• Journal of Microbiology and Biotechnology
• /
• v.17 no.5
• /
• pp.812-821
• /
• 2007

The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.

• Journal of Microbiology and Biotechnology
• /
• v.14 no.3
• /
• pp.593-598
• /
• 2004

The arsenical resistance (ars) operon was cloned from a 67-kilobase pair (kb) plasmid, which was previously shown to be responsible for arsenic salts resistance in K. oxytoca D12. When plasmid pAE48, carrying the ars operon, was transformed into E. coli, transformed cells displayed enhanced survival in the presence of 4 mM arsenite, 50 mM arsenate, or 0.4 mM antimonite. The nucleotide sequence of the 5.6-kb fragment encoding arsenical resistance revealed five open reading frames (ORFs), which were predicted to encode polypeptides of 12.8 (arsR), 13.4 (arsD), 62.6 (arsA), 45 (arsB), and 16.7 (arse) kilodaltons (kDa). Each ORF was preceded by a ribosome binding site. A putative promoter-like sequence was identified upstream of arsR, and a possible termination site was found downstream of arsC. When the deduced amino acid sequences of the K. oxytoca Dl2 Ars proteins were compared with the amino acid sequences of the E. coli R773 Ars proteins, a significant amino acid similarity was observed (87.9％ for ArsR, 89.2％ for ArsD, 83.2％ for ArsA, 92.6％ for ArsB, and 91.3％ for ArsC), suggesting an evolutionary relationship of the ars genes of E. coli plasmid R773 and K. oxytoca Dl2.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.15 no.12
• /
• pp.2679-2686
• /
• 2011

Whereas ARS(Automatic Response System)s are a typical communication system very widely used, it has a predominating reputation that it is of great inconvenience. This irony does not stem from technological deficiency, but rather from the usability problem. Under the current situation that there is little research and results in terms of ARS usability, we introduce an ARS simulator designed to help ARS usability tests more effectively. As a system for ARS usability researchers, it provides an environment for the mock task design and users' performance of the task. Further, it saves the mock task history and analyzes users' action patterns to some extent.

• Journal of Life Science
• /
• v.13 no.2
• /
• pp.191-196
• /
• 2003

The ars gene of the Neurospora crassa encodes arylsulfatase and is expressed under sulfur limitation. An ars-1 promoter(Pars) translationally-fused to a lacZ gene was transformed into the N. crassa RLM 35-35, a his-3 inl strain and integrated into the his-S locus by a single crossover homologous recombination. $\beta$-galactosidase specific activity was measured from mycelia grown in sulfur-limited Vogel's medium. Enzyme activity reached its maximum at 14 hour after the shift to derepressing condition. When activity from homokaryon generated by microconidiation was measured, it was 17% a higher than that from heterokaryon.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.21 no.2
• /
• pp.72-80
• /
• 2013

This study is to develop and evaluate an AFS and an ARS controllers to enhance lateral stability of a vehicle. A sliding mode control (SMC) and a fuzzy logic control (FLC) methods are applied to calculate the desired additional steering angle of AFS equipped vehicle or desired rear steer angle of ARS equipped vehicle. To validate AFS and ARS systems, an eight degree of freedom, nonlinear vehicle model and an ABS controllers are also used. Several road conditions are used to test the performances. The results showed that the yaw rate of the AFS and the ARS vehicle followed the reference yaw rate very well within the adhesion limit. However, the AFS improves the lateral stability near the limit compared with the ARS. Because the SMC and the FLC show similar vehicle responses, performance discrimination is small. On split-${\mu}$ road, the AFS and the ARS vehicle had enhanced the lateral stability.

• Journal of Veterinary Clinics
• /
• v.35 no.5
• /
• pp.200-205
• /
• 2018

This retrospective study is designed to compare the clinical results of tibial tuberosity transposition (TTT) and anti-rotation suture (ARS) treatments for medial patellar luxation (MPL). Medical records of 133 dogs were reviewed that had undergone surgical correction of MPL between January 2013 and May 2017. MPL correction was performed on 182 stifles, with TTT and ARS being performed on 101 stifles and 81 stifles. The common dog breeds receiving surgical treatment for MPL were Maltese, Pomeranian, Chihuahua and Poodle. Mean age of dogs with MPL was 32.6 months, and their mean body weight was 4.26 kg. Seventy dogs (52.6%) were male and 63 (47.4%) were female. Of the 182 stifles with MPL, grade II, III and IV were 18.7%, 72.0% and 9.3%. Total complications after TTT and ARS were recorded in 16.8% and 29.6%. Major complications after TTT and ARS were recorded in 5.9% and 12.3%, minor complications after TTT and ARS were recorded in 10.9% and 17.3%. The risk of complication and reluxation rate after TTT were significantly lower than that for ARS (p < 0.05). However, the rate of reluxation among dogs treated by ARS (1.2%) for grade II MPL was significantly lower than that for dogs treated by ARS (8.6%) for grade III MPL (p < 0.05). The TTT group had a shorter recovery period after surgical intervention than that in the ARS group (p < 0.001). In conclusion, TTT had a significantly lower incidence of complication and a shorter recovery period than ARS. However, ARS for grade II MPL appears to be a good surgical option for reducing the rate of reluxation after surgery. These results of this study could be used to provide therapeutic guidelines for surgical MPL correction in small-breed dogs.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.15 no.2
• /
• pp.462-470
• /
• 2011

ARS (Automatic Response Systems) based on VUI (Voice User Interface) and TTI (Touch Tone Interface) are one of the most widely used communication systems. Despite common usages, however, inconvenience of ARS is continually pointed out. This may stem from lack of human-centered studies aside from technological development. In this paper, we provide guidelines for designing ARS by analyzing hindrance factors of usability of ARS menu structure. We had selected two call-centers using ARS, and carried out an experimental study where subjects performed the task of "returning books." After that, they completed questionnaires and interviews. We identified four problems: the complex menu structure, lack of representativeness on the menu name, users' awareness of location, and a difficulty to move among menus. And we partially discussed the ways of avoiding the problems.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2006.04a
• /
• pp.292-293
• /
• 2006

• Proceedings of The Korean Society of Agricultural and Forest Meteorology Conference
• /
• 2019.08a
• /
• pp.130-143
• /
• 2019

• Journal of Fisheries and Marine Sciences Education
• /
• v.28 no.3
• /
• pp.630-644
• /
• 2016

We conducted a comprehensive review on artificial reefs (ARs) including seaweed reefs for marine afforestation in relation to their development and research from 1972 to 2016, and then systematically analyzed their current a state-of-the-art and practice. From the review, the followings conclusions are made. First, the objectives of AR projects in the Southeast Asia can be classified into three, i.e. protection and increase of fishery resources, local community's profits, and ecological tourism. Second, fish gathering effects by ARs can be determined in terms of wake region or wake length that tends to increase with the K-C (Keulegan-Carpenter) number. Third, ARs are desirable to deploy across a direction of the main flow but it is essential to keep the deployment interval two to four times the length of a single reef. Fourth, stability of ARs depends on how to evaluate drag coefficient, and Morison formula turns out to be practical. Fifth, local scours of ARs are likely to occur due to a downward flow around the center of the front surface. Finally, it is natural for ARs to positively contribute to the marine ecosystem but it is imperative to develop an evaluation method for the effects of ARs on the marine ecosystem.