• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.150-156
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• 2000

Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. The 30 fatty acids which were identified or characterized are classified. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Cluster 3 and 4-a strains were containing Q-8, these strains are accommodated in the Proteobacteria gamma and beta subdivision. The chemotaxonomic profiles of the cluster 4-a strains showed good agreement with those of the genus Burkholderia. Cluster 3 was characterized by the presence of branched-chain fatty acids, iso-C15:0, iso-C17:1, and iso-C17:0 as the major components. These chemotaxonomy suggested the close relationship of the isolates with Xathomonas/Sterotrophomonas group. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Sterotrophomonas, but were not included in these genera. These strains were even further away from core Xanthomonas, and clearly were seen to branch outside the cluster formed by the Sterotrophomonas maltophilia. MH256 and MA828 16S rDNA sequence was different enough to put new genus on a separate branch. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium.

• Korean Journal of Ecology and Environment
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• v.43 no.4
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• pp.459-470
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• 2010

In this study, we analyzed fish tolerance guilds in mainstems and tributaries of 65 streams and rivers arid their relations to water quality using dataset sampled from April to November, 2009. For the study, water quality parameters including biochemical oxygen demand (BOD), electric conductivity (EC), total nitrogen (TN), total phosphorus (TP), ammonia nitrogen ($NH_3$-N), nitrate nitrogen ($NO_3$-N) and phosphate phosphorus ($PO_4$-P) were analyzed in the laboratory and also tolerance ranges in 3 category fishes of sensitive, intermediate, and tolerant species with high abundance were analyzed. According to fish guild analysis, tolerant species was 58% of the total community and the proportion of omnivore species was 63% of the total, indicating a degradation of habitats and water quality. Water quality was shown typical longitudinal gradients from the headwater to the down-river; TN and TP increased toward the down-rivers except for the big point-source area and ionic contents, based on, electric conductivity showed same pattern. Tolerance guild analysis of 9 major species with high abundance indicated that sensitive groups had narrower tolerance range in the water quality than the groups of intermediate and tolerant species. In contrast, tolerant groups including Zacco platypus, Carassius auratus, and Opsarichthys uncirostris amurensis had wider tolerance ranges than the groups of sensitive and intermediate species. Thus, each group was evidently segregated from the tolerance levels. Principal Component Analysis (PCA) employed for the relations of water quality to fish species in each groups suggests that water quality had highest eigenvalues with fish species in the 1st axis of the PCA and nitrogen (TN, $NH_3$-N, $NO_3$-N) and phosphorus (TP) were key components differentiating three groups of sensitive, intermediate and tolerance guilds.

• Journal of The Geomorphological Association of Korea
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• v.24 no.4
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• pp.57-73
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• 2017

The formation of the lava dam of the paleo lake blocked the entrance to the Chatancheon River on the Jeongok lava plateau and it suddenly transformed the terrestrial ecosystem into the aquatic one by the overflow. The spiders in the lava dam adapted in the wetland and evolved into water spiders that could survive by forming bubble houses. Since then, the lava dam was connected to the present Hantangang River due to the dissection and the lake became a terrestrial environment, a small area of marsh composed of primarily clay soil layer. Change in water level of the habitat and thus the extension of the terrestrial area made the species a endangered now. This study carried out frequency of occurrence, degree of wetness and plant habitats of the vascular plant in the water spider habitat. As a result of this study, total 180taxa are of 55 (30.6%) wetland plant groups and of 113 (62.8%) upland plant groups except facultative plant groups. Among the wetland plant groups, the Isachne globosa community occupied the largest area, where the water spiders were most observed. The result of this study, the classification and the types of vascular plant species, would provide useful information for the sustaining healthy wetland ecosystem and the restoration of the habitat for the water spiders.

• Journal of Wetlands Research
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• v.17 no.1
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• pp.53-61
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• 2015

Habitat creation for endangered species Menyanthes trifoliata L. using planting module represents a habitat type such as the rhizome grows horizontally to open water at the margin of the lake. The objectives of this mesocosm experiment are habitat creation with easy construction and low management effort, and to investigate the potential of providing a habitat for aquatic macroinvertebrates. Planting modules had three different substrates of bed soil, perlite and K-SOIL (artificial lightweight soil using bottom ash). These modules were established in two different size of the tub($1170{\times}2250{\times}300mm^3$, $900{\times}1360{\times}190mm^3$). According to the monitoring results, number of leaves and coverage of M. trifoliata showed significant difference with substrate and tub size. The number of leaves showed similar growth responses in bed soil (mean 22.979) and K-SOIL (mean 28.042) substrates but growth was poor in perlite substrate (mean 1.667). The number of leaves in the large tub was more than small tub (p=0.015). Similar responses were obtained with the coverage, the length of rhizome and the number of rhizome in M. trifoliata. A total of 21 taxa of aquatic macroinvertebrates including 1,145 individuals was found in the mesocosm. The Shannon diversity index and colonization index in the mesocosm were similar to the previous studies. These results suggest that the experimental mesocosm could provide sufficient habitats for aquatic macroinvertebrates. If planting modules use bed soil or K-SOIL by planting substrate, establish that taking into account open water surfaces for M. trifoliata growth and manage about 30cm of water depth control, then habitat creation for M. trifoilata will be successful.

• The Korean Journal of Malacology
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• v.1 no.1
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• pp.24-50
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• 1985

Five different populations of Parafossarulus manchouricus (Chongpyung, Chinju and Kunsan, Korea; and Japan and Taiwan), a population of Bitbynia (Gabbia) misella (Gongju, Korea) and two different populations of Bithynta tentaculata (Michigan, U.S.A. and Bodensee, Germany) were compared in regard to eff-laying characteristics, morphology, chromosome cytology, natural infections of parasites and ecology of habitats. A satisfactory culture method was devised for laboratory rearing of the snails. Tropical fish food (Terra SML) and powdered green leaves (Ceralife) were used as the main food sources for the snails. Benthic diatoms such as Navicula and Gomphonema from the periphyton were also essential for satisfactory growth, especially for the baby snails. The aquaria were stabilized with small stones from a local stream. Young P. manchouricus snails grew to adult size in about 54 days after hatching. They laid eggs 150-156 days after hatching. The whole cycle (birth to egg-laying) took approximately 5 months. The three species of bithyniid snails are iteroparous and lay eggs once a year. There were no major morphological differences in the shells of genera or subgenera studied here. They did exhibit the following rather minor differences. The shell of Parafossarulus has spirally raised ridges, and its apex is usually eroded; the other two genera lack these characteristics. The shell of B. (Gabbia) misella is small, nor exceeding 7.5 mm in length, while the shells of the other two species are larger, being more than 10 mm in length. Scanning electron microscopy (SEM) of the protoconch of P. manchouricus reveals nearly smooth sculpture with small, low, spiral wrinkles. This sculpture is quite different from that of the Hydrobiidae, a family to which the bithyniids are frequently assigned. Scanning electron microscopy of the radulae of the three bithyniid species showed that their radular morphologies are very similar, but there are some small differences, which may be species-specific. There were some statistical differences in shell heights between the Korean and the other populations of P. manchouricus, and between this species and the other two bithyniids as well. The shell differences between the several populations of Korean P. manchouricus may be related to environment. Edtails of the chromosome cycle of these bithyniid snails are similar to those reported for other snails. No specific differences were observed in the chromosome cycle between the various species and populations of snails employed in this study. Reporred for the first time in molluscs are two darkly stained "nucleolar organizers" during pachyterne stages of meiosis. Two different chromosome numbers were observed in the three bithyniid species: n=17 in B. tentaculata and P. manchouricus, and n=18 in B. (G.) misella. no sex chromosomes or supernumerary chromosomes were seen. There were no morphological differences in karyotypes of three Korean strains of P. manchouricus. The infection rates of cercariae of Clonorchis sinensis in Chinju and Kunsan strains of P. manchouricus were 0.14% and 1.25%, respectively. However, Clonorchis cercariae were found in Chongpyung strain of P. manchouriceu and Gongju strain of B. (G.) misella. The habitats of P. manchouricus around Jinyang Lake were relatively clean without any heavy pollution of aquatic microorganisms and organic materials during the period of this study. The levels of dissolved oxygen (D.O.) and biochemical oxygen demand (B.O.D.) of the water specimens sampled from the study areas ranged from 6.0 to 9.6 ppm and from 0.4 to 1.6 ppm, respectively. Eight metalic constituents from the water samples were also assayed, and all metalic ions detercted were remarkably low below the legal criteria. However, calcium ion in the water samples from the habitats of P. manchouricus was considerably higher than others.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.1
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• pp.27-37
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• 2000

This study aims at restoring urban forest destructed and eliminated by industrialization, urbanization, and city development and presenting a direction to manage remaining urban forest ecologically. To this end, an experiment zone where Populus tomentiglandulosa T. Lee were selective cutted and a control zone where Populus tomentiglandulosa T. Lee were kept intact were created in Chongdam Park located in Kangnam-ku, Seoul. Then, the structural changes of herbaceous plant species, the growth of targeted woody plants, and the increase of the number of insect and bird species were examined. The conclusions reached in this study are as follows. First, for the sake of ecological restoration and management of urban forest, it is good to selective cutting. Although timing, frequency, and methods may vary depending on the features and types of urban forest, the study revealed that selective cutting contributes to the restoration speed of origin vegetation and the enhancement of biodiversity including plants and insects. Second, as for the correlations of selective cutting and the appearance of plant species, the growth of origin vegetation, and insect distribution, the study showed that the impact of meteorological environment such as brightness is much greater than that of soil environment. Third, in order to manage urban forest, tramping pressure needs to be controlled efficiently. The efficient control of tramping pressure would contribute in the appearance of herbaceous plants. It would also be beneficial in promoting biodiversity of birds by removing the impact of people using routes. Fourth, in order to enhance the overall biodiversity of urban forest, diverse environment needs to be provided. In particular, it is necessary to supply water that is insufficient in urban forest. Providing habitats such as forest wetland performs an important function to amphibians and birds that require water as well as the appearance of aquatic plants and insects. Therefore, ways to introduce water efficiently should be initiated.

• Parasites, Hosts and Diseases
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• v.21 no.2
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• pp.193-204
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• 1983

The Jinyang Lake is dammed up Nam River, and surrounded by Jinju city and four counties; Jinyang, Sanchong, Hading, and Sachon in kyongsang-Nam-Bo, Korea. The areas around this man-made lake have been known as an endemic focus of clonorchiasis in Korea. The present study was first aimed to know the distribution of freshwater mollusks including medically important snails, and larval trematodes shed from Parafossarulus manchouricus and Semisulcospira libertina. In addition to above studies, water analyses in each snail habitat were carried out in order to figure out a part of their environmental factors. This malaco-ecological survey was done at the sirs areas around upper, middle and lower parts of the lake for 4 months, August-November, 1983. Total nine species of freshwater mollusks were collected throughout the study: 4 species of gastropods: Semisulcospira libertina, Cipangopaludina chinensis, Parafossarulus manchouricus and Radin auricularia, and 5 species of bit.alves; Unio dcuglasiae, Ancdonta woodiana, Lamprotula gottschei, Corbicula yuminea and Limncperma lucustris. Out of nine species of freshwater mollusks, three species of gastropods; S. Zibertina, p. manchouricus and R. auricularia were medically important in terms of the transmission of digenetic trematodes to humans. P. manchouricus and R. auricularia were mainly collected from the shallow ponds and the irrigation channels with the muddy basin, but S. libertine and the bivalves were only collected from the stream of Nam River where the gravels and rocks were dominant. The levels of dissolved oxygen (D.O.) and biochemical oxygen demand \(B.O.D._5)\ of the water specimens sampled from the study areas ranged from 6.0 to 9.6 ppm and from 0.4 to 1.6 ppm respectively. As a result, it is considered that water system around the Jinyang Lake might be relatively clean without any heavy pollution of aquatic microorganisms and organic materials during the period of this study. On the other hand, eight metalic constituents from the water samples were also assayed, and all metalic ions detected were remarkably low below the legal criteria. However, calcium ion in the water samples from the habitats of P. manchouricus was considerably higher than others. Infection rates of digenetic trematodes in the snails were 6.9% in P. manchouricus and 4.8% in S. libertine, respectively. P. manchouricus snails harboring with thc cercariae of Clonorchis sinensis were only 0.14% among the snails examined, and other trematode cercariae except cercaria of C. sinensis were: furcocercus cercariae, cercaria of Loxogenes liberum type I and II. S. libertine snails parasitized with the cercariae of Metagonimus yokogawai were 1, 5% out of the snails examined, and no cercaria of Paragonimus westermani was found in S. libertine snails in the present study. Digenetic trematode cercariae other than M. yokogawai observed in S. libertina snails were: Cercaria yoshidae (B type) , Cercaria cristata, Cercaria innominatum, Cercaria of Centrocestus formosanus and Cercaria nipponensis.

• Journal of Wetlands Research
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• v.18 no.2
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• pp.173-182
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• 2016

Although the ecosystem extends numerous functions for the benefit of humankind, construction of horticultural facilities can potentially lead to the degeneration of some of these functions owing to the expansion of impervious regions and loss of habitats. Thus, this study aimed to examine the effect of construction of horticultural facilities on plant biodiversity. Analysis of the vegetation distribution characteristics showed that horticultural facilities had significantly lower plant diversity than did rice paddies. Hence, the proposed approach involved low-impact development, arrangement of habitat space, plant preservation, restricted use of chemical fertilizer, habitat creation, and reduced preservation cost. Lifestyle analysis suggested the importance of developing favorable environments for the growth of annual plants and aquatic plants. In all, 20 species of naturalized plants belonging to 10 families were identified. Furthermore, the proportion of these naturalized plants was higher in glass greenhouses and multi-span greenhouses, suggesting the advantages of employing natural soil mulching while avoiding concrete mulching. Statistical analysis was performed to validate the results, which suggested that impervious regions be converted to natural soils. Collectively, the findings of this study are expected to be used for establishing policies for the construction of eco-friendly and ecological horticultural facilities; this may aid the maintenance of sustainable agricultural landscapes and large-scale development of the reclaimed lands.

• Journal of Life Science
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• v.26 no.12
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• pp.1446-1457
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• 2016

Wetlands exhibit intermediate characteristics of both terrestrial and aquatic ecosystems, and the biodiversity is rich in these unique biological habitats. The symbiotic relationships between plants and fungi that inhabit these wetlands play an important role in natural resource management, biodiversity, and conservation. Accordingly, the mujechi, having academic value for the study of the natural environment, was investigated in terms of genetic diversity of endophytic fungi, which inhabit the roots of wild plants. The internal transcribed spacer (ITS) region was amplified to identify fungal strains. In total, 226 strains were isolated and categorized into three phyla, seven classes, 10 orders, 22 families, and 31 genera. In plants by endophytic fungi were classified in Isachne globosa (Ig) to 19 genera, Scirpus karuisawensis (Sk) to 11 genera, Utricularia racemosa (Ur) to 19 genera, and one incertae sedis, Eriocaulon decemflorum (Ed) to 11 genera. The fungal taxa was identified the genera Acephala (19.9%), Tolypocladium (16.3%), Neopestalotiopsis (11.5%), and Perenniporia (7.1%). The fungal group isolated from Isachne globosa (Ig) grew the largest number of isolated fungal strains. After comprehensive evaluation, the endophytic fungal group from Utricularia racemosa (Ur) ranked highest in diversity analyses. From the roots of wild plant in mujechi-neup, it confirmed the distribution and diversity of endophytic fungi. This study provides the basic data to understand fungal community structure in peat wetlands.

• Journal of Wetlands Research
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• v.14 no.2
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• pp.255-263
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• 2012

The stream has various environments and they are serving as main habitats of aquatic organisms. The distribution of phytoplankton is affected by water environment, especially pH, nitrogen and phosphorus. To reveal the relationship between phytoplankton distribution and water environment, we measured pH, $NO_3{^-}$ and $PO{_4}^{3-}$ concentration, and abundance of phytoplankton at 10 sites in the upper stream of Dorim-cheon. pH value ranged 5.05 to 7.56. $NO_3{^-}$ and $PO{_4}^{3-}$ concentrations ranged 0.4 ~ 4.9ppm and 0.02 ~ 0.99ppm, respectively. A point source of $NO_3{^-}$ was Seoul National University but concentration was not high and dropped to normal range at 400m downstream. $NO_3{^-}/PO{_4}^{3-}$ ratio ranged 28 to 152 except site 4 (0.4) where was affected by $PO{_4}^{3-}$ point source. Water pH, $NO_3{^-}$ and $PO{_4}^{3-}$ concentrations increased with downstream and were related to the input of irrigation water from Han-river between site 5 and 6. Bacillariophyceae alge dominated this stream. Phytoplankton density increased abruptly at downstream of site 5. In general, phytoplankton density did not increase until the $NO_3{^-}$ concentration of 3.5ppm and $PO{_4}^{3-}$ concentration of 0.07ppm. Phytoplankton density was low at sites where $NO_3{^-}/PO{_4}^{3-}$ ratio was larger than 50.