• The Plant Pathology Journal
• /
• v.39 no.1
• /
• pp.88-107
• /
• 2023

In the present investigation, bacterial isolates from infected apple trees causing apple canker during winter were studied in the northern Gyeongbuk Province, Korea. The pathogen was identified as Pseudomonas syringae pv. syringae (Pss) through various physiological and biochemical characterization assays such as BIOLOG, gas chromatography of fatty acid methyl esters, and 16S rRNA. Bioassays for the production of phytotoxins were positive for syringopeptin and syringomycin against Bacillus megaterium and Geotrichum candidum, respectively. The polymerase chain reaction (PCR) method enabled the detection of toxin-producing genes, syrB1, and sypB in Pss. The differentiation of strains was performed using LOPAT and GATTa tests. Pss further exhibited ice nucleation activity (INA) at a temperature of -0.7℃, indicating an INA⁺ bacterium. The ice-nucleating temperature was -4.7℃ for a non-treated control (sterilized distilled water), whereas it was -9.6℃ for an INA^- bacterium Escherichia coli TOP10. These methods detected pathogenic strains from apple orchards. Pss might exist in an apple tree during ice injury, and it secretes a toxin that makes leaves yellow and cause canker symptoms. Until now, Korea has not developed antibiotics targeting Pss. Therefore, it is necessary to develop effective disease control to combat Pss in apple orchards. Pathogenicity test on apple leaves and stems showed canker symptoms. The pathogenic bacterium was re-isolated from symptomatic plant tissue and confirmed as original isolates by 16S rRNA. Repetitive element sequence-based PCR and enterobacterial repetitive intergenic consensus PCR primers revealed different genetic profiles within P. syringae pathovars. High antibiotic susceptibility results showed the misreading of mRNA caused by streptomycin and oxytetracycline.

• Korean Journal Plant Pathology
• /
• v.11 no.1
• /
• pp.9-16
• /
• 1995

Undiluted neoasozin solution (6.5% a.i.), an arsenic fungicide, was sprayed on 169 cankers of apple trees from early March to September in 1987 twice at intervals of one week without scraping off the affected barks. Among the treated cankers, 79.9% ceased to grow within 1∼7 weeks, 13.0% showed partial development, and 7.1% grew continuously to girdle the branches. The partially developed cankers, however, could also be cured by an additional spray after slightly piercing at the edge of cankers to facilitate the penetration of the chemical. When the canker growth was blocked, cankers were encircled by cracks developed at the marginal area of the cankers. If the cracks developed once, very few cankers grew beyond them. The above results suggest that the crack development may be the consequence of the host defense activity to wall off the pathogen. In addition to the curative efficacy, the neoasozin solution inhibited sporulation of the pathogenic fungus almost completely. However, the pathogen survived for more than three months in some cankers that externally appeared to be cured, suggesting that an indirect mode of action of the chemical against apple Valsa canker seems to be still more persuasive than the direct fungicidal effect. In the final examination conducted in the mid April of the next year, 72.7% of the cankers were completely cured by the two successive neoasozin treatments. Moreover the cure rate became 83.1% if that of partially developed cankers which were also completely cured by an additional treatment was also taken into account. Since 1989 when this method was widely applied in apple orchards in Korea, apple Valsa canker has been effectively controlled to reach a tolerable level.

• Mycobiology
• /
• v.28 no.3
• /
• pp.153-157
• /
• 2000

In this paper, the distribution, host restriction, and taxonomic position of Valsa mali are discussed.

• Korean Journal of Organic Agriculture
• /
• v.6 no.1
• /
• pp.35-43
• /
• 1997

For the purpose of acquiring microbial agents that can be utilized to billogically control the major airborne disease to apple trees, such as canker(Valsa ceratosperma), the effective microorgaisms were isolated, tested for antagonistic activity to the pathogen causing major disease to apple trees and identifed. Screening of more than 3, 000 species of microorganisms collected in nature for them antagonistic action to the pathogen, Valsa ceratosperma causing disease to apple tree resulted in selection of effective species. Out of the 11 species, one species designated as CAP141 demonstrated outstanding activity. The bacterial strain, CAP141 exerted antagonistic efficiency of 65% on Valsa ceratosperma. The CAP141 was identified as a bacterial strain to Bacillus subtilis based on morphology, culture conditions, and physio-biochemical characteristics.

• Korean Journal of Organic Agriculture
• /
• v.25 no.1
• /
• pp.125-134
• /
• 2017

This study was carried out to examine the effects of soil sulfur mixture on the control apple valsa canker in apple orchard for two years. In organic apple orchard, The recovery percentages of apple valsa canker were compared among control (no treatment), lime sulfur, and soil sulfur in organic orchards. In conventional apple orchards, those of recovery percentages were compared among control, neoasozin, lime sulfur, and soil sulfur. Compared with control, soil sulfur treatments significantly improved the recovery percentages of apple valsa canker infected trees in organic orchard. However, there were no differences between lime sulfur and soil sulfur treatment in organic apple orchard, except BongHyun orchard experiment in 2013. Compared with control fruit qualities, fruit skin red color and fruit firmness were improved in lime sulfur and soil sulfur treatment, respectively. In conventional orchard, apple trees treated with lime sulfur, soil sulfur or neoasozin improved recovery percentages, compared with those of control trees. Soil sulfur treatments recovered 87.5~97.5% of infected 'Fuji'/MM106 apple trees in organic and conventional apple orchards. The trees applied with neoasozin showed significant lower shoot growth than those of soil sulfur treatment in conventional orchard. Soli sulfur treatment improved fruit red color, but did not affected fruit weight, fruit firmness, soluble solids concentrations, and titratable acidity.

• Korean journal of applied entomology
• /
• v.9 no.2
• /
• pp.81-84
• /
• 1970

The occurence of apple canker in the three main apple growing areas including Yesan, Chungju and Taegu was investigated during the four years from 1967 to 1970. According to the survey, about 30 percent of apple plants was infected with canker organisms and more the disease occurred in older plants in comparison with younger ones. Jonathan was said to be highly susceptible among five varieties observed. Main trunks or main branches had more canker lesions than those of twigs or of side branches. The survey showed that application of higher nitrogenous fertilizer without boric acid predisposed to the disease together with sandy soil.

• Korean Journal of Organic Agriculture
• /
• v.5 no.1
• /
• pp.137-147
• /
• 1996

For the purpose of acquiring microbial agents that can be utilized to biologically control the major airborne diseases to apple trees, such as canker(Botryosphaeria dothidea), bitter rot(Glomerella cingulata), alternaria leaf spot(Alternaria mali), root rot(rosellinia necatrix), canker(Valsa ceratosperma) and gray mold rot(Botrytis cinerea), the effective microorgaisms were isolated, tested for antagonistic activity to the pathogens causing major diseases to apple trees and identifed. Screening of more than 5,000 species of microorganisms collected in nature for them antagonistic action to the pathogens causing 5 major diseases to apple trees resulted in selection of effective species. Out of the 11 species, one species designated as CAP134 demonstrated outstanding activity. The bacterial strain, CAP134 exerted antagonistic efficiency of 57% on an isolated strain and 40% on a donated strain of Botryosphaeria dothidea., 52% on an isolated strain and 46% on a purchased strain of Alternaria mali, 60% on Valsa ceratosperma 25% on Glomerella cingulata, and 64% Rosellinia necatrix. The CAP134 was identified as a bacterial strain to Bacillus subtilis ATCC 6633 based on morephology, culture conditions, and physio-biochemical characteristics.

• Korean journal of applied entomology
• /
• v.11 no.1
• /
• pp.19-23
• /
• 1972

Apple cankers caused by Valsa mali Miyabe, Botryosphaeria ribis Cross, Phomopsis truncicola Miura, are distributed in the main apple growing areas, Choong Chung Puk Do, Choong Chung Nam Do, and Kyung Sang Puk Do. According to the survey in 1970 that Valid mali is the dominant causal organism among the three in the most severely damaged area. Valsa mali and Botryosphaeria ribis were found in ail 3 provinces, however, Phomopsis truncicola was not found in Choong Chung Nam Do. Especially, Botryosphaeria ribis was most abundant in Chunwon of Choong Chung Nam Do. Effects of chemical control of apple canker caused by Valsa mali were tested both on potato dextrose agar with inhibition zones produced by the fungicides and relative growth of assay fungus of peridermis was treated with tile fungicides. Ifost effective was mercuric and followed by Lime sulfur, Hydroquinone sulfate, in general.

• Korean journal of applied entomology
• /
• v.23 no.2 s.59
• /
• pp.82-88
• /
• 1984

Fruit rot and blister canker, a disease of apple occurring severely in Korea has been studied for correct identification of the syndrome In fruit and apple trees. Among the fungi isolated from blister cankers, rough barks or fruits showing rotting of 7 different host species were Botryosphaeria berengeriana (pycnidial stage. Dethiorella mali), Penicillium expansum and Alternaria sp. from apple rots and Phomopsis sp. from pear fruit rots. The most dominant isolates were B. berengeriana. Ten isolates of D. mali were grouped in to two conidial types based up mycelial growth rate, growth habits and mycelial coloration on PDA. None of 10 isolates was chromogenic. Pycnidia in apple stems, stromatic, dark brown, globose or subglobose and the measuring were $103.5-287.5{\mu}\times92.0-287.5\mu$. The pycnidia contained hyaline, nonseptate, fusiform conidia. The sizes of pycnidiospore of isolates obtained from apple twig were $4.3-7.2{\mu}\times20.0-31.5{\mu}(average\;5.9\times25.4\mu)$. Some conidia of this fungus from apple, pear, peach and ornamental cherry showed 1-,2-,3-septate before or during germination. Microconidia were observed in pycnidia on PDA and fruit lesion of inoculated host. Symptoms on leaves and fruits were contoured brown spots when inoculated. Wart-like protuberance were formed on the surface of apple and pear. Canker appeared on branches of peach and ornamental cherry inoculated.

• Korean Journal of Environmental Agriculture
• /
• v.41 no.4
• /
• pp.328-334
• /
• 2022

BACKGROUND: The low temperature at flowering period break the balance between vegetative and reproductive growth of apple tree. Summer pruning has been used to control vegetative growth. So, this study was conducted to investigate the effect of summer pruning time on shoot growth and fruit quality of 'Fuji'/M.9 apple trees damaged by the low temperature at flowering period. METHODS AND RESULTS: The following treatments were applied to tree : a) control (no summer pruning), b) pruned 26 June, c) pruned 30 July, d) pruned 28 August, and e) pruned 26 September. The summer pruning significantly increased light penetration and fruit red color by reducing the total shoot growth compared with control. And the summer pruning control the outbreak of apple valsa canker. But the summer pruning at the end of June increased regrowth of shoot and pruning weight compared with the summer pruning at the end of August. The summer pruning at 30 July had the highest fruit weight, but return bloom was the highest in the summer pruning at 28 August. CONCLUSION(S): These results indicated the optimum summer pruning time of 'Fuji'/M.9 apple trees damaged by the low temperature at flowering period were the end of August.