• 한국식품영양과학회지
• /
• 제42권5호
• /
• pp.675-681
• /
• 2013

기능성 고추장을 제조할 목적으로 브로콜리잎 분말을 첨가하여 고추장을 제조한 후 80% 메탄올, 80% 에탄올, 물로 추출하여 총페놀 함량과 항산화 및 항암활성을 측정하여 재래식 고추장 및 개량식 고추장과 비교하였다. 총페놀 함량은 80% 메탄올과 80% 에탄올 추출물에서 브로콜리 고추장이 각각 524.2와 541.9 mg GAE/100 g으로 재래식 고추장의 431.0과 499.9 mg GAE/100 g보다 각각 22%와 8% 높았다. DPPH radical 소거능은 80% 메탄올 추출물에서는 브로콜리 고추장이 재래식 고추장보다 1.6배 높았으나, 80% 에탄올 추출물에서는 브로콜리 고추장과 재래식 고추장이 비슷하였다. 활성산소 흡수능력은 80% 메탄올과 80% 에탄올 추출물에서 브로콜리 고추장이 재래식 고추장보다 높았으나 유의적인 차이를 나타내지 않았으며 개량식 고추장보다는 각각 55%와 23% 높았다. ACE 저해 활성은 80% 메탄올 추출물에서 브로콜리 고추장은 재래식 고추장과는 유사하였으나 개량식 고추장보다는 2.6배 높았다. 항암활성은 브로콜리 고추장의 80% 에탄올 추출물인 경우 대장암 세포주(HT-29)와 폐암 세포주(NCI-H1229)에서 각각 10.7과 18.3%의 생장 저해효과를 나타내었다. 따라서 브로콜리잎의 건조분말을 첨가하여 재래식 방법으로 제조한 고추장은 총페놀 함량이 높으며, 재래식 고추장이나 공장식 고추장보다 다소 높은 항산화 활성과 암세포 성장 저해효과를 나타내어 브로콜리 분말의 첨가에 의해 고추장의 기능성이 향상되었다.

• 한국식품저장유통학회지
• /
• 제16권4호
• /
• pp.554-561
• /
• 2009

B. subtilis DJI 균주로 접종 후 천일염을 첨가하여 제조한 B. subtilis DJI 된장의 에탄올, 물 및 헥산의 용매별 항산화효과를 비교하였다. B. subtilis DJI 된장의 에탄올 추출물 수율은 15.9%, 헥산 추출물 수율은 14.9% 및 물 추출물 수율은 26.9% 범위로 물 추출물 수율이 가장 높았다. 과산화물가는 B. subtilis DJI 된장 용매별 추출물 중에서 에탄올 추출물이 300 ppm에서 600 ppm으로 농도를 증가하였을 경우 저하되어 과산화물 생성 억제효과가 있었으며, 합성항산화제인 BHT와 유사한 경향을 보였다. 아질산염 소거능은 B. subtilis DJI 된장 에탄올 추출물이 300 ppm과 600 ppm 농도에서 각각 18.26%와 23.36%로 가장 높았고, 물 추출물에서 10.23%와 12.46%였고, 헥산 추출물에서는 2.62%와 5.51%로 가장 낮아 헥산 추출물의 경우 항산화효과가 아주 미비한 것으로 나타났다. 전자공여능도 아질산염 소거능과 비슷한 경향을 보여 시료 농도가 증가할수록 항산화효과가 증가하였고, 에탄올 추출물이 가장 우수한 항산화효과를 나타내었다. 그러나 B. subtilis DJI 된장 추출물의 아질산염 소거능과 전자공여능은 합성항산화제인 BHT에 비해서는 낮은 경향이었다. Rancimat 방법으로 측정한 항산화효과도 두 농도 모두 B. subtilis DJI 된장 에탄올 추출물의 항산화효과가 가장 우수하였으나, 물과 헥산 추출물은 항산화효과에 영향을 미치지 않았다.

• 한국자원식물학회지
• /
• 제31권4호
• /
• pp.312-321
• /
• 2018

Angelica tenuissima, also known as Ligusticum tenuissimum, is classified as a food-related plant and has been used as traditional medicines treating headache and anemia in Asia. However, its anti-melanogenic effect has not been reported in detail. When the extract of Angelica tenuissima (ATE) was prepared by the extraction with 70% EtOH at $80^{\circ}C$ (final yield = 22%), the contents of decursin and Z-ligustilide in ATE were determined 0.06% and 8.43%, respectively. Total flavonoid and phenolic content in mg ATE were $5.52{\pm}0.07{\mu}g$ quercetin equivalents and $237.27{\pm}13.24{\mu}g$ gallic acid equivalents, respectively. Antioxidant capacity of ATE determined by DPPH and ABTS assay was increased with a dose dependent manner up to $1000{\mu}g/m{\ell}$. The amount of melanin synthesis followed by ${\alpha}-melanocyte$ stimulating hormone on B16F10 cells were significantly reduced in the presence of ATE (250 to $1000{\mu}g/m{\ell}$, p<0.05). ATE (125 to $1000{\mu}g/m{\ell}$, p<0.05) suppressed the tyrosinase activity but did not show any significant effect on ${\alpha}-glucosidase$ activity at the same condition. Taken together, ATE possesses tyrosinase inhibitory potential with significant antioxidant capacities. These effects of ATE might be involved in suppression of melanin synthesis, at least, in B16F10 cells. The anti-melanogenic potential of ATE will provide an insight into developing a new skin whitening product.

• 한국식품과학회지
• /
• 제50권6호
• /
• pp.660-664
• /
• 2018

본 연구에서 이용한 피나무 꿀, 헛개나무 꿀, 밤나무 꿀의 EAF는 마누카 꿀 EAF 보다 높은 산화방지능 및 타이로시네이스 억제능을 보였다. 특히 밤나무 꿀의 EAF는 양성대조군인 고지산에 버금가는 타이로시네이스 억제능을 보였다. 5가지 화종 유래 꿀의 EAF ($100{\mu}g/mL$) 처리시 B16F1 세포에서 멜라닌 생성 억제능은 밤나무 꿀>헛개나무 꿀>마누카 꿀>피나무 꿀>아카시아 꿀의 순서대로 낮아졌다. B16F1 세포 내 타이로시네이스의 효소활성 억제의 경우 5종류 꿀 EAF 중에서 밤나무 꿀 EAF ($100{\mu}g/mL$) 처리시 가장 높았다. 본 연구에서는 다양한 화종의 국내산 꿀에 대한 생리활성소재 탐색을 통하여 산화방지능 및 멜라닌 생성 억제 효과를 확인하였으며, 이를 통해 미백활성 소재로서 기능성 식품 및 화장품 산업으로의 적용 가능성을 제시하였다.

• Journal of Microbiology and Biotechnology
• /
• 제28권1호
• /
• pp.22-31
• /
• 2018

Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at $37^{\circ}C$. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to $500{\mu}g/ml$ of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL ($100{\mu}g/ml$) could highly suppress the content of melanin and melanosome formation in B16-F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.

• Natural Product Sciences
• /
• 제21권1호
• /
• pp.6-13
• /
• 2015

Phytochemical investigation of the aerial components of Ducrosia ismaelis Asch. led to the isolation of six known compounds, psoralen (1), isopsoralen (2), cnidioside A (3), (-)-syringaresinol-O-${\beta}$-D-glucopyranoside (4), (E)-plicatin B (5), trilinolein (6). The chemical structures of these compounds were elucidated from spectroscopic data and by comparison of these data with previously published results. The antioxidant, anti-osteoporotic and cardiovascular related activities of the isolated compounds were assessed using oxygen radical absorbance capacity (ORAC), reducing capacity, tartrate-resistant acid phosphatase (TRAP), and soluble epoxide hydrolase (sEH) inhibitory activity assays. Compounds (3-5) showed potent peroxyl radical-scavenging capacities with ORAC values of $11.06{\pm}0.39$, $7.98{\pm}0.10$, and $13.99{\pm}0.06$ Trolox equivalent (TE) at concentrations of $10{\mu}M$, respectively. Only compounds 4 and 5 was able to significantly reduce $Cu^{2+}$ ions, with a reduction value of $9.06{\pm}0.32$ and $4.61{\pm}0.00{\mu}M$ Trolox Equivalent (TE) at a concentration of $10{\mu}M$. Compound 5 at $10{\mu}M$ exhibited a potent inhibitory effect on osteoclastic TRAP activity with a TRAP value of $86.05{\pm}6.55%$ of the control. Compounds 1, 3 and 5 potently inhibited sEH activity with $IC_{50}$ values of 41.6 4.9, 16.0 1.1, and 49.0 $5.7{\mu}M$, respectively.

• Journal of Microbiology and Biotechnology
• /
• 제30권2호
• /
• pp.163-171
• /
• 2020

Brugmansia arborea L. (Solanaceae), commonly known as "angel's trumpet," is widely grown in North America, Africa, Australia, and Asia. It has been mainly used for ornamental purposes as well as analgesic, anti-rheumatic, vulnerary, decongestant, and anti-spasmodic materials. B. arborea is also reported to show anti-cholinergic activity, for which many alkaloids were reported to be principally responsible. However, to the best of our knowledge, a phytochemical study of B. arborea flowers has not yet been performed. Four flavonol glycosides (1-4) and one dihydroflavanol (5) were for the first time isolated from B. arborea flowers in this study. The flavonoids showed significant antioxidant capacities, suppressed nitric oxide production in lipopolysaccharide (LPS)-treated RAW 264.7 cells, and reduced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) protein production increased by LPS treatment. The contents of compounds 1-4 in n-BuOH fraction were determined to be 3.8 ± 0.9%, 2.2 ± 0.5%, 20.3 ± 1.1%, and 2.3 ± 0.4%, respectively, and that of compound 5 in EtOAc fraction was determined to be 12.7 ± 0.7%, by HPLC experiment. These results suggest that flavonol glycosides (1-4) and dihydroflavanol (5) can serve as index components of B. arborea flowers in standardizing anti-inflammatory materials.

• 한국식품영양과학회지
• /
• 제45권1호
• /
• pp.149-154
• /
• 2016

현미와 홍국발효 콩 시료의 첨가량을 달리하여 제조한 식초의 미용건강기능성 소재(cosmeceuticals)로의 활용 가능성을 평가한 결과는 다음과 같다. DPPH와 ABTS 라디칼 소거능으로 측정한 항산화 활성은 현미 식초에 비해 홍국발효 콩이 첨가된 시료가 첨가량에 비례하여 최대 7배 이상 증가하였다(P<0.05). 특히 tyrosinase 저해 활성은 50% 첨가량의 시료에서 kojic acid의 저해 활성과 유사하게 나타나 미백 소재로서의 가능성을 보였으며, elastase 저해 활성 역시 첨가량에 비례하여 유의적으로 증가하였다(P<0.05). 현미 식초에 비해 홍국발효 콩을 1:1(w/w)의 비율로 첨가한 식초의 높은 생리활성은 시료에 함유된 총 페놀 함량, 특히 이소플라본 비배당체형의 genistein과 daidzein의 농도와 높은 상관관계가 성립되었다.

• 생약학회지
• /
• 제51권1호
• /
• pp.70-77
• /
• 2020

Mentha arvensis is used traditional medicine to treat various disorders. In the present study, M. arvensis were extracted by the solid-phase microextraction (SPME) method and analyzed by gas chromatograph-mass spectrometry (GC-MS). We investigated the protective effects and mechanisms of a M. arvensis extract on scopolamine-induced cognitive and memory impairment. Mice were orally pretreated with a M. arvensis extract or normal saline, and then behavior tests were conducted 30 min after scopolamine injection. The antioxidant capacities were analyzed by free radical scavenging (DPPH and ABTS). Acetylcholinesterase (AChE) activity were also measured using Ellman's method ex vivo test. In behavior tests, percent of spontaneous alteration, escape latency and swimming time in target quadrant were improved by the administration of the M. arvensis extract, which suggests that the M. arvensis extract improves memory function in the scopolamine-treated mice model. In addition, M. arvensis extract showed inhibition of the free radical and AChE activity. The results of the present study suggest that the M. arvensis extract ameliorates scopolamine-induced cognitive and memory deficits through the inhibition of free radicals and AChE activity. Therefore, M. arvensis may be a promising neuroprotective agent for management of learning and memory improvements in human dementia patients.

• Journal of Microbiology and Biotechnology
• /
• 제31권4호
• /
• pp.584-591
• /
• 2021

Marine algae (seaweed) encompass numerous groups of multicellular organisms with various shapes, sizes, and colors, and serve as important sources of natural bioactive substances. The brown alga Ecklonia cava Kjellman, an edible seaweed, contains many bioactives such as phlorotannins and fucoidans. Here, we evaluated the antioxidative, neuroprotective, and anti-apoptotic effects of E. cava extract (ECE), E. cava phlorotannin-rich extract (ECPE), and the phlorotannin dieckol on neuronal PC-12 cells. The antioxidant capacities of ECPE and ECE were 1,711.5 and 1,050.4 mg vitamin C equivalents/g in the ABTS assay and 704.0 and 474.6 mg vitamin C equivalents/g in the DPPH assay, respectively. The dieckol content of ECPE (58.99 mg/g) was approximately 60% higher than that of ECE (36.97 mg/g). Treatment of PC-12 cells with ECPE and ECE increased cell viability in a dose-dependent manner. Intracellular oxidative stress in PC-12 cells due to ECPE and ECE decreased dose-independently by up to 63% and 47%, respectively, compared with the stress control (323%). ECPE reduced the production of the pro-apoptotic proteins Bax and caspase-3 more effectively than ECE. Early and late apoptosis in PC-12 cells were more effectively decreased by ECPE than ECE treatments. From the results obtained in this study, we concluded that ECPE, which is rich in phlorotannins, including the marker compound dieckol, may be applied to the development of functional materials for improving cognition and memory.