• 한국약용작물학회지
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• 제7권1호
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• pp.37-44
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• 1999

60종(種)의 약용식물과 식용작물을 대상으로 항종양활성등의 생리활성을 조사함으로서 약용식물과 식용작물의 유용적인 측면의 확인뿐만 아니라 나아가서 새로운 생리활성물질 탐색의 가능성을 검토하기 위해서 실험하였으며 결과는 다음과 같다. 약용식물 및 식용작물에 대한 80% EtOH 추출물을 이용하여 항종양효과를 보면 PKC법에서는 명아주(73.4%) 및 antibleb형성억제력검정에서는 검정콩이, PLC법에서는 검정콩(91.9%), MTT법에서 50%의 억제력을 나타내는 농도$(IC_{50})$가 검정콩과 쑥의 경우 각각 $4.7{\mu}g/ml$을 보였다.

• Archives of Pharmacal Research
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• 제15권4호
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• pp.356-359
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• 1992

The activity-oriented fractionation of Psoralea corylifolia led to an isolation of a (+) bakuchio 1 as an activ eprinciple of its antitumoral property in vitro 1 was observed to exhibit a mild cytotoxicity against five kinds of cultured human cancer cell lines, i. e. the A549, SK-OV-3, SK-MEL-2, XF-498 and HCT15. The synthesized 2, 3-epoxide of (+)-bakuchiol 3 showed the similar activity as the (+) bakuchiol 1, whereas the other oxidation derivatives 4 and 5 including the acetyl (+) bakuchiol 2 showed a decreased activity.

• 대한한방내과학회지
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• 제19권2호
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• pp.219-232
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• 1998

Paljintanggagmbang has been used for cure of tumor as a traditional medicine without any experimental evidence to support the rational basis for its clinical use. This study was carried out to evaluate. the possible therapeutic or antitumoral effects of Paljintanggagmbang extract against tumor, and to carry out some mechanisms responsible for its effect. Experimental studis were performed for measurement of Humoral and Cellular Immune Response and Phagocytosis in Mice treated with mitomytion C(MMC) and Paljintanggagmbang alone and combination. The results obtained in this study were as follows 1. The adminstration of Paljintanggagmbang extract decresed size of tumors cell which MCA induced. 2. The adminstration of Paljintanggagmbang extract decresed growth of the tumors which S 180 transplant. 3. The adminstration of Paljintanggagmbang extract decresed reproduction of A549, Hep3b, 3LL cell and S 180 in vivo. 4. The adminstration of Paljintanggagmbang extract incresed activity of the NK cell. These results also suggested that effect of Paljintanggagmbang might be chiefly due to nonspecific enhancement of Humoral and Cellular Immune Response and Phagocytosis in Mice treated with MMC and Paljintanggagmbang alone and combination.

• Journal of Photoscience
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• 제9권2호
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• pp.524-526
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• 2002

Hypericin was found to exhibit the highest antitumoral activity in treating EAT by photodynamic therapy (PDT): Hypericin>HPde>PII>TPPS$_4$>ALA. Moreover, 25% of mice after Hyp-based PDT survived 4 months, if compare with control group. Antitumor activity of these photosensitizers was in rather clear correlation with accumulation potential.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2765-2770
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• 2012

Background: There is a long standing interest in the identification of medicinal plants and derived natural products for developing cancer therapeutics. Here we investigated the antiproliferative properties of Melissa officinalis (MO) from Turkey on breast cancer. Methods: MO extracts were studied for cytotoxicity against breast cancer cell lines (MCF-7, MDA-MB-468 and MDA-MB-231). In vitro apoptosis studies were performed by annexin V staining and flow cytometry analyses. Immunohistochemistry for Ki-67 and caspase 7 in the tumoral tissue sections of DMBA-induced mammary tumors in rats was also performed, along with TUNEL assays to detect apoptotic cells. In vivo anticancer activity testing was carried out with reference to inhibition of growth of DMBA induced mammary tumors in rats. Results: MO showed cytotoxicity against three cancer cell lines, inducing increase in Annexin-positive cells. Expression of caspase-7 protein and TUNEL positive cells were much higher in rats treated by MO, compared with the untreated control group, while expression of Ki-67 was decreased. Furthermore, in vivo studies showed that mean tumor volume inhibition ratio in MO treated group was 40% compared with the untreated rats. Conclusion: These results indicated that MO extrcts have antitumoral potential against breast cancer.

• 한국축산식품학회지
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• 제23권1호
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• pp.80-85
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• 2003

티벳산 발효유로부터 분리한 Candida kefyr TFP7의 항균활성 및 항암활성을 검토하기 위하여 PDA배지를 이용한 십진 희석법에 의해 10균주(TFP1∼10)를 분리하여 몇 종의 병원균과 식중독균, 식품과 관련이 있는 세균, 효모, 곰팡이, 녹조류 및 토양으로부터 분리한 방선균 등 18균주에 대하여 항균활성을 조사하였으며, 또한, 9종류의 인체 암세포주에 대하여 항암활성을 비교하였다. 항균활성 검사에서는 Gram 양성균 중 iwicrococcus iuteus ATCC l1880에 대하여 분리균주 (TFP2∼10)와 방선균인 Styeptomyces murinus JCM 4333에 패하여 분리균주(TFP6∼10)가 뚜렷한 생육억제효과를 나타내었다. 한편, 항암활성 검사에서는 SNU-5(human stomach carcinoma), SW-534(human larynx carcinoma)에 대하여 분리 균주(TFP1∼10) 모두 각각 60%, 70%이상의 생육억제 효과를 나타내었다. 특히, 균주 TFP7은 SNU-5, SW-534에 대하여 각각 77.5%, 76.5%의 가장 우수한 생육억제 효과를 나타내었다.

• Bulletin of the Korean Chemical Society
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• 제25권2호
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• pp.195-197
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• 2004

Methyl-4-[[(2E)-3,7-dimethyl-2,6-octadienyl]oxy]-3-hydroxybenzoate (5) has been identified from the New Zealand liverwort Trichocolea hatcheri (T. hatcheri) on the basis of spectroscopic evidence. This compound was tested for its growth inhibitory effects against tumor cell lines, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. It showed growth inhibition activity against Staphylococcus epidermidis (MIC, 1,000 ${\mu}$g/mL). These results suggest that compound 5 possesses antitumoral , antimicrobial and antioxidative activities.

• 동의생리병리학회지
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• 제18권2호
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• pp.596-598
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• 2004

The effects of water extract from Atractylodes macrocephala Koidz on biological activity were investigated. The crude water extract of A. macrocephala inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (3 mm inhibition zone at 300 ㎍/disc). However, it did not show growth inhibition activity against Sreptococcus mutans JC-2 (MIC >1,000 ㎍/mL). This extract was cytotoxic to P388 murine leukaemia cells ATCC CCL 46 P388D1, (IC/sub 50/ 62.24 ㎍/mL at 150 ㎍/disc). These results suggest that water extract of A. macrocephala possesses antitumoral, and antimicrobial activities.

• Archives of Pharmacal Research
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• 제19권2호
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• pp.91-94
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• 1996

In the course of a search for antitumor agents, we found that the extract of Curcuma longa was effective in inducing apoptosis or programmed cell death (PCD) in human myeloid leukemia cells (HL-60). Active compounds for PCD were isolated from the hexanic extraction of the rhizome of Curcuma longa. With the several chromatographies, and spectral data, they were identified as ar-turmerone and $\beta-atlantone$. The present results demonstrate that the exposure of human myeloid leukemia HL-60 cells to clinically achievable concentrations of arturmerone (TU) or .$\beta-atlantone$(AT) produced internucleosomal DNA fragmentation of approximately 200 base-pair multiples, and the morphological changes characteristic of cells undergoing apoptosis or PCD. This findings suggest that these agents may exert their antitumoral activity, in part, through induction of apoptosis(PCD).

• 대한한방종양학회지
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• 제4권1호
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• pp.71-87
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• 1998

Even though appropriate immune response is necessary for the survival of the individual, excessive or insufficient immune Response might cause autoimmune or allergic disease. So the immune response must be controlled to the degree that is beneficial for the well being of the individual. This study was undertaken to know the effects of Gunleetang Gagambang on the immune system of the mouse. Gunleetang Gagambang has been used for cure of tumor as a traditional medicine without any experimental evidence to support the rational basis for its clinical use. This study was carried out to evaluate the possible therapeutic or antitumoral effects of Gunleetang Gagambang extract against tumor, and to carry out some mechanisms responsible for its effect. Some kinds of tumor were induced by the typical application of 3-methylcholanthrene(MCA) or by the implantation(s.c) of malignant tumor cells such as leukemia cells(3LL cells) or sarcoma cells(S180 cells). Treatment of the Gunleetang Gagambang on water-extract(dailly 1mg/mouse, i. p.) was continued for 7 days prior to tumor induction and after that the treatment was lasted for 20 days. Against squamous cell carcinoma induced by MCA, Gunleetang Gagambang decreased not only the frequency of tumor production but also the number and the weight of tumors per tumor bearing mice(TBM). Gunleetang Gagambang on also significantly suppressed the development of 3LL cell and S180 cell-implanted tumors in occurrence-frequency and their size. and some developed tumors were regressed by the continuous treatment of Gunleetang Gagambang extract into TBM. In vitro, treatment of Gunleetang Gagambang extract had no effect on the growth of some kinds of cell line such as FsaII, A431 strain but significantly inhibited the proliferation of 3LL, S180 cells and augmented the DNA synthesis of mitogen-activated lymphocytes. Gunleetang Gagambang also stimulated the migrative ability of leukocyte, the MIF and IL-2 production of T lymphocytes, but not IL 6 production of B cells. Gunleetang Gagambang administration to mice enhanced NK cells activities. These results demonstrated that Gunleetang Gagambang extract exhibited a significant prophylactic benefits against tumors and its antitumor activity was manifested depending on the type of tumor cells. And these results also suggested that effect of Gunleetang Gagambang might be chiefly due to nonspecitie enhancement of NK cell activities and cell-mediated immune responses.