• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2001년도 합동 춘계 학술발표회
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• pp.59-59
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• 2001

• The Korean Journal of Ecology
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• 제20권5호
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• pp.375-384
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• 1997

The effects of three different irrigating water qualities(clean water, life sewage and animal sewage) on the pattern of arthropod communities in the rice fields were investigated at Banwol, Kyonggi-do, mid-western part of Korea from 1994 to 1996. The total density of arthropod was highest in the rice field which was irrigated with clean water(clean water field), and the arthropod community was mainly composed of aphids and collembolans. Each functional group was found in the order of 'pests>non-pests>natural enemies' in its density. The dominant taxa in the pest group were aphids, planthoppers(Delphacidae), leafhoppers(Cicadelidae) and rice water weevil(L. oryzophilus). In the non-pest group collembolans, non-biting midges and dipterans were main arthropods, and in the natural enemy group Araneae was the dominant taxon. In the early growth stage of rice plant the dominant functional group was pests, and in the mid growth stage both the pest and the natural enemy group became dominant. But in the late growth stage none of the functional group was dominant over the other functional groups. The densities of spiders were much more higher in the clean water field than in the other fields. And through all the growth stages of rice plants the density of spiders in clean water field kept increase in contrast to the others. The species composition of spiders in the life sewage field was similar to that in the animal sewage field, but in the clean water field it was different from the other two fields.

• 농업과학연구
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• 제37권3호
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• pp.361-365
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• 2010

For the development of more effective insecticides for aphids, including the cotton aphid, Aphis gossypii Glover, control released insecticides were developed and tested as new type of formulation for the cotton aphid. Etofenprox is a target-of-control-released formulation that is toxic to insects with piercing-sucking type mouths including aphids and hoppers. The insecticidal effect of etofenprox is distinguished among others; however, continuance period of the effect of insecticide is shorter than other insecticides. In addition, the control effect of control released etofenprox on the cotton aphid was examined. The results show that control released etofenprox induced no control effects to the cotton aphid within 5 days of treatment. The cotton aphid's population was increased, and their reproduction rate was also high. However, control effect was shown to have very low levels 7 days after treatment, and it increased to higher effect. It is supposed that it is possible to control the cotton aphid using the type of control released etofenprox with a kitosan carrier. It is necessary to develop a more effective carrier type to release more of the insecticidal ingredient on the leaf after spraying.

• 한국응용곤충학회지
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• 제34권2호
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• pp.100-105
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• 1995

정확한 in vitro 전사가 일어날 수 있는 진딧물의 세포추출액을 제조하였다. 전사를 직접 조절할 수 있는 단백질 인자를 규명하기 위하여 전사개시점과 그의 상류에 결합하는 DNA 결합단백질을 탐색했다. 전사개시점을 포함하는 단편 A(-194/23)에는 52kDa, 50kDa, 40kDa의 단백질들이 결합했으며 전사개시점 상류의 DNA 단편 B(-393/-263)에는 52kDa, 50kDa, 40kDa의 단백질들이 결합한 반면 DNA 단편 C(-263/-195)는 53kDa단백질만이 결합했다. 그리고 이들 DNA 결합단백질들의 DNA 결합 활성에는 양이온이 요구되었다.

• 농업과학연구
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• 제44권3호
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• pp.348-358
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• 2017

The green peach aphid, Myzus persicae, is one of the most serious insect pest and a vector for a multitude of viral diseases to many crops, vegetables, ornamentals, and fruit trees in the world. A large number of aphids can reduce plant vigor and cause defoliation. Many insecticides have been developed and applied to control the green peach aphid. However, this aphid has displayed a remarkable ability to establish resistance to almost every insecticide. We treated 5 different insecticides registered for M. persicae on pepper leaves and investigated the effects of the insecticides by measuring the time it took to achieve 90% control of the aphids. Acetamiprid worked faster than any other insecticides while cyantraniliprole showed the slowest insecticidal effect. Pymetrozine, pyrifluquinazon, and spirotetramet provided 90% control within similar time. Iwol population's control value was higher than any other populations 24 hours after treatment. When five different unregistered insecticides for M. persicae were treated on pepper leaves, no insecticidal effect was found for gamma-cyhalothrin and novaluron and spinosad showed an insecticidal effect of up to 70% in Iwol population only. Although chlorfenapyr and dinotefuran were not registered for M. persicae, their insecticidal effects were found to be 90% or higher.

• 한국식물병리학회지
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• 제14권2호
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• pp.150-156
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• 1998

To develop a model for prediction of turnip mosaic virus(TuMV) disease progress of Chinese cabbage based on weather information and number of TuMV vector aphids trapped in Taegwallyeong alpine area, data were statistically processed together. As the variables influenced on TuMV disease progress, cumulative portion(CPT) above 13$^{\circ}C$ in daily average temperature was the most significant, and solar radiation, duration of sunshine, vector aphids and cumulative temperature above $0^{\circ}C$ were significant. When logistic model and Gompertz model were compared by detemining goodness of fit for TuMV disease progress using CPT as independent variable, regression coefficient was higher in the logistic model than in the Gompertz model. Epidemic parameters, apparent infection rate and initial value of logistic model, were estimated by examining the relationship between disease proportion linearized by logit transformation equation, In(Y/Yf-Y) and CPT. Models able to describe the progression of TuMV disease were formulated in Y=100/(1+128.4 exp(-0.013.CPT.(-1(1/(1+66.7.exp(-0.11.day). Calculated disease progress from the model was in good agreement with investigated actual disease progress showing high significance of the coefficient of determination with 0.710.

• 생물환경조절학회지
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• 제13권1호
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• pp.56-60
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• 2004

진딧물 포식성 무당벌레의 성충을 대상으로 저온처리에 의한 생식적 휴면을 유도하였다. 성충의 인위적인 생식적 휴면의 유지는 $4^{\circ}C$에서 5개월 정도까지 가능하며 먹이의 공급이 필요하지 않아 경제적인 무당벌레의 저장법이라 할 수 있다. 하절기를 포함한 계절에 구분 없이 무당벌레의 성충의 저온저장이 가능하여 필요시 어느 때라도 진딧물 발생지에 무당벌레를 공급할 수 있게 되었다. 휴면 후 $25^{\circ}C$에서 수명과 산란력이 우수하며 생식호르몬인 juvenile hormone(JH)의 합성 유사체인 methoprene은 암컷의 초반기 생식력을 증진시키는 효과를 나타낸다.

• Mycobiology
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• 제46권4호
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• pp.440-447
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• 2018

Dual biocontrol of both insects and plant pathogens has been reported for certain fungal entomopathogens, including Beauveria bassiana and Lecanicillum spp. In this study, we demonstrate, for the first time, the dual biocontrol potential of two fungal isolates identified by morphological and phylogenetic analyses as Isaria javanica. Both these isolates caused mortality in the greater wax moth, and hence can be considered entomopathogens. Spores of the isolates were also pathogenic to nymphs of the green peach aphid (Myzus persicae), with an $LC_{50}$ value of $10^7spores/mL$ 4 days after inoculation and an $LT_{50}$ of 4.2 days with a dose of $10^8spores/mL$. In vitro antifungal assays also demonstrated a strong inhibitory effect on the growth of two fungi that are pathogenic to peppers, Colletotrichum gloeosporioides and Phytophthora capsici. These results indicate that I. javanica isolates could be used as novel biocontrol agents for the simultaneous control of aphids and fungal diseases, such as anthracnose and Phytophthora blight, in an integrated pest management framework for red pepper.

• The Plant Pathology Journal
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• 제38권2호
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• pp.159-166
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• 2022

Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat samples based on the conserved sequences of the BYDV coat protein gene. Specific primers and a probe for RT-RPA reacted and optimally incubated at 42℃ for 10 min, and the end-labeled amplification products were visualized on LFS within 10 min. The RT-RPA-LFS assay showed no cross-reactivity with other major cereal viruses, including barley mild mosaic virus, barley yellow mosaic virus, and rice black streaked dwarf virus, indicating high specificity of the assay. The sensitivity of the RT-RPA-LFS assay was similar to that of reverse transcription polymerase chain reaction, and it was successfully validated to detect BYDV in oat samples from six different regions and in individual aphids. These results confirm the outstanding potential of the RT-RPA-LFS assay for rapid detection of BYDV.

• 한국응용곤충학회지
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• 제62권4호
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• pp.345-346
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• 2023

스마트팜형 시설 딸기에 예찰 없이 작물 정식 초기에 천적을 먼저 적용하는 생태공학적 Natural Enemy in First (NEF) 기법이 총채벌레류와 진딧물류의 밀도에 미치는 영향을 확인하였다. 대조구는 약제를 처리하여 비교하였다. NEF 처리구에서 총채벌레류와 진딧물류의 천적과 서식처로 참멋애꽃노린재와 Portulaca sp.를 적용하여 작기 종료시점까지 해충의 밀도를 대조구와 유사하게 효과적으로 관리할 수 있었다.