• BMB Reports
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• 제51권12호
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• pp.654-659
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• 2018

Antioxidant 1 (ATOX1) protein has been reported to exhibit various protective functions, including antioxidant and chaperone. However, the effects of ATOX1 on the inflammatory response has not been fully elucidated. Thus, we prepared cell permeable Tat-ATOX1 and studied the effects on lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13-acetate (TPA)-induced inflammation. Experimental results showed that transduced Tat-ATOX1 protein significantly suppressed LPS-induced intracellular reactive oxygen species (ROS). Also, Tat-ATOX1 protein markedly inhibited LPS- and TPA-induced inflammatory responses by decreasing cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) and further inhibited phosphorylation of mitogen activated protein kinases (MAPKs; JNK, ERK and p38) and the nuclear factor-kappaB ($NF-{\kappa}B$) signaling pathway. These results indicate that the Tat-ATOX1 protein has a pivotal role in inflammation via inhibition of inflammatory responses, suggesting Tat-ATOX1 protein may offer a therapeutic strategy for inflammation.

• 한국가정과학회지
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• 제7권2호
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• pp.31-46
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• 2004

The purpose of this study was to investigate the physiological functionality of Coriolus versicolor Quel (CV) and Ganoderma IUCIdum (Fr.)Karst (GL) by estimate the proximate composition and content of antioxidant components. 1. In the physicochemical property. the content of proximate composition of GL was higher 08.28% of moisture, 10.3% of crude protein, 78.4% of crude fiber) than that of CV, but the content of crude lipid and ashes of GL was higher than that of CV. 2. CV had relatively higher content of antioxidant components such as total phenol, carotenoids and v itamin C. than GL did. Antioxidant mineral components such as magnesium and zinc also had relatively higher in CV than in GL. 3. TBA value, conjugated diene production, LDL oxidation, DPPH of methanol extracts in CV and GL were as high as a-Tocopherol.

• 생명과학회지
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• 제27권11호
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• pp.1299-1307
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• 2017

LOX-1,2,3 결핍과 비소화성 당류인 raffinose와 stachyose 함량이 낮은 품종(LRS)인 진양콩, 하영콩 및 대복콩을 원료로 하여 제조한 두부의 품질특성을 태광콩 두부와 비교하였다. 태광콩과 하영콩의 백립중은 여타 시료에 비해 유의적으로 높았다. 조단백질 함량은 태광콩에 비해 진양콩 및 하영콩에서 유의적으로 많았다. 두부의 수율은 태광콩, 하영콩 및 대복콩으로 만든 두부가 비슷한 수준이었다. 두부의 경도는 태광콩 및 대복콩으로 만든 두부가 가장 낮았으며, 하영콩으로 만든 두부에서 유의적으로 높았다. 콩 및 두부의 이소플라본 함량은 대조구에 비해 LRS 콩은 약 1.4~1.6배, 두부는 약 1.8~3.4배 높은 함량이었다. 콩의 총 페놀 함량은 대조구에 비해 하영콩 및 대복콩에서 유의적으로 높았다. 두부의 총 페놀 및 플라보노이드 함량은 대조구에 비해 하영콩 및 대복콩으로 만든 두부에서 유의적으로 높았다. 콩의 항산화 활성은 태광콩이 LRS 콩에 비해 높은 활성이었으나, 두부에서는 하영콩 및 대복콩으모 만든 두부가 대조구에 비해 유의적으로 높았다. 따라서 LRS 콩은 두부 제조에 적합하나 특히 하영콩이 콩의 단백질, 이소플라본 함량 및 두부의 항산화 활성 측면에서 가장 우수한 것으로 판단된다.

• 동아시아식생활학회지
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• 제24권6호
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• pp.853-861
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• 2014

우리밀과 수입밀로 쿠키, 머핀, 바게트를 제조하여 총 페놀 함량, 항산화 효과, 단백질 소화도를 비교하였다. 또한 쿠키와 머핀 제품에 천년초와 홍화씨를 첨가하여 총 페놀 함량, 항산화 효과 및 단백질 소화도에 미치는 영향을 연구하였다. 천년초의 첨가는 우리밀 쿠키 및 머핀의 80% methanol 추출물에서 총 페놀 함량 및 항산화 효과를 증가시켰으나, 유의적인 차이는 보이지 않았다. 홍화씨 첨가는 우리밀 쿠키의 80% ethanol과 80% methanol 추출물의 총 페놀 함량을 유의적으로 증가시켜 기능성 소재의 첨가가 쿠키의 품질 특성을 향상시키는 결과를 보여주었다. 우리밀과 수입밀로 만든 쿠키, 머핀, 바게트의 80% ethanol 추출물에서 우리밀로 만든 머핀의 총 페놀 함량이 4.53 mg QE/g으로 가장 높게 나타나, 수입밀로 만든 머핀보다 약 1.5배 높게 나타났다. 80% methanol 추출물은 수입밀 바게트가 우리밀 바게트보다 약 1.2배 높게 나타나 용매에 따라 다른 경향을 나타냈다. 80% ethanol 추출물과 80% methanol 추출물의 총 페놀 함량을 비교하면 ethanol 추출물이 모든 시료에서 높은 총 페놀 함량을 보여, 우리밀에서 더 높은 총 페놀 함량을 나타냈다. 우리밀과 수입밀의 ethyl acetate 추출물의 항산화 효과는 유의적인 차이는 나타나지 않았으나, 머핀의 80% methanol 추출물은 우리밀이 수입밀보다 2.4배 가량 높은 항산화 효과를 나타냈다. 그 결과, 추출 용매에 따라 차이는 있으나, 우리밀 제품이 수입밀 제품보다 높은 항산화 효과를 가진다고 추정된다. 단백질 소화도에서 우리밀과 수입밀을 비교해 보면 모든 제품에서 유의적인 차이를 보이지 않았다. 결과적으로 단백질 소화도와 같은 품질 특성은 우리밀이 수입밀의 품질과 유사하며, 항산화 효과와 총 페놀 함량은 우리밀 제품이 우수한 결과를 보여 기능성 면에서 우수한 밀 품종이라고 사료된다.

• BMB Reports
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• 제42권2호
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• pp.113-118
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• 2009

Despite the growing body of evidence suggesting a role for MsrA in antioxidant defense, little is currently known regarding the function of MsrB in cellular protection against oxidative stress. In this study, we overexpressed the mammalian MsrB and MsrA genes in Saccharomyces cerevisiae and assessed their subcellular localization and antioxidant functions. We found that the mitochondrial MsrB3 protein (MsrB3B) was localized to the cytosol, but not to the mitochondria, of the yeast cells. The mitochondrial MsrB2 protein was detected in the mitochondria and, to a lesser extent, the cytosol of the yeast cells. In this study, we report the first evidence that MsrB3 overexpression in yeast cells protected them against $H_2O_2$-mediated cell death. Additionally, MsrB2 overexpression also provided yeast cells with resistance to oxidative stress, as did MsrA overexpression. Our results show that mammalian MsrB and MsrA proteins perform crucial functions in protection against oxidative stress in lower eukaryotic yeast cells.

• 약학회지
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• 제36권5호
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• pp.460-468
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• 1992

Several bichemical parameters related to free radicals were estimated in senile-prone (P) and resistant(R) strains of male senescence-accelerated mice(SAM) at 2, 5 and 11 months of age. The superoxide generation was increased with age in SAM-R/1 and SAM-P/2. Compared to SAM-R/1, more generation of superoxide was significantly noted in the SAM-P/2 liver. The activities of Cu/Zn-superoxide dismutase and catalase were decreased during aging and these activities in SAM-P/2 were significantly lower than in SAM-R/l liver. The activities of glutathione S-transferase were varied with aging, whereas SAM-P/2 showed lower levels compared to SAM-R/l. The gradual decreases of glutathione, protein bound-SH and nonprotein bound-SH contents were noted with increasing age. SAM-P/2 liver contained lesser amounts of glutathione and nonprotein bound-SH compared to SAM-R. In conclusion, superoxide generation was increased whereas the antioxidant enzyme activities were decreased during aging in SAM-R/1. In addition, SAM-P/2 strain showed more superoxide generation and less antioxidant enzyme activities than SAM-R/1 in the liver, thus we assume that these factors might accelerate the senescence of SAM-P/2 strain.

• Fisheries and Aquatic Sciences
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• 제22권1호
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• pp.2.1-2.9
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• 2019

In the present study, the protective effects of Hizikia fusiforme and Chlorella sp. extracts on lead acetate-induced hepatotoxicity were investigated. Hepatic damage was induced in rats by intraperitoneal (i.p.) injection of lead acetate and the protective effects of H. fusiforme (HZK) and Chlorella sp. (CHL) extracts on lead acetate-induced hepatic damage in rat liver were examined. The results revealed significantly increased glutamic oxaloacetate and glutamic pyruvic transaminase levels in the group treated with lead acetate only (Pb group); oral administration of HZK and CHL extracts tended to decrease the enzyme levels similar to those observed in the control group. Regarding antioxidant enzymes, superoxide dismutase activity was increased in the Pb group and decreased in a concentration-dependent manner in the HZK- and CHL-treated groups. Glutathione levels were increased in a concentration-dependent manner in the HZK- and CHL-treated groups. There was no significant difference in catalase activity. Western blot analysis showed inflammation-related protein expression in mitogen-activated protein kinase and Nrf2 pathways was affected in the HZK- and CHL-treated groups. Therefore, HZK and CHL extracts exerted antioxidant and anti-inflammatory effects against lead acetate-induced hepatotoxicity. Development of functional health foods containing HZK and CHL extracts, which have hepatoprotective effects against inhaled lead acetate, should be considered.

• Journal of Korean Biological Nursing Science
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• 제23권3호
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• pp.247-255
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• 2021

Purpose: The goal of this study was to see how different aerobic exercise intensities affected AMP-activated protein kinase (AMPK), reactive oxygen, and antioxidant enzymes in young mice during an 8-week period. Methods: Forty male C57BL/6 mice, aged seven weeks, were randomly assigned to one of four groups: control (n=10), low-intensity exercise (n=10), moderate-intensity exercise (n=10), and high-intensity exercise (n=10). For eight weeks, aerobic activity was performed once a day for 35-40 minutes, five days a week. The data were analyzed using descriptive statistics, analysis of variance (ANOVA), chi-squared tests, and the Tukey test in the SPSS/WIN 25.0 program. Results: Weight (p=.001) was substantially different between the moderate-intensity exercise group and the control group in AMPK (p<.001). In addition, there were no significant differences between the moderate-intensity exercise group and the control group in reactive oxygen malondialdehyde (MDA) levels (p=.136) and antioxidant enzyme superoxide dismutase (SOD) levels (p=.521). Conclusion: These findings suggest that moderate-intensity aerobic exercise increased AMPK activation and helped young mice shed weight.

• Journal of Dairy Science and Biotechnology
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• 제39권1호
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• pp.36-50
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• 2021

This study aimed to evaluate the biological potential of functional food, i.e., specific peptides obtained from the hydrolysis of milk protein, by assessing their antioxidant and antibacterial properties. For the preparation of casein hydrolysates, commercial enzymes were added to 10% casein solution in a 1:200 (w/v) ratio, and samples were collected each hour. Based on the assessment of the degree of hydrolysis (DH) of casein hydrolysates, it was observed that the concentration of all enzymatic hydrolysates increased rapidly from 30 to 40 minutes. However, no change was observed in their concentrations after 150 minutes. Protamex® and Neutrase® exhibited the highest DH when compared to other enzymes. Furthermore, SDS-PAGE was performed for analyzing the proteolytic pattern of each enzyme, except for Flavourzyme®, and peptides in the size range of 20-25 kDa were identified. Subsequently, peptides produced by two enzymes were isolated using a preparative liquid chromatography system. Overall, NF3, NF4, PF5, and PF6 showed higher antioxidant potential than other peptide fractions. Moreover, NF7 and PF3 exhibited the highest antibacterial activity. In this study, we evaluated the biological potential of novel casein-derived peptides that may find application in the food and healthcare industry.

• 한국식생활문화학회지
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• 제38권5호
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• pp.356-364
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• 2023

This study compared the physicochemical properties of soybean curd residue and black soybean curd residue produced by hot air-drying and freeze-drying. Regardless of drying method, the crude protein, crude ash, crude fiber contents, pH, L, a, b color values and water soluble index were higher in soybean curd residue, whereas total polyphenol contents and antioxidant activity were higher in black soybean curd residue. Significant differences in water absorption index, oil absorption capacity and emulsion activity were observed between soybean curd residue and black soybean curd residue in freeze-drying. On the other hand, the emulsion stability was not significant difference in both hot-air drying and freeze-drying. The crude protein and crude fiber contents of soybean curd residue were not significant difference between hot-air drying and freeze-drying. Freeze-drying resulted in higher crude ash contents, pH, water absorption index, water soluble index, oil absorption capacity, emulsion activity and emulsion stability than hot-air drying. Hot-air drying have caused significantly higher water contents, water activity, total polyphenol contents and antioxidant activity in soybean curd residue than freeze-drying. In conclusion, soybean type and drying methods affect the physicochemical and quality characteristics of soybean curd residue, which could be important factors in the manufacture of processed foods.