solvent in the casting solutions. Asymmetric PVDF membranes were prepared via phase inversion process in a water bath and the effects of two additives and two solvents on the membrane morphology, pure water flux (PWF), hydrophilicity and rejection (R) were investigated. Attenuated Total Reflection Fourier Transform Infrared Spectra (ATR-FTIR) analysis was used to show the residual PVP on the surface of the membranes. Atomic Force Microscopy (AFM) was utilized to determine roughness of membrane surface. The use of mixed solvents in the casting solution resulted in reduction of PVP diffusion rate and increment of PEG diffusion rate. Eventually, PWF and R values reduced, while porosity and hydrophilicity increased.