• 한국수산과학회지
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• 제54권4호
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• pp.377-387
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• 2021

Fecal contamination levels of discharge water from inland pollution sources were investigated in Iwon-myeon (Taean-gun), South Korea. Gram-negative bacteria were isolated during the investigation and the antimicrobial resistance patterns of the isolates were examined to estimate their impact on the coastal environment. The ranges of total coliform and fecal coliform of 12 samples from four major inland pollution sources were 79-490,000 MPN/100 mL and 2.0-490,000 MPN/100 mL, respectively, with the highest level of fecal contamination at Station No. 3. A total of 137 strains (14 genus) were isolated, of which 86 strains (62.8%) were Enterobacteriaceae. The identified isolates were as follows: Pseudomonas spp. (35 strains), Klebsiella spp. (20 strains), Serratia spp. (20 strains), and Escherichia spp. (19 strains). The isolated Gram-negative bacteria showed the highest antimicrobial resistance to ampicillin (81.8%), followed by amoxicillin/clavulanic acid (64.2%), ceftiofur (61.3%), and cefoxitin (59.1%). Antimicrobials in which less than 10% of isolates showed antimicrobial resistance were ciprofloxacin (3.6%) and gentamicin (2.2%). Resistance to one or more antimicrobials was observed in 121 strains (88.3%) and 84 strains (61.3%) showed a tendency for multiple antimicrobial resistance.

• 한국수산과학회지
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• 제56권6호
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• pp.798-809
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• 2023

In this study, we evaluated the impacts of land-based pollution sources on seawater and shellfish in the Jindongman area after 20.5 mm and 90.6 mm rainfall events. We analyzed sanitary indicator microorganisms used in survey management, such as total coliform, fecal coliform, Escherichia coli, and male-specific coliphage in Waste water treatment plant (WWTP), major inland pollution source,s seawater, and shellfish for 4 days after rainfall events. Our results showed that the range of coliform group and fecal coliform was 1.8-49 and <1.8-4.5 MPN (most probable number)/100 mL, respectively, after rainfall events in WWTP discharge water. Furthermore, the radius of the calculated impacted area of major inland pollution sources ranged from 5 to 798 m after 20.5 mm of rainfall and 30 to 1,031 m after 90.6 mm of rainfall. The fecal coliform of seawater at 30 stations in the shellfish growing area and areas adjacent to four stations was <1.8-130 and from <1.8-79 MPN/100 mL, respectively. The E. coli level of shellfish at 7 stations in the shellfish growing area was <18-220 MPN/100 g.

• 한국환경보건학회지
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• 제23권2호
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• pp.89-97
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• 1997

Most of urban streams in Korea have been changed channel forms and suffered from direct inflow of domestic sewage, etc. Therefore, maintenance of structure and function of those ecosystem are hard. The present study was carried out to examine the life survival maintenance ability of the stream by community analysis of aquatic animals in typical urban stream (Jungrang stream) in Seoul. The aquatic animals were composed of 31 species, 18 families, 8 orders, 5 classes in 4 phyla. Seasonal species number showed big fluctuation between 8 species in Winter and 24 species in Autumn. Major dominant species in Jungrang stream were Tubificidae sp.1, Chironomidae sp.1, Chironomidae sp.2 and Physa acuta, and above endurance species for water pollution occupied very high dominance indices. But, Cercion hieroglyphicum, Ischnura asiatica, Rantra chinensis, Herochares striatus, Agabus japonicus in benthic macroinvertebrates of a few individuals are appeared. Also, fry of Carassius auratus and Silurus asotus in fish are occurred. Therefore, we can be inferred on posibility of growth and spawning of above species in the stream. Jungrang stream has a small quantity of natural riffle areas, ponds and watergrass areas by channel form of water course. Aquatic animals in Jungrang stream has been suffered by reduction of self-purification reaction ability and have mass production of attached algae on the stream bed. For analysis of fluctuation of life survival maintenance ability in Jungrang stream, long-term survey is needed.

• 한국농촌건축학회논문집
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• 제2권1호
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• pp.89-100
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• 2000

Recently, according to the increase of population and rapid growth of industry, the amount of effluent pollutant has been rising in natural water. In these pollutant, nutrients such as nitrogen and phosphor are told that these evolve the odor, color and eutrophication in rural housing sewage and lake regulary. Many researches have been carried out to remove these nutrients from effluents and will have to be studied more deeply. Especially, because of the sewage of rural housing and livestock, environmental pollution raises serious problem in a rural community. This method is developed to solve the problem environmentally friendly. Using the natural energy(wasteheat, earthheat, solar engery) and the growth properties of aquatic plants are most efficient method to absorb the nutrients and denitrification and phosphor uptake.

• 생태와환경
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• 제39권4호통권118호
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• pp.445-449
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• 2006

Phenolic compounds are manufacturing by-products commonly found in industrial wastewater. The toxicity of high level phenolic compounds in wastewater threatens not only the aquatic organisms, but also many components of the adjacent ecosystem. One of the major light harvesting pigments in cyanobacteria is phycocyanin which can be rapidly and specifically degraded by external stimuli such as nutritional depletion or environmental stress. We employed the cyanobacterium Anabaena sp. PCC 7120 as an indicator organism in estimating the pollution level by phenolic compounds. The phycocyanin content of the cyanobacterium decreased without significantly altering the total chlorophyll as the phenol concentration in a medium increased. We examined the phenol contamination level using the correlation of the phycocyanin content and the phenol concentration. Our results indicated that no significant pollution by phenolic compounds was found in several waterbodies in the vicinity of Daegu, South Korea.

• 한국해양바이오학회지
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• 제5권4호
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• pp.46-50
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• 2011

Phytoplankton forms the base of sea ecosystems. Various environmental factors and anthropogenic pollution, primarily, affect the concentration and photosynthetic activity algal cells, and the changes in the phytoplankton photosynthesis influence other elements of aquatic ecosystems. The increase in anthropogenic pollution markedly damages natural aquatic ecosystems, particularly, in the coastal zones, where an intense blooming of microalgae occurs, including the release of highly dangerous ecotoxic substances of various chemical natures (red tides). In this study, we tried to apply as a parameter for the algal blooming prediction in the ocean from fluorescence values in the taken samples around Busan coastal area. F0 value was almost constant but Fv/Fm value showed the irregular pattern. We presume that these results are due to the changes of the ocean environment and climate. To predict or give early warning the algal blooming, we need to investigate the specific area or fixed area through real-time monitoring. Especially, algal blooming prediction or warning can be achieved via continuously monitoring and interpretation of fluorescence changes.

• 한국수산과학회지
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• 제45권2호
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• pp.154-160
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• 2012

This study the effects of Cu exposure on biochemical factors in the hemolymph and hepatopancreas of the abalone $Haliotis$ $discus$ $hannai$. Abalone were exposed to 0, 5, 10, 20 and 40 ${\mu}g/L$ Cu for 4 weeks. The calcium concentrations in hemolymph were decreased significantly on exposure to 20 and 40 ${\mu}g/L$ Cu after 2 weeks. The aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) activities in abalone hemolymph were markedly elevated after exposure to 40 ${\mu}g/L$ Cu for 4 weeks. The hepatopancreas superoxide dismutase (SOD) and catalase (CAT) activities were also significantly increased by exposure to 20 ${\mu}g/L$ Cu for 4 weeks. These biochemical factors may represent a convenient method of monitoring heavy metal pollution in coastal areas. From these results, we conclude that a high copper concentration (40 ${\mu}g/L$) in water may curtail hemolymph homeostasis and anti-oxidative reactions in abalone.

• 한국양식학회지
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• 제18권3호
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• pp.173-179
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• 2005

Superoxide dismutase (SOD), an antioxidant enzyme catalyzing the first step for scavenging the reactive oxygen species is important as an early warning indicator to address various biological stresses. For this reason, the monitoring the expressed pattern of SOD gene in fish organs is one of important biomarkers to assess the aquatic pollution caused by many toxic chemicals. Based on the Northern blot hybridization, semi-quantitative and/or realtime RT-PCRs, the alteration of SOD gene transcripts in shark liver was examined during the experimental acute exposures to cadmium. The expression of SOD at mRNA level was up-regulated both by injection (0, 0.5, 1 or 2 mg $CdCl_2/kg$ body weight for 48 hours) and by immersion (0 or $5{\mu}M$ Cd for 0, 1, 4 and 7 days) treatments of cadmium. The transcriptional stimulation of shark SOD gene by cadmium exposure was dependent upon doses and durations: there was a trend toward more increase in higher dose and longer durations of exposure. The hepatic SOD mRNA levels showed also a general agreement with the tissue cadmium concentrations accumulated in immersion exposure. This result may provide useful strategy to develop a fine molecular biomarker at mRNA level for detecting aquatic pollution caused by toxic metals.

• Fisheries and Aquatic Sciences
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• 제19권4호
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• pp.17.1-17.9
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• 2016

The seasonal variation of fecal coliforms (FCs) and physicochemical factors was determined in seawaters of the Hansan-Geojeman area, including a designated area for oyster, and in inland pollution sources of its drainage basin. The mean daily loads of FCs in inland pollution sources ranged from $1.2{\times}10^9$ to $3.1{\times}10^{11}$ most probable number (MPN)/day; however, the pollutants could not be reached at the designated area. FC concentrations of seawaters were closely related to season, rainfall, and inland contaminants, however, within the regulation limit of various countries for shellfish. The highest concentrations for chemical oxygen demand (COD) and $chlorophyll-{\alpha}$ in seawaters were shown in the surface layer during August with high rainfall, whereas the lowest for dissolved oxygen (DO) in the bottom layer of the same month. Therefore, it indicates that the concentrations of FC, COD, DO, and $chlorophyll-{\alpha}$ of seawaters were closely related to season and rainfall.

• 한국수산과학회지
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• 제46권4호
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• pp.437-444
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• 2013

Eight fecal sterols were analyzed in surface sediments collected from shellfish farming areas in Yeoja Bay and Gangjin Bay, Korea, to evaluate sewage-derived fecal pollution. The concentrations of coprostanol, a good marker of sewage-derived organic contamination, in sediments were in the range of 10-530 ng/g-dry in Yeoja Bay, and 10-190 ng/g-dry in Gangjin Bay. Coprostanol levels were markedly higher in the inner bay than in the outer bay. These levels were lower than those reported in urbanized bays in Korea, however, they were comparable to levels in other shellfish farming areas including Gamak Bay. A multivariate analysis of the ratios of other sterols suggested that the sterols originated from sewage and plankton/benthos. Sewage was the dominant source at stations located close to the river mouth and wastewater treatment plant (WWTP) outfalls, and plankton/benthos was the primary source in the outer bay. These results suggest that management of point sources, e.g., WWTP as well as non-point sources, e.g., riverine inputs is important for improving the water quality in Yeoja Bay and Gangjin Bay.