• Title/Summary/Keyword: assemblage

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Antimicrobial Active Substances from Entomopathogenic Fungi (Various Applications of Entomopathogenic Fungi)

  • Shin, Tae Young;Woo, Soo Dong;Kim, Jeong Jun
    • 한국균학회소식:학술대회논문집
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    • 2016.05a
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    • pp.13-13
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    • 2016
  • Insects constitute the largest and most diverse group of animals in the world. They also serve as the hosts or nutrient sources for an immense assemblage of pathogens, parasites, and predators. More than 700 fungal species from 100 genera have adopted an entomopathogenic lifestyle. Although entomopathogenic fungi were studied as only biocontrol agents against a variety of pests in various countries, it has been recently focused their additional roles in nature. They are antagonists to/against plant pathogens, endophytes, and possibly even plant growth promoting agents. The potential antimicrobial effect against fungal plant pathogens by an isolate of entomopathogenic fungi including Beauveria bassiana, Lecanicillium spp., and Isaria fumosorosea have been reported since late 1990s, but wasn't reported pathogenicity of the isolate against pests. Later, a Canadian Lecanicillium sp. isolate and L. longisporium isolated from Vertalec$^{(R)}$ showed simultaneous control effect against both aphid and cucumber powder mildew. Therefore, the antimicrobial activities of 342 fungi isolates collected from various regions and conditions in Korea were evaluated against plant pathogenic fungus Botrytis cinerea using dual culture technique on agar plate. As a result, 186 isolates (54.4%) shown the antifungal activity against B. cinerea. The culture filtrates of selected fungi completely suppressed the growth of the microorganisms, indicating that suppression was due to the presence of antimicrobial substances in the culture filtrate. Mode of action of these fungi against insect involves the attachment of conidia to the insect cuticle, followed by germination, cuticle penetration, and internal dissemination throughout the insect. During infection process, secreted enzymes, proteinous toxins, and/or secondary metabolites secreted by entomopathogenic fungi can be used to overcome the host immune system, modify host behavior, and defend host resources. Recently, secondary metabolites isolated from entomopathogenic fungi have been reported as potential bioactive substances. Generally, most of bioactive substances produced by entomopathogenic fungi have reported low molecular weight (lower than 1,000 g/mol) as peptide and, in contrast the high molecular weight fungal bioactive substances are rare. Most substances based on entomopathogenic fungi were shown antimicrobial activity with narrow control ranges. In our study we analyzed the antimicrobial substances having antagonistic effects to B. cinerea. Antimicrobial substances in our fungal culture filtrates showed high thermostability, high stability to proteolytic enzymes, and hydrophilicity and their molecular weights were differed from substance. In conclusion, entomopathogenic fungi showed pathogenicity against insect pests and culture filtrate of the fungi also shown to antimicrobial activity. In the future, we can use the entomopathogenic fungi and its secondary metabolites to control both insect pest control and plant pathogenic fungi simultaneously.

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3D Inversion of Aeromagnetic Data In an Area of Geumsan (금산지구 항공 자력탐사 자료의 3차원 역산)

  • Ko, Kwang-Beom;You, Young-Jun
    • Geophysics and Geophysical Exploration
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    • v.17 no.2
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    • pp.49-57
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    • 2014
  • 3D magnetic inversion, based on the assemblage of 2D forward modeling and inversion as a practical technique to reflect the a priori information, was conducted to investigate the spatial distribution features of black-shale related and pyrometamorphic uranium deposit and several lithological units of Ogcheon Super Group in an area of Geumsan. By using the 3D visualization technique with suitable susceptibility interval and horizontal slice map, the spatial distribution of magnetic susceptibility corresponded to the black shale related uranium bearing lithological units, Black Slate member was well coincided with a information of uranium deposit. Also, even though it is indirect indicator for the detetction of uranium deposits interbedded in Gray Hornfels member, spatial susceptibility distribution which shows the south-east magnetic linearment corresponding to the Majeon-ri formation and Dark Gray Slate were matched well. From this investigation, we inferred that maximum depth extension which Black Slate member can be separately recognized with respect to adjacent Dark Gray Slate with strong magnetic susceptibility anomaly is about 150m with reference elevation level of 306m. In addition, Majeon-ri formation located south of Black Slate member revels relatively high magnetic susceptibility range but shows high spatial susceptibility fluctation. And, as an intrusive rocks, Jurassic Biotite Granite shows relatively low magnetic susceptibility characteristics. On the contrary, Cretaceous granite distributed in soutthern part of the study area shows the relatively high susceptibility distribution.

Postglacial Vegetation History of the Central Western Region of the Korean Peninsula (한반도 중서부 지역의 후빙기 식생 변천사)

  • Jang, Byeong-O;Yang, Dong-Yoon;Kim, Ju-Yong;Choi, Kee-Ryong
    • Journal of Ecology and Environment
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    • v.29 no.6
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    • pp.573-580
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    • 2006
  • A sediment core from the Cheollipo arboretum ($36^{\circ}$ 47' 57'N, $126^{\circ}$ 09' 04') was studied for pollen analysis in order to reconstruct postglacial vegetational change and environmental changes around the central western region of the Korean Peninsula. The record shows four pollen assemblage zones: Zone CHL-I, Quercus stage (ca. 9,300$\sim$6,200 yr BP): zone CHL-II, Quercus-Pinus stage (ca. 6,200$\sim$4,600 yr BP); zone CHL- III, Pinus-Quercus stage (ca. 4,600$\sim$1,160 yr BP): zone CHL-IV and Pinus stage (ca. 1,160 yr BP-present). During the 9,300$\sim$8,500 yr BP, the early Holocene, researchers have guessed a piece of cool-temperate norihern/altimontane mixed coniferous and deciduous broad-leaved forest. Between 8,500$\sim$4,600 yr BP the Quercus dominated the landscape of study area and the established dates of this typical cool-temperate central/montane deciduous broad-leaved forest vegetation might be ca. 6500 yr BP, and then the Pinus developed around the site at ca. 5,700 yr BP. The abrupt increase of Pinus and NAP (non-arboreal pollen) after ca. 1,100 yr BP indicates the vegetation changes due to human activities. From the dynamics of the Chenopodiaceae pollen indicating salt marsh and freshwater pollen flora such as Typha, Trapa, Nymphoides and so forth, we suggest that the tidal flat was altered into freshwater lake around 6,500 yr BP.

Quaternary Diatom Assemblages from Sediment Core GC 98-06 in the Southern Drake Passage, Antarctica (드레이크 해협 남부 코어퇴적물에서 산출된 제 4기 규조 화석 연구)

  • Lee, Jong-Deock;Yoon, Ho-Il;Yun, Hye-su;Kim, Hyo-Jeong;Bak, Young-Suk
    • Journal of the Korean earth science society
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    • v.23 no.5
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    • pp.442-453
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    • 2002
  • A total of 64 species belonging to 23 genera of diatom fossils are identified from the Core GC 98-06 in the southern Drake Passage, Antarctica. The diatom assemblages are dominated by Actinocyclus actinochilus, Coscinodiscus asteromphalus, Eucampia antarctica, Fragilariopsis kerguelensis, Thalassiosira lentiginosa, T. ritscheri and T. anguste-lineata, which are about 73% of the assemblage. Open water species are more abundant than sea ice species in the diatom assemblages of the core. Fragilariopsis. kerguelensis and Thalassiosira lentiginosa are valuable indicators of the habitats. Especially, F. kerguelensis represent the influence of waters from the Antarctic Circumpolar Current. Sea ice taxa represents the influence of cold waters from Bransfield Strait Water and melt water from the sea-ice at during warm periods. The reworked diatoms such as Denticulopsis dimopha (Miocene) and D. hustedtii (Pliocene) are occurred with Quaternary species (Actinocyclus actinochilus, Fragilariopsis kerguelensis, Thalassiosira lentiginosa, and T. glacilis). The presence of reworked diatoms indicates the transportation of the older diatoms into the Drake passage from the circumference sediments, due to strong bottom current activity of Antarctic circumpolar deep water.

Lead-Zinc-Tin-Silver Mineralization of Tangguanpu Mine, Hunan Province, China: Fluid Inclusion and Sulfur Isotope Studies (중국 호남성 당관포 광산의 연-아연-주석-은 광화작용: 유체포유물 및 황동위원소 연구)

  • 허철호;윤성택;소칠섭
    • Economic and Environmental Geology
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    • v.34 no.2
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    • pp.157-166
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    • 2001
  • Numerous base-metal bearing hydrothermal quartz vein deposits occur in the Hunan province of southern China. The Tangguanpu lead-zinc-tin-silver mine is the major producer among these deposits. Lead-zinc-tin-silver mineralization occurs in a single stage of massive quartz veins which filled fractures in fault zones within Paleozoic metasedimentary rocks. Sphalerite, chalcopyrite, galena, pyrite, arsenopyrite and pyrrhotite are the principal sulphide minerals in the Tangguanpu lead-zinc ores with minor amounts of tin- and antimony-bearing sulphides (stannite, teallite, boulangerite and tetrahedrite). Based on the iron and zinc partitioning between coexisting stannite and sphalerite, the formation temperature for this mineral assemblage range from 300$^{\circ}$ to 330$^{\circ}$C, which relatively agree with the upper part of homogenization temperature of fluid inclusion in quartz (20T-358$^{\circ}$C). Fluid inclusion data show that main lead-zine-tin-silver mineralization occurred from $H_{2}O$-NaCl fluids with relatively low salinities (11.2-7.3 wl.% eg. NaCI) at temperatures between 207$^{\circ}$ and 358$^{\circ}$C. The relationship between homogenization temperature and salinity suggests a history of cooling and dilution followed by initial boiling. Evidence of initial fluid boiling may indicate the fluid trapping pressures of 180 bars. The ${\delta}^{34}S{{\Sigma}S}$ values of -5.0 to 1.1 %, indicate an igneous source of sulfur in the Tangguanpu lead-zinc-tin-silver hydrothermal fluids.

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Geochemical Environments of Copper-bearing Ore Mineralization in the Haman Mineralized Area (함안지역 함 동 광화작용의 지화학적 환경)

  • Choi, Sang-Hoon
    • Economic and Environmental Geology
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    • v.42 no.1
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    • pp.1-8
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    • 2009
  • The Haman mineralized area is located within the Cretaceous Gyeongsang Basin along the southeastern part of the Korean peninsula. Almost all occurrences in the Haman area are representative of copper-bearing polymetallic hydrothermal vein-type mineralization. Within the area are a number of fissure-filling hydrothermal veins which contain tourmaline, quartz and carbonates with Fe-oxide, base-metal sulfide and sulfosalt minerals. The Gunbuk, Jeilgunbuk and Haman mines are each located on such veins. The ore and gangue mineral paragenesis can be divided into three distinct stages: Stage I, tourmaline + quartz + Fe-Cu ore mineralization; Stage II, quartz + sulfides + sulfosalts + carbonates; Stage III, barren calcite. Equilibrium thermodynamic data combined with mineral paragenesis indicate that copper minerals precipitated mainly within a temperature range of $350^{\circ}C$ to $250^{\circ}C$. During early mineralization at $350^{\circ}C$, significant amounts of copper ($10^3$ to $10^2\;ppm$) could be dissolved in weakly acid NaCl solutions. For late mineralization at $250^{\circ}C$, about $10^0$ to $10^{-1}\;ppm$ copper could be dissolved. Equilibrium thermodynamic interpretation indicates that the copper in the Haman-Gunbuk systems could have been transported as a chloride complex and the copper precipitation occurred as a result of cooling accompanied by changes in the geochemical environments ($fs_2$, $fo_2$, pH, etc.) resulting in decrease of solubility of copper chloride complexes.

Analysis of the Epilithic Diatom Community and Comparison of Water Quality in the Kumho River (금호강의 부착규조 군집분석과 수질 변화비교)

  • Choi, Jaesin;Chae, Hyunsik;Kim, Han-soon
    • Korean Journal of Ecology and Environment
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    • v.48 no.2
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    • pp.115-121
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    • 2015
  • In order to assess the water quality of Kumho river using Diatom Assemblage Index to organic water pollution (DAIpo) and to campare from the previous water quality, 5 sites in Kumho river were selected and assessed total of 6 times, every April and September from April 2010 to September 2012. As a result, 116 taxa were found in 2 Order, 3 Suborder, 7 Families, 26 Genera, 103 species and 13 varieties. Saprophilous species of 10 dominant species including Achnanthes minutissima, were increased as going to downstream sites. Correlation coefficients (r) between epilithic diatom communities and physicochemical factors were from - 0.62 to 0.71. Correlation coefficients (r) between TN and diatom indices (DAIpo, TDI) were 0.57 and 0.65, respectively. Therefore, epilithic diatom communities were greatly influenced by TN. The Correlation between DAIpo and TDI measured to be high in correlation coefficient (r=0.62) from the result of correlation analysis. Mean of DAIpo values ranged between 37.24~74.98 and decreased as going to downstream sites. Saprobic level was estimated as ${\beta}$-oligosaprobic at st. 1, ${\alpha}$-oligosaprobic at st. 2 and ${\beta}$-mesosaprobic at st. 3. But st. 4 and 5 which were ${\alpha}$-mesosaprobic and polysaprobic in the previous results, were improved as ${\beta}$-mesosaprobic. The RPId, general assessment of the water quality using DAIpo, was 52.27, which means the water quality of Kumho river was middle level.

Ecosystem Diagnosis and Evaluations Using Various Stream Ecosystem Models (다양한 하천생태모델을 이용한 생태계 진단 및 평가)

  • Kim, Ja-Hyun;Lee, Eui-Haeng;An, Kwang-Guk
    • Korean Journal of Ecology and Environment
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    • v.40 no.3
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    • pp.370-378
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    • 2007
  • The objective of this research was to diagnose integrative ecological health in Bansuk Stream, one of the tributaries of Gap Stream, using the fish assemblage during July 2006${\sim}$April 2006. For this research, we selected six sampling sites and applied some approaches such as the Index of Biological Integrity (IBI), Qualitative Habitat Evaluation Index (QHEI), and necropsy-based Health Assessment Index (HAI). The stream health condition, based on the IBI values, averaged 24 (n= 18, range: $10{\sim}46$), indicating "poor${\sim}$fair" condition according to the criteria of US EPA (1993). Physical habitat condition, based on the QHEI, averaged 116 (n=6, range: $77{\sim}139$), indicating "fair${\sim}$good" condition. Values of IBI were more correlated with 3 metrics of instream cover ($M_1$, r=0.553, p=0.017, n=18), flow/velocity ($M_3$, r=0.627, p=0.005, n=18), and riffes/bends ($M_7$, r=0.631, p=0.005, n=18) than other metrics. Value of HAI in the control was zero (i.e., excellent condition), while the values in the T1 and T2 treatments were 5 (range: 0${\sim}$30) and 50 (range: 40${\sim}$80), respectively. The maximum values of IBI (46) were coincided with zero of HAI. Thus, these approaches seem to be a good tool for a diagnosis and evaluations of stream ecosystem health.

Petrogenesis of Mesozoic granites at Garorim Bay, South Korea: evidence for an exotic block within the southwestern Gyeonggi massif?

  • Kim, Ji In;Choi, Sung Hi;Yi, Keewook
    • Geosciences Journal
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    • v.23 no.1
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    • pp.1-20
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    • 2019
  • We present data from the Mesozoic Keumkang, Palbong, and Baekhwa granites in Garorim Bay, in the southwestern part of the Gyeonggi massif, South Korea. Using major and trace element concentrations, Sr-Nd-Pb isotopic compositions, and sensitive high-resolution ion microprobe (SHRIMP) zircon U-Pb ages, we aim to constrain the petrogenesis of the granites and explain their origin within a broader regional geological context. SHRIMP U-Pb zircon ages of $232.8{\pm}3.2$, $175.9{\pm}1.2$, and $176.8{\pm}9.8$ Ma were obtained from the Keumkang, Palbong and Baekhwa granites, respectively. The Late Triassic Keumkang granites belong to the shoshonite series and show an overall enrichment in large ion lithophile elements (LILE), a depletion in high field strength elements (HFSE) relative to primitive mantle, compared with neighboring elements in the primitive mantle-normalized incompatible trace element diagram with notable high Ba and Sr contents, and negligible Eu anomalies. The Keumkang granites are typified by highly radiogenic Sr and unradiogenic Nd and Pb isotopic compositions: $(^{87}Sr/^{86}Sr)_i=0.70931-0.70959$, $(^{143}Nd/^{144}Nd)_i=0.511472-0.511484$ [$({\varepsilon}_{Nd})_i=-17.0$ to -16.7], and $(^{206}Pb/^{204}Pb)=17.26-17.27$. The Middle Jurassic Palbong and Baekhwa granites belong to the medium- to high-K calc-alkaline series, and show LILE enrichment and HFSE depletion similar to the Keumkang granites, but exhibit significant negative anomalies in Ba, Sr, and Eu. Furthermore, they have elevated Y and Yb contents at any given $SiO_2$ content compared with other Jurassic granitoids from the Gyeonggi massif. The Palbong and Baekhwa granites have slightly less radiogenic Sr and more radiogenic Nd and Pb isotopic compositions [$(^{87}Sr/^{86}Sr)_i=0.70396-0.70908$, $(^{143}Nd/^{144}Nd)_i=0.511622-0.511660$, $({\varepsilon}_{Nd})_i=-15.4$ to -14.7, $(^{206}Pb/^{204}Pb)=17.56-17.76$] relative to the Keumkang granites. The Keumkang granites are considered to have formed in a post-collisional environment following the Permo-Triassic Songrim orogeny that records continent-continent collision between the North and South China blocks, and may have formed by fractional crystallization of metasomatized lithospheric mantle-derived mafic melts. The Palbong and Baekhwa granites may have been produced from a gabbroic assemblage at pressures of less than ~15 kbar, associated with subduction of the paleo-Pacific (Izanagi) plate at the Eurasian continental margin. Elevated ${\varepsilon}_{Nd}(t)$ values in the granitoids from the southwestern part of the Gyeonggi massif relative to those of the central and northern parts, together with the comparatively shallow depth of origin, imply the presence of an exotic block in the Korean lithosphere.

선캠브리아 홍제사 화강암의 진화과정(한국 북동부지역의 원생대의 화성활동과 변성작용)

  • 김정민;조문섭
    • The Journal of the Petrological Society of Korea
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    • v.3 no.1
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    • pp.76-93
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    • 1994
  • The Precambrian Hongjesa granite is lithologically zoned from biotite granite in central part to biotite-muscovite granite towards the margin. The X_{Fe}$ (=Fe/(Fe+Mg)) value and the aluminum saturation index of biotite systematically vary as a function of mineral assemblage, and are positively related with those of bulk rock. This relationship as well as the lithological zoning are attributed to the fractional crystallization of the Hongjesa granitic magma. The trace element data corroborate that biotite-muscovite granite is more fractionated than biotite granite. The evolution of the Hongjesa granite is elucidated by using the AFM liquidus topology, where A=$Al_2O_3-CaO-Na_2O-K_2O$; F=FeO+MnO; and M=MgO. At an early magmatic stage where biotite is the only ferromagnesian mineral to crystallize, the X_{Fe}$ value and the alumina content of granitic magma continuously increase.. Muscovite subsequently crystallizes with biotite along the biotitemuscovite cotectic curve where biotite-muscovite granite forms. Local enrichments in Mn and B further crystallize garnet and tourmaline, respectively. The unique zonal pattern characterized by the occurrence of the evolved biotite-muscovite granite at the margin may be accounted for by the passive stoping during the emplacement of the Hongjesa granite. This emplacement may have occurred in continental collision environment, according to the tectonic discrimination diagram using major element chemistry.

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