• Journal of Mushroom
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• v.5 no.2
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• pp.81-85
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• 2007

This study was carried out to investigate the conditions of liquid culture and fruiting body formation of Cordyceps militaris using grain mediacontained the brown rice and black purple rice. Liquid mycelial growth and density of Cordyceps militaris were the highest in the medium of malt extract 0.7% and pH 5.0~5.5. The optimal condition of formation the fruiting bodies were shown to be temperature of $17^{\circ}C$, light intensity of 600 lux, $C0_2$ of 500ppm and humidity intensity of 95% produced highest amount of fruit bodies.

• Journal of Mushroom
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• v.13 no.3
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• pp.151-156
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• 2015

Cordyceps militaris is being studied and cultivated as a medicinal mushroom having many valuable biological and pharmaceutical activities. In the breeding of new C. militaris mushroom, single ascospores were isolated and examined their mycelial growth, mycelial density, and production of stroma and perithecia. Among them selected isolates were crossed and hybrids were produced showing high quality fruiting bodies on artificial media. Mycelial growth rate of new strain 'Dowonhongcho' was higher than that of better on 'Yedang 3' on SDAY at $10-25^{\circ}C$. The stromata of new strain were club-shaped and bright orange-red. Its height was 6.1 cm and the cordycepin content was 0.34% on average. The new strain showed 9% higher yield than 'Yedang 3' with producing firmer fruit bodies. The optimum temperature for mycelial growth was $22{\sim}25^{\circ}C$ and the optimum temperature for stroma development was $18{\sim}22^{\circ}C$. Fruiting bodies were began to produce 45 days later after inoculation. This new cultivar may serve as a valuable one for artificial cultivation and industrial-scale production of C. militaris.

• Journal of Mushroom
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• v.15 no.3
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• pp.104-110
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• 2017

In order to determine the possibility of artificial cultivation of Ganoderma lucidum, a study was carried out to optimize the medium composition used for bed culture. Sawdust spawn of medium composition for optimal growth was found to be the combination of 75% oak-sawdust, 10% cotton-seed meal, and 15% beet pulp. The optimal conditions for the formation of fruiting bodies were shown to be a temperature of $28-30^{\circ}C$, light intensity of 1,000-1,500 lux, and $CO_2$ concentration of 1%.

• The Korean Journal of Mycology
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• v.46 no.1
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• pp.34-42
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• 2018

Tricholoma matsutake (Pine mushroom) is expensive, and its artificial cultivation has been tried in several countries. Until date, the only successful cultivation of artificial pine mushroom in pine forests uses matsutake-infected pine trees. The National Institute of Forest Science in Korea has been restudying this method since 2000. Success in fruit production and reproduction was achieved in 2010 and 2017, respectively, in the same locale. The successes proved that pine mushrooms could be cultivated artificially in the field using matsutake-infected pine trees. The fruiting of pine mushroom in October 2010 occurred 6 years, 6 months after the transplantation of matsutake-infected pine trees. Five pine mushrooms reoccurred in September 2017, 13 years, 5 months and 15 years, 5 months after the transplantation of the respective matsutake-infected pine trees. The distance between the matsutake-infected pine tree and the pine mushrooms was 12 cm at 6.6 years, 90~115 cm at 13.5 years, and 95 cm at 15.5 years. Fruiting bodies occurred 13~16 days after the underground temperature declined to below $19^{\circ}C$. In conclusion, the use of matsutake-infected pine trees remains the only way to artificially cultivate pine mushrooms. More knowledge of the environmental factors affecting matsutake fruiting would be beneficial.

• Journal of Practical Agriculture & Fisheries Research
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• v.5 no.1
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• pp.8-12
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• 2003

The optimal log conditions of cultivation are as follows. 1) Log-length, 100~120cm 2) Hole-depth, 1~1.5cm 3) It takes 55~67 days for primodia induction is possible to harvest fruiting bodies for 6~7 months. Meanwhile, the total of 10 species pest including Trichoderma sp. occurred during the cultivation.

• The Plant Pathology Journal
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• v.40 no.1
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• pp.83-97
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• 2024

Fusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strain was capable of producing perithecia predominantly in the central region of the fungal culture where aerial hyphae naturally collapsed. To unravel the intricate relationship between natural aerial hyphae collapse and sexual development in this fungus, we focused on 699 genes differentially expressed during aerial hyphae collapse, with 26 selected for further analysis. Targeted gene deletion and quantitative real-time PCR analyses elucidated the functions of specific genes during natural aerial hyphae collapse and perithecium formation. Furthermore, comparative gene expression analyses between natural collapse and artificial removal conditions reveal distinct temporal profiles, with the latter inducing a more rapid and pronounced response, particularly in MAT gene expression. Notably, FGSG_09210 and FGSG_09896 play crucial roles in sexual development and aerial hyphae growth, respectively. Taken together, it is plausible that if aerial hyphae collapse occurs on plant debris, it may serve as a physical cue for inducing perithecium formation in crop fields, representing a survival strategy for F. graminearum during winter. Insights into the molecular mechanisms underlying aerial hyphae collapse provides offer potential strategies for disease control against FHB caused by F. graminearum.

• Korean Journal of Pharmacognosy
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• v.24 no.2
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• pp.116-123
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• 1993

The genus Ganoderma is typical wood-rotting fungi and its fruiting body has been used as an important herb in oriental medicine. Recent research discovered antitumor components from Ganoderma lncidum. Various Ganoderma species are being cultivated in Korea. However, taxonomic system of the genus Ganoderma has been based mainly on the macromorphology of fruiting bodies and the ultrastructural characteristics of basidiospores. Since there are similar characteristics in Ganoderma mycelia grown on the same artificial media, it is suggested that the compatibility of the fungi by di-mon mating be used as an aid to determine the identity of species in addition to the conventional characterization. In this study, we examined physiological and genetical properties such as growth temperature, pH, compatibility and enzyme or protein patterns of laccase, esterase and cellular proteins of G. lucidum RZ, G. tsugae and Ganoderma species cultivated in Korea by electrophoresis for characterization of the isolates. We found that compatibility test and isozyme patterns of laccase and esterase of the mycelia could be used for the differentiation of the isolates. These results showed that Ganoderma species cultivated in Korea is genetically similar to G. lucidum but physiologically closer to G. tsugae than to G. lucidum.

• The Korean Journal of Mycology
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• v.25 no.4 s.83
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• pp.311-319
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• 1997

These studies were carried out to develop an artificial cultivation method. The diameter and thickness of pileus ranged $1.5{\sim}7.0\;cm$ and $0.8{\sim}3.0\;cm$, respectively. The diameters of stipe were $1.2{\sim}2.5\;cm$ and the lengthes were $4.5{\sim}9.0\;cm$. The spore fingerprint was white. The sizes were spore $10.8{\sim}12.2{\times}4.35{\sim}5.65\;{\mu}m$, basidia $50.0{\sim}59.2{\times}7.4{\sim}7.8\;{\mu}m$, nalsistidia $21.75{\sim}28.7{\times}4.8{\sim}6.1\;{\mu}m$, pileus hymenium cell $50.6{\sim}66.0{\times}4.4{\sim}6.7\;{\mu}m$, and stipe hymenium cell $28.6{\sim}33.0{\times}5.5{\sim}6.6\;{\mu}m$. The thirty percent mixture of rice and wheat bran into sawdust gives the high density of mycelia and the good development of fruiting structure. The optimum water contents of sawdust substrates were $60{\sim}65%$ in which condition the mycelium grows well and gives high density. In PP bottle cultivation, the first fruiting period was $6{\sim}8$ days earlier in nonscratching samples than scratching ones, but the quantity of fruiting body was higher in scratching samples than nonscratching ones. In the case of PP bag cultivation, the first fruiting was 10 days faster, and the quantity of fruiting bodies was 30% higher in samples with 30% wheat bran than those with rice bran. The fleshiness of stipe was $2{\sim}3$ times harder than that of pileus.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.31-35
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• 2008

To elucidate optimum light conditions for artificial cultivation of Grifola frondosa, the effects of light quality (blue, green, white) and light intensity (200, 500, 800, 1200 lux) on primordium formation, morphological properties and yield of fruiting bodies of G. frondosa using bag cultivation method were tested. Among three light sources, white light source ($400{\sim}620\;nm$) had a higher mushroom yield (242 g/bag) and a shorter cultivation period (52 days) than those of the others. In particular, blue light source ($400{\sim}560\;nm$) induced the morphology of wide and deep colored pilei in G. frondosa fruiting body. The experimental results on the appropriate light intensity indicated that 500 lux light was the most effective on mushroom production, whereas primordium formation was effective at 200 lux.

• Journal of Sericultural and Entomological Science
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• v.41 no.1
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• pp.36-40
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• 1999

The optimal condition for the production of the artificial fruiting bodies of Paecilomyces japonica in bulk was determined from the observation of the conidial, hyphal, and cultural condition and the medium. The colony was grown 32 mm diameter in 14 days on potato dextrose agar(PDA). Conidia was irregularly long oval-shaped and measured 4.07${\times}$1.56 ${\mu}$m in average. The hyaline hyphae formed transparent bundles which branched out. The fruiting body on the insect surface was measured 30 to 50 mm in length and formed up to 55 branches. Potato dextrose(PD) both was the most effective for the growth of Paecilomyces japonica among the liquid type media. About 3.1 mg mrcelia in dry weight were produced in 50 ml/PD broth. The best condition for the production of conidia under which condition 4.3${\times}$10$^8$ conidia/ml were harvested was pH 5.0 in acidity and 20$^{\circ}C$ following preculture at 24$^{\circ}C$ in temperature for 7 days.