• Title/Summary/Keyword: artificial structure

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Improvement of Functional Assessment for Riverine Wetlands using HGM Approach (HGM 적용을 통한 하도습지의 기능평가 제고 방안 연구)

  • Yeum, Junghun;Kim, Taesung
    • Journal of Wetlands Research
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    • v.18 no.4
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    • pp.378-385
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    • 2016
  • This study aims to suggest the framework of functional assessment on lotic area based on HGM(Hydrogeomorphic) approach targeting Wetland Protected Areas which are in the type of river channel, and to set up the fundamental data as a reference wetland. A total of 10 factors in terms of hydrology, biogeochemistry, plant habitat and animal habitat was analyzed based on the original approach of HGM and each Functional Capacity Index(FCI) of those factors was calculated. As the result of the modified FCI analysis, Damyang riverine wetland which is with artificial river bank had high values in the variables of area ratio of actual vegetation in the foreland, the number of plant per area and the area ratio of Salix spp., and those values were highly reflected on the factors of Nutrient Cycling(947,668.00), Species Richness and Maintain Characteristic Plant Communites(6.39) and Maintain Spatial Structure of Habitat(11.00). The Hanbando wetland which is keeping the natural bank had higher values in the variables of structural scale and species diversity, and the those values were highly reflected on the factors of Energy Dissipation(17,805.16), Subsurface Storage of Water(0.54), Removal of Imported Elements and Compounds(103,052.73), Maintain Characteristic Detrital Biomass(2.31), Maintenance of Interspersion and Connectivity (6.50), Species Diversity of Benthic macro-invertebrates(1.60) and Species Diversity of Vertebrate & Species Number of Other Animals(2.52/ 151.50), compared to the Damyang Riverine Wetland.

Facilitation of tolaasin-induced hemolysis by phospholipids composed of medium-chain fatty acids (중간크기 탄소사슬의 지방산으로 이루어진 인지질에 의한 tolaasin의 용혈활성 촉진)

  • Yun, Yeong-Bae;Kim, Min-Hee;Kim, Young-Kee
    • Journal of Applied Biological Chemistry
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    • v.59 no.3
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    • pp.221-225
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    • 2016
  • Tolaasin is a pore-forming peptide toxin produced by Pseudomonas tolaasii and causes a brown blotch disease by disrupting membrane structures of cultivated mushrooms. The mechanism and characteristics of tolaasin pore formation are not known in detail; however, tolaasin pores have been demonstrated in the artificial lipid bilayer. Since the tolaasin pore appeared less frequently and unstable in lipid bilayer, a mismatch between the length of tolaasin pore and the thickness of lipid membrane had been suggested. Therefore, tolaasin-induced hemolyses were measured by the additions of phospholipids composed of various fatty acids with different carbon numbers. When phosphatidylethanolamines made with two decanoic acids (C10:0, 1,2-didecanoyl-sn-glycero-3-phosphoethanolamine; DDPE), myristic acids (C14:0, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine), and stearic acids (C18:0, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine) were added to the buffer containing RBCs and tolaasin peptides, DDPE facilitated the tolaasin-induced hemolysis while the other two phospholipids showed no effects. At various concentrations of DDPE, the tolaasin-induced hemolysis was stimulated as a dose-dependent manner. The phospholipids composed of mediumchain fatty acids stabilize the tolaasin pore probably by binding between the pore structure and membrane phospholipids and making the membrane thickness thinner around the pore. These results showed that tolaasin molecules make more stable pores in the membrane made with phospholipids composed of medium length fatty acids, suggesting that the length of tolaasin pore is a little shorter than the thickness of RBC membrane.

Impedance Spectroscopy Analysis on the LaAlO3/SrxCa1-xTiO3/SrTiO3 Hetero-Oxide Interface System

  • Park, Da-Hee;Kwon, Kyoung-Woo;Park, Chan-Rok;Choi, Yoo-Jin;Bae, Seung-Muk;Baek, Senug-Hyub;Kim, Jin-Sang;Hwang, Jin-Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.188.2-188.2
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    • 2015
  • The presence of the conduction interface in epitaxial $LaAlO_3/SrTiO_3$ thin films has opened up challenging applications which can be expanded to next-generation nano-electronics. The metallic conduction path is associated with two adjacent insulating materials. Such device structure is applicable to frequency-dependent impedance spectroscopy. Impedance spectroscopy allows for simultaneous measurement of resistivity and dielectric constants, systematic identification of the underlying electrical origins, and the estimation of the electrical homogeneity in the corresponding electrical origins. Such unique capability is combined with the intentional control on the interface composition composed of $SrTiO_3$ and $CaTiO_3$, which can be denoted by $SrxCa1-_xTiO_3$. The underlying $Sr_xCa1-_xTiO_3$ interface was deposited using pulsed-laser deposition, followed by the epitaxial $LaAlO_3$ thin films. The platinum electrodes were constructed using metal shadow masks, in order to accommodate 2-point electrode configuration. Impedance spectroscopy was performed as the function of the relative ratio of Sr to Ca. The respective impedance spectra were analyzed in terms of the equivalent circuit models. Furthermore, the impedance spectra were monitored as a function of temperature. The ac-based characterization in the 2-dimensional conduction path supplements the dc-based electrical analysis. The artificial manipulation of the interface composition will be discussed towards the electrical application of 2-dimensional materials to the semiconductor devices in replacement for the current Si-based devices.

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Effect of Freeze-Thaw Cycles after Cracking Damage on the Flexural Behavior of Reinforced Concrete Beams (균열손상 후 동결융해를 경험한 철근콘크리트 보의 휨거동)

  • Kim, Sun-Woo;Choi, Ki-Bong;Yun, Hyun-Do
    • Journal of the Korea Concrete Institute
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    • v.22 no.3
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    • pp.399-407
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    • 2010
  • The flexural behaviors of two types of beam members exposed to freeze-thaw cycles were evaluated. This study aims to examine the effect of freeze-thaw cycles on the behavior characteristics of reinforced concrete (RC) beams. For the purpose, a part of the beam specimens were damaged until yielding of tension reinforcement was reached, before they were exposed to 150 and 300 cycles of freeze-thaw. Cyclic tests, as well as monotonic tests, were conducted to evaluate the stiffness degradation characteristics when same cycle is repeated. The material tests showed that relative dynamic modulus of concrete exposed to 300 cycles of freeze-thaw moderately decreased to 86.8% of normal concrete, indicating that concrete used in this study has good durability against freeze and thaw damage. The results of monotonic tests showed reduction of flexural strength, ductility and stiffness of the beam specimens exposed to freeze-thaw cycles compared with those of the control speciments. In particular, BDF13 specimens, which had been subjected to artificial cracking damage, did not showed enough flexural strength to satisfy nominal moment required by current concrete structure design code. In the monotonic tests results, BF75 specimens exposed to freeze-thaw cycles showed 10% or more cyclic stiffness degradation. Therefore, it was thought that deformation of concrete in compression have to be considered in design process of members under cyclic load, such as seismic device.

An Experimental Study on Scour at V-shaped Riffle (V형 여울에서 발생하는 세굴에 관한 실험 연구)

  • Yu, Dae-Young;Park, Jung-Hwan;Woo, Hyo-Seop
    • Journal of Korea Water Resources Association
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    • v.36 no.3 s.134
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    • pp.507-520
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    • 2003
  • A V-shaped riffle is an artificial hydraulic structure haying two wings from the streamside with a narrow opening in between. It is usually made of crushed stones or large boulders. It limits channel width and accelerates the flow through the constricted section causing a local scour just downstream. The V-shaped riffle provides with a unique aquatic habitat by forming a pool and sandbars around the pool edge, increasing local morphologic, hydraulic and sedimentological diversity. This study investigates experimentally the scour characteristics of the V-shaped riffle in the sandbed stream and proposes a predictive equation for the scour. Total 45 cases of experiments were conducted to examine the effect of hydraulic factors and configuration of V-shaped riffle on the geometry of scour holes. From the comparison of the experimental results of this study with the predictive equation of spur dike by Breusers and Raudkivi(1991), it is found that their predictive equation of spur dike underestimates the maximum scour depth downstream of the V-shaped riffle. h new predictive equation for the maximum scour depth was developed using the non-dimensional hydraulic and geometrical variables. The parameters used in the proposed equations were determined using the experimental data. The analysis reveals that the scour depth is dependent dominantly on the Froude number at the opening of the V-shaped riffle, while the angle of riffle and the opening width also affect the scour depth. The proposed equation for the scour of V-shaped riffle well agrees with the experimental data. It can be used for estimating the scour of the V-shaped riffle in sandbed streams.

Integrated Simulation System of GIS and ANN for Land Price Appraisal (GIS 기반 지가산정 및 시뮬레이션 시스템)

  • Moon, Tae-Heon
    • Journal of the Korean Association of Geographic Information Studies
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    • v.3 no.2
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    • pp.1-10
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    • 2000
  • The purpose of this study is to develope a parcel-based automatic simulation system of land price through the integration of urban mathematical model and GIS. The appraisal process of public land price by the local government is simple but is a great time-consuming task. Moreover, it doesn't provide any statistical analysis and spatial presentation tools. So, it is difficult for planners or administrative officials to analyze the variation of land price with spatial idea. From these, a system is developed combining two sub-systems, they are ANN(Artificial Neural Network) for the calculation of land price and GIS for visual presentation. Using Matlab application, ANN model was designed having 3-layer structure and was trained with the sample data taken from Chinju city. With the trained network, the impact of 'road', 'parks', 'height control district' and 'beauty district' on land price in 9 regions(dong) are simulated. The results of the simulation were visualized with ArcView GIS. The automatic simulation system operated through the DDE(Dynamic Data Exchange) conversation between two applications. ArcView was set as client and Matlab as server. Scripting in ArcView and customizing a window of ArcView, this system can execute the whole process of simulation by just clicking a button with mouse. As a conclusion, this system was proved to be an effective and easily controllable planning support system for the land price simulation.

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A Study on the Tissue Response and Bone Formation after Augmentation Using Proplast and Porous Polyethylene in Rabbit Mandible (Proplast와 Porous Polyethylene을 이용(利用)한 가토(家兎) 하악골(下顎骨) 증대술후(增大術後) 조직반응(組織反應) 및 골형성(骨形成)에 관(關)한 연구(硏究))

  • Kim, Geon-Jung;Ryu, Sun-Youl
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.11 no.1
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    • pp.101-116
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    • 1989
  • Proplast and Porous Polyethylene which have porous structures as low-modulus polymers have been recently used in maxillofacial plastic and reconstructive surgery. The purpose of this study was to compare the response of adajacent tissue, new bone formation and stability after augmentation by differen methods of subperiosteal graft using proplast and purous polythylene in rabbit mandible. The augmentation procedure was carried out by dividing into two groups, A and B. A group consisted of subperiosteal graft on the cortex, and the other B group was made up only graft following artificial decortication in the mandibular body of rabbit. The experimental animals were sacrificed on the 1st, 2nd, 4th and 8th week after grafting for macroscopic and light microscopic examination. The samples extracted at the 6th postgrafting week were also used for biometric testing and scanning electron microscopic examination. The results obtained from this study were as follows : 1. Macroscopically, infection of graft site, deformation and migration of graft material were not observed in all experimental groups. 2. B group showed more rapid and increased bone formation and the greater stability than A group, and tissue response was similar to each other. 3. In the tissue response, macrophages and cellular infiltrations were observed in Proplast group, but few in PHDPE group. 4. In bone formation of A group, Proplast group showed no bone formation until the 8th week, but PHDPE group showed small quantity of osteoid tissue from the 2nd week and appositional bone growth with new bone formation at the 8th week. 5. In bone formation of B group, both Proplast and PHDPE group showed bone formation, but PHDPE group showed more rapid and larger bone formation. 6. In pattern of bone formation, Proplast group mainly showed appositional bone growth pattern connected with graft site. On the other hand, PHDPE group showed mixed pattern of new bone formation in the pore connective tissue with appositional bone growth from graff site. 7. The maximum mean values of shear stress were serially $111.3gf/mm^{2}$ in PHDPE of B group, $84.8gf/mm^{2}$ in PHDPE of A group, $32.9gf/mm^{2}$ in Proplast B group, and $15.7gf/mm^{2}$ in Proplast of A group. From above results, It was suggested that the capacity of bone formation and stability between bone and graft material were dependent on the pore size and structure of graft material itself, the state of graft site and tissue response.

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Laboratory Study of the Shear Characteristics of Fault Gouges Around Mt. Gumjung, Busan (부산 금정산일대에 분포하는 단층비지의 전단특성에 관한 실험적 고찰)

  • Woo, Ik
    • The Journal of Engineering Geology
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    • v.22 no.1
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    • pp.113-121
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    • 2012
  • The mechanical characteristics of a fault gouge from near Mt. Kumjung in Kumjung-Gu, Busan, were estimated from laboratory tests on different joint models. Fault gouge samples and joint samples in biotite granite were obtained from boreholes in the study area that had penetrated small faults associated with the Dongnae and Yangsan faults. XRD and SEM analyses revealed that for the fault gouge consists of several clay minerals with tabular structure (kaolinite, montmorillonite, illite, sericite), which could cause the considerable reduction of shear strength when wet. The shear strength of the fault gouge was obtained from direct shear tests of the fault gouge itself and from direct shear tests of several natural/artificial joint surfaces coated with fault gouge. The results indicate that the reduction of shear strength is more abrupt for the joint surfaces coated with fault gouge compared with uncoated joint surfaces, and that the friction angle of the fault gouge between joint surfaces is much lower than the internal friction angle of the fault gouge itself. Fault gouges in contact with rock, therefore, could have a stronger negative effect on the stability of structures in rock masses than the fault gouge itself.

Prediction and analysis of acute fish toxicity of pesticides to the rainbow trout using 2D-QSAR (2D-QSAR방법을 이용한 농약류의 무지개 송어 급성 어독성 분석 및 예측)

  • Song, In-Sik;Cha, Ji-Young;Lee, Sung-Kwang
    • Analytical Science and Technology
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    • v.24 no.6
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    • pp.544-555
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    • 2011
  • The acute toxicity in the rainbow trout (Oncorhynchus mykiss) was analyzed and predicted using quantitative structure-activity relationships (QSAR). The aquatic toxicity, 96h $LC_{50}$ (median lethal concentration) of 275 organic pesticides, was obtained from EU-funded project DEMETRA. Prediction models were derived from 558 2D molecular descriptors, calculated in PreADMET. The linear (multiple linear regression) and nonlinear (support vector machine and artificial neural network) learning methods were optimized by taking into account the statistical parameters between the experimental and predicted p$LC_{50}$. After preprocessing, population based forward selection were used to select the best subsets of descriptors in the learning methods including 5-fold cross-validation procedure. The support vector machine model was used as the best model ($R^2_{CV}$=0.677, RMSECV=0.887, MSECV=0.674) and also correctly classified 87% for the training set according to EU regulation criteria. The MLR model could describe the structural characteristics of toxic chemicals and interaction with lipid membrane of fish. All the developed models were validated by 5 fold cross-validation and Y-scrambling test.

Genomic Diversity of Helicobacter pylori

  • Lee, Woo-Kon;Choi, Sang-Haeng;Park, Seong-Gyu;Choi, Yeo-Jeong;Choe, Mi-Young;Park, Jeong-Won;Jung, Sun-Ae;Byun, Eun-Young;Song, Jae-Young;Jung, Tae-Sung;Lee, Byung-Sang;Baik, Seung-Chul;Cho, Myung-Je
    • The Journal of the Korean Society for Microbiology
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    • v.34 no.6
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    • pp.519-532
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    • 1999
  • Helicobacter pylori is a causative agent of type B gastritis and plays a central role in the pathogenesis of gastroduodenal ulcer and gastric cancer. To elucidate the host-parasite relationship of the H. pylori infection on the basis of molecular biology, we tried to evaluate the genomic diversity of H. pylori. An ordered overlapping bacterial artificial chromosome (BAC) library of a Korean isolate, H. pylori 51 was constructed to set up a genomic map. A circular physical map was constructed by aligning ApaI, NotI and SfiI-digested chromosomal DNA. When the physical map of H. pylori 51 was compared to that of unrelated strain, H. pylori 26695, completely different restriction patterns were shown. Fifteen known genes were mapped on the chromosome of H. pylori 51 and the genetic map was compared with those of strain 26695 and J99, of which the entire genomic sequences were reported. There were some variability in the gene location as well as gene order among three strains. For further analysis on the genomic diversity of H. pylori, when comparing the genomic structure of 150 H. pylori Korean isolates with one another, genomic macrodiversity of H. pylori was characterized by several features: whether or not susceptible to restriction digestion of the chromsome, variation in chromosomal restriction fingerprint and/or high frequency of gene rearrangement. We also examined the extent of allelic variation in nucleotide or deduced amino acid sequences at the individual gene level. fucT, cagA and vacA were confirmed to carry regions of high variation in nucleotide sequence among strains. The plasticity zone and strain-specific genes of H. pylori 51 were analyzed and compared with the former two genomic sequences. It should be noted that the H. pylori 51-specific sequences were dispersed on the chromosome, not congregated in the plasticity zone unlike J99- or 26695-specific genes, suggesting the high frequency of gene rearrangement in H. pylori genome. The genome of H. pylori 51 shows differences in the overall genomic organization, gene order, and even in the nucleotide sequences among the H. pylori strains, which are far greater than the differences reported on the genomic diversity of H. pylori.

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