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Novel method of histopathological analysis after testicular sperm extraction in patients with nonobstructive and obstructive azoospermia

  • Cito, Gianmartin;Coccia, Maria Elisabetta;Picone, Rita;Nesi, Gabriella;Cocci, Andrea;Dabizzi, Sara;Garaffa, Giulio;Fucci, Rossella;Falcone, Patrizia;Bertocci, Francesco;Santi, Raffaella;Criscuoli, Luciana;Serni, Sergio;Carini, Marco;Natali, Alessandro
    • Clinical and Experimental Reproductive Medicine
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    • 제45권4호
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    • pp.170-176
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    • 2018
  • Objective: To assess whether the "testicular pool" could be used for histological analysis and whether it gave more accurate information than the standard testicular biopsy. Methods: Between January 2017 and March 2018, this single-center prospective study included 60 azoospermic men undergoing conventional bilateral testicular sperm extraction. Six samples were excised from each testicle and transferred to an embryologist. One additional biopsy was randomly taken from each testis for a histological analysis. After processing, the testicular pool was also sent for a histological analysis, which showed normal spermatogenesis (NS), hypospermatogenesis (HYPO), maturation arrest (MA), Sertoli cell-only syndrome (SCOS), and tubular atrophy (TA). Results: Twenty of the 60 patients (33.3%) had obstructive azoospermia (OA), while the remaining 40 (66.6%) had nonobstructive azoospermia. Their mean age was 40.5 years. All patients with OA had previously undergone unsuccessful testicular fine-needle aspiration. Successful sperm retrieval (SSR) occurred in 93.3% of patients. Histological analysis of the testicular biopsy revealed NS in 12 patients (20%), HYPO and TA in 28 patients (46.6%), MA in eight patients (13.3%), and SCOS in 12 patients (20%). The testicular pool analysis showed NS in 12 patients (20%), HYPO and TA in 44 patients (73.3%), MA in four patients (6.6%), and SCOS in no patients. In four patients with MA (6.6% of the total sample) and 12 patients with SCOS (20% of the total sample) according to the standard testicular biopsy, the embryologist found SSR with cryopreservation. Overall, in 44 patients (73.3%), the testicular pool analysis confirmed the histological findings of the standard testicular biopsy. In the 16 cases (26.6%) with a discrepancy between the single-biopsy histological findings and SSR, the testicular pool analysis confirmed the embryological data on SSR. Conclusion: The testicular pool proved to be easily analyzable, practical, manageable, and more accurate for predicting sperm retrieval than standard testicular biopsy.

Comparison of Long-Term Angiographic Results of Wide-Necked Intracranial Aneurysms : Endovascular Treatment with Single-Microcatheter Coiling, Double-Microcatheter Coiling, and Stent-Assisted Coiling

  • Kim, Hyun Sik;Cho, Byung Moon;Yoo, Chan Jong;Choi, Dae Han;Hyun, Dong Keun;Shim, Yu Shik;Song, Joon Ho;Oh, Jae Keun;Ahn, Jun Hyong;Kim, Ji Hee;Chang, In Bok
    • Journal of Korean Neurosurgical Society
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    • 제64권5호
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    • pp.751-762
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    • 2021
  • Objective : Endovascular treatment of intracranial aneurysms is challenging in case of wide-necked aneurysms because coils are prone to herniate into the parent artery, causing thromboembolic events or vessel occlusion. This study aims to compare long-term angiographic results of wide-necked aneurysms treated by stent-assisted, double-microcatheter, or single-microcatheter groups. Methods : Between January 2003 and October 2016, 108 aneurysms that were treated with endovascular coil embolization with a neck size wider than 4 mm and a follow-up period of more than 3 years were selected. We performed coil embolization with single-microcatheter, double-microcatheter, and stent-assisted techniques. Angiographic results were evaluated using the Raymond-Roy occlusion classification (RROC). All medical and angiographic records were reviewed retrospectively. Results : Clinical and angiographic analyses were conducted in 108 wide-necked aneurysms. The immediate post-procedural results revealed RROC class I (complete occlusion) in 66 cases (61.1%), class II (residual neck) in 36 cases (33.3%), and class III (residual sac) in six cases (5.6%). The final follow-up results revealed class I in 48 cases (44.4%), class II in 49 cases (45.4%), and class III in 11 cases (10.2%). Of a total of 45 (41.6%) radiologic recurrences, there were 21 cases (19.4%) of major recurrence that required additional treatment, and 24 cases (22.2%) of minor recurrence. The final follow-up angiographic results showed statistically significant differences between the stent-assisted group and the others (p<0.01). Conclusion : Long-term follow-up angiography demonstrated that the stent-assisted technique had a better complete occlusion rate than the other two techniques.

Novel Design Conditions to Optimize Power Coupling in Optical Grating-Assisted Directional Couplers

  • Ho, Kwang-Chun;Kong, Hyung-Yun
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2002년도 ITC-CSCC -1
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    • pp.508-511
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    • 2002
  • By defining a power distribution ratio (PDR) and coupling efficiency (CE) amenable to the rigorous analytical solutions of newly developed rigorous modal transmission-line theory (MTLT), we explicitly analyze the power coupling characteristics of TE modes propagating in GADCs. The numerical results reveal that the incident power is optimally coupled into the desired guiding channel if the powers of rigorous modes excited at the input boundary of grating-assisted coupler are equally partitioned.

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동심원형 대칭 전기장 집속 방식을 응용한 자가 이온 보조 소스 제작 및 Cu 박막 증착 (Design of Self-ion assisted beam source (SIAB) based on electron focusing with concentric symmetrical electric field and Cu thin film growth by SIAB)

  • 송재훈;김기환;이충만;최성창;송종한;정형진;최원국
    • 한국진공학회지
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    • 제8권2호
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    • pp.121-126
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    • 1999
  • Cu thin film was deposited by a self-ion assisted beam source (SIAB) and the assessment of the Cu films was given. Some characteristics of the source and the experimental procedure are described at various conditions such as total power, ionization efficiency, and ion current vs. deposition rate. The dependence of crystalline structure, impurity concentration, and resistivity of the Cu films deposited by SIAB on acceleration voltage are discussed.

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Environmentally Assisted Crack Growth Behavior of SA508 Cl.3 Pressure Vessel Steel

  • Kim, Jun-Hwan;Kim, In-Sup
    • 한국원자력학회:학술대회논문집
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    • 한국원자력학회 1998년도 춘계학술발표회논문집(2)
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    • pp.154-159
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    • 1998
  • In order to assess the susceptibility of the environmentally assisted cracking(EAC) on SA508 Cl.3 steel in primary water condition, potential step test and slow strain rate test(SSRT) were conducted in a simulated crack tip condition. In this test, anodic dissolution was dominant in the crack tip environments. Proposed simple dissolution model is a modification of Hishida's anodic dissolution model at the plastic zone. One can predict actual crack growth rate with the smooth specimen through this model.

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