• The Plant Pathology Journal
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• v.34 no.3
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• pp.191-198
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• 2018

Fast and accurate diagnosis is needed to eradicate and manage economically important and invasive diseases like fire blight. Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. Primers for new LAMP assay (named as DS-LAMP) were designed from a histidine-tRNA ligase gene (EAMY_RS32025) of E. amylovora CFBP1430 genome. The DS-LAMP amplified DNA (positive detection) only from genomic DNA of E. amylovora strains, not from either E. pyrifoliae (causing black shoot blight) or from Pseudomonas syringae pv. syringae (causing shoot blight on apple trees). The detection limit of DS-LAMP was 10 cells per LAMP reaction, equivalent to $10^4$ cells per ml of the sample extract. DS-LAMP successfully diagnosed the pathogens on four fire-blight infected apple and pear orchards. In addition, it could distinguish black shoot blight from fire blight. The $B{\ddot{u}}hlmann$-LAMP, developed previously for on-site diagnosis of fire blight, did not give consistent results for specificity to E. amylovora and on-site diagnosis; it gave positive reactions to three strains of E. pyrifoliae and two strains of P. syringae pv. syringae. It also, gave positive reactions to some healthy sample extracts. DS-LAMP, developed in this study, would give more accurate on-site diagnosis of fire blight, especially in the Republic of Korea, where fire blight and black shoot blight coexist.

• Korean Journal of Environmental Agriculture
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• v.41 no.3
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• pp.199-205
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• 2022

BACKGROUND: The freezing injury by pruning can be reduced by suspending pruning work when severe cold weather (-23 to -49℃) is forecast. Minimum air temperature of the study area, Gunwi region at February 3, 2012 was -21.9℃, and the subzero temperature continued until April 8, 2012. This study was conducted in two years to investigate the effect of delaying winter pruning until full bloom on shoot growth and fruit quality of 'Fuji'/M.9 apple trees. METHODS AND RESULTS: The time of pruning were March 26 for dormant, April 3 for bud break, and May 2 for full bloom. The winter pruning at full bloom significantly reduced fruit weight for two years compared with the control (winter pruning at dormant), and shoot growth was reduced only in the following year. There was no significant effect of delaying winter pruning at bud break on soluble solid content, fruit red color, return bloom, and pruning weight for two years compared with the control. CONCLUSION(S): These results indicated the delaying winter pruning at bud break of 'Fuji'/M.9 apple tree did not offer any disadvantage over comparable dormant pruning, since the fruit quality was not affected. The delayed pruning at full bloom resulted in decreased fruit weight, though shoot growth, fruit quality, and return bloom were not affected by the delayed pruning. So, the delayed pruning should be considered carefully only for the fruit tree orchards in diseases.

• Korean Journal Plant Pathology
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• v.13 no.3
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• pp.145-151
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• 1997

An unusual young fruit rot of apple caused by two species of Phytophthora was epidemic from late May to early July of 1996 in Andong, Uisung and Chungwon areas of Korea. The disease spread to over 30 apple orchards in the areas and percent of the infected tree and fruit was ca. 10~90% and 1`15%, respectively. Water soaking lesions or spots on leaves and shoot blight were also developed by the pathogen. Among 39 isolates collected, 25 were identified as P. cactorum and the others were as zp. cambivora on the basis of their distinctive morphological characters. While the former fungus was homothallic, all isolates of the latter were A1 mating types. Koch's postulate was fulfilled. Both fungi showed strong pathogenicity not only to young fruits, leaves and shoots of apple but also to those of pear and peach. Several vegetables tested did not show symptoms even by wound inoculation. An Occurrence of young fruit rot of apple caused by Phytophthora has not been reported in Korea, especially, P. cambivora has not been recorded previously as the causal agent of the disease in the world.

• Korean Journal of Plant Resources
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• v.37 no.4
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• pp.401-410
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• 2024

This study was undertaken to elucidate the effects of virus and viroid infections on the growth of trees and the attributes of fruit quality in 'Hongro' apples. Trials were initiated using virus-infected, viroid-infected, combined virus/viroid-infected, and uninfected apple trees in an experimental apple orchard at the National Institute of Horticultural and Herbal Science in 2019. The growth of each tree was measured annually and compared between virus-free and virus/viroid-infected trees. Fruits were harvested from all apple trees, and selected attributes of fruit quality, including yield, weight, firmness, titratable acidity, and anthocyanin content, were determined in September 2021-2022. The results revealed significant differences among virus-free trees and those infected with either virus, viroid, or a combination of virus and viroid. Infection with viral and viroid diseases led to reductions in tree height (14.0%), trunk area (23.1%), fruit yield (65.0%), fruit weight (34.4%), and anthocyanin content (39.8%), while increasing fruit firmness (33.2%) and titratable acidity (39.8%), respectively. We anticipate that our research findings will also be beneficial for apple virus and viroid disease control, as well as apple cultivation management.

• Journal of Broadcast Engineering
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• v.25 no.2
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• pp.208-217
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• 2020

The classification of plant diseases by images captured by a camera sensor has been studied over past decades. A method that has gained much interest is to use image segmentation, from which statistical features are derived and analyzed by machine learning. Recently, deep learning has been adopted in this area. However, image segmentation is still a difficult task to achieve stable performance due to a variety of environmental variations. The end-to-end learning in neural network has a demerit that train images may be different from real images acquired in outdoor fields. To solve these problems, we propose superpixel-based disease classification method using end-to-end CNN (convolutional neural network) learning. Based on experiments performed on PlantVillage apple images, the classification accuracy is 98.29% and 92.43% for full-image and superpixel. As well, the multivariate F1-score is (0.98, 0.93). Therefore we validate that the method of using superpixel is comparable to that of full-image.

• The Korean Journal of Community Living Science
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• v.20 no.1
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• pp.5-17
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• 2009

The objective of this study was to analyze the factors regarding work-related musculoskeletal disorders (MSDs) in fruit-growers and offer the fundamental data for work improvement. The 587 fruit-growers (409 males and 178 females) working with 5 kinds of fruit: apple, pear, peach, grape, and mandarin participated in a questionnaire survey regarding MSDs and health condition in 2004 and 2005. The results are as follows: 1. Smoking rate was highest in apple growers. 2. Drinking rates were highest in male peach growers and in female grape growers. 3. Regular exercise rates were higher in pear and mandarin growers. 4. Physical and mental fatigue was higher in females than in males in most cases. 5. The prevalence rate of medically diagnosed diseases was highest for osteoarthritis (16.4%), herniated nucleus pulposus (HNP, 13.5%), and chronic gastritis/gastric ulcer (10.5%). As well as, the prevalence of dermatosis was higher in pear and mandarin growers. 6. The prevalence rate of musculoskeletal symptoms among the various pain areas was highest for lower back, shoulders, and knees. The prevalence rate was also high for lower back, shoulder, and knee pain in apple and grape growers. 7. The significant indexes used determining the musculoskeletal symptoms were BMI, working period, and regular exercise. These results can be practically used for work improvement for the fruit-growers to prevent MSDs.

• Korean Journal of Organic Agriculture
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• v.7 no.2
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• pp.99-106
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• 1999

In order to acquire microbial agents that can be utilized for biological control of bitter rot(Glomerella cingulata), the major airborne disease to apple, the effective microorganisms were isolated, tested for antagonistic activity to the pathogen. Through the screening of more than 1,000 species of microorganisms collected in nature, 11 species of antagonists were selected. On of the 11 species, one species designated as CH141 demonstrated outstanding activity. The bacterial strain, CH1141 exerted antagonistic efficiency of 65% on Glomerella cingulata. The CH1141 was identified as a bacterial strain to Bacillus subtilis based on morphology, culture conditions, and physiobiochemical characteristics.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.133.2-134
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• 2003

Cedar-apple rust fungi had been collected at 36 sites throughout the country from 1984 to 2001 and deposited at the Herbarium of Korea Forest Research Institute (HKFRI). We conducted the morphological examination on the dried specimens by light and scanning electron microscopy and as results six Gymnosporangium species were identified. Three species, G. asiaticum, G. clavaritforme and G. yamadae, were previously described in Korea, while the other three species, G. cornutum, G. globosum, and G. japonicum were new to Korea. Here we present the detailed morphological descriptions, distribution, host ranges and keys to species in both aecial and telial stages of each species. Some morphological characteristics related with telial formation on trees were newly identified; witches brooms for G. asiaticum, small galls for G. yamadae and telial formations on trunk for G. japonicun Geographically G. asiaticum and G. yamadae distributed widely throughout Korea, while the others were collected only at the limited locations. Eight Juniperus species as telial hosts and fifteen Rosaceous plants as aecial hosts were confirmed to be new in Korea.

• Korean Journal Plant Pathology
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• v.11 no.2
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• pp.132-138
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• 1995

우리나라 사과원에서의 토양병 발생현황과 발생실태를 1991년부터 1993년까지 3년간 조사한 결과, 전체 조사주수의 약 8.1%가 각종의 토양 병원균에 감염되어 있는 것으로 나타났다. 토양병 중 자주날개 무늬병의 발생빈도가 5.1%로 가장 높게 조사되었으며, 흰날개무늬병과 역병의 이병주율은 각각 2.5%와 0.2%로 조사되었다. 자주날개무늬병에 감염된 나무에서는 잎의 황화 및 조기낙엽, 신초생장 억제, 과실 생육 불량 등의 지상부 병징이 관찰되었고, 지하부 뿌리 표면에는 적자색 실모양의 균사나 균사속이 관찰되었다. 감염된 뿌리의 표피는 쉽게 벗겨지고 목질부로부터 잘 이탈되는 특징이 있었다. 흰날개무늬병균에 감염된 나무의 뿌리는 흰색의 균사 혹은 균사막으로 싸여 있으며 이 균사막은 시간이 경과하면 회색 내지 흑색으로 착색되었다. 굵은 뿌리의 표피를 제거하면 목질부에 백색 부채모양의 균사막과 실모양의 균사속을 확인할 수 있었다. 이 병원균은 목질부까지 부패시키므로 병의 증세가 심하게 나타났다. 자주날개무늬병은 새로 개원한 10년 이하의 과원에서 발생이 많았다. 자주날개무늬병은 왜화도가 높은 대목에서 발병율이 높았으나 흰날개무늬병은 대목의 종류에 관계없이 발명되었다. 자주날개무늬병은 식양토 과원에서 발생이 많았으며 흰날개무늬병은 사양토 과원에서 발생이 많았다.

• The Plant Pathology Journal
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• v.36 no.6
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• pp.570-578
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• 2020

Rhizopus rot is a serious postharvest disease of various crops caused by Rhizopus spp. and controlled mainly by synthetic fungicides. We detected the antifungal activity of a culture extract of Setosphaeria rostrata F3736 against Rhizopus oryzae. The active ingredient was identified as moriniafungin, a known sordarin derivative, which showed minimum inhibitory concentrations of 1-8 ㎍/ml against Colletotrichum spp. and 0.03-0.13 ㎍/ml against Rhizopus spp. in vitro. Moriniafungin showed protective control efficacies against Rhizopus rot on apple and peach fruits. Treatment with 25 ㎍/ml moriniafungin delimited the lesion diameter significantly by 100% on R. oryzae-inoculated apple fruits compared with the non-treated control. Treatment with 0.04 ㎍/ml of moriniafungin reduced the lesion diameter significantly by 56.45%, and treatment with higher concentrations of 0.2-25 ㎍/ml reduced the lesion diameter by 70-90% on Rhizopus stolonifer var. stolonifer-inoculated peach fruit. These results suggest moriniafungin has potential as a control agent of postharvest diseases caused by Rhizopus spp.