• Korean Journal of Clinical Pharmacy
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• v.15 no.2
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• pp.118-126
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• 2005

In our study, osteoporosis was induced by ovariectomized in female Sprague-Daweley (SD) rats, and the prevention and treatment efficacy of the climacteric disease the ostmenopausal type I pattern was examined by using the experimental substance soybeans and arowroot. No clinical signs and mortality after continuous oral administration of experimental substances for 14 weeks were not observed, however, the weight of experimental animals underwent ovariectomized was increased significantly in comparison with control group (p<0.01). The atrophy of the uterus due to T3 group was 23.64% after 7 weeks, 24.44% after 14 weeks (in comparison with N.C. group), and it was statistically significantly increased. In regard to the change of blood cells, it was observed that platelets were statistically significantly reduced in the ovariectomized group, and administered orally experimental substances continuously for 7 weeks, in all groups administered experimental substances, it was found that platelets had the tendency to be increased more than N. C. group. In regard to He change of blood biochemistry, removed the ovary, the concentration of ALP showed the tendency to be increased than control group, and particularly in T3 group, it was increased significantly. In regard to the concentration of cholesterol, in comparison with negative control group, it was reduced 68% at 7 weeks and 35% at 14 weeks. After ovariectomized, the amount of estrogen was found to be reduced by 21.37% in comparison with control group, it showed the tendency to be increased by 4.49% in T1 group and 7.62% in T2 group, the concentration of estrogen in each group showed the tendency to be increased than negative control group, and in T3 group, it was increased to 100.46% and 117.65% in T4 group, and it was increased more than control group. Based on the above experimental results, in the experimental animals female rats, because of the hormonal imbalance induced by ovariectomized, a large mount of fat is accumulated in the body and due to it osteoporosis, obesity, hypertension, hyperlipidemia, fat of the liver, arteriosclerosis, diabetes, and other metabolic diseases were developed. Hence, when the experimental substance Extraction of ferment arrowroot was orally administered continuously for 14 weeks, it was thought that a certain proportion of the hormonal balance was maintained that functioned as a substance interfering the accumulation of fat, and it was considered to be of help in the treatment of not only osteoporosis Type I, but also for the prevention and treatment of various endocrinal diseases.

• Korean Journal of Medicinal Crop Science
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• v.10 no.2
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• pp.93-99
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• 2002

This study was designed to investigate the pharmacological effects of glasswort. SD rats drunk experimental water (added to glass wort extracts) for 4 weeks. Weight gain resulted in reduced by administration of glasswort extracts compared with control group(p < 0.05). Total protein and albumin contents in serum of SD rats for 4 weeks did not show significantly difference by administration of glasswort extracts compared with control group. GOT was $126.7{\sim}134.1\;U/L$ and GPT was $41.1{\sim}46.7\;U/L$ by administration of glasswort extracts in SD rats for 4 weeks. Total and LDL cholesterol contents in serum of SD rats significantly decreased by administration of glasswort extracts compared with control group(p < 0.05) HDl cholesterol contents in serum of SD rats was significantly increased by administration of glasswort extracts compared with control group(p < 0.05). Total lipid and triglyceride contents in serum of SD rats was significantly decreased by administration of glasswort extracts compared with control group (p < 0.05). The overall results suggest that the administration of glasswort extracts can not only prevent a disease of arteriosclerosis, hyperlipidemia, fatty liver but also inhibit of weight gain.

• The Journal of Korean Medicine
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• v.18 no.1
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• pp.72-81
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• 1997

The causes of stroke are presumed hypertension, atherosclerosis,. cardiac disease, diabetes mellitus and old age and risk factors of stroke are suggested hypertension, hyperlipidemia. obesity, smoking and drinking etc. Especilly, hypertension is one of the most important cause and risk factor of stroke, therfore without therapy hypertension leads to stroke. The frequence of hypertension is significantly higher in hemorrhage patients of intracranial hemorrhage and subarachnoid hemorrhage. Antihypertensive therapy has an impact not only on the primary prevention of stroke but also on stroke recurrence and the declining of stroke motality has been attributed to the widespread availability and use of antihypertensive therapy. The goals of antihypertensive therapy decrease the complications and motalitv of cardiovascular system and prevent the promoting arteriosclerosis. In this study, we observed the blood pressure change of cb-hemorrhagic patients with hypertension who were hospitalized from 1996. 3. 1 to 1997. 2 .26 in Wonkwang Oriental Hospital. These patients had no antihypertensive therapy and were supplied herb med(Jaum sikpoongtang, Chungrijagamtang, Gojinumja) in medication. Our results suggested as follows. 1. Systolic and diastolic average BP at admission is $150.71{\pm}15.61mmHg$ and $95.00{\pm}8.8mmHg$ and this is hypertension state defining WHO 2. During one week in admission, the blood pressure demostrated a marked declination by SBP 8.97mmHg and DBP 6.22mmHg. 3. During two week in admission, the declination of SBP was significant in paired t-test(p<0.05) but, the declination of DBP was non-significant in paired t-test. 4. The blood pressure during third and fourth weeks gradually declined but, non-significant in test. According to the above results, we suggested that the BP declination Was affected by $Jamyang{\cdot}Jaum$ prescriptions in acute stage of cerebral hemorrhage.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.12
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• pp.1827-1834
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• 2014

Colon cancer is the third highest cause of death in Korea. Known dietary causes of colon cancer include a diet rich in fat and red meat as well as inadequate intake of dietary fiber, fruits, and vegetables. Therefore, recent research has focused on the anticancer effects of natural products. Opuntia humifusa is a type of prickly pear that is known to contain biologically active compounds that can be used in the treatment of diabetes mellitus, arteriosclerosis, and hyperglycemia. The aim of this study was to determine whether or not O. humifusa extract affects proliferation, cell death, and DNA fragmentation in human carcinoma HT-29 cells. O. humifusa is rich in carbohydrates, minerals (Mg, K, and Ca), and total phenolics. HT-29 cells were treated with extracts of O. humifusa at concentrations of 0, 0.25, 0.5, 1, and 2 mg/mL for 24 or 48 hours. O. humifusa extracts inhibited HT-29 cell growth in a dose-dependent manner. Hoechst 33342/PI double staining and Comet assay were performed to observe changes in nuclei of cancer cells undergoing cell death. The results of both tests showed that O. humifusa extract induced cell shrinkage, DNA fragmentation, and chromatin condensation dose-dependently in HT-29 cells. The results of this study suggest that O. humifusa extract inhibits the growth of HT-29 via induction of DNA fragmentation and chromatin condensation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.4
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• pp.531-537
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• 2011

The effects of dietary supplementation of pacific saury on anti-hyperlipidemic activities were investigated using an animal test study in which normal rats were fed four different parts of saury, such as the whole body, meat, internal organs, or a mixture of head, caudal fin, and bone. Serum total lipid and triglyceride levels were significantly (p<0.05) reduced in rats fed saury meat at a dose of 200 mg/kg of body weight compared to hyperlipidemic control rats. There were also significant decreases in serum total cholesterol and LDL-cholesterol levels in the rats fed saury meat at 200 mg/kg of body weight. In addition, the atheroscrelosis index and superoxide dismutase in blood lipids were significantly (p<0.05) lowered in rats fed saury meat at 200 mg/kg of body weight compared to the control rats. In conclusion, our results indicate that saury meat contains unknown physiologically active components as than compared to other parts of saury, and has potential for use in the prevention of hyperlipidemic arteriosclerosis.

• Journal of Sericultural and Entomological Science
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• v.46 no.1
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• pp.1-5
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• 2004

Rutin has many beneficial effects on human health. It was established that rutin antagonizes the increase of capillary fragility associated with hemorrhagic disease, reduces high blood pressure, decreases the permeability of the vessels, and has an antiedema effect, reduces the risk of arteriosclerosis, and shows antioxidant activity. Rutin analysis with mulberry fruits was carried out by high-performance liquid chromatography (HPLC) to reveal the possibility of source of functional food. As a result, mean content of fifty accessions was 0.14±0.050% DW. Among the tested fifty accessions, 'Sabangso' was showed the highest rutin in 0.29%DW, whereas 'Simseol' was the lowest content in 0.05% DW. Also, we researched the change of rutin content according to harvested date. The rutin contents of earlier harvested groups were higher than later harvested groups. Simultaneously, fruity characteristics as well as rutin content were researched and analyzed to select the functional mulberry varieties for the production of fruit. From the six accessions which were contained high rutin content, we selected three suitable varieties such as 'Ficus', 'Kangsun', and 'Palcheongsipyung'.

• Journal of Sasang Constitutional Medicine
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• v.10 no.2
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• pp.485-511
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• 1998

In orther to find the characteristics of each constitution, the examine of Kyung Hee medical center was diagnosed constitution, and resulted body measures and diagnosis. That was considered and the results are as follows 1. The obese patients is that there are the best many people(91.0%) to Taeumin. The frequency of Taeumin group is more than Soyangin group and Soeumin group. The degree of obesity grade is the highest in Taeumin. 2. The systolic blood & diastolic blood pressure of obese patients did not show any significant differences. Only, the obesity patients is the higher than nomal examine, without regard to Sasang constitution. 3. The hypertensive frequency of obese patients is that Taeumin group is more than Soyangin group and Soeumin group. there is not show any significant differences. specially, the hypertension of Soyangin group is caused by an ascending of diastolic pressure. 4. Glucose value of obese patients did not show any significant differences. generally, the frequency of Taeumin group is more than Soyangin group and Soeumin group. The diabetes of obese patients is the highest in Taeumin. 5. The serum lipids of obese patients did not show any significant differences. Total cholesterol, Triglyceride value of Taeumin group is more than Soyangin group and Soeumin group. HDL-cholesterol of obese patients is that Taeumin group is more than Soyangin group and Soeumin group. 6. The frequency of hyperlipidemia is the highest in Taeumin. 7. GOT, GPT of obese patients did not show any significant differences, but GGT is higher in Taeumin than Soyangin & Soeumin group. 8. Uric acid of obesity patients did not show any significant differences between Sasang constitution, but the value of obese patients is higher than nomal examine. The abnomality of Uric acid is higher in Taeumin than the other constitutions. The frequency of abnomality is the highest in Taeumin females. 9. Arteriosclerosis frequency of obese patients did not show any significant differences, the frequency of Sasang constitution is higher in Taeumin than Soyangin & Soeumin group. 10. Hemoglobin of obese patients did not show any significant differences, but Hematocrit is higher in Soyangin than Taeumin & Soeumin group. Anemia of obese patients did not show any significant differences. The frequency of anemia is higher in the females than the males. 11. BUN, creatinine of obese patients did not show any significant differences.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.513-521
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• 2005

A bacterium producing five fibrinolytic isozymes was isolated from black bean chung kuk. The bacterium was identified as Bacillus subtilis BB-1 by 16s rDNA sequence homology search. A gene out of five fibrinolytic genes in the Bacillus subtilis BB-1 was cloned by shot-gun method. A Cla I DNA fragment of B. subtilis BB-1 chromosome was cloned in to pBluescript II SK(-) and showed the fibrinolytic activity to bacterial cells. The Cla I DNA fragment was sequenced and the sequences did not show homology with gene for protease or fibrinolytic enzyme genes in other organisms. The Cla I DNA fragment was reduced to 2,142 bp by activity-guided PCR cloning method. The optimum pH and temperature of the enzyme were 5.0 and $35^{\circ}C$, respectively. Substrate specificity of the fibrinolytic enzyme was detected in skim milk, casein, gelatin and blood agar plates. The activity of the enzyme was not detected with these substrates. Taken together, this enzyme is a new fibrinolytic enzyme and may be used to prevent thrombosis and arteriosclerosis.

• The Journal of the Society of Korean Medicine Diagnostics
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• v.13 no.2
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• pp.88-116
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• 2009

Background : EBM (the evidence based medicine) is the direction of the development for oriental medicine. The pulse meter-analyzer is the important part to standardize the pulse diagnosis. When we set up the direction of the study on the pulse meter-analyzer, the awereness about the pulse meter and analyzer of the clinical oriental medical doctors as consumers is very important. Objectives : In order to prepare for the mutual study and the export strategy on the pulse meter-analyzer of Korea and China, the attitude of the study and the grasp of the awereness about the pulse meter-analyzer of the Chinese medical doctors are very important. Methods : We developed the several items as the important factor of the development of the pulse meter and analyzer. They were translated by chinese medical doctor. The investigation for demend was conducted during 3 months in Guangzhou City, Guangdong Province, China. The results of the investigation was done the statistical method of frequency analysis, Chi-squared test, correspondence analysis. Results : The most important differentiation of symptom is the symptom of JANG-BU(臟腑). The method of the utility in the pulse diagnosis is Chon-Gu (寸口) pulse diagnosis and the research on Chon-Kwan-Cheok(寸關尺) is the most important measurement factor. And the typical the old pulse is the little-fine weak pulse. The pulse meter-analyzer is most suitable to the diagnosis of the hypertension and the arteriosclerosis. Conclusion : The development of the pulse analyzer including the requests of Korean and Chinese medical doctors is very important. Specially the researches on the influence factors of the traditional diagnosis and the environment of the measurement are important for developing the pulse analyze.

• Journal of Pharmacopuncture
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• v.9 no.2
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• pp.17-37
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• 2006

Objectives : To observe the changes in the serum proteins after intravenous injection of cultivated wild ginseng pharmacopuncture. Methods : Blood was collected before and after the administration of cultivated wild ginseng pharmacopuncture and only the serum was taken. Then differences in the spots on the scanned image after carrying out 2-Dimensional electrophoresis were located and conducted mass analysis and protein identification. Results : Following results were obtained from the comparative analysis of serum proteins before and after the administration of cultivated wild ginseng pharmacopuncture. 1. 28 spots were identified before and after the administration. 2. In confirming manifestation degree, spots with more than two-times increase were 204, 1302, 2205, 3105, 7104, 8006, spots with more than one-time increase were 1101, 1505, 2013, 2403, 3009, 3010, 4002, 4009, 6704, 8101, and spots with decrease were 205, 801, 803, 3205, 5202, 6105, 6106, 7103, 9001, 9003. 3. After conducting protein identification, proteins 205, 804, 1302, 4009, 6105, 6106 are unidentified yet, and 1l01 is unnamed protein. Protein 204 is identified as complement receptor CR2-C3d, 801 as YAPl protein, 803 as antitrypsin polymer, 1505 as PRO0684, 2013 and 3010 as proapolipoprotein, 2205 as USP48, 2403 as vitamin D binding protein, 3009 as complement component 4A preprotein, 3105 as immunoglobulin lambda chain, 3205 as transthyretin, 4002 as Ras-related protein Ral-A, 4204 as beta actin, 5202 and 7104 as apolipoprotein Ll, 6704 as alpha 2 macroglobulin precursor, 7103 as complement component 3 precursor, 8006 as testis-specific protein Y, 8101 as transferrin, 9001 as (Alpha-Oxy, Beta-(Cl12g)deoxy) T-State Human Hemoglobin, and 9003 as human hemoglobin. 4. Immune protein CR2-C3d(204), which acts against microbes and pathogenic organisms, was increased by more than two-times after the administration of pharmacopuncture. 5. Antitrypsin(803), which is secreted with inflammatory response in the lungs, was reduced after the administration of pharmacopuncture. 6. Proapolipoprotein(2013, 3010) and apolipoprotein(7104), key components of the HDL-cholesterol which plays an important role in preventing arteriosclerosis, were increased after the administration of pharmacopuncture. 7. Vitamin D binding protein(DBP, 2403), protecting the lung at the time of inflammatory response, was increased after the administration of pharmacopuncture. 8. Transthyretin(TTR, 3205), which is the main protein causing familial amyloid polyneuropathy(FAP), was decreased after the administration of pharmacopuncture. 9. Ras-related protein Ral-A(4002) that controls phospholipid metabolism, cytoskeletal formation, and membrane traffic, was increased after the administration of pharmacopuncture. 10. Testis-specific protein Y(8006), which takes part in determination of the gender, was increased by more than two-times after the administration of pharmacopuncture. 11. Transferrin(8101), which balances the iron level in the body, was increased after the administration of pharmacopuncture. Conclusion : Above results support the notion that intravenous injection of cultivated wild ginseng pharmacopuncture induce changes in serum proteins and this research can be a pioneer work in finding biomarkers.