• Title/Summary/Keyword: ascorbic acid

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Production of Ascorbic acid-2-phosphate from Ascorbic acid by Pseudomonas sp.. (Pseudomonas sp.에 의한 Ascorbic acid로부터 Ascorbic acid-2-phosphate의 생산)

  • 권기성;이상협;방원기
    • Microbiology and Biotechnology Letters
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    • v.28 no.1
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    • pp.33-38
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    • 2000
  • In order to produce ascorbic acid-2-phosphate from ascorbic acid, bacteria capable of transforming ascorbic acid to ascorbic acid-2-phosphate were isolated from soils and the stock cultures in our laboratory. Among them, a newly isolated bacterium LSH-3 having the best ability of producing ascorbic acid-2-phosphate was selected and partially identified as Pseudomonas sp. The optimum conditions for the production of ascorbic acid-2-phosphate from ascorbic acid and using its resting cells as the source os enzyme were investigated. The results were summarized as follows: The optimum cultivation time and the cell weight for the production of ascorbic acid-2-phosphate was 14 hours and 100g/I(wet weight), respectively. And 0.1%(v/v) Trition X-100 was the most effective surfactant. The optimum concentrations of ascorbic acid and pyrophosphate were 400mM and 500mM, respectively, which led to produce 14.54g/I of ascorbic acid-2-phosphate. The most effective buffer was 50mM sodium acetate. The optimum pH and temperature were 4.5 and $40^{\circ}C$, respectively. Under the above conditions, 17.71 g/I of ascorbic acid-2-phosphate was produced from ascorbic acid after 32 hour-incubation, which corresponded to 17.5% of conversion rate based on ascorbic acid.

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The Comparative Studies for the Anti-stress Effect of Guibitang and Ascorbic Acid on Heating and Swimming Stress (열(熱)Stress 및 유영(游泳)Stress에 대(對)한 귀비탕(歸脾湯)과 Ascorbic Acid의 항(抗)Stress효능(效能) 비교연구(比較硏究))

  • Lee Hwa-Sin;Whang Wei-Wan
    • Journal of Oriental Neuropsychiatry
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    • v.6 no.1
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    • pp.19-39
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    • 1995
  • In order to compare the anti-stress effect of Guibitang with that of ascorbic acid, after these medicines were administered to guinea pigs induced by heating and swimming stress, the changes of the weight of body and organ, and content of plasma catecholamines, serum total cholesterol, free cholesterol, triglyceride, protein, glucose and cortisol were measured. The results were as follows : 1. The weight of the body was increased with statistical significance in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on both heating and swimming stress. 2. The weight of spleen decreased with statistical significance in the groups administered ascorbic acid, Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress, but in case of swimming stress, the weight of spleen decreased with statistical significance in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. The weight of adrenal decreased with statistical significance in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress alone. 3. Plasma norepinephrine content decreased with statistical significance not only in the groups administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid but in the group administered ascorbic acid with Guibitang as compared with in the group administered ascorbic acid on heating stress. In case of swimming stress, norepinephrine decreased with statistical significance in the groups administered ascorbic acid, Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. 4. Plasma dopamine content decreased with statistical significance only in the group administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on both heating and swimming stress. 5. Serum total cholesterol content decreased with statistical significance in the groups administered ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress, but in case of swimming stress, it decreased with statistiscal significance only in the group administered ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. 6. Serum triglyceride content decreased with statistical significance not only in the groups administered ascorbic acid, Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid but in the group administered ascorbic acid with Guibitang as compared with in the group adminstered ascorbic acid on heating stress. In case of swimming stress, triglyceride decrease with statistical significance only in the group administered ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid. 7. Serum glucose content increased with statistical significance in the groups administered Guibitang and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on both heating and swimming stress, particulaly in case of swimming stress, in the group administered ascorbic acid with Guibitang, it increased with statistical significance as compared with in the group administered ascorbic acid. 8. Serum cortisol content decreased with statistical significance only in the group administered ascorbic acid and ascorbic acid with Guibitang as compared with in the group administered non ascorbic acid on heating stress.

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Characteristics of Ascorbic acid Oxidase in Cucumbers (오이의 Ascorbic Acid Oxidase에 관한 연구)

  • Kim, Jung-Won;Park, Eun-Soon;Yoon, Sun
    • Journal of Nutrition and Health
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    • v.18 no.4
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    • pp.312-317
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    • 1985
  • This study was attempted to investigate the occurrence and the characteristics of ascorbic oxidase in cucumbers. Ascorbic acid oxidase was isolated from cucumbers and concentrated using ammonium sulfate precipitation. The results of this study are as follows ; 1) Ascorbic acid oxidase activity was detected in whole cucumber homogenate. 2) Highest amounts of ascorbic acid destroyed after 10 minutes' incubation of ascorbic acid oxidase with its substrate. 3) The optimum pH and temperature of this enzyme were found to be pH 6.5 and $40^{\circ}C$, respectively. 4) Ascorbic acid content in cucumber juice prepared using the cold water $(4^{\circ}C)$ was higher than that made with water at $30^{\circ}C$. 5) When orange juice ( pH 3.4 )was added, ascorbic acid destruction was completely ceased. (The ascorbic acid oxidase was inactivated at pH 3.9) Decreasing the temperature and pH are recommended to achieve maximum stability of ascorbic acid in preparing cucumber juice.

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The Microencapsulated Ascorbic Acid Release in vitro and Its Effect on Iron Bioavailability

  • Lee, Jun-Beum;Ahn, Joung-Jwa;Lee, Jong-Hwi;Kwak, Hae-Soo
    • Archives of Pharmacal Research
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    • v.26 no.10
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    • pp.874-879
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    • 2003
  • The present study was carried out to examine the stability of microencapsulated ascorbic acid in simulated-gastric and intestinal situation in vitro and the effect of microencapsulated ascorbic acid on iron bioavailability. Coating materials used were polyglycerol monostearate (PGMS) and medium-chain triacylglycerol (MCT), and core materials were L-ascorbic acid and ferric ammonium sulfate. When ascorbic acid was microencapsulated by MCT, the release of ascorbic acid was 6.3% at pH 5 and 1.32% at pH 2 in simulated-gastric fluids during 60 min. When ascorbic acid was microencapsulated by PGMS, the more ascorbic acid was released in the range of 9.5 to 16.0%. Comparatively, ascorbic acid release increased significantly as 94.7% and 83.8% coated by MCT and PGMS, respectively, for 60 min incubation in simulated-intestinal fluid. In the subsequent study, we tested whether ascorbic acid enhanced the iron bioavailability or not. In results, serum iron content and transferring saturation increased dramatically when subjects consumed milks containing both encapsulated iron and encapsulated ascorbic acid, compared with those when consumed uncapsulated iron or encapsulated iron without ascorbic acid. Therefore, the present data indicated that microencapsulated ascorbic acid with both PGMS and MCT were effective means for fortifying ascorbic acid into milk and for enhancing the iron bioavailability.

Effect of Liposome on the Stabilization of Ascorbic Acid (Ascorbic Acid 의 안정성에 대한 Liposome 의 효과)

  • Lee, Yu-Weon;Hwang, Yong-Il;Lee, Seung-Cheol
    • Korean Journal of Food Science and Technology
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    • v.31 no.2
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    • pp.280-284
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    • 1999
  • To overcome unstability of ascorbic acid, liposome was used to encapsulate it. Ascorbic acid was encapsulated with 46.8% efficiency inside soybean phosphatidyl choline liposomes by the dehydration-rehydration method. Stability of encapsulated ascorbic acid in liposome was enhanced compared to that in free aqueous solution. For example, most of ascorbic acid in acetate buffer (pH 5.0) was oxidized after 7 days, however, that in liposome was remained as reduced form with 22.8% after 40 days at same conditions. These results mean that encapsulation of ascorbic acid in liposome could provide protection tool for improvement in shelf life.

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The Constraint for Oxidation of Ascorbic Acid (Ascorbic Acid의 산화억제)

  • 이강연;한창규;조춘구
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.25 no.3
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    • pp.67-86
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    • 1999
  • Ascorbic acid which has various physiological benefits as the functional substance is easily oxidized and destroyed by the structural instability. Liposome encapsulated pure ascorbic acid was prepared for the sake of the constraint of oxidation. The influence of cholestrol or $\beta$-sitosterol on the stabilization of liposome was investigated. Butylated hydroxytoluene(BHT), tertiary butylhydroquinone(TBHQ), $\alpha$ -glycosyl rutin and natural concentrated tocopherol were used for constraint of oxidation of ascorbic acid. The presence of cholesterol or $\beta$-sitosterol decreased oxidation of ascorbic acid. That results were thought that cholesterol or $\beta$-sitosterol so increased rigidity of bilayer that the leakage of vitamin C decreased. As a result the oxidation and degradation of vitamin C were constrained. At 0.3w/w% cholesterol content the most stable liposome was formulated. The whole antioxidant that used at the research constrained oxidation of ascorbic acid. The antioxidation for ascorbic acid increased in order of tertiary butylhydroquinone, $\alpha$-glycosyl rutin, butylated hydroxytoluene and natural concentrated tocopherol. But u -glycosyl rutin is preferable to tertiary butylhydroquinone which was the most effective in antioxidation as the antioxidant of ascorbic acid which was utilized in cosmetics and pharmacy.

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The Role of Ascorbic Acid on the Redox Status and the Concentration of Malondialdehyde in Streptozotocin-Induced Diabetic Rats

  • Choi, Hee-Jung;Je, Hyun-Dong;Jeong, Ji-Hoon;Min, Young-Sil;Choi, Tae-Sik;Park, Joon-Hong;Shin, Chang-Yell;Sohn, Uy-Dong
    • Archives of Pharmacal Research
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    • v.26 no.3
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    • pp.237-243
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    • 2003
  • We investigated the role of ascorbic acid on the redox status in streptozotocin-induced diabetic rats. In the plasma of diabetic rats, the ratio of reduced/total ascorbic acid was significantly decreased as compared with normal control. Ascorbic acid supplementation increased the reduced and total ascorbic acid contents as compared with diabetic control. In the rutintreatment group, reduced and total contents of ascorbic acid were significantly decreased, however, the ratio of reduced/total contents of ascorbic acid had no difference as compared with diabetic rats. In the insulin-treatment group, this ratio is not significantly different as compared with diabetic control. However, in the insulin plus ascorbic acid treatment group, reduced form and the ratio of reduced/total ascorbic acid were significantly increased as compared with diabetic control. In addition, we measured the contents of malondialdehyde (MDA) in the plasma of diabetic rats. The contents of MDA was increased as compared with normal control, however, in insulin-treatment group, the contents of MDA was decreased as compared with diabetic rats. Ascorbic acid had no effects on the increases of MDA in diabetic rats. In conclusion, plasma ascorbic acid level and its reduced/total ratio reflects the status of the oxidative stress in the diabetic rats. Supplement of ascorbic acid did not correct the ratio of the reduced/total ascorbic acid. However, supplement of insulin and ascorbic acid corrected the ratio of reduced/total ascorbic acid.

Studies on the Synthesis of L-Ascorbic acid-3-Aminopropane Phosphoric Acid Diester and its Applications (L-Ascorbic acid-3-aminopropane phosphoric acid diester의 합성과 응용에 관한 연구)

  • 이옥섭;이기화
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.23 no.2
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    • pp.97-117
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    • 1997
  • L-Ascorbic acid 및 그 유도체는 항산화 작용과 미백작용 및 섬유아세포의 생장 촉진과 콜라젠 생합성의 증가시키는 효과가 있으므로 화장품에서 오래 전부터 사용되어 왔다. 본 연구에서는 생체에 대한 안전성과 안정성이 우수한 L-Ascorbic acid 유도체를 개발하기 위하여 인지질과 유사하게 L-Ascorbic acid 에 3-Aminopropane phosphoric acid를 결합하여 L-Ascorbic acid-3-aminopropane phosphoric acid diester를 합성하였다. ASA-APPA 는 2-Chlorotetrahydro-2H-1, 3, 2-oxazaphosphorine P-oxide 와 5, 6-isopropylidene L-Ascorbic acid 를 반응 시킨 후 산 사수분해 반응을 통하여 얻을 수 있었다. ASA-APPA는 수용액에서 안정성이 우수하였으며, 독성실험에서 무독성 물질이며, 인체 첩포 실험에서도 무자극 물질임을 확인하였다. 그리고, ASA-APPA 는 L-Ascorbic acid 와 3-APPA 의 혼합물과 거의 유사한 섬유아세포의 증식효과를 나타내었으며, melanoma 에 대한 멜라닌 생성 억제 실험에서 L-Ascorbic acid phosphate magnesium salt 와 유사한 효과를 보였다. 따라서 ASA-APPA 는 멜라닌 생성 억제 효과와 섬유아세포의 증식 효과를 갖는 새로운 화장품 원료로서 사용이 가능할 것으로 생각된다.

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Stability of Ascorbic Acid in the Solutions of Sugars and Amino Acids (당질과 아미노산이 Ascorbic Acid 의 안정도에 미치는 영향)

  • Hwang, Hee-Za
    • Journal of Nutrition and Health
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    • v.15 no.1
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    • pp.22-29
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    • 1982
  • As sugar and amino acid were added to the ascorbic acid solution the content of ascorbic acid was quantitatively determined by 2, 4-dinitrophenyl hydrazine method. The residual ascorbic acid was shown to increase slightly when sorbose, rhamnose or mannose was added to the ascorbic acid solution whereas residual ascorbic acid was shown to decrease in time to the addition of other sugars. The effects of amino acid to the ascorbic acid solution were found that monoamino-mono, or dicarboxylic acids and aromatic amino acids increased the residual ascorbic acidity whereas diamino-monocarboxylic acids and sulfur containing amino acids decreased the residual ascorbic acidity.

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Microencapsulated Ascorbic Acid for Milk Fortification

  • Lee, J.B.;Ahn, J.;Kwak, H.S.
    • Archives of Pharmacal Research
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    • v.26 no.7
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    • pp.575-580
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    • 2003
  • The present study was designed to develop a microencapsulated L-ascorbic acid and iron that could be used to fortify milk and to determine the sensory properties of milk fortified with microencapuslation. Coating material was medium-chain triacylglycerol (MCT), and selected core material was ferric ammonium sulfate and L-ascorbic acid. The highest efficiency of microencapsulation was 95.0% in the ratio of 15:1 as coating to core material. Ascorbic acid release was increased sharply up to 5 d storage as 6.5%. TBA value was the lowest when both capsulated iron and ascorbic acid were added during 12 d storage, compared with other treatments. In sensory analysis, most aspects were not significantly different between control and capsulated ascorbic acid fortified milk at 5 d storage. The present study indicated that the use of microencapsulated ascorbic acid with MCT is effective for fortifying milk. In addition, these results suggest that acceptable milk products can be prepared with microencapsulated ascorbic acid and iron.